Conjugative transfer frequencies of mef(A)-containing Tn1207.3 to macrolide-susceptible Streptococcus pyogenes belonging to different emm types

The aim of this study was to examine the gene transfer potential of mef(A)-containing Tn120.3 to macrolide-susceptible Streptococcus pyogenes belonging to different emm types. Using the filter mating technique, Tn1207.3 was transferred by conjugation to 23 macrolide-susceptible recipients representing 11 emm types. PCR analysis confirmed the presence of the mef(A) gene and the comEC junction regions of the Tn1207.3 insertion in resultant transconjugants. Significant variation was found in the transfer frequency of Tn1207.3 to different Strep. pyogenes strains, and this phenomenon may contribute to the differences in mef(A) frequency observed among clinical isolates. Significance and Impact of the Study: The spread of antimicrobial resistance among pathogenic bacteria is an important problem, but the mechanisms of horizontal transfer between strains and species are often poorly understood. For instance, little is known on how macrolide resistance spreads between strains of the human pathogen Strep. pyogenes and why certain strains more commonly display resistance than others. Here, we show that Strep. pyogenes strains vary greatly in their ability to acquire a transposon encoding macrolide resistance by horizontal gene transfer in vitro. These data provide a novel insight into the transfer of antibiotic resistance between bacterial strains and offer an explanation for the differences in the frequency of resistance determinates and resistance seen among clinical isolates. © 2014 The Authors Letters in Applied Microbiology

Construct validity of the Experiences of Therapy Questionnaire (ETQ)

ObjectiveThe Experiences of Therapy Questionnaire (ETQ) is a reliable measure of adverse effects associated with psychotherapy. The measure has not been subject to validity analyses. This study sought to examine the validity of the ETQ by comparison against a measure of therapist satisfaction.MethodsParticipants were recruited from the Black Dog Institute¿s website and completed all measures online, at two time points (two weeks apart). Correlational analyses compared scale scores on the ETQ with related constructs of the Therapist Satisfaction Scale (TSS). To exclude any impact of current depression on ratings, we examined correlations between salient ETQ and TSS scales after controlling for depression severity.ResultsForty-six participants completed all the measures at both time points. Hypothesised associations between the ETQ and TSS scales were supported, irrespective of current depression severity.ConclusionsThe validity of the ETQ is supported; however limitations of the study are noted, including generalizability due to sample characteristics

Photoproduction of Lambda and Sigma(0) hyperons using linearly polarized photons

Background: Measurements of polarization observables for the reactions (gamma) over right arrowp -\u3e K+ Lambda and (gamma) over right arrowp -\u3e K+ Sigma(0) have been performed. This is part of a program of measurements designed to study the spectrum of baryon resonances in particular, and nonperturbative QCD in general. Purpose: The accurate measurement of several polarization observables provides tight constraints for phenomenological fits, which allow the study of strangeness in nucleon and nuclear systems. Beam-recoil observables for the (gamma) over right arrowp -\u3e K+ Sigma(0) reaction have not been reported before now. Method: Themeasurements were carried out using linearly polarized photon beams incident on a liquid hydrogen target, and the CLAS detector at the Thomas Jefferson National Accelerator Facility. The energy range of the results is 1.71 \u3c W \u3c 2.19 GeV, with an angular range -0.75 \u3c cos theta(K)* \u3c +0.85. Results: The observables extracted for both reactions are beam asymmetry Sigma, target asymmetry T, and the beam-recoil double polarization observables O-x and O-z. Conclusions: Comparison with theoretical fits indicates that, in the regions where no previous data existed, the new data contain significant new information, and strengthen the evidence for the set of resonances used in the latest Bonn-Gatchina fit

Short communication: Biofilm production characterization of mecA and mecC methicillin-resistant Staphylococcus aureus isolated from bovine milk in Great Britain

Staphylococcus aureus is an important cause of contagious intramammary infection in dairy cattle and the ability to produce biofilm is considered to be an important virulence property in the pathogenesis of mastitis. The aim of this study was to characterize the biofilm formation capacity of methicillin-resistant S. aureus (MRSA), encoding mecA or mecC, isolated from bulk tank milk in Great Britain. For this purpose, 20 MRSA isolates were grown on microtiter plates to determine the biofilm production. Moreover, the presence of the intercellular adhesion genes icaA and icaD were analyzed by PCR. All the tested MRSA isolates were PCR positive for the ica genes and 10 of them (50%) produced biofilm in the microtiter plate assay. This is also the first demonstration of biofilm production by mecC MRSA

Re-Inventing Public Education:The New Role of Knowledge in Education Policy-Making

