An Analytic Method for SS-Expansion involving Resonance and Reduction

In this paper we describe an analytic method able to give the multiplication table(s) of the set(s) involved in an $S$-expansion process (with either resonance or $0_S$-resonant-reduction) for reaching a target Lie (super)algebra from a starting one, after having properly chosen the partitions over subspaces of the considered (super)algebras. This analytic method gives us a simple set of expressions to find the partitions over the set(s) involved in the process. Then, we use the information coming from both the initial (super)algebra and the target one for reaching the multiplication table(s) of the mentioned set(s). Finally, we check associativity with an auxiliary computational algorithm, in order to understand whether the obtained set(s) can describe semigroup(s) or just abelian set(s) connecting two (super)algebras. We also give some interesting examples of application, which check and corroborate our analytic procedure and also generalize some result already presented in the literature.Comment: v3, 47 pages, misprints corrected in Fortschritte der Physik, Published online 7 November 201

Lean Manufacturing Production Method using the Change Management Approach to Reduce Backorders at SMEs in the Footwear Industry in Peru

This article proposes a production method that aims to increase the manufacturing capacity of a footwear small- and medium-sized enterprise (SME) to reduce backorders. Therefore, an assessment is carried out and delays in production processes, excess product transport time, defective products, and inefficient work methods are identified. This article proposes designing a Lean manufacturing method using the change management approach, whose methodology is composed of six phases. In phase 0, change management is carried out; in phase 1, the company's current situation is reviewed using the Value Stream Mapping (VSM); in phase 2, the work area is reorganized (implementing SLP and 5S); in phase 3, production is balanced (implementing Line Balancing); in phase 4, continual improvement is established using the Kaizen tool; and finally, in phase 5, the results are evaluated. Through validation, it was possible to confirm that Lean manufacturing tools along with change management increased order deliveries by 82%

Xenopus laevis and Emerging Amphibian Pathogens in Chile

Amphibians face an extinction crisis with no precedence. Two emerging infectious diseases, ranaviral disease caused by viruses within the genus Ranavirus and chytridiomycosis due to Batrachochytrium dendrobatidis (Bd), have been linked with amphibian mass mortalities and population declines in many regions of the globe. The African clawed frog (Xenopus laevis) has been indicated as a vector for the spread of these pathogens. Since the 1970s, this species has been invasive in central Chile. We collected X. laevis and dead native amphibians in Chile between 2011 and 2013. We conducted post-mortem examinations and molecular tests for Ranavirus and Bd. Eight of 187 individuals (4.3 %) tested positive for Ranavirus: seven X. laevis and a giant Chilean frog (Calyptocephallela gayi). All positive cases were from the original area of X. laevis invasion. Bd was found to be more prevalent (14.4 %) and widespread than Ranavirus, and all X. laevis Bd-positive animals presented low to moderate levels of infection. Sequencing of a partial Ranavirus gene revealed 100 % sequence identity with Frog Virus 3. This is the first report of Ranavirus in Chile, and these preliminary results are consistent with a role for X. laevis as an infection reservoir for both Ranavirus and Bd

Chytridiomycosis outbreak in a Chilean giant frog (Calyptocephalella gayi) captive breeding program: genomic characterization and pathological findings

Emerging infectious diseases in wildlife are increasingly associated with animal mortality and species declines, but their source and genetic characterization often remains elusive. Amphibian chytridiomycosis, caused by the fungus Batrachochytrium dendrobatidis (Bd), has been associated with catastrophic and well-documented amphibian population declines and extinctions at the global scale. We used histology and whole-genome sequencing to describe the lesions caused by, and the genetic variability of, two Bd isolates obtained from a mass mortality event in a captive population of the threatened Chilean giant frog (Calyptocephalella gayi). This was the first time an association between Bd and high mortality had been detected in this charismatic and declining frog species. Pathological examinations revealed that 30 dead metamorphosed frogs presented agnathia or brachygnathia, a condition that is reported for the first time in association with chytridiomycosis. Phylogenomic analyses revealed that Bd isolates (PA1 and PA2) from captive C. gayi group with other Bd isolates (AVS2, AVS4, and AVS7) forming a single highly supported Chilean Bd clade within the global panzootic lineage of Bd (BdGPL). These findings are important to inform the strengthening of biosecurity measures to prevent the impacts of chytridiomycosis in captive breeding programs elsewhere

Regulation and distribution of European sea bass perforins point to their role in the adaptive cytotoxic response against NNV

© 2023. The authors. This document is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by /4.0/ This document is the accepted version of a published work that appeared in final form in Fish and Shellfish Immunology.Cell-mediated cytotoxicity is a complex immune mechanism that involves the release of several killing molecules, being perforin (PRF) one of the most important effector players. Perforin is synthesized by T lymphocytes and natural killer cells in mammals and responsible for the formation of pores on the target cell membrane during the killing process. Although perforin has been extensively studied in higher vertebrates, this knowledge is very limited in fish. Therefore, in this study we have identified four prf genes in European sea bass (Dicentrarchus labrax) and evaluated their mRNA levels. All sea bass prf genes showed the typical and conserved domains of its human orthologue and were closely clustered by the phylogenetic analysis. In addition, all genes showed constitutive and ubiquitous tissular expression, being prf1.9 gene the most highly expressed in immune tissues. Subsequently, in vitro stimulation of head-kidney (HK) cells with phytohemagglutinin, a T-cell activator, showed an increase of all prf gene levels, except for prf1.3 gene. European sea bass HK cells increased the transcription of prf1.2 and prf1.9 during the innate cell-mediated cytotoxic activity against xenogeneic target cells. In addition, sea bass infected with nodavirus (NNV) showed a similar expression pattern of all prf in HK and brain at 15 days post-infection, except for prf1.3 gene and in the gonad. Finally, the use of a polyclonal antibody against PRF1.9 showed an increase of positive cells in HK, brain and gonad from NNV-infected fish. Taken together, the data seem to indicate that all prf genes, except prf1.3, appear to be involved in the European sea bass immunity, and probably in the cell-mediated cytotoxic response, with PRF1.9 playing the most important role against nodavirus. The involvement of the PRFs and the CMC activity in the vertical transmission success of the virus is also discussed

