Owyhee Russet: AVariety with High Yields of U.S. No. 1 Tubers, Excellent Processing Quality, and Moderate Resistance to Fusarium Dry Rot (\u3ci\u3eFusarium solani var. coeruleum\u3c/i\u3e)

Owyhee Russet (AO96160-3) originated from a cross between A89384-10 and A89512-3 in 1996. Owyhee Russet was released in 2009 by Oregon State University, in cooperation with the USDA-ARS and the Agricultural Experiment Stations of Idaho and Washington and is a product of the Northwest Potato Variety (Tri-State) Development Program. Owyhee Russet has semi-erect medium sized vines with medium to late maturity. The tubers are long, with a tan skin, medium russeting, and attractive tuber appearance for fresh market. Owyhee Russet was evaluated in several locations across the Northwest for more than 15 years. Total yield of Owyhee Russet is similar to that of Russet Burbank and Ranger Russet but significantly higher than Russet Norkotah. U.S. No.1 tuber yield of Owyhee Russet is significantly higher than Russet Burbank and Russet Norkotah, resulting in substantially higher marketable yield. Owyhee Russet tubers have significantly higher specific gravity than Russet Burbank and Russet Norkotah. Fry color following tuber storage at 4°C and 9°C is significantly lighter for Owyhee Russet than the comparison varieties. Relative strengths include high yield with a very high proportion of U.S. No.1 tubers, good tuber appearance and excellent processing quality, resistance to cold sweetening, common scab and Fusarium dry rot. Weaknesses include susceptibility to foliar and tuber late blight and susceptibility to metribuzin herbicide injury. Allelic patterns of five SSR markers have shown that Owyhee Russet has a distinctive DNA genetic fingerprint from its russet type reference varieties which are Ranger Russet, Russet Burbank, and Russet Norkotah

Stability and Broad-Sense Heritability of Mineral Content in Potato: Potassium and Phosphorus

In the study of nutritional variability in potato it is desirable to know the present range of expression and genetic potential for increase. Potato breeding lines and varieties in two separate trials were evaluated for potassium and phosphorus content by wet ashing and Inductively Coupled Argon Plasma Emission Spectrophotometer analysis. Stability and broad-sense heritability were determined. Among genotypes, potassium content ranged from 1.85 and 2.49% DW while phosphorus content ranged from 0.16 to 0.34% DW over both trials. Genotype by environment interactions were significant in the Tri-State and Western Regional Red/Specialty (WR-R/SP) trials for both potassium and phosphorus, while environments were not. Genotype was a significant source of variation for both minerals in the WR-R/SP trial only. In the Tri-State trials, 7 and 4 of ten clones were unstable before and after removal of environmental heterogeneity, respectively, for potassium content, and 5 and 4 genotypes were unstable before and after removal of environmental heterogeneity, respectively, for phosphorus. In the WR-R/SP Trials, 7 and 3 of 13 clones were unstable before and after removal of environmental heterogeneity, respectively, for potassium content, and 3 and 4 genotypes were unstable before and after removal of environmental heterogeneity, respectively, for phosphorus. Broad sense heritability was low for both potassium and phosphorus in the Tri-State Russet-Skin Trials but high for both potassium and phosphorus in the WR-R/SP Trials. Although potato is a minor contributor of phosphorus to the human diet, it is an important source of potassium. Adult males and females receive 12% of the Recommended Dietary Allowance of potassium from 100 g of potato. Estimates of broad-sense heritability from these two trials suggest that genotypes with higher levels of both potassium and phosphorus can be selected from within the Red/Specialty market class, but not from within the Tri-State russet class. An increase in potassium content in the potato, for which the daily need in the human body is so high, could be a boon to human health.Keywords: Germplasm, Breeding, ICAPES, Human nutrition, RD

Palisade Russet: A Late Blight Resistant Potato Cultivar Having a Low Incidence of Sugar Ends and High Specific Gravity

