Evidenciação de antígeno da hepatite B (HBsAg) em "manchas" obtidas de exemplares de Triatominae: nota prévia

A total of 890 impressions on filter paper identified as containing human blood, were examined for the presence of hepatitis B antigen (HBsAg), using the Hepanosticon haemagglutination and radioimmunoassay techniques. The samples examined belong to the Panstrongylus, Rhodnius and Triatoma genres. The Hepanosticon haemagglutination technique showed that 7 impressions, after elution, were presumably positive for HBsAg, but by radioimmunoassay, this antigen could only be detected in 1. The positive sample was obtained from a specimen of Triatoma sordida (fifth instar nymph).De 890 "manchas" provenientes de insetos dos gêneros Panstrongylus, Rhodnius e Triatoma, positivas para sangue humano, 7 deram resultados presumptivamente positivos por hemaglutinação passiva para HBsAg. Deste total só um caso foi confirmado como positivo, por RIE (radioimunoensaio), correspondendo a uma "mancha" obtida de Triatoma sordida (ninfa de 5.º estádio)

Estudo da evolução da fertilidade do solo em pomares de pessegueiros - situação inicial

A região da Beira Interior é a principal região de produção de pêssegos do país. Esta cultura, pelo carácter plurianual, pode ter uma ação positiva ou negativa na melhoria do teor em matéria orgânica e da fertilidade do solo, de acordo com as técnicas utilizadas na manutenção do solo e gestão da sua fertilidade. No âmbito da manutenção do solo, a técnica de enrelvamento da entrelinha e a técnica de destroçamento da lenha de poda, são consideradas como positivas no aumento do teor em matéria orgânica do solo o que se reflete na melhoria da sua fertilidade. Contudo, esse efeito será sempre a longo prazo, não estando disponível informação sobre a evolução dos parâmetros de fertilidade do solo em pomares de pessegueiros, para as condições de Portugal em geral e especificamente para a Beira Interior. Para monitorizar o efeito da gestão inerente a um pomar nas características de fertilidade do solo foram estabelecidas unidades de observação em 26 pomares de pessegueiros instalados na Beira Interior entre janeiro e março de 2018. Cada unidade de observação, correspondente a um pomar, compreende a marcação de 10 plantas, distribuídas em três blocos, num total de 20 pontos de amostragem de terra, sendo 10 pontos na linha de plantação e 10 pontos correspondentes à entrelinha. Todos os pontos de amostragem foram georreferenciados. Os resultados referentes à fertilidade do solo dos pomares de pessegueiros após a instalação indicam uma média global do teor de matéria orgânica de 2,8% não havendo diferenças significativas entre linha e entrelinha. A média do pH foi de 6,3 na linha e 6,1 na entrelinha. A média do teor de K2O foi de 404 mg.kg-1 na linha e 242 mg.kg-1 na entrelinha e a média do teor de P2O5 mg.kg-1 foi de 201 mg.kg-1 na linha e 114 mg.kg-1 na entrelinha, indicando uma prática de fertilização de fundo localizada na linha de plantação. Estes resultados correspondem à situação inicial dos pomares, prevendo-se a recolha de amostras no mesmo local com uma periodicidade de 4 anos, de modo a realizar a monitorização da fertilidade do solo ao longo da vida útil do pomar, minimizando a variabilidade inerente à heterogeneidade dos solos uma vez que os pontos de recolha estão georreferenciados.info:eu-repo/semantics/publishedVersio

Predictors of Psychological Well-Being during Behavioral Obesity Treatment in Women

This study examined the association of autonomy-related variables, including exercise motivation, with psychological well-being and quality of life, during obesity treatment. Middle-aged overweight/obese women (n = 239) participated in a 1-year behavioral program and completed questionnaires measuring need support, general self-determination, and exercise and treatment motivation. General and obesity-specific health-related quality of life (HRQOL), self-esteem, depression, and anxiety were also assessed. Results showed positive correlations of self-determination and perceived need support with HRQOL and self-esteem, and negative associations with depression and anxiety (P < .001). Treatment autonomous motivation correlated positively with physical (P = .004) and weight-related HRQOL (P < .001), and negatively with depression (P = .025) and anxiety (P = .001). Exercise autonomous motivation was positively correlated with physical HRQOL (P < .001), mental HRQOL (P = .003), weight-related HRQOL (P < .001), and self-esteem (P = .003), and negatively with anxiety (P = .016). Findings confirm that self-determination theory's predictions apply to this population and setting, showing that self-determination, perceived need support, and autonomous self-regulation positively predict HRQOL and psychological well-being

