COVID-19 : do luto e do pesar ao luto prolongado

Copyright © Ordem dos Médicos 2020Aguiar et al made some relevant considerations about funeral rituals and public health measures triggered in Portugal, in the context of the COVID-19 pandemic. One of the issues raised was the mental health support for bereaved family members. This raises a reflection about the particularities of the COVID-19 pandemic, namely the impact on mental health and the experience of mourning for the people affected.info:eu-repo/semantics/publishedVersio

Implementação da técnica Silver In Situ Hibridization para avaliação do status do gene EGFR em doentes com CPNPC

Avaliação do estado do gene Epidermal Growth Factor Receptor (EGFR), por Silver In Situ Hibridization (SISH), tem-se destacado como biomarcador preditivo na resposta à terapêutica. O principal objectivo foi optimizar a etapa de recuperação por calor da metodologia automatizada SISH Dual-Colour, em carcinomas pulmonares fixados em formol durante 24 e 72 horas. A optimização levou a um aumento da preservação do contorno nuclear e da intensidade e contraste dos sinais para os dois tempos de fixação, permitindo avaliar o estado do EGFR em 83,3% dos casos em estudo. A SISH Dual-Colour é uma alternativa para avaliar o estado do EGFR.Assessment of the status of the gene Epidermal Growth Factor Receptor (EGFR), by Silver in Situ Hibridization (SISH) has been highlighted as a predictive biomarker in the response to therapy. The main objective was to optimize the recovery stage of heat-automated methodology SISH Dual-Colour in lung carcinoma with 24 and 72 hours of formaldehyde fixation. The optimization led to an increase in the preservation of the nuclear contour, intensity and contrast of the signals for both fixation times, allowing evaluation of EGFR state in 83.3% of studied cases. The SISH Dual-Colour is an alternative to assess the status of EGFR

Quão preparados estamos para abordar a sexualidade nos cuidados paliativos?

Copyright © Ordem dos Médicos 2019Sexuality, as one of the determinants of quality of life, includes different subjective dimensions related to identity, communication and intimacy. According to the World Health Organization (WHO), sexuality “is a central aspect of being human throughout life and encompasses sex, gender identities and roles, sexual orientation, eroticism, pleasure, intimacy and reproduction.” In this sense, WHO considers intimacy as an important element of the expression of sexuality. This includes individual satisfaction with his/her affective, loving, and sexual relationships. Moreover, in concordance with WHO, and distinct from sexuality and intimacy, sex “refers to the biological characteristics that define humans as female or male”. However, in general use, the term sex is often used to mean ‘sexual activity’.info:eu-repo/semantics/publishedVersio

Japanese management and salary productivity : the case of the electronic and automotive industries in Portugal

Comunicação apresentada nas XX Jornadas Luso-Espanholas de Gestão Científica realizadas de 4-5 de fevereiro 2010 no IPSThe present paper intended to assert in what form the Japanese approach to business management, concerning the factors human resources, research and development and organization and methods, has effect on the value creation by workers, beyond those expressed in salaries. The investigation fell on companies in the electronic and automotive sectors operating in Portugal, with and without Japanese capital, in the six-year span from 2001 to 2006. The specific results demonstrated that that impact is clearly visible over the factor organization and methods. In addition, there are evidences of substantial and direct influence of research and development on the creation of surplus value.info:eu-repo/semantics/publishedVersio

Selectivity of Rynaxypyr for three species of Phytoseiid mites relevant to coffee in Brazil