Palisade Russet is a medium-late maturing, lightly russeted potato cultivar notable for its resistance to late blight (Phytophthora infestans) infection of foliage and tuber. Palisade Russet is suitable for processing with low tuber glucose concentrations observed following long-term storage at a temperature as low as 5.6A degrees C with consistently acceptable fry color scores (USDA value a parts per thousand currency sign2.0) following storage at temperatures of 5.6 to 8.9A degrees C. Reducing sugars are also maintained uniformly throughout the tuber, resulting in a low incidence of sugar ends in French fries relative to standard processing cultivars such as Russet Burbank and Ranger Russet. Palisade Russet has shown high specific gravities in western production regions of the U.S. which may limit its acceptance by the western processing industry. However, in potato production regions with inherently low specific gravities, Palisade Russet could have potential as a processing cultivar. In full-season trials conducted in Idaho, Oregon, and Washington over a 5 year period, the average total yield of Palisade Russet across all three states was very similar to yields observed for Ranger Russet and Russet Burbank. However, the U.S. No. 1 yield of Palisade Russet was 3% and 31% higher relative to Ranger Russet and Russet Burbank, respectively. In addition to late blight, Palisade Russet is also resistant to Verticillium wilt, black dot, and pink rot, and has a moderate level of resistance to net necrosis, PVY, and early blight of both the foliage and tuber. The disease resistances of Palisade Russet make it a good candidate for organic production, or for use by growers seeking reduced pesticide inputs. Palisade Russet displays a low incidence of second growth and growth cracks, especially relative to Russet Burbank, and is intermediate between Ranger Russet and Russet Burbank for incidence of hollow heart/brown center. Blackspot bruise expression for Palisade Russet is less pronounced than for either Ranger Russet or Russet Burbank, however it is slightly more susceptible to shatter bruise. Palisade Russet was released in 2011 by the USDA-ARS and the Agricultural Experiment Stations of Idaho, Oregon, and Washington, and is a product of the Pacific Northwest Potato Variety (Tri-State) Development Program.Keywords: Disease, Variety, Breeding, Foliar, Solanum tuberosumKeywords: Disease, Variety, Breeding, Foliar, Solanum tuberosu

Stability and Broad-Sense Heritability of Mineral Content in Potato: Copper and Sulfur

Sulfur and copper are important for human health. Sulfur deficiency is rare, but may occur in the elderly. However, a large percentage of the U.S. population is deficient in copper. The purpose of this study was to determine the range of values for sulfur and copper available in advanced potato germplasm and varieties and estimate how much genetic variation exists for these two elements. Potato breeding lines and varieties in three multisite trials were evaluated for copper and sulfur content by wet ashing and Inductively Coupled Argon Plasma Emission Spectrophotometer analysis. Stability and broad-sense heritability were determined. Among genotypes, copper content ranged from 2.0 to 4.5 ug-g⁻¹ DW. This was a 2.25-fold difference. In these three trials, environment was never significant, while genotype by environment interactions were always significant. Genotype was significant in two of the regional trials. Broad-sense heritabilities were estimated to be 0.0, 0.93 and 0.51 for the Tri-State, Western Regional Russet and Western Regional Red/Specialty trials, respectively. Among genotypes, sulfur content ranged from 991 to 1488 ug-g⁻¹ DW. The highest value was 50% higher than the lowest. In these three trials, environment was never significant, while genotype x environment interactions were always significant. Genotype was significant in two of the regional trials. Broad-sense heritabilities were estimated to be 0.53, 0.68 and 0.88, for Tri-State, Western Regional Russet, and Western Regional Red/Specialty trials, respectively. For both sulfur and copper, selection in the Western Regional Russet and Western Regional Red/Specialty trials is likely to lead to an increase in content. Selection for sulfur in the Tri-State would result in a gain as well. These results suggest that genetic improvements could be made to potato to enhance the concentrations of these minerals.Keywords: RDA, ICAPES, Germplasm, Human nutrition, BreedingKeywords: RDA, ICAPES, Germplasm, Human nutrition, Breedin

Owyhee Russet: A Variety with High Yields of U.S. No. 1 Tubers, Excellent Processing Quality, and Moderate Resistance to Fusarium Dry Rot (Fusarium solani var. coeruleum)

