THE ANALYSIS ON RACISM IN 12 YEARS A SLAVE MOVIE

This study discusses a practice of racism carried out by the upper class to the lower class. Racism is behavioral differences and inequalities based on a person's skin color, race, ethnicity, and origin that limit or violate a person's rights and freedoms. Racism is often interpreted as the view that humans can be divided into separate groups based on biological characteristics called "race". The aims of this research are 1) to reveal the practice of racism by the upper class to the lower class., and 2) to show the form of social discrimination due to the practice of racism in the United stated on 19th century. This study uses Goldman's Genetic Structuralism to analyze the film 12 Years a Slave. This analysis produces two forms of racism, namely verbal and non-verbal forms. This form of racism takes place continuously and is maintained by social classes in society

Epidemiological and clinical profile of various plant poisons in a tertiary care center in South Tamilnadu, India

Background: Plant poisoning is a common scenario in a toxicology unit . Some of the plants contain active components used as medicines. However, some of the plant constituents are poisonous with wide variety of clinical manifestations. Authors aimed to study the pattern of various types of plant poisons and clinical presentations and complications of various plant poisons admitted in our toxicological unit.Methods: A total of 87 patients admitted with history of plant poison ingestion, in toxicological unit of Tirunelveli medical college were studied . It was a retrospective observational study and was done over a period of one year.Results:  Out of 87 cases studied, 92%were due to consumption with suicidal intention. Plant poisoning ranks second only to pesticide poisoning . among the plant poisons encountered 66.6% were due to Oleander poison, followed by Datura (8%) and Glory lily(5.7%). Common parts of plant consumed by patients include seeds and plant oils . Vomiting and abdominal pain were the commonest presentations. Encephalopathy, dyselectrolytemia and arrhythmias were the notable complications in our study.Conclusions: Early admission and prompt first aid and monitoring at tertiary care center are key to the reduced mortality. The study throws light on various manifestations, toxic parts and complications of plant poisons

Differences On Students’ Learning Outcomes With High And Low Achievement Motivation

This research aims to examine the difference of students’ learning outcomes as an effect of achievement motivation in writing an English Essay through film-assisted inquiry-based learning method. It was a quasi-experimental research non-equivalent control group design which implemented into two different parallel classes. They are experimental group and control group, in which seventy two students were accepted as subject surveyed through cluster random sampling technique. Data of students’ learning outcomes collected through pre-test and post-test. While data of students’ achievement motivation level collected through questionnaire. The data of pre-test was taken before treatment, used to determine students’ variance, whereas post-test data was taken after treatment entirely finished to examine students’ learning outcomes differences. Data analysis using SPSS 22 with ANOVA Factorial 2x2 (two- way ANOVA. The hypothesis testing was conducted in 5% of significance level. The result of data analysis indicates that the p-value of t-counted is 0.000 lower than 0.05(˂0.05). It can be interpreted that there is a significant difference of learning outcomes between the students who have a high achievement motivation and those who have low one in writing an English essay through film-assisted inquiry-based learning method. Moreover, the students with high achievement motivation have better achievement than the students with low achievement motivation

Study on Haematological Abnormalities in Various stages of Chronic Kidney Disease (stage 3-5)

Chronic kidney disease is one of the major health problems worldwide and a major cause of morbidity and mortality. CKD is diagnosed on the basis of presence of markers of kidney damage and kidney function. This study was done to assess the prevalence hematological abnormalities in CKD (stage3-5) and to assess their correlation among various etiologies of ckd (diabetes, chronic glomerulonephritis, hypertension). The study was done in 150 cases diagnosed as CKD in the Department of Nephrology, IMCU, and in medical ward at Tirunelveli Medical College Hospital. The diagnosis was based on estimated GFR level <60ml/mt/1.73M2. Total count, differential count, Hb, MCV, MCH, MCHC, WBC count, platelet count and peripheral smear examination, coagulation profile were done on all the patients and results were compared and correlated with each other

Various Stages of Immune Synapse Formation Are Differently Dependent on the Strength of the TCR Stimulus

