Use of Dried Stoned Olive Pomace in the Feeding of Lactating Buffaloes: Effect on the Quantity and Quality of the Milk Produced

Dried stoned olive pomace (DSOP) was administered to dairy water buffaloes, and their productive performance and milk composition were analysed. Sixteen pluriparous lactating buffaloes were divided into two uniform groups (control and experimental), taking into consideration the following parameters: milk production (2,192 and 2,102 kg) and duration of lactation (254 and 252 d) of the previous year, distance from calving (51 and 43 d), milk production (9.71 and 10.18 kg/d), body condition score (BCS) (6.44 and 6.31) and weight (617 and 653 kg) at the beginning of the trial. Both diets had the same formulation: second cut alfalfa hay 20%, corn silage 42%, concentrate 38% but the two concentrates differed in their formulation, the experimental one contained 15.50% of DSOP as fed. The employed DSOP showed high amounts of secoiridoids, such as 3,4-dihydroxyphenylethanol (3,4-DHPEA) (1.2 g/kg DM), 3,4-dihydroxyphenylethanol-elenolic acid di-aldehyde (3,4-DHPEA-EDA) (12.6 g/kg DM), p-hydroxyphenylethanol-elenolic acid di-aldehyde (p-HPEA-EDA) (5.6 g/kg DM) and lignans, which are known to be powerful bioactive compounds. The control diet had an energy-protein content of 0.86 Milk FU/kg DM and 143.3 g/kg DM of crude protein, whereas the experimental diet of 0.87 Milk FU/kg DM and 146.6 g/kg DM of crude protein. Each animal of the two groups received 17 kg DM/d and each buffalo of the experimental group, by way of the concentrate, ingested 1.05 kg DM/d of DSOP. The trial lasted 40 days. No significant difference was found between the BCS (6.41 and 6.53), live weight (625.93 and 662.50 kg) and milk production (9.69 and 10.08 kg/d) of the two groups, as was the case for fat, protein, lactose, pH and coagulating parameters of the two milks. The milk fat of the experimental group had a significantly higher content of total tocopherols (10.45 vs 8.60 μg/g, p<0.01) and retinol (3.17 vs 2.54 μg/g, p<0.01). The content of the reactive substances with tiobarbituric acid (TBARs) was significantly lower in the milk fat of the experimental group (12.09 vs 15.05 μg MDA/g, p<0.01). The acid content of the milk fat of the experimental group had a significantly higher content (p<0.05) of C18:0 and of C18:3ω6. LC-MS/MS analysis showed the presence of 3,4-DHPEA (36.0 μg/L) in the milk of the DSOP-fed buffaloes, while other phenols were not found. DSOP, in the quantity utilized, can be used in the feeding of the lactating buffalo; the dietetic-nutritional characteristics of the milk are improved due to a greater contribution of tocopherols, retinol and the presence of hydroxytyrosol

Effect of stoned olive pomace on rumen microbial communities and polyunsaturated fatty acids biohydrogenation: an in vitro study.

Background The stoned olive pomace (SOP), which represents approximately 50% of the conversion process of olive in oil, is largely not-utilized and creates costs for its disposal and problems related to environmental impact. In-vitro trial experiments were employed to study the effect of feeds integrated with this bio-waste rich in polyphenols on rumen biohydrogenation, using sheep rumen liquor as inoculum. Results Fatty acid (FA) analysis and polymerase chain reaction denaturing gradient gel electrophoresis (DGGE) approach aimed to microbial community characterization showed that including SOP in feeds at the level of 50 g/kg and 90 g/kg induced changes in FA profile and in microbial populations. The contemporary decrease of Butyrivibrio proteoclasticus and the accumulation of vaccenic acid was observed. A depression of Neisseria weaveri, Ruminobacter amylophilus and other unclassified bacteria related to members of the Lachnospiraceae and Pasteurellaceae families was detected, suggesting that these microbial groups may be involved in rumen biohydrogenation. Conclusions Supplementation of feeds with SOP changes rumen bacterial community, including bacteria responsible for vaccenic acid hydrogenation to stearic acid, thus modifying FA profile of rumen liquor. Hence, an use of SOP aimed to produce meat or dairy products enriched in functional lipids could be hypothesized

