Ultraviolet light curves of U Geminorum and VW Hydri

Ultraviolet light curves were obtained for the quiescent dwarf novae U Gem and VW Hyi. The amplitude of the hump associated with the accretion hot spot is much smaller in the UV than in the visible. This implies that the bright spot temperature is roughly 12000 K if it is optically thick. The flux distribution of U Gem in quiescence cannot be fitted by model spectra of steady state, viscous accretion disks. The absolute luminosity, the flux distribution, and the far UV spectrum suggest that the primary star is visible in the far UV. The optical UV flux distribution of VW Hyi can be matched roughly by the model accretion disks

The 1981 outburst of the old nova GK Persei

Old nova GK Per was observed in 1981 with the IUE, during its rise, maximum, and subsequent return to minimum. In outburst, GK Per is luminous but much redder than dwarf novae or standard model accretion disks. The observed spectrum can be explained qualitatively with the Ghosh and Lamb (1979) model for the interaction of an accretion disk with the magnetic field of the accreting white dwarf. The N V and He2 are enhanced relative to other emission lines during outburst. This can be understood with photoionization by very soft X-rays having a luminosity comparable to that of the hard X-rays

Cosmological CMBR dipole in open universes ?

The observed CMBR dipole is generally interpreted as a Doppler effect arising from the motion of the Earth relative to the CMBR frame. An alternative interpretation, proposed in the last years, is that the dipole results from ultra-large scale isocurvature perturbations. We examine this idea in the context of open cosmologies and show that the isocurvature interpretation is not valid in an open universe, unless it is extremely close to a flat universe, $|\Omega_0 -1|< 10^{-4}$.Comment: 26 pages, Latex, 6 figures, to appear in Phys. Rev.

Utilization of Never-Medicated Bipolar Disorder Patients towards Development and Validation of a Peripheral Biomarker Profile

There are currently no biological tests that differentiate patients with bipolar disorder (BPD) from healthy controls. While there is evidence that peripheral gene expression differences between patients and controls can be utilized as biomarkers for psychiatric illness, it is unclear whether current use or residual effects of antipsychotic and mood stabilizer medication drives much of the differential transcription. We therefore tested whether expression changes in first-episode, never-medicated BPD patients, can contribute to a biological classifier that is less influenced by medication and could potentially form a practicable biomarker assay for BPD. We employed microarray technology to measure global leukocyte gene expression in first-episode (n=3) and currently medicated BPD patients (n=26), and matched healthy controls (n=25). Following an initial feature selection of the microarray data, we developed a cross-validated 10-gene model that was able to correctly predict the diagnostic group of the training sample (26 medicated patients and 12 controls), with 89% sensitivity and 75% specificity (p<0.001). The 10-gene predictor was further explored via testing on an independent cohort consisting of three pairs of monozygotic twins discordant for BPD, plus the original enrichment sample cohort (the three never-medicated BPD patients and 13 matched control subjects), and a sample of experimental replicates (n=34). 83% of the independent test sample was correctly predicted, with a sensitivity of 67% and specificity of 100% (although this result did not reach statistical significance). Additionally, 88% of sample diagnostic classes were classified correctly for both the enrichment (p=0.015) and the replicate samples (p<0.001). We have developed a peripheral gene expression biomarker profile, that can classify healthy controls from patients with BPD receiving antipsychotic or mood stabilizing medication, which has both high sensitivity and specificity. Moreover, assay of three first-episode patients who had never received such medications, to first enrich the expression dataset for disease-related genes independent of medication effects, and then to test the 10-gene predictor, validates the peripheral biomarker approach for BPD

Particle physics with a laser-driven positronium atom

A detailed quantum-electrodynