Cell cycle regulation of a Xenopus Wee1-like kinase

Using a polymerase chain reaction-based strategy, we have isolated a gene encoding a Wee1-like kinase from Xenopus eggs. The recombinant Xenopus Wee1 protein efficiently phosphorylates Cdc2 exclusively on Tyr- 15 in a cyclin-dependent manner. The addition of exogenous Wee1 protein to Xenopus cell cycle extracts results in a dose-dependent delay of mitotic initiation that is accompanied by enhanced tyrosine phosphorylation of Cdc2. The activity of the Wee1 protein is highly regulated during the cell cycle: the interphase, underphosphorylated form of Wee1 (68 kDa) phosphorylates Cdc2 very efficiently, whereas the mitotic, hyperphosphorylated version (75 kDa) is weakly active as a Cdc2-specific tyrosine kinase. The down-modulation of Wee1 at mitosis is directly attributable to phosphorylation, since dephosphorylation with protein phosphatase 2A restores its kinase activity. During interphase, the activity of this Wee1 homolog does not vary in response to the presence of unreplicated DNA. The mitosis-specific phosphorylation of Wee1 is due to at least two distinct kinases: the Cdc2 protein and another activity (kinase X) that may correspond to an MPM-2 epitope kinase. These studies indicate that the down-regulation of Wee1-like kinase activity at mitosis is a multistep process that occurs after other biochemical reactions have signaled the successful completion of S phase

Pupal surveys for Aedes aegypti surveillance and potential targeted control in residential areas of Mérida, México.

A mosquito larval-pupal survey was conducted in 1,160 households of the Mexican city of Mérida during the rainy season of 2003 to determine their differential productivity for Aedes aegypti. Larvae and pupae were detected in 15 broad categories of container types. All breeding sites were found in the patios (backyards) and were potentially rain filled. Ae. aegypti pupae were produced from all categories of breeding site, and no single container type was predominately responsible for pupal production. The most productive buckets comprised 42% of the pupae-positive containers and provided 34% of the total pupae collected. Pupal production in buckets, together with plastic rubbish, pet dishes and basins, utensils for cooking and washing, tires, and flowerpots, accounted for almost 87% of pupal production. However, the most important pupal producers had low infestation indices for immature forms, illustrating that the use of positive-container indices can underestimate the importance of certain breeding sites. Overall, 40% of containers that were observed harboring Ae. aegypti pupae were classified as disposable. The remaining containers were considered useful, although some were seldom used. The discussion focuses on the potential utility of the pupal survey for targeting control, and its resulting pupae-per-person entomological indicator, both for comparison with a theoretical threshold for dengue transmission and for targeting vector control in this Mexican city

The association between treatment adherence to nicotine patches and smoking cessation in pregnancy

Background: In non-pregnant “quitters,” adherence to nicotine replacement therapy (NRT) increases smoking cessation. We investigated relationships between adherence to placebo or NRT patches and cessation in pregnancy, including an assessment of reverse causation and whether any adherence: cessation relationship is moderated when using nicotine or placebo patches. Methods: Using data from 1050 pregnant trial participants, regression models investigated associations between maternal characteristics, adherence and smoking cessation. Results: Adherence during the first month was associated with lower baseline cotinine concentrations (β −0.08, 95% confidence interval [CI] −0.15 to −0.01) and randomization to NRT (β 2.59, 95%CI 1.50 to 3.68). Adherence during both treatment months was associated with being randomized to NRT (β 0.51, 95% CI 0.29 to 0.72) and inversely associated with higher nicotine dependence. Adherence with either NRT or placebo was associated with cessation at 1 month (odds ratio [OR] 1.11, 95% CI 1.08 to 1.13) and delivery (OR 1.06, 95% CI 1.03 to 1.09), but no such association was observed in the subgroup where reverse causation was not possible. Amongst all women, greater adherence to nicotine patches was associated with increased cessation (OR 2.47, 95% CI 1.32 to 4.63) but greater adherence to placebo was not (OR 0.98, 95% CI: 0.44 to 2.18). Conclusion: Women who were more adherent to NRT were more likely to achieve abstinence; more nicotine dependent women probably showed lower adherence to NRT because they relapsed to smoking more quickly. The interaction between nicotine-containing patches and adherence for cessation suggests that the association between adherence with nicotine patches and cessation may be partly causal

Sixteen years of Collaborative Learning through Active Sense-making in Physics (CLASP) at UC Davis

This paper describes our large reformed introductory physics course at UC Davis, which bioscience students have been taking since 1996. The central feature of this course is a focus on sense-making by the students during the five hours per week discussion/labs in which the students take part in activities emphasizing peer-peer discussions, argumentation, and presentations of ideas. The course differs in many fundamental ways from traditionally taught introductory physics courses. After discussing the unique features of CLASP and its implementation at UC Davis, various student outcome measures are presented showing increased performance by students who took the CLASP course compared to students who took a traditionally taught introductory physics course. Measures we use include upper-division GPAs, MCAT scores, FCI gains, and MPEX-II scores.Comment: Also submitted to American Journal of Physic