Synthesis of Batrachochytrium dendrobatidis infection in South America: amphibian species under risk and areas to focus research and disease mitigation

Amphibian chytridiomycosis, caused by Batrachochytrium dendrobatidis (Bd), has been recognized as the infectious disease causing the most catastrophic loss of biodiversity known to science, with South America being the most impacted region. We tested whether Bd prevalence is distributed among host taxonomy, ecoregion, conservation status and habitat preference in South America. Here we provide a synthesis on the extent of Bd infection across South America based on 21 648 molecular diagnostic assays, roles of certain species in the epidemiology of Bd and explore its association with the reported amphibian catastrophic declines in the region. We show that Bd is widespread, with a continental prevalence of 23.2%. Its occurrence in the region shows a phylogenetic signal and the probability of infection is determined by ecoregion, preferred habitat and extinction risk hosts' traits. The taxa exhibiting highest Bd occurrence were mostly aquatic amphibians, including Ranidae, Telmatobiidae, Hylodidae, Calyptocephalellidae and Pipidae. Surprisingly, families exhibiting unusually low Bd prevalence included species in which lethal chytridiomycosis and population declines have been described (genera Atelopus, Rhinoderma and Eleutherodactylus). Higher than expected prevalence of Bd occurred mainly in amphibians living in association with mountain environments in the Andes and Atlantic forests, reflecting highly favourable Bd habitats in these areas. Invasive amphibian species (e.g. Lithobates catesbeianus and Xenopus laevis) exhibited high Bd prevalence; thus we suggest using these as sentinels to understand their potential role as reservoirs, vectors or spreaders of Bd that can be subjected to management. Our results guide on the prioritization of conservation actions to prevent further biodiversity loss due to chytridiomycosis in the world's most amphibian diverse region

Differentiating Endurance- and Speed-Adapted Types of Elite and World Class Milers According to Biomechanical, Pacing and Perceptual Responses during a Sprint Interval Session

The aim was to compare pacing, biomechanical and perceptual responses between elite speed- and endurance-adapted milers during a sprint interval training session (SIT). Twenty elite and world-class middle-distance runners (male: n = 16, female: n = 4; 24.95 ± 5.18 years; 60.89 ± 7 kg) were classified as either speed- or endurance-adapted milers according to their recent per-formances at 800 m or longer races than 1500 m (10 subjects per group). Participants performed 10 repetitions of 100 m sprints with 2 min of active recovery between each, and performance, per-ceptual and biomechanical responses were collected. The difference between accumulated times of the last and the first five repetitions was higher in speed-adapted milers (ES = 1.07) displaying a more positive pacing strategy. A higher coefficient of variation (CV%) was displayed across the session by speed-adapted milers in average repetition time, contact time, and affective valence (ES ≥ 1.15). Speed-adapted milers experienced lower rates of valence after the 4th repetition excepting at the 8th repetition (ES ≥ 0.99). Speed-adapted milers may need to display a more positive pacing profile than endurance-adapted milers and, therefore, would experience lower levels of affective valence and a more rapid increase of ground contact time during a SIT

Catálogo de especímenes tipo del Herbario Nacional del Ecuador (QCNE), Museo Ecuatoriano de Ciencias Naturales

The type-specimen collection of the Herbario Nacional del Ecuador (QCNE) preserves important vouchers from across Ecuador collected by Ecuadorian and foreign researchers. Types are of paramount importance to understad biodiversity because their designation is a requirement for the description of new species. We present a synopsis of the type-specimen collection and a catalogue of Ecuadorian primary types (holotypes, isotypes, neotypes and isoneotypes) held at the QCNE herbarium. In total, 1890 QCNE specimens are designated as nomenclatural types, including 206 holotypes, 632 isotypes, two neotypes and isoneotypes, eight clonotypes, and 1040 paratypes of 828 species, 13 subspecies, six varieties, and one form in 101 families and 296 genera. The catalogue of primary types includes 786 specimens, of which 763 are Angiosperms, 21 Pteridophytes and two Bryophytes, 50, 38 % of them endemic to Ecuador.La colección de especímenes tipo del Herbario Nacional del Ecuador (QCNE) preserva importantes ejemplares testigo procedentes de todo el Ecuador colectados por investigadores ecuatorianos y extranjeros. Los tipos son de gran importancia para entender la biodiversidad porque su designación es un requisito para la descripción de nuevas especies. Presentamos una sinopsis de la colección de especímenes tipo y un catálogo de los tipos primarios ecuatorianos (holotipos, isotipos, neotipos e isoneotipos) depositados en el herbario QCNE. En total, 1890 especímenes QCNE están designados como tipos nomenclaturales, incluyendo 206 holotipos, 632 isotipos, dos neotipos e isoneotipos, ocho clonotipos, y 1040 paratipos de 828 especies, 13 subespecies, seis variedades y una forma en 101 familias y 296 géneros. El catálogo de tipos primarios incluye 786 especímenes, de los cuales 763 son Angiospermas, 21 Pteridofitas y dos Briofitas; 50, 38 % de ellos endémicos del Ecuador