Cytotoxic T lymphocytes (CTL) are key players of the adaptive immune system that target tumors and infected cells. A central step to that is the formation of a cell–cell contact zone between the CTL and its target called an immune synapse (IS). Here, we investigate the influence of the initial T cell receptor (TCR) trigger of a cytolytic IS on the distinct steps leading to cytotoxic granule (CG) exocytosis. We stimulated primary CTLs from mouse using lipid bilayers with varying anti-CD3 but constant ICAM concentrations. We fluorescently labeled molecular markers of distinct IS zones such as actin, CD3, granzyme B, and Synaptobrevin2 in CTLs and imaged cytolytic IS formation by total internal reflection fluorescence microscopy (TIRFM). We found that an intermediate anti-CD3 concentration of 10 µg/mL induces the fastest adhesion of CTLs to the bilayers and results in maximal CG fusion efficiency. The latency of actin ring formation, dwell time, and maximum surface area at the IS exhibit different dependencies on the stimulatory anti-CD3 concentrations. The number and surface area of CD3 clusters at the IS seem to show a different dependency to the TCR trigger when compared to their dwell time. Finally, the mode of full CG exocytosis appears to be independent of the TCR trigger

Redox controls reca protein activity via reversible oxidation of its methionine residues

Reactive oxygen species (ROS) cause damage to DNA and proteins. Here we report that the RecA recombinase is itself oxidized by ROS. Genetic and biochemical analyses revealed that oxidation of RecA altered its DNA repair and DNA recombination activities. Mass spectrometry analysis showed that exposure to ROS converted 4 out of 9 Met residues of RecA to methionine sulfoxide. Mimicking oxidation of Met35 by changing it for Gln caused complete loss of function whereas mimicking oxidation of Met164 resulted in constitutive SOS activation and loss of recombination activity. Yet, all ROS-induced alterations of RecA activity were suppressed by methionine sulfoxide reductases MsrA and MsrB. These findings indicate that under oxidative stress, MsrA/B is needed for RecA homeostasis control. The implication is that, besides damaging DNA structure directly, ROS prevent repair of DNA damage by hampering RecA activity.Agence Nationale de la Re-cherche ANR-10-LABX-62-IBEIDFondation pour la Recherche Medicale FRM - FDT20150532554National Institute of General Medical Sciences GM3233

Literary and Ecological Consciousness: An Ecocriticism Perspective

Environmental issues inspired this research. Only ecological consciousness can minimize environmental damage. This study aims to show that studying literary work is one way that can be taken to overcome environmental problems. The research method is Ricoeur's Hermeneutics method which addresses the interpretation of the signs or symbols contained in the literary text. This research reveals that literary texts contain contents of ecological consciousness, namely literary texts have a spirit to direct humans to become humans who have ecological consciousness. Building ecological consciousness is targeting the wider community. Schools and higher education have an important role in building ecological consciousness

Multiple novel prostate cancer susceptibility signals identified by fine-mapping of known risk loci among Europeans

Genome-wide association studies (GWAS) have identified numerous common prostate cancer (PrCa) susceptibility loci. We have fine-mapped 64 GWAS regions known at the conclusion of the iCOGS study using large-scale genotyping and imputation in 25 723 PrCa cases and 26 274 controls of European ancestry. We detected evidence for multiple independent signals at 16 regions, 12 of which contained additional newly identified significant associations. A single signal comprising a spectrum of correlated variation was observed at 39 regions; 35 of which are now described by a novel more significantly associated lead SNP, while the originally reported variant remained as the lead SNP only in 4 regions. We also confirmed two association signals in Europeans that had been previously reported only in East-Asian GWAS. Based on statistical evidence and linkage disequilibrium (LD) structure, we have curated and narrowed down the list of the most likely candidate causal variants for each region. Functional annotation using data from ENCODE filtered for PrCa cell lines and eQTL analysis demonstrated significant enrichment for overlap with bio-features within this set. By incorporating the novel risk variants identified here alongside the refined data for existing association signals, we estimate that these loci now explain ∼38.9% of the familial relative risk of PrCa, an 8.9% improvement over the previously reported GWAS tag SNPs. This suggests that a significant fraction of the heritability of PrCa may have been hidden during the discovery phase of GWAS, in particular due to the presence of multiple independent signals within the same regio