Novel Interaction, Regulation of Phosphorylation and Mitotic Localization of Cell Division Cycle Associated Protein 7 (CDCA7)

Cell Division Cycle Associated Protein 7 (CDCA7) is a novel protein with poorly defined physiological significance. The proximity labeling method known as Biotin Identification (BioID) was used to determine candidate interactors of CDCA7. Mass spectrometry identified the microtubule spindle assembly protein TPX2 as a novel candidate interactor of CDCA7. Immunoprecipitation experiments verified interaction between CDCA7 and TPX2. The region of interaction was isolated to residues 195-204 of CDCA7. Based on inhibitor experiments, Aurora A kinase activity coincides with the phosphorylation of CDCA7 at the threonine (T) 163 residue. During mitosis, CDCA7 colocalizes with TPX2, localizes at centrosomes and undergoes phosphorylation at T163. These findings and previous findings in literature place CDCA7, TPX2 and potentially Aurora A kinase together within the context of mitosis. The results here demonstrate the direct interaction between CDCA7 and TPX2, and highlight residue T163 of CDCA7 as a prospective phosphorylation target of Aurora A kinase

Network analysis of depressive symptoms in Hong Kong residents during the COVID-19 pandemic

In network theory depression is conceptualized as a complex network of individual symptoms that influence each other, and central symptoms in the network have the greatest impact on other symptoms. Clinical features of depression are largely determined by sociocultural context. No previous study examined the network structure of depressive symptoms in Hong Kong residents. The aim of this study was to characterize the depressive symptom network structure in a community adult sample in Hong Kong during the COVID-19 pandemic. A total of 11,072 participants were recruited between 24 March and 20 April 2020. Depressive symptoms were measured using the Patient Health Questionnaire-9. The network structure of depressive symptoms was characterized, and indices of “strength”, “betweenness”, and “closeness” were used to identify symptoms central to the network. Network stability was examined using a case-dropping bootstrap procedure. Guilt, Sad Mood, and Energy symptoms had the highest centrality values. In contrast, Concentration, Suicide, and Sleep had lower centrality values. There were no significant differences in network global strength (p = 0.259), distribution of edge weights (p = 0.73) and individual edge weights (all p values > 0.05 after Holm–Bonferroni corrections) between males and females. Guilt, Sad Mood, and Energy symptoms were central in the depressive symptom network. These central symptoms may be targets for focused treatments and future psychological and neurobiological research to gain novel insight into depression

Primary and secondary roof seals : emission control for storage of gasoline in a floating roof tank

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Relationship between physical, chemical and processing properties of rice

Due to the character of the original source materials and the nature of batch digitization, quality control issues may be present in this document. Please report any quality issues you encounter to [email protected], referencing the URI of the item.Not availabl

Antimutagenecity potential of isolates from the chloroform extract of Bidens Pilosa Vidal

Air dried leaves of Bidens Pilosa V. were ground and soaked in chloroform, after which the chloroform extract was concentrated in vacuo leaving a crude extract. The resulting extract was subjected to gravity liquid chromatography (GLC). Fractions obtained from the GLC were monitored by thin layer chromatography (TLC). The fractions were rechromatographed until the isolates were obtained. The isolates including a novel diterpene from Ms. Carmelita Zulueta were tested for possible antimutagenic activity by micronucleus test. Results of the study indicated that the isolates have antimutagenic properties

Pilot testing of Dual-task Zumba Gold (DTZ) for community-dwelling people with mild cognitive impairment : a mixed-methods study

202112 bcvcNot applicableRGCReference No.: PF18-22792Published12 month

E-service Usability and Citizens Expectation : A study on Bangladesh E-government Service

In twentieth century we have witnessed an extensive growth of internet based services in our every sphere of life. The use of information technology has been a core element using by the government as a communication way to its citizen. E-services delivery is present in developing countries but the erudition is still in immaturity level. Most local governments only provide published information and downloadable forms. Most local government sites provide only one way communications. All are still at the basic level of publishing information online. Before implementing and developing any e-services, the authorities should know the expectation to the service from the users and it is also important to know the usability of the e-service in order to develop it with more usability function so that the users will satisfy by using the service. This study is designed to take descriptive look at whether three e-services of Bangladesh government are perceived usable by the citizens. The studies focused on the current usability level of the three e-services and also find out the citizens expectation from those services. The overall conclusion is about the usability level of the e-services, expectations from the citizens, and suggesting the usability criteria to make the e-service more usable.Program: Magisterutbildning i informati