1,003 research outputs found
Reconstructed Living Lab: supporting drug users and families through co-operative counselling using mobile phone technology
Background: There is a recognised problem with drug taking in South Africa. In socially deprived areas immediate help for drug users and their families is a problem. As part of their work in a community in tension, Impact Direct Ministries (IDM) and Reconstructed Living Lab (RLabs) in Cape Town provide a drug advisory service using mobile phone technology that can support multiple conversations. It is staffed by trained volunteers and is available to drug users and their families. Methods: This article investigates historical counselling help for drug users. It explains the importance of family involvement in the life-changing process of a drug user and the importance of co-operative counselling. The Drug Advice Support (DAS) service provided by IDM and RLabs is introduced as a case study to explore how mobile phone technology can support the co-operative counselling model in a Living Lab context. Results: The advantages of the DAS technology and what it offers to community-based organisations are discussed. Data on relatives of drug users using the system are included. Conclusion: The use of mobile phone technology has advantages for community-based organisations acting as a first point of contact to drug users and their families. Minimal cost to the person in crisis and the organisation serves as an example. The co-operative counselling model it employs is also of benefit. As the community experiencing tension due to the problem of drug abuse becomes aware of this service, help and support for family members will increase in time.Keywords: community-based organisation; Living Labs; drug users’ families; co-operative counselling; mobile phone
Global urinary volatolomics with (GC×)GC-TOF-MS
Urinary volatolomics offers a noninvasive approach for disease detection and monitoring. Herein we present an improved methodology for global volatolomic profiling. Wide coverage was achieved by utilizing a multiphase sorbent for volatile organic compound (VOC) extraction. A single, midpolar column gas chromatography (GC) assay yielded substantially higher numbers of monitored VOCs compared to our previously reported single-sorbent method. Multidimensional GC (GC×GC) enhanced further biomarker discovery while data analysis was simplified by using a tile-based approach. At the same time, the required urine volume was reduced 5-fold from 2 to 0.4 mL. The applicability of the methodology was demonstrated in a pancreatic ductal adenocarcinoma cohort where previous findings were confirmed while a series of additional VOCs with diagnostic potential were discovered
On Convergence and Threshold Properties of Discrete Lotka-Volterra Population Protocols
In this work we focus on a natural class of population protocols whose
dynamics are modelled by the discrete version of Lotka-Volterra equations. In
such protocols, when an agent of type (species) interacts with an agent
of type (species) with as the initiator, then 's type becomes
with probability . In such an interaction, we think of as the
predator, as the prey, and the type of the prey is either converted to that
of the predator or stays as is. Such protocols capture the dynamics of some
opinion spreading models and generalize the well-known Rock-Paper-Scissors
discrete dynamics. We consider the pairwise interactions among agents that are
scheduled uniformly at random. We start by considering the convergence time and
show that any Lotka-Volterra-type protocol on an -agent population converges
to some absorbing state in time polynomial in , w.h.p., when any pair of
agents is allowed to interact. By contrast, when the interaction graph is a
star, even the Rock-Paper-Scissors protocol requires exponential time to
converge. We then study threshold effects exhibited by Lotka-Volterra-type
protocols with 3 and more species under interactions between any pair of
agents. We start by presenting a simple 4-type protocol in which the
probability difference of reaching the two possible absorbing states is
strongly amplified by the ratio of the initial populations of the two other
types, which are transient, but "control" convergence. We then prove that the
Rock-Paper-Scissors protocol reaches each of its three possible absorbing
states with almost equal probability, starting from any configuration
satisfying some sub-linear lower bound on the initial size of each species.
That is, Rock-Paper-Scissors is a realization of a "coin-flip consensus" in a
distributed system. Some of our techniques may be of independent value
Theoretical Models of Sunspot Structure and Dynamics
Recent progress in theoretical modeling of a sunspot is reviewed. The
observed properties of umbral dots are well reproduced by realistic simulations
of magnetoconvection in a vertical, monolithic magnetic field. To understand
the penumbra, it is useful to distinguish between the inner penumbra, dominated
by bright filaments containing slender dark cores, and the outer penumbra, made
up of dark and bright filaments of comparable width with corresponding magnetic
fields differing in inclination by some 30 degrees and strong Evershed flows in
the dark filaments along nearly horizontal or downward-plunging magnetic
fields. The role of magnetic flux pumping in submerging magnetic flux in the
outer penumbra is examined through numerical experiments, and different
geometric models of the penumbral magnetic field are discussed in the light of
high-resolution observations. Recent, realistic numerical MHD simulations of an
entire sunspot have succeeded in reproducing the salient features of the
convective pattern in the umbra and the inner penumbra. The siphon-flow
mechanism still provides the best explanation of the Evershed flow,
particularly in the outer penumbra where it often consists of cool, supersonic
downflows.Comment: To appear in "Magnetic Coupling between the Interior and the
Atmosphere of the Sun", eds. S.S. Hasan and R.J. Rutten, Astrophysics and
Space Science Proceedings, Springer-Verlag, Heidelberg, Berlin, 200
A complete pipeline for untargeted urinary volatolomic profiling with sorptive extraction and dual polar and nonpolar column methodologies coupled with gas chromatography time-of-flight mass spectrometry.
