Correlation of serum lactate dehydrogenase and pregnancy induced hypertension with its adverse outcomes

Background: Pregnancy induced hypertension (PIH) is a global problem with a 5-15% incidence rate in India and complicating 10-17% of all pregnancies. The complications of PIH are responsible for significant maternal and perinatal morbidity and are the third leading cause of pregnancy related death. Lactate dehydrogenase (LDH) serves as indicators suggestive of disturbance of cellular integrity induced by pathological conditions and is used to detect cell damage or cell death. The main objective was to assess significance of the value of serum LDH as a marker of PIH and its severity.Methods: Serum LDH was analyzed by modified UV Kinetic IFCC method. This study was conducted from February 2014 to June 2015 and all the patients admitted in the department of obstetrics and gynecology and biochemistry, at the GMERS medical college, Dharpur, Patan, Gujarat, India.Results: Total 110 cases were studied during the study period, 40 were normal pregnant women and remaining 70 were PIH cases. Out of the 70 PIH cases, 15 (21.5%) were mild preecclampsia, 35 (50.0%) were severe preeclampsia and 20 (28.5%) were eclampsia. Maternal mortality occurred in 06 cases (8.5%). Perinatal mortality was seen in 28 (40.0%), Out of these, 20 (71.4%) were stillbirth and 08 (28.6%) were neonatal deaths. There is significant rise in the LDH levels with the increasing severity of the disease (172.37±28.09) normotensive, (356.33±24.47) mild preeclampsia, (609.91±136.92) severe preeclampsia and (854.05±247.45), eclampsia (P800 IU/l.Conclusions: Serum LDH showed significant association with severity of disease and maternal and fetal complications in patients with preclampsia-eclamapsia

A comparative quantitative & qualitative assessment in orthodontic treatment of white spot lesion treated with 3 different commercially available materials - In vitro study

To comparatively evaluate the esthetic improvement of white-spot lesions (WSLs) treated by: BiominF, CPP-ACP paste with fluoride & ICON resin infiltration, using Spectrophotometer & Diagnodent. The study was done using 72 sound permanent extracted premolars, divided into four groups (18 teeth per group). After taking the ethical approval the study was commenced. WSLs were created on human premolars and randomly assigned to four groups: Group A: Artificial Saliva, Group B: CPP-ACP with fluoride, Group C: BiominF, Group D: Resin infiltration (Icon). The color change (?E) of each specimen was measured with a Spectrophotometer (VITA Easy Shade Compact), and fluorescence loss (?Q) was measured by a laser fluorescence device (DIAGNOdent, Kavo, Biberach, Germany), at different time points after treatment: baseline (0 weeks), 2 weeks, 4 weeks, and 6 weeks. The ?E and ?Q baseline values for the four groups before the treatments did not differ significantly. Icon treatment improved the WSL color significantly and gave the lowest ?E (5.12± 3.92) & ?Q (1.64 ±0.72) compared with other treatments at end of 6 weeks (P< .01). In the BiominF and CPP-ACP with fluoride treatment groups, ?Q & ?E showed significant recovery compared with the baseline values (P< .05). Within the limitations of the study, it can be concluded that all the three remineralizing agents used in the study could effectively remineralize artificial enamel caries and showed improvement in color change and fluoresence as compared to the baseline. Therefore they can be effectively used for the treatment of the white spot lesions

EDTA contamination: a preanalytical cause for interference in iron and unsaturated iron binding capacity assay

Background: The aim of the study is focussed very keenly at performing RCA (root cause analysis) of those particular sample containing the absurd results of the Serum UIBC (Unsaturated Iron Binding Capacity), which was also tallied vigilantly side by side with Serum Iron observed at Clinical Biochemistry laboratory of The New Civil Hospital Surat, Gujarat.Methods: The Absurd value from the samples requested for Serum Iiron and Serum Serum UIBC (Unsaturated Iron Binding Capacity) from month of August 2018 till the month of August 2019 were taken in to account for analysis. RCA (root cause analysis) of absurd value for Serum Iron and Serum UIBC (Unsaturated Iron Binding Capacity) which were prepared was mainly focused on tallying with Serum Calcium and Serum Potassium result.Results: With the continious and strenous monitoring from the side of the researchers, the researchers had throughly analysed and found that in almost all of those analysed test containing absurd results of Serum Iron and Serum UIBC(Unsaturated Iron Binding Capacity) which were some way or the other, associated with absurd results of Serum Calcium along with absurd result of Serum Potassium too from the same samples.Conclusions: The Absurd results of Serum Calcium and Serum Potassium are caused mainly due to pre-analytical errors more likely due to the sample contamination with EDTA (ethylene diamine tetra acetic acid). The contaminated EDTA (ethylene diamine tetra acetic acid) sample cause interference in Serum Iron measurement by producing turbidity in sample and in Serum UIBC (Unsaturated Iron Binding Capacity) by chelating Iron

