Water-soaked porous evidence:a comparison of processing methods

This study compared the U.K. Home Office formulation for physical developer (PD) against Oil Red O (ORO) and a modified formulation of physical developer (MPD) that uses Tween 20 instead of Synperonic-N for enhancing fingermarks. Three different donors deposited fingermarks on porous surfaces (white paper, leaflets, and cardboard), with aging periods varying from 7 to 28 days. None of the techniques that were tested provided enhancement of latent fingermarks on leaflets, whereas poor-quality enhancement was observed on cardboard. In contrast, all techniques were more successful on white paper surfaces. The results obtained on white paper suggested that PD and MPD performed similarly, with PD detecting 82.3% of the deposited fingermarks and MPD detecting 86.5% of the deposited fingermarks. PD yielded a higher percentage (38.5%) of fingermarks with fine ridge detail (i.e., those with grade 2 or above) than MPD (35.4%). ORO, however, yielded poor results, enhancing only 4.5% of latent fingermarks, but showed no ridge detail in any of the enhancements (i.e., only showed grade 1 enhancements.

G-CSF increases mesenchymal precursor cell numbers in the bone marrow via an indirect mechanism involving osteoclast-mediated bone resorption

AbstractDuring the course of studies to investigate whether MPC circulate in response to G-CSF, the agent most frequently used to induce mobilization of hematopoietic progenitors, we observed that while G-CSF failed to increase the number of MPC in circulation (assayed in vitro as fibroblast colony-forming cells, CFU-F), G-CSF administration nevertheless resulted in a time-dependent increase in the absolute number of CFU-F within the BM, peaking at Day 7. Treatment of BM cells from G-CSF-treated mice with hydroxyurea did not alter CFU-F numbers, suggesting that the increase in their numbers in response to G-CSF administration is not due to proliferation of existing CFU-F. Given previous studies demonstrating that G-CSF potently induces bone turnover in mice, we hypothesized that the increase in CFU-F may be triggered by the bone resorption that occurs following G-CSF administration. In accord with this hypothesis, administration of an inhibitor of osteoclast differentiation, osteoprotegerin (OPG), prevented the increase of CFU-F numbers induced by G-CSF. In conclusion, these data indicate that the cytokine treatment routinely used to mobilize hematopoietic stem cells could provide a readily applicable method to induce in vivo expansion of MPC for clinical applications

Prospectus, November 15, 2000

https://spark.parkland.edu/prospectus_2000/1031/thumbnail.jp

Screening for type 2 diabetes is feasible, acceptable, but associated with increased short-term anxiety: a randomised controlled trial in British general practice.

BACKGROUND: To assess the feasibility and uptake of a diabetes screening programme; to examine the effects of invitation to diabetes screening on anxiety, self-rated health and illness perceptions. METHODS: Randomised controlled trial in two general practices in Cambridgeshire. Individuals aged 40-69 without known diabetes were identified as being at high risk of having undiagnosed type 2 diabetes using patient records and a validated risk score (n = 1,280). 355 individuals were randomised in a 2 to 1 ratio into non-invited (n = 238) and invited (n = 116) groups. A stepwise screening programme confirmed the presence or absence of diabetes. Six weeks after the last contact (either test or invitation), a questionnaire was sent to all participants, including non-attenders and those who were not originally invited. Outcome measures included attendance, anxiety (short-form Spielberger State Anxiety Inventory-STAI), self-rated health and diabetes illness perceptions. RESULTS: 95 people (82% of those invited) attended for the initial capillary blood test. Six individuals were diagnosed with diabetes. Invited participants were more anxious than those not invited (37.6 vs. 34.1 STAI, p-value = 0.015), and those diagnosed with diabetes were considerably more anxious than those classified free of diabetes (46.7 vs. 37.0 STAI, p-value = 0.031). Non-attenders had a higher mean treatment control sub-scale (3.87 vs. 3.56, p-value = 0.016) and a lower mean emotional representation sub-scale (1.81 vs. 2.68, p-value = 0.001) than attenders. No differences in the other five illness perception sub-scales or self-rated health were found. CONCLUSION: Screening for type 2 diabetes in primary care is feasible but may be associated with higher levels of short-term anxiety among invited compared with non-invited participants.RIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are

The dynamic genetic repertoire of microbial communities

Community genomic data have revealed multiple levels of variation between and within microbial consortia. This variation includes large-scale differences in gene content between ecosystems as well as within-population sequence heterogeneity. In the present review, we focus specifically on how fine-scale variation within microbial and viral populations is apparent from community genomic data. A major unresolved question is how much of the observed variation is due to neutral vs. adaptive processes. Limited experimental data hint that some of this fine-scale variation may be in part functionally relevant, whereas sequence-based and modeling analyses suggest that much of it may be neutral. While methods for interpreting population genomic data are still in their infancy, we discuss current interpretations of existing datasets in the light of evolutionary processes and models. Finally, we highlight the importance of virus–host dynamics in generating and shaping within-population diversity

Biochemical and clinical response after umbilical cord blood transplant in a boy with early childhood-onset beta-mannosidosis.