Volatolomics offers an opportunity for noninvasive detection and monitoring of human disease. While gas chromatography-mass spectrometry (GC-MS) remains the technique of choice for analyzing volatile organic compounds (VOCs), barriers to wider adoption in clinical practice still exist, including: sample preparation and introduction techniques, VOC extraction, throughput, volatolome coverage, biological interpretation, and quality control (QC). Therefore, we developed a complete pipeline for untargeted urinary volatolomic profiling. We optimized a novel extraction technique using HiSorb sorptive extraction, which exhibited high analytical performance and throughput. We achieved a broader VOC coverage by using HiSorb coupled with a set of complementary chromatographic methods and time-of-flight mass spectrometry. Furthermore, we developed a data preprocessing strategy by evaluating internal standard normalization, batch correction, and we adopted strict QC measures including removal of nonlinearly responding, irreproducible, or contaminated metabolic features, ensuring the acquisition of high-quality data. The applicability of this pipeline was evaluated in a clinical cohort consisting of pancreatic ductal adenocarcinoma (PDAC) patients (n = 28) and controls (n = 33), identifying four urinary candidate biomarkers (2-pentanone, hexanal, 3-hexanone, and p-cymene), which can successfully discriminate the cancer and noncancer subjects. This study presents an optimized, high-throughput, and quality-controlled pipeline for untargeted urinary volatolomic profiling. Use of the pipeline to discriminate PDAC from control subjects provides proof of principal of its clinical utility and potential for application in future biomarker discovery studies
Origin of Cosmic Magnetic Fields
We propose that the overlapping shock fronts from young supernova remnants
produce a locally unsteady, but globally steady large scale spiral shock front
in spiral galaxies, where star formation and therefore massive star explosions
correlate geometrically with spiral structure. This global shock front with its
steep gradients in temperature, pressure and associated electric fields will
produce drifts, which in turn give rise to a strong sheet-like electric
current, we propose. This sheet current then produces a large scale magnetic
field, which is regular, and connected to the overall spiral structure. This
rejuvenates the overall magnetic field continuously, and also allows to
understand that there is a regular field at all in disk galaxies. This proposal
connects the existence of magnetic fields to accretion in disks. We not yet
address all the symmetries of the magnetic field here; the picture proposed
here is not complete. X-ray observations may be able to test it already.Comment: 18 pages, no figures; to be published in Proc. Palermo Meeting Sept.
2002, Eds. N. G. Sanchez et al., The Early Universe and the Cosmic Microwave
Background: Theory and Observation
The prevalence of bipolar disorders in the general population - a growing trending topic?
Bipolar disorder prevalence: a systematic review and meta-analysis of the literature. [Rev Bras Psiquiatr. 2015
Removing exogenous information using pedigree data
Management of certain populations requires the preservation of its pure genetic background. When, for different reasons, undesired alleles are introduced, the original genetic conformation must be recovered. The present study tested, through computer simulations, the power of recovery (the ability for removing the foreign information) from genealogical data. Simulated scenarios comprised different numbers of exogenous individuals taking partofthe founder population anddifferent numbers of unmanaged generations before the removal program started. Strategies were based on variables arising from classical pedigree analyses such as founders? contribution and partial coancestry. The ef?ciency of the different strategies was measured as the proportion of native genetic information remaining in the population. Consequences on the inbreeding and coancestry levels of the population were also evaluated. Minimisation of the exogenous founders? contributions was the most powerful method, removing the largest amount of genetic information in just one generation.However, as a side effect, it led to the highest values of inbreeding. Scenarios with a large amount of initial exogenous alleles (i.e. high percentage of non native founders), or many generations of mixing became very dif?cult to recover, pointing out the importance of being careful about introgression events in populatio
A putative biomarker signature for clinically effective AKT inhibition: correlation of in vitro, in vivo and clinical data identifies the importance of modulation of the mTORC1 pathway
Our identification of dysregulation of the AKT pathway in ovarian cancer as a platinum resistance specific event led to a comprehensive analysis of in vitro, in vivo and clinical behaviour of the AKT inhibitor GSK2141795. Proteomic biomarker signatures correlating with effects of GSK2141795 were developed using in vitro and in vivo models, well characterised for related molecular, phenotypic and imaging endpoints. Signatures were validated in temporally paired biopsies from patients treated with GSK2141795 in a clinical study. GSK2141795 caused growth-arrest as single agent in vitro, enhanced cisplatin-induced apoptosis in vitro and reduced tumour volume in combination with platinum in vivo. GSK2141795 treatment in vitro and in vivo resulted in ~50-90% decrease in phospho-PRAS40 and 20-80% decrease in fluoro-deoxyglucose (FDG) uptake. Proteomic analysis of GSK2141795 in vitro and in vivo identified a signature of pathway inhibition including changes in AKT and p38 phosphorylation and total Bim, IGF1R, AR and YB1 levels. In patient biopsies, prior to treatment with GSK2141795 in a phase 1 clinical trial, this signature was predictive of post-treatment changes in the response marker CA125. Development of this signature represents an opportunity to demonstrate the clinical importance of AKT inhibition for re-sensitisation of platinum resistant ovarian cancer to platinum
Chandrasekhar-Kendall functions in astrophysical dynamos
Some of the contributions of Chandrasekhar to the field of
magnetohydrodynamics are highlighted. Particular emphasis is placed on the
Chandrasekhar-Kendall functions that allow a decomposition of a vector field
into right- and left-handed contributions. Magnetic energy spectra of both
contributions are shown for a new set of helically forced simulations at
resolutions higher than what has been available so far. For a forcing function
with positive helicity, these simulations show a forward cascade of the
right-handed contributions to the magnetic field and nonlocal inverse transfer
for the left-handed contributions. The speed of inverse transfer is shown to
decrease with increasing value of the magnetic Reynolds number.Comment: 10 pages, 5 figures, proceedings of the Chandrasekhar Centenary
Conference, to be published in PRAMANA - Journal of Physic
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