Worldwide Husbanding Process Improvement: Comparative Analysis of Contracting Methodologies

Sponsored Report (for Acquisition Research Program)This study is designed to support one of three major focus areas in the Naval Supply Systems Command (NAVSUP) Worldwide Husbanding Improvement Process initiative. Existing contracting methodologies were analyzed using the following methods: characteristics of existing contract vehicles within forecasting and simulation frameworks; strengths, weaknesses, opportunities, threats (SWOT) analysis, and stakeholder analysis. Conclusions are drawn and recommendations are outlined for optimum methods of contracting for husbanding services as requested by the Worldwide Husbanding Process Improvement Action Team. Historical husbanding contract data were reviewed, including constraints and desired performance criteria. Implementation of a flat-rate, low-variability, well-defined and constant set of requirements minimizes risk and price fluctuations. Conversely, adoption of a cost-reimbursable contract type is both undesirable and infeasible. A contracting methodology that represents a best-value trade-off within constraints should be flexible and risk-based while offering performance-based incentives.Naval Postgraduate School Acquisition Research ProgramApproved for public release; distribution is unlimited

A genome scan for quantitative trait loci affecting the Salmonella carrier-state in the chicken

Selection for increased resistance to Salmonella colonisation and excretion could reduce the risk of foodborne Salmonella infection. In order to identify potential loci affecting resistance, differences in resistance were identified between the N and 61 inbred lines and two QTL research performed. In an F2 cross, the animals were inoculated at one week of age with Salmonella enteritidis and cloacal swabs were carried out 4 and 5 wk post inoculation (thereafter called CSW4F2 and CSW4F2) and caecal contamination (CAECF2) was assessed 1 week later. The animals from the (N × 61) × N backcross were inoculated at six weeks of age with Salmonella typhimurium and cloacal swabs were studied from wk 1 to 4 (thereafter called CSW1BC to CSW4BC). A total of 33 F2 and 46 backcross progeny were selectively genotyped for 103 and 135 microsatellite markers respectively. The analysis used least-squares-based and non-parametric interval mapping. Two genome-wise significant QTL were observed on Chromosome 1 for CSW2BC and on Chromosome 2 for CSW4F2, and four suggestive QTL for CSW5F2 on Chromosome 2, for CSW5F2 and CSW2BC on chromosome 5 and for CAECF2 on chromosome 16. These results suggest new regions of interest and the putative role of SAL1

Microsatellite mapping of QTLs affecting resistance to coccidiosis (Eimeria tenella) in a Fayoumi × White Leghorn cross

<p>Abstract</p> <p>Background</p> <p>Avian coccidiosis is a major parasitic disease of poultry, causing severe economical loss to poultry production by affecting growth and feed efficiency of infected birds. Current control strategies using mainly drugs and more recently vaccination are showing drawbacks and alternative strategies are needed. Using genetic resistance that would limit the negative and very costly effects of the disease would be highly relevant. The purpose of this work was to detect for the first time QTL for disease resistance traits to <it>Eimeria tenella </it>in chicken by performing a genome scan in an F2 cross issued from a resistant Fayoumi line and a susceptible Leghorn line.</p> <p>Results</p> <p>The QTL analysis detected 21 chromosome-wide significant QTL for the different traits related to disease resistance (body weight growth, plasma coloration, hematocrit, rectal temperature and lesion) on 6 chromosomes. Out of these, a genome-wide very significant QTL for body weight growth was found on GGA1, five genome-wide significant QTL for body weight growth, plasma coloration and hematocrit and one for plasma coloration were found on GGA1 and GGA6, respectively. Two genome-wide suggestive QTL for plasma coloration and rectal temperature were found on GGA1 and GGA2, respectively. Other chromosme-wide significant QTL were identified on GGA2, GGA3, GGA6, GGA15 and GGA23. Parent-of-origin effects were found for QTL for body weight growth and plasma coloration on GGA1 and GGA3. Several QTL for different resistance phenotypes were identified as co-localized on the same location.</p> <p>Conclusion</p> <p>Using an F2 cross from resistant and susceptible chicken lines proved to be a successful strategy to identify QTL for different resistance traits to <it>Eimeria tenella</it>, opening the way for further gene identification and underlying mechanisms and hopefully possibilities for new breeding strategies for resistance to coccidiosis in the chicken. From the QTL regions identified, several candidate genes and relevant pathways linked to innate immune and inflammatory responses were suggested. These results will be combined with functional genomics approaches on the same lines to provide positional candidate genes for resistance loci for coccidiosis. Results suggested also for further analysis, models tackling the complexity of the genetic architecture of these correlated disease resistance traits including potential epistatic effects.</p

[Avian cytogenetics goes functional] Third report on chicken genes and chromosomes 2015

High-density gridded libraries of large-insert clones using bacterial artificial chromosome (BAC) and other vectors are essential tools for genetic and genomic research in chicken and other avian species... Taken together, these studies demonstrate that applications of large-insert clones and BAC libraries derived from birds are, and will continue to be, effective tools to aid high-throughput and state-of-the-art genomic efforts and the important biological insight that arises from them

Worldwid husbanding process improvement: comparative analysis of contractig methodologies

Acquisition research (Graduate School of Business & Public Policy)This study is designed to support one of three major focus areas in the Naval Supply Systems Command (NAVSUP) Worldwide Husbanding Improvement Process initiative. Existing contracting methodologies were analyzed using the following methods: characteristics of existing contract vehicles within forecasting and simulation frameworks; strengths, weaknesses, opportunities, threats (SWOT) analysis, and stakeholder analysis. Conclusions are drawn and recommendations are outlined for optimum methods of contracting for husbanding services as requested by the Worldwide Husbanding Process Improvement Action Team. Historical husbanding contract data were reviewed, including constraints and desired performance criteria. Implementation of a flat-rate, low-variability, well-defined and constant set of requirements minimizes risk and price fluctuations. Conversely, adoption of a cost-reimbursable contract type is both undesirable and infeasible. A contracting methodology that represents a best-value trade-off within constraints should be flexible and risk-based while offering performance-based incentives.Approved for public release; distribution is unlimited

Worldwide husbanding process improvement: comparative analysis of multiple contracting methodologies

MBA Professional ReportThis study is designed to support one of three major focus areas in the Naval Supply Systems Command (NAVSUP) Worldwide Husbanding Improvement Process initiative. Existing contracting methodologies were analyzed using the following methods: characteristics of existing contract vehicles within forecasting and simulation frameworks; strengths, weaknesses, opportunities, threats (SWOT) analysis, and stakeholder analysis. Conclusions are drawn and recommendations are outlined for optimum methods of contracting for husbanding services as requested by the Worldwide Husbanding Process Improvement Action Team. Historical husbanding contract data were reviewed, including constraints and desired performance criteria. Implementation of a flat-rate, low-variability, well-defined and constant set of requirements minimizes risk and price fluctuations. Conversely, adoption of a cost-reimbursable contract type is both undesirable and infeasible. A contracting methodology that represents a best-value trade-off within constraints should be flexible and risk-based while offering performance-based incentives.http://archive.org/details/worldwidehusband1094510246Approved for public release; distribution is unlimited