Accelerated Testing of Bearings for High Speed Application

In this research thesis, a new method for accelerated lifetime testing (ALT) of high-speed bearings is discussed. The rapid development of products, and the longer lifespan of bearings on high-speed applications, have generated demand for the accelerated testing method.A new method is explained for ALT of the bearings. The life of the bearings is reduced by producing higher external force on them. This external force is produced as a result of the gyroscopic couple on a newly developed test bench.This research paper describes various tests for the validation of the new method of ALT of bearings. Furthermore, experiments are discussed for monitoring the health status of bearings by analysing the electrical current of the Brushless Direct Current motor that is running the bearings in the product

Matlab Simulation of Single Phase Shunt Active Filter Based on PQ Theory

ABSTRACT- This paper presents the simulation of single phase shunt active filter based on PQ theory. Generally PQ theory is used for 3 phases 3 wires or 3 phases 4 wire system but here it is used for single phase system. This paper also concentrates hardly on reduction of THD of load current. Since the system has only single phase signal for both voltage and current, thus the dummy signal with 120 º different angels must be generated for input of the p-q theory. During simulation the six pulses will be generated for switching of IGBT but only two will be used. MATLAB/SIMULINK power system toolbox is used to simulate the proposed system

Physiologically based pharmacokinetic modeling of transdermal selegiline and its metabolites for the evaluation of disposition differences between healthy and special populations

A physiologically based pharmacokinetic (PBPK) model of selegiline (SEL), and its metabolites, was developed in silico to evaluate the disposition differences between healthy and special populations. SEL is metabolized to methamphetamine (MAP) and desmethyl selegiline (DMS) by several CYP enzymes. CYP2D6 metabolizes the conversion of MAP to amphetamine (AMP), while CYP2B6 and CYP3A4 predominantly mediate the conversion of DMS to AMP. The overall prediction error in simulated PK, using the developed PBPK model, was within 0.5–1.5-fold after intravenous and transdermal dosing in healthy and elderly populations. Simulation results generated in the special populations demonstrated that a decrease in cardiac output is a potential covariate that affects the SEL exposure in renally impaired (RI) and hepatic impaired (HI) subjects. A decrease in CYP2D6 levels increased the systemic exposure of MAP. DMS exposure increased due to a reduction in the abundance of CYP2B6 and CYP3A4 in RI and HI subjects. In addition, an increase in the exposure of the primary metabolites decreased the exposure of AMP. No significant difference between the adult and adolescent populations, in terms of PK, were observed. The current PBPK model predictions indicate that subjects with HI or RI may require closer clinical monitoring to identify any untoward effects associated with the administration of transdermal SEL patch

Characterising the Analgesic Effect of Different Targets for Deep Brain Stimulation in Trigeminal Anaesthesia Dolorosa

BACKGROUND: Several deep brain stimulation (DBS) targets have been explored for the alleviation of trigeminal anaesthesia dolorosa. We aimed to characterise the analgesia produced from the periaqueductal grey (PAG) and centromedian-parafascicular (CmPf) nucleus using a within-subject design. METHOD: We report a case series of 3 subjects implanted with PAG and CmPf DBS systems for the treatment of anaesthesia dolorosa. At follow-up, testing of onset and offset times, magnitude, and thermal and mechanical sensitivity was performed. RESULTS: The mean pain score of the cohort was acutely reduced by 56% (p < 0.05) with PAG and 67% (p < 0.01) with CmPf stimulation at mean time intervals of 38 and 16 min, respectively. The onset time was 12.5 min (p < 0.05) for PAG stimulation and 2.5 min (p < 0.01) for CmPf. The offset time was 2.5 min (p < 0.05) for PAG and 12.5 min (p < 0.01) for CmPf. The two targets were effective at different stimulation frequencies and were not antagonistic in effect. CONCLUSION: The mechanisms by which stimulation at these two targets produces analgesia are likely to be different. Certain pain qualities may respond more favourably to specific targets. Knowledge of onset and offset times for the targets can guide optimisation of stimulation settings. The use of more than one stimulation target may be beneficial and should be considered in anaesthesia dolorosa patients

Assessment of diurnal variation in Ocimum sanctum Linn. by gas chromatographic fingerprint analysis coupled with chemometric methods

Vast intra-specific variations, especially diurnal, geographical and seasonal, have been reported in the chemical composition of essential oils of Ocimum species. The study was conducted to assess diurnal variation in the chemical composition of the leaves of Ocimum sanctum. The leaf samples collected at different times of the day were analyzed by gas chromatography coupled with flame ionization detector (GC-FID). The chromatographic fingerprints of different leaf samples were analyzed by chemometric methods like principal component analysis and hierarchical cluster analysis. No significant difference was found in the chemical compositions of the leaf samples collected at different times of the day. The results lead to a conclusion that O. sanctum does not exhibit diurnal variation in its chemical composition, unlike O. gratissimum