Self-dual Maxwell Chern-Simons Solitons In 1+1 Dimensions

We study the domain wall soliton solutions in the relativistic self-dual Maxwell Chern-Simons model in 1+1 dimensions obtained by the dimensional reduction of the 2+1 model. Both topological and nontopological self-dual solutions are found in this case. A la BPS dyons here the Bogomol'ny bound on the energy is expressed in terms of two conserved quantities. We discuss the underlying supersymmetry. Nonrelativistic limit of this model is also considered and static, nonrelativistic self-dual soliton solutions are obtained.Comment: 18 pages RevTex, 2 figures included, to appear in Phys. Rev.

Controlling malaria using livestock-based interventions: a one health approach.

Where malaria is transmitted by zoophilic vectors, two types of malaria control strategies have been proposed based on animals: using livestock to divert vector biting from people (zooprophylaxis) or as baits to attract vectors to insecticide sources (insecticide-treated livestock). Opposing findings have been obtained on malaria zooprophylaxis, and despite the success of an insecticide-treated livestock trial in Pakistan, where malaria vectors are highly zoophilic, its effectiveness is yet to be formally tested in Africa where vectors are more anthropophilic. This study aims to clarify the different effects of livestock on malaria and to understand under what circumstances livestock-based interventions could play a role in malaria control programmes. This was explored by developing a mathematical model and combining it with data from Pakistan and Ethiopia. Consistent with previous work, a zooprophylactic effect of untreated livestock is predicted in two situations: if vector population density does not increase with livestock introduction, or if livestock numbers and availability to vectors are sufficiently high such that the increase in vector density is counteracted by the diversion of bites from humans to animals. Although, as expected, insecticide-treatment of livestock is predicted to be more beneficial in settings with highly zoophilic vectors, like South Asia, we find that the intervention could also considerably decrease malaria transmission in regions with more anthropophilic vectors, like Anopheles arabiensis in Africa, under specific circumstances: high treatment coverage of the livestock population, using a product with stronger or longer lasting insecticidal effect than in the Pakistan trial, and with small (ideally null) repellency effect, or if increasing the attractiveness of treated livestock to malaria vectors. The results suggest these are the most appropriate conditions for field testing insecticide-treated livestock in an Africa region with moderately zoophilic vectors, where this intervention could contribute to the integrated control of malaria and livestock diseases

Self-dual Vortices in the Generalized Abelian Higgs Model with Independent Chern-Simons Interaction

Self-dual vortex solutions are studied in detail in the generalized abelian Higgs model with independent Chern-Simons interaction. For special choices of couplings, it reduces to a Maxwell-Higgs model with two scalar fields, a Chern-Simons-Higgs model with two scalar fields, or other new models. We investigate the properties of the static solutions and perform detailed numerical analyses. For the Chern-Simons-Higgs model with two scalar fields in an asymmetric phase, we prove the existence of multisoliton solutions which can be viewed as hybrids of Chern-Simons vortices and $CP^1$ lumps. We also discuss solutions in a symmetric phase with the help of the corresponding exact solutions in its nonrelativistic limit. The model interpolating all three models---Maxwell-Higgs, Chern-Simons-Higgs, and $CP^1$ models--- is discussed briefly. Finally we study the possibility of vortex solutions with half-integer vorticity in the special case of the model. Numerical results are negative.Comment: 32 pages, LATEX, SNUTP 92-7

Conformal topological Yang-Mills theory and de Sitter holography

A new topological conformal field theory in four Euclidean dimensions is constructed from N=4 super Yang-Mills theory by twisting the whole of the conformal group with the whole of the R-symmetry group, resulting in a theory that is conformally invariant and has two conformally invariant BRST operators. A curved space generalisation is found on any Riemannian 4-fold. This formulation has local Weyl invariance and two Weyl-invariant BRST symmetries, with an action and energy-momentum tensor that are BRST-exact. This theory is expected to have a holographic dual in 5-dimensional de Sitter space.Comment: 34 pages, AMSTex, Reference adde

Massless Scalar QED with Non-minimal Chern Simons Coupling

2+1 dimensional massless scalar QED with $(\phi^*\phi)^3$ scalar self-coupling is modified by the addition of a non- minimal Chern-Simons term that couples the dual of the electromagnetic field strength to the covariant current of the complex scalar field. The theory is shown to be fully one- loop renormalizable. The one loop effective potential for the scalar field gives rise to spontaneous symmetry breaking which induces masses for both the scalar and vector fields. At high temperature there is a symmetry restoring phase transition.Comment: 18 pages, latex, preprint WIN-93-1