ASPETTI DELLA PRODUZIONE DEI PICCOLI RUMINANTI CON IMPATTO SULLA SALUTE UMANA

Negli ultimi anni l’attenzione del consumatore si è sempre più orientata verso le caratteristiche nutrizionali degli alimenti. Queste proprietà sono di grande importanza anche per quanto riguarda le produzioni dei piccoli ruminanti. Il presente lavoro ha lo scopo di riassumere i principali risultati emersi dal progetto di ricerca “Aspetti della produzione dei piccoli ruminanti con particolare impatto sulla salute umana”. Sono stati analizzati mediante i metodi descritti in letteratura: 1) i polimorfismi genetici dei biopeptidi del latte dei piccoli ruminanti; 2) le attività di alcuni enzimi della membrana del globulo di grasso e la frazione lipidica del latte ovino; 3) la qualità nutrizionale del latte e del formaggio ovino in relazione all’intensità di pascolamento; 4) le componenti bioattive di siero e scotta residui alla produzione dei formaggi ovi-caprini; 5) la resistenza genetica alle encefalopatie spongiformi trasmissibili e l’efficienza economica e biologica in razze ovine. I risultati ottenuti evidenziano, da svariati punti di vista, numerose potenzialità legate alle produzioni dei piccoli ruminanti e alle loro ricadute sulla salute umana

Color variations on Victoria quadrangle: support for the geological mapping

Mercury is the closest planet to the Sun. Its extreme thermal environment makes it difficult to explore onsite. In 1974, Mariner 10, the first mission dedicated to Mercury, covered 45% of the surface during of the three Hermean flybys [1]. For about 30 years after Mariner 10, no other mission has flownto Mercury. Many unresolved issues need an answer, and in recent years the interest about Mercury has increased. MESSENGER mission contributed to understand Mercury's origin, its surface structure, and the nature of its magnetic field, exosphere, and magnetosphere [1]. The Mercury Dual Imaging System (MDIS) provided a global coverage of Mercury surface with variable spatial resolution. MDIS is equipped with a narrow angle camera (NAC), dedicated to the study of the geology and a wide angle camera (WAC) with 12 filters useful to investigate the surface composition[2]. Mercury has been divided into 15 quadrangles for mapping purposes [3]. The mapping process permits integration of different geological surface information to better understand the planet crust formation and evolution. Merging spectroscopically data is a poorly followed approach in planetary mapping, but it gives additional information about lithological composition, contributing to the construction of a more complete geological map [e.g. 4]. Recently, [5] proposed a first detailed map of all the Victoria quadrangle (H2). Victoria quadrangle is located in a longitude range between 270°E and 360°E and a latitude range of 22.5°N and 65°N,and itwas only partially mapped by Mariner 10 data[3]. Here we investigate the lithological variation by using the MDIS-WAC data to produce a set of color map products which could be asupport to the geological mapping [5]. The future ESA-JAXA mission to Mercury, BepiColombo, will soon contribute to improve the knowledge of Mercury surface composition and geology thanks to the Spectrometer and Imagers for MPO BepiColombo-Integrated Observatory SYStem (SIMBIO-SYS)[6]

Effetto della somministrazione di selenio e vitamina E sul test di riduzione del NBT in pecora in lattazione

Blood samples were taken from 16 Sardinian ewes on the mean days of 2,7 and 30 of lactation. 8 of 16 animals were treated at the last month of pregnancy with 3 subcutaneous administration of Selenium (0.1 mg/Kg) and Vitamin E (5mg/Kg). The total and differential leukocytes counts and NBT reduction test of leukocytes were determined. Treatment seems to be effective on increase of the percentage of reducent neutrophils

Distinct pathways of antigen processing of MUC1 tumor-associated glycoprotein in dendritic cells (DCs)

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