Role of insulin sensitising agents in altering PSA level in PCOS

Background: Ovarian androgen production can be promoted by insulin resistance which leads to reproductive abnormalities in Polycystic Ovarian Syndrome (PCOS). A wide variety of female tissues can synthesize and secrete Prostate Specific Antigen (PSA). Androgens may take part a significant role in PSA secretion in PCOS. As insulin resistance stimulates androgen production, the baseline value of PSA may decline by insulin sensitising agents in PCOS. Present study is an attempt to measure the function of PSA as a marker of androgen excess in PCOS and to assess the role of insulin sensitising agent metformin in altering PSA level in PCOS.Methods: The study was undertaken to assess the insulin resistance, testosterone and PSA level in 45 women diagnosed as PCOS and 45 healthy controls. Alteration of insulin resistance, serum testosterone and PSA levels by metformin was also analysed.Results: A significant increase in testosterone, PSA level and insulin resistance was observed in PCOS cases when compared with control (p<0.001). When metformin was given for 4 months, improvement in insulin resistance and testosterone level was found in cases, but PSA values observed no change. Correlation was not found linking insulin resistance with PSA level prior to and after therapy.Conclusions: Serum PSA level could be detected in high significant concentration in PCOS women. Various researches explain that insulin resistance and BMI may perhaps control serum PSA level, but our result demonstrate no effect of insulin sensitising agent on serum PSA value

Salinity and macrophyte drive the biogeography of the sedimentary bacterial communities in a brackish water coastal lagoon

Coastal lagoons are represented by steep gradients in physical, chemical, and biological parameters and are regarded as one of the most productive ecosystems in the world. These lagoons are at an intermediate position between the freshwater and marine water systems. Huge amount of influx of organic matter and nutrient load with the freshwater inputs can be seen in such lagoons. The increased influx of organic matter and nutrients fuel in the lagoon increases the chance of eutrophication. The sedimentary microbial communities play an important role in preventing eutrophication by supporting a diverse assemblage of aerobic and anaerobic microbial communities. Considering the importance of sedimentary bacterial communities, numerous studies have investigated their ecological roles and biogeographical patterns in a variety of aquatic ecosystems. Compared to the marine and freshwater ecosystems, estuarine coastal lagoons are highly dynamic, still are poorly understood with respect to their sedimentary communities. Our hypothesis was that bacterial communities would exhibit biogeographical patterns which would be strongly associated with the biotic and abiotic factors. Using Illumina sequencing of the 16S rRNA genes from bulk surface sediments, we investigated the sedimentary bacterial communities, their spatiotemporal distribution, and compared them with the rhizosphere sediment communities of an exotic weed; P. karka and a native seagrass species; H. uninervis in a brackish water estuarine lagoon, Chilika (India). Comparison of bacterial communities with the environmental factor was done using Redundancy analysis. Spatiotemporal patterns in bacterial communities were linked to specific biotic factors (e.g., presence and type of macrophyte) and abiotic factors (e.g., salinity) that drove the community composition. Comparative assessment of communities highlighted bacterial lineages that were responsible for segregating the sediment communities over distinct salinity regimes, seasons, locations, and presence and type of macrophytes. Several bacterial taxa were specific to one of these ecological factors suggesting that species-sorting processes drive specific biogeographical patterns in the bacterial populations. Overall, this study provides a comprehensive understanding of the spatiotemporal dynamics and functionality of sedimentary bacterial communities in a tropical brackish water coastal lagoon and highlighted the role of biotic and abiotic factors in generating the biogeographical patterns in the bacterial communities