Owyhee Russet (AO96160-3) originated from a cross between A89384-10 and A89512-3 in 1996. Owyhee Russet was released in 2009 by Oregon State University, in cooperation with the USDA-ARS and the Agricultural Experiment Stations of Idaho and Washington and is a product of the Northwest Potato Variety (Tri-State) Development Program. Owyhee Russet has semi-erect medium sized vines with medium to late maturity. The tubers are long, with a tan skin, medium russeting, and attractive tuber appearance for fresh market. Owyhee Russet was evaluated in several locations across the Northwest for more than 15 years. Total yield of Owyhee Russet is similar to that of Russet Burbank and Ranger Russet but significantly higher than Russet Norkotah. U.S. No.1 tuber yield of Owyhee Russet is significantly higher than Russet Burbank and Russet Norkotah, resulting in substantially higher marketable yield. Owyhee Russet tubers have significantly higher specific gravity than Russet Burbank and Russet Norkotah. Fry color following tuber storage at 4°C and 9°C is significantly lighter for Owyhee Russet than the comparison varieties. Relative strengths include high yield with a very high proportion of U.S. No.1 tubers, good tuber appearance and excellent processing quality, resistance to cold sweetening, common scab and Fusarium dry rot. Weaknesses include susceptibility to foliar and tuber late blight and susceptibility to metribuzin herbicide injury. Allelic patterns of five SSR markers have shown that Owyhee Russet has a distinctive DNA genetic fingerprint from its russet type reference varieties which are Ranger Russet, Russet Burbank, and Russet Norkotah.Keywords: Breeding, Processing, Common scab resistance, Fusarium dry rot resistance, Variety, Solanum tuberosu

Stability and Broad-Sense Heritability of Mineral Content in Potato: Calcium and Magnesium

Calcium and magnesium are two minerals that play prominent roles in animal and plant metabolism. The purpose of this study was to determine if genetic variation exists among advanced potato breeding clones for tuber calcium and magnesium content and the extent of genotype x environment interactions on these two traits. Ten, 13, and 13 clones were evaluated in the Tri-State, Western Regional, and Western Regional Red/Specialty Trials, respectively. Tuber calcium content ranged from 266 to 944 μg-g⁻¹ DW; magnesium from 787 to 1,089 μg-g⁻¹ DW. Genotype x environment interactions were significant in all trials. However, only the Tri-State for calcium and the Western Regional Red/Specialty trials for both minerals displayed a significant source of variation for genotypes. Broad-sense heritabilities for tuber calcium content were 0.65, 0.37 and 0 in the Tri-State, Western Regional, and Western Regional Red/Specialty Trials, respectively. Broad-sense heritabilities for tuber magnesium content were 0.57, 0, and 0.72 in the Tri-State, Western Regional, and Western Regional Red/Specialty Trials, respectively. Potato is not a rich source of either calcium or magnesium for the human diet, but genetic variation exists among potato clones that might be useful for plant health.This is the publisher’s final pdf. The article is copyrighted by the Potato Association of America and published by Springer. It can be found at: http://link.springer.com/journal/12230?nullKeywords: Solanum tuberosum, Biofortification, Genotype x environment interaction, ICAEPS, BreedingKeywords: Solanum tuberosum, Biofortification, Genotype x environment interaction, ICAEPS, Breedin

Alpine Russet: A Potato Cultivar Having Long Tuber Dormancy Making it Suitable for Processing from Long-term Storage

Alpine Russet is a later maturing, oblong-long, lightly russeted potato cultivar, notable for having tuber dormancy comparable to Russet Burbank. Processing quality of Alpine Russet from long-term storage is superior to Russet Burbank, with low percent reducing sugars and uniform fry color due to a low percentage difference of sugars between the bud and stem ends. Alpine Russet yields were comparable to Russet Burbank in early harvest trials and were comparable or significantly larger in late harvest trials depending on the location. At two late season locations, Alpine Russet had the largest total and percent No. 1 yields and the largest percent mid-range No. 1 tubers compared to Ranger Russet and Russet Burbank. It has moderately high specific gravity and is resistant to most external and internal defects. Alpine Russet has been evaluated in public and industry trials throughout the Western U.S. for over 15 years

A major QTL controlling apple skin russeting maps on the linkage group 12 of 'Renetta Grigia di Torriana'

