Spirituality and counseling : an integration into practice

Mental health counselors have traditionally practiced the idea of separation, not integration, in regards to the exploration and use of spirituality in the counseling setting. Research and shifting trends have shown spirituality is not only important to clientele, but these clients also express a desire to incorporate their spirituality into counseling. Through enforcing appropriate competencies and guidelines, incorporating spirituality has proven effective within clinical settings. Counselors who do desire to integrate spirituality into counseling have limited resources available for guidance. The purpose of this paper is three fold, to provide rationale for the integration of spirituality in counseling, discuss important considerations when integrating spirituality, and provide brief technique examples of integrating spirituality in counseling

Trans Discrimination in Europe. A TGEU analysis of the FRA LGBTI Survey 2019

The present report presents selected data from the LGBTI Survey 2019 conducted by the European Union Agency for Fundamental Rights (FRA) with a focus on the 20.933 trans respondents. This is the largest survey on trans people in Europe.This report discusses the socio-demographic situation, life satisfaction, level of openness, experiences with trans-specific legal and medical procedures, and exposure to discrimination, violence, and harassment. It aims at reducing the gap regarding available data on the lived experiences of trans people in Europe

FluoroSpot Analysis of TLR-Activated Monocytes Reveals Several Distinct Cytokine-Secreting Subpopulations

Monocytes have long been considered a heterogeneous group of cells both in terms of morphology and function. In humans, three distinct subsets have been described based on their differential expression of the cell surface markers CD14 and CD16. However, the relationship between these subsets and the production of cytokines has for the most part been based on ELISA measurements, making it difficult to draw conclusions as to their functional profile on the cellular level. In this study, we have investigated lipoteichoic acid (LTA)- and lipopolysaccharide (LPS)-induced cytokine secretion by monocytes using the FluoroSpot technique. This method measures the number of cytokine-secreting cells on the single-cell level and uses fluorescent detection, allowing for the simultaneous analysis of two cytokines from the same population of isolated cells. By this approach, human monocytes from healthy volunteers could be divided into several subgroups as IL-1β, IL-6, TNF-α and MIP-1β were secreted by larger populations of responding cells (25.9–39.2%) compared with the smaller populations of GM-CSF (9.1%), IL-10 (1.3%) and IL-12p40 (1.2%). Furthermore, when studying co-secretion in FluoroSpot, an intricate relationship between the monocytes secreting IL-1β and/or IL-6 and those secreting TNF-α, MIP-1β, GM-CSF, IL-10 and IL-12p40 was revealed. In this way, dissecting the secretion pattern of the monocytes in response to TLR-2 or TLR-4 stimulation, several subpopulations with distinct cytokine-secreting profiles could be identified