This article focuses on the changing role of knowledge in education policy making within the knowledge society. Through an examination of key policy texts, the Scottish case of Integrated Children Services provision is used to exemplify this new trend. We discuss the ways in which knowledge is being used in order to re-configure education as part of a range of public services designed to meet individuals' needs. This, we argue, has led to a 'scientization' of education governance where it is only knowledge, closely intertwined with action (expressed as 'measures') that can reveal problems and shape solutions. The article concludes by highlighting the key role of knowledge policy and governance in orienting education policy making through a re-invention of the public role of education

The OmegaWhite Survey for Short-Period Variable Stars IV: Discovery of the warm DQ white dwarf OW J175358.85-310728.9

We present the discovery and follow-up observations of the second known variable warm DQ white dwarf OW J175358.85-310728.9 (OW J1753-3107). OW J1753-3107 is the brightest of any of the currently known warm or hot DQ and was discovered in the OmegaWhite Survey as exhibiting optical variations on a period of 35.5452 (2) mins, with no evidence for other periods in its light curves. This period has remained constant over the last two years and a single-period sinusoidal model provides a good fit for all follow-up light curves. The spectrum consists of a very blue continuum with strong absorption lines of neutral and ionised carbon, a broad He I 4471 A line, and possibly weaker hydrogen lines. The C I lines are Zeeman split, and indicate the presence of a strong magnetic field. Using spectral Paschen-Back model descriptions, we determine that OW J1753-3107 exhibits the following physical parameters: T_eff = 15430 K, log(g) = 9.0, log(N(C)/N(He)) = -1.2, and the mean magnetic field strength is B_z =2.1 MG. This relatively low temperature and carbon abundance (compared to the expected properties of hot DQs) is similar to that seen in the other warm DQ SDSS J1036+6522. Although OW J1753-3107 appears to be a twin of SDSS J1036+6522, it exhibits a modulation on a period slightly longer than the dominant period in SDSS J1036+6522 and has a higher carbon abundance. The source of variations is uncertain, but they are believed to originate from the rotation of the magnetic white dwarf.Comment: 11 pages, 8 figures, 7 tables. Accepted for publication by MNRA

Optimising Antibiotic Usage to Treat Bacterial Infections

The increase in antibiotic resistant bacteria poses a threat to the continued use of antibiotics to treat bacterial infections. The overuse and misuse of antibiotics has been identified as a significant driver in the emergence of resistance. Finding optimal treatment regimens is therefore critical in ensuring the prolonged effectiveness of these antibiotics. This study uses mathematical modelling to analyse the effect traditional treatment regimens have on the dynamics of a bacterial infection. Using a novel approach, a genetic algorithm, the study then identifies improved treatment regimens. Using a single antibiotic the genetic algorithm identifies regimens which minimise the amount of antibiotic used while maximising bacterial eradication. Although exact treatments are highly dependent on parameter values and initial bacterial load, a significant common trend is identified throughout the results. A treatment regimen consisting of a high initial dose followed by an extended tapering of doses is found to optimise the use of antibiotics. This consistently improves the success of eradicating infections, uses less antibiotic than traditional regimens and reduces the time to eradication. The use of genetic algorithms to optimise treatment regimens enables an extensive search of possible regimens, with previous regimens directing the search into regions of better performance

perceiving animacy and arousal in transformed displays of human interaction

When viewing a moving abstract stimulus, people tend to attribute social meaning and purpose to the movement. The classic work of Heider and Simmel [1] investigated how observers would describe movement of simple geometric shapes (circle, triangles, and a square) around a screen. A high proportion of participants reported seeing some form of purposeful interaction between the three abstract objects and defining this interaction as a social encounter. Various papers have subsequently found similar results [2,3] and gone on to show that, as Heider and Simmel suggested, the phenomenon was due more to the relationship in space and time of the objects, rather than any particular object characteristic. The research of Tremoulet and Feldman [4] has shown that the percept of animacy may be elicited with a solitary moving object. They asked observers to rate the movement of a single dot or rectangle for whether it was under the influence of an external force, or whether it was in control of its own motion. At mid-trajectory the shape would change speed or direction, or both. They found that shapes that either changed direction greater than 25 degrees from the original trajectory, or changed speed, were judged to be "more alive" than others. Further discussion and evidence of animacy with one or two small dots can be found in Gelman, Durgin and Kaufman [5] Our aim was to further study this phenomenon by using a different method of stimulus production. Previous methods for producing displays of animate objects have relied either on handcrafted stimuli or on parametric variations of simple motion patterns. It is our aim to work towards a new automatic approach by taking actual human movements, transforming them into basic shapes, and exploring what motion properties need to be preserved to obtain animacy. Though the phenomenon of animacy has been shown for many years, using various different displays, very few specific criteria have been set on the essential characteristics of the displays. Part of this research is to try and establish what movements result in percepts of animacy, and in turn, to give further understanding of essential characteristics of human movement and social interaction. In this paper we discuss two experiments in which we examine how different transformations of an original video of a dance influences perception of animacy. We also examine reports of arousal, Experiment 1, and emotional engagement in Experiment 2