Background: Russeting is a disorder developed by apple fruits that consists of cuticle cracking followed by the replacement of the epidermis by a corky layer that protects the fruit surface from water loss and pathogens. Although influenced by many environmental conditions and orchard management practices, russeting is under genetic control. The difficulty in classifying offspring and consequent variable segregation ratios have led several authors to conclude that more than one genetic determinant could be involved, although some evidence favours a major gene (Ru). Results: In this study we report the mapping of a major genetic russeting determinant on linkage group 12 of apple as inferred from the phenotypic observation in a segregating progeny derived from 'Renetta Grigia di Torriana', the construction of a 20 K Illumina SNP chip based genetic map, and QTL analysis. Recombination analysis in two mapping populations restricted the region of interest to approximately 400 Kb. Of the 58 genes predicted from the Golden Delicious sequence, a putative ABCG family transporter has been identified. Within a small set of russeted cultivars tested with markers of the region, only six showed the same haplotype of 'Renetta Grigia di Torriana'. Conclusions: A major determinant (Ru_RGT) for russeting development putatively involved in cuticle organization is proposed as a candidate for controlling the trait. SNP and SSR markers tightly co-segregating with the Ru_RGT locus may assist the breeder selection. The observed segregations and the analysis of the 'Renetta Grigia di Torriana' haplotypic region in a panel of russeted and non-russeted cultivars may suggest the presence of other determinants for russeting in apple

Expression of ABC Efflux Transporters in Placenta from Women with Insulin-Managed Diabetes

Drug efflux transporters in the placenta can significantly influence the materno-fetal transfer of a diverse array of drugs and other xenobiotics. To determine if clinically important drug efflux transporter expression is altered in pregnancies complicated by gestational diabetes mellitus (GDM-I) or type 1 diabetes mellitus (T1DM-I), we compared the expression of multidrug resistance protein 1 (MDR1), multidrug resistance-associated protein 2 (MRP2) and the breast cancer resistance protein (BCRP) via western blotting and quantitative real-time polymerase chain reaction in samples obtained from insulin-managed diabetic pregnancies to healthy term-matched controls. At the level of mRNA, we found significantly increased expression of MDR1 in the GDM-I group compared to both the T1DM-I (p<0.01) and control groups (p<0.05). Significant changes in the placental protein expression of MDR1, MRP2, and BCRP were not detected (p>0.05). Interestingly, there was a significant, positive correlation observed between plasma hemoglobin A1c levels (a retrospective marker of glycemic control) and both BCRP protein expression (r = 0.45, p<0.05) and BCRP mRNA expression (r = 0.58, p<0.01) in the insulin-managed DM groups. Collectively, the data suggest that the expression of placental efflux transporters is not altered in pregnancies complicated by diabetes when hyperglycemia is managed; however, given the relationship between BCRP expression and plasma hemoglobin A1c levels it is plausible that their expression could change in poorly managed diabetes

Population structure and linkage disequilibrium unravelled in tetraploid potato

Association mapping is considered to be an important alternative strategy for the identification of quantitative trait loci (QTL) as compared to traditional QTL mapping. A necessary prerequisite for association analysis to succeed is detailed information regarding hidden population structure and the extent of linkage disequilibrium. A collection of 430 tetraploid potato cultivars, comprising two association panels, has been analysed with 41 AFLP® and 53 SSR primer combinations yielding 3364 AFLP fragments and 653 microsatellite alleles, respectively. Polymorphism information content values and detected number of alleles for the SSRs studied illustrate that commercial potato germplasm seems to be equally diverse as Latin American landrace material. Genome-wide linkage disequilibrium (LD)—reported for the first time for tetraploid potato—was observed up to approximately 5 cM using r2 higher than 0.1 as a criterion for significant LD. Within-group LD, however, stretched on average twice as far when compared to overall LD. A Bayesian approach, a distance-based hierarchical clustering approach as well as principal coordinate analysis were adopted to enquire into population structure. Groups differing in year of market release and market segment (starch, processing industry and fresh consumption) were repeatedly detected. The observation of LD up to 5 cM is promising because the required marker density is not likely to disable the possibilities for association mapping research in tetraploid potato. Population structure appeared to be weak, but strong enough to demand careful modelling of genetic relationships in subsequent marker-trait association analyses. There seems to be a good chance that linkage-based marker-trait associations can be identified at moderate marker densities