BACKGROUND: Deficiency in the enzyme β-mannosidase was described over three decades ago. Although rare in occurrence, the presentation of childhood-onset β-mannosidase deficiency consists of hypotonia in the newborn period followed by global development delay, behavior problems, and intellectual disability. No effective pharmacologic treatments have been available. METHODS: We report 2-year outcomes following the first umbilical cord blood transplant in a 4-year-old boy with early childhood-onset disease. RESULTS: We show restoration of leukocyte β-mannosidase activity which remained normal at 2 years posttransplant, and a simultaneous increase in plasma β-mannosidase activity and dramatic decrease in urine-free oligosaccharides were also observed. MRI of the brain remained stable. Neurocognitive evaluation revealed test point gains, although the magnitude of improvement was less than expected for age, causing lower IQ scores that represent a wider developmental gap between the patient and unaffected peers. CONCLUSION: Our findings suggest that hematopoietic cell transplant can correct the biochemical defect in β-mannosidosis, although preservation of the neurocognitive trajectory may be a challenge

Folding Of Xylan Onto Cellulose Fibrils In Plant Cell Walls Revealed By Solid-state Nmr

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Exploitation of plant lignocellulosic biomass is hampered by our ignorance of the molecular basis for its properties such as strength and digestibility. Xylan, the most prevalent non-cellulosic polysaccharide, binds to cellulose microfibrils. The nature of this interaction remains unclear, despite its importance. Here we show that the majority of xylan, which forms a threefold helical screw in solution, flattens into a twofold helical screw ribbon to bind intimately to cellulose microfibrils in the cell wall. C-13 solid-state magic-angle spinning (MAS) nuclear magnetic resonance (NMR) spectroscopy, supported by in silico predictions of chemical shifts, shows both two-and threefold screw xylan conformations are present in fresh Arabidopsis stems. The twofold screw xylan is spatially close to cellulose, and has similar rigidity to the cellulose microfibrils, but reverts to the threefold screw conformation in the cellulose-deficient irx3 mutant. The discovery that induced polysaccharide conformation underlies cell wall assembly provides new principles to understand biomass properties.7BBSRC Grant via BBSRC Sustainable Bioenergy Cell Wall Sugars Programme [BB/G016240/1]CNPq [159341/2011-6, 206278/2014-4]Royal SocietyLeverhulme Trust grant for the Centre for Natural Material InnovationEPSRCBBSRCUniversity of WarwickBirmingham Science City Advanced Materials ProjectsAdvantage West Midlands (AWM)European Regional Development Fund (ERDF)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Eucalypts as a biofuel feedstock

Eucalypts are both a proven but largely unexplored source of woody biomass for biofuel production. Few of the some 900 species have been evaluated for cropping, yet among them are the most productive and versatile biomass species in the world, grown in over 90 countries, with species found to suit most tropical and temperate climates. The biology, science and technology underlying the breeding and growing of eucalypts and their potential for biofuel production are reviewed. How eucalypts meet sustainability and economic criteria for biofuel feedstocks, and the advantages of woody feedstocks broadly, are considered. Relevant aspects of eucalypt taxonomy, evolution, natural distribution, human dispersal, composition, domestication and biotechnology of the groups' potential as a biofuel feedstock resource are reviewed. Two case studies are outlined, illustrating species identification, domestication and harvesting processes where eucalypts are prospective biofuel feedstocks. Eucalypts are strong contenders as a universal woody biomass feedstock for biofuel

Probing the molecular architecture of Arabidopsis thaliana secondary cell walls using two- and three-dimensional (13)C solid state nuclear magnetic resonance spectroscopy.

The plant secondary cell wall is a thickened polysaccharide and phenolic structure, providing mechanical strength to cells, particularly in woody tissues. It is the main feedstock for the developing bioenergy and green chemistry industries. Despite the role that molecular architecture (the arrangement of biopolymers relative to each other, and their conformations) plays in dictating biomass properties, such as recalcitrance to breakdown, it is poorly understood. Here, unprocessed dry (13)C-labeled stems from the model plant Arabidopsis thaliana were analyzed by a variety of (13)C solid state magic angle spinning nuclear magnetic resonance methods, such as one-dimensional cross-polarization and direct polarization, two-dimensional refocused INADEQUATE, RFDR, PDSD, and three-dimensional DARR, demonstrating their viability for the study of native polymer arrangements in intact secondary cell walls. All carbon sites of the two main glucose environments in cellulose (previously assigned to microfibril surface and interior residues) are clearly resolved, as are carbon sites of the other major components of the secondary cell wall: xylan and lignin. The xylan carbon 4 chemical shift is markedly different from that reported previously for solution or primary cell wall xylan, indicating significant changes in the helical conformation in these dried stems. Furthermore, the shift span indicates that xylan adopts a wide range of conformations in this material, with very little in the 31 conformation typical of xylan in solution. Additionally, spatial connections of noncarbohydrate species were observed with both cellulose peaks conventionally assigned as "surface" and as "interior" cellulose environments, raising questions about the origin of these two cellulose signals.This work was supported by BBSRC Grant BB/G016240/1, via The BBSRC Sustainable Bioenergy Cell Wall Sugars Programme. The UK 850 MHz solid state NMR Facility was funded by EPSRC Grant EP/F017901/1 and the BBSRC, as well as the University of Warwick, including via partial funding through Birmingham Science City Advanced Materials Projects 1 and 2, by Advantage West Midlands (AWM) and the European Regional Development Fund (ERDF).This is the final published version. It first appeared at http://pubs.acs.org/doi/abs/10.1021/bi501552k

Enhanced monography in a collaboratively evolved hub for systematic biology

No abstract available