Bioaccumulation of amylose-like glycans by Helicobacter pylori

Background: Helicobacter pylori cell surface is composed of lipopolysaccharides (LPSs) yielding structures homologous to mammalian Lewis O-chains blood group antigens. These structures are key mediators in the definition of host-microbial interactions and known to change their expression pattern in response to environmental pressure. Aims:  The present work is focused on the identification of new H. pylori cell-surface glycosides. Special attention is further devoted to provide insights on the impact of in vitro subcultivation on H. pylori cell-surface phenotypes. Methods:  Cell-surface glycans from H. pylori NCTC 11637 and two clinical isolates were recovered from the aqueous phase resulting from phenol:water extraction of intact bacteria. They were evaluated in relation to their sugars and glycosidic-linkages composition by CG-MS, size-exclusion chromatography, NMR, and Mass Spectrometry. H. pylori glycan profile was also monitored during subcultivation in vitro in agar and F12 liquid medium. Results:  All three studied strains produce LPS expressing Lewis epitopes and express bioaccumulate amylose-like glycans. Bioaccumulation of amylose was found to be enhanced with the subcultivation of the bacterium on agar medium and accompanied by a decrease in the expression of LPS O-chains. In contrast, during exponential growth in F12 liquid medium, an opposite behavior is observed, that is, there is an increase in the overall amount of LPS and decrease in amylose content. Conclusions:  This work shows that under specific environmental conditions, H. pylori expresses a phase-variable cell-surface α-(1→4)-glucose moiety

New cathepsin V inhibitor from stems of Bowdichia virgilioides

Bioassay-guided fractionation of Bowdichia virgilioides Kunth, Fabaceae, extracts has led to the isolation of cathepsin V inhibitors. The investigation of the hexane and ethyl acetate extracts allowed the characterization of eleven compounds: lupeol, lupenone, β-sitosterol and stigmasterol in mixture, trans p-coumaric acid ester derivative, syringaresinol, bowdenol, 8-methoxycoumestrol, 3,4-hydroxy-7-methoxyisoflavone, 7,3′-dihydroxy-4′-methoxyisoflavone, and 5,4′-dihydroxy-7′-methoxyisoflavone. Structures of compounds were stablished by 1D and 2D NMR, and MS experiments. Among the isolated compounds, trans p-coumaric acid ester derivative and 8-methoxycoumestrol showed significant inhibition on cathepsin V, which is up to now unexplored. Keywords: Cathepsin, Cysteine peptidases, Inhibitors, Cerrado biom

Comparison of PBO solvers in a dependency solving domain

Linux package managers have to deal with dependencies and conflicts of packages required to be installed by the user. As an NP-complete problem, this is a hard task to solve. In this context, several approaches have been pursued. Apt-pbo is a package manager based on the apt project that encodes the dependency solving problem as a pseudo-Boolean optimization (PBO) problem. This paper compares different PBO solvers and their effectiveness on solving the dependency solving problem.Comment: In Proceedings LoCoCo 2010, arXiv:1007.083

Inspiratory Muscle Training Improves Blood Flow to Resting and Exercising Limbs in Patients With Chronic Heart Failure

ObjectivesWe tested the hypothesis that inspiratory muscle loading could result in exaggerated peripheral vasoconstriction in resting and exercising limbs and that inspiratory muscle training (IMT) could attenuate this effect in patients with chronic heart failure (CHF) and inspiratory muscle weakness.BackgroundInspiratory muscle training improves functional capacity of patients with CHF, but the mechanisms of this effect are unknown.MethodsEighteen patients with CHF and inspiratory muscle weakness (maximal inspiratory pressure <70% of predicted) and 10 healthy volunteers participated in the study. Inspiratory muscle loading was induced by the addition of inspiratory resistance of 60% of maximal inspiratory pressure, while blood flow to the resting calf (CBF) and exercising forearm (FBF) were measured by venous occlusion plethysmography. For the patients with CHF, blood flow measurements as well as ultrasound determination of diaphragm thickness were made before and after a 4-week program of IMT.ResultsWith inspiratory muscle loading, CHF patients demonstrated a more marked reduction in resting CBF and showed an attenuated rise in exercising FBF when compared with control subjects. After 4 weeks of IMT, CHF patients presented hypertrophy of the diaphragm and improved resting CBF and exercise FBF with inspiratory muscle loading.ConclusionsIn patients with CHF and inspiratory muscle weakness, inspiratory muscle loading results in marked reduction of blood flow to resting and exercising limbs. Inspiratory muscle training improves limb blood flow under inspiratory loading in these patients