Among the predaceous mites, those of the Phytoseiidae family are the most important and studied. The phytophagous mites Brevipalpus phoenicis (Geijskes, 1939) (Tenuipalpidae) and Oligonychus ilicis (McGregor, 1917) (Tetranychidae), on coffee trees (Coffea spp.), are frequently found in combination with the predaceous mites Iphiseiodes zuluagai Denmark & Muma, 1972, Amblyseius herbicolus (Chant, 1959) and Euseius citrifolius Denmark & Muma, 1970, among others. The purpose of this research was to study the effects of the insecticide rynaxypyr (chlorantraniliprole 200 SC) on these three species of Phytoseiidae, relevant to coffee and citrus, following standard laboratory procedures. Mated female mites were exposed to fresh-dried residues on a glass surface, with 7 treatments, 5 mites per glass plate and 6 replicates, in a completely randomized experimental design. Each test lasted 8 days, with a daily count of the surviving females and of eggs laid. Rynaxypyr, in all tested concentrations (15, 30, 50, 100 and 500 mg a.i./liter of water), was selective for the studied species, A. herbicolus, I. zuluagai, and E. citrifolius. Overall the treatments resulted in low mortality rates and negligible impact on the reproduction. Therefore, based on IOBC standards, rynaxypyr can be classified as not harmful (Class 1), comparable to the agrochemical hexythiazox equivalent to a harmless standard of selectivity in the laboratory. Rynaxypyr is therefore a complement to programs of integrated pest management, to preserve the populations of predatory mites in crops of coffee and citrus, among others, in Brazil.Among the predaceous mites, those of the Phytoseiidae family are the most important and studied. The phytophagous mites Brevipalpus phoenicis (Geijskes, 1939) (Tenuipalpidae) and Oligonychus ilicis (McGregor, 1917) (Tetranychidae), on coffee trees (Coffea spp.), are frequently found in combination with the predaceous mitesIphiseiodes zuluagai Denmark & Muma, 1972, Amblyseius herbicolus (Chant, 1959) and Euseius citrifolius Denmark & Muma, 1970, among others. The purpose of this research was to study the effects of the insecticide rynaxypyr (chlorantraniliprole 200 SC) on these three species of Phytoseiidae, relevant to coffee and citrus, following standard laboratory procedures. Mated female mites were exposed to fresh-dried residues on a glass surface, with 7 treatments, 5 mites per glass plate and 6 replicates, in a completely randomized experimental design. Each test lasted 8 days, with a daily count of the surviving females and of eggs laid. Rynaxypyr, in all tested concentrations (15, 30, 50, 100 and 500 mg a.i./liter of water), was selective for the studied species, A. herbicolus, I. zuluagai, and E. citrifolius. Overall the treatments resulted in low mortality rates and negligible impact on the reproduction. Therefore, based on IOBC standards, rynaxypyr can be classified as not harmful (Class 1), comparable to the agrochemical hexythiazox equivalent to a harmless standard of selectivity in the laboratory. Rynaxypyr is therefore a complement to programs of integrated pest management, to preserve the populations of predatory mites in crops of coffee and citrus, among others, in Brazil

Ultrasound-assisted biosynthesis of novel methotrexate-conjugates

New methotrexate-acylglycerols and methotrexate-cyclodextrins (, and -CD) conjugates were obtained via esterification or transesterification reactions. All reactions were catalysed by esterases namely immobilized Lipase from Candida antarctica B and Lipase from Thermomyces lanuginosus. The use of ultrasound to assist the reactions revealed to be a key factor to obtain high conversion yields on both MTX conjugates. Transesterification reactions including long chain triacylglycerols were only successful when ultrasound was applied. In cyclodextrins esterification a higher number of MTX molecules was also linked to cyclodextrins when ultrasound was used. All the conjugates were characterized by MALDI-TOF and NMR spectroscopy.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684) and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by European Regional Development Fund under the scope of Norte2020 – Programa Operacional Regional do Norte. Jennifer Noro also thanks to FCT – Fundação para a Ciência e a Tecnologia for funding her scholarship (SFRH/BD/121673/2016). This work has also received funding from the European Union Horizon 2020 research and innovation programme under grant agreement NMP-06-2015-683356 FOLSMART.info:eu-repo/semantics/publishedVersio

LC-HRMS profiling of paralytic shellfish toxins in Mytilus galloprovincialis after a Gymnodinium catenatum bloom

Saxitoxin and its more than 50 analogues are a group of naturally occurring neurotoxins collectively designated as paralytic shellfish toxins (PSTs). PSTs are toxic to humans and maximum legal limits in seafood have been implemented by regulatory authorities worldwide. In the European Union, monitoring of PSTs is performed using the AOAC Official Method 2005.06, based on liquid chromatography coupled with fluorescence detection (LC- FLD). However, this method has been suggested to not effectively detect the emerging C-11 hydroxyl (M-toxins) and benzoate (GC-toxins) analogues, with these analogues currently not being surveyed in monitoring programs. In this study, a liquid chromatography-high resolution mass spectrometry (LC-HRMS) method was used to search for these emerging PSTs in mussels, Mytilus galloprovincialis, contaminated following an intense Gymnodinium catenatum bloom in the Tagus estuary (Lisbon, Portugal). Five M-toxins (M1, M2, M6, dcM6, and dcM10), but no GC-toxins, were detected in the mussels’ whole-soft body tissue. Moreover, the classical PSTs (C1 to C4, GTX 4 to GTX6, dcGTX1 to dcGTX4, dcSTX, dcNEO, and STX) were also found and comprised the largest fraction of the PSTs’ profile. The presence of unregulated PSTs in edible mussel samples suggests potential seafood safety risks and urges further research to determine the frequency of these analogues in seafood and their contribution to toxicityinfo:eu-repo/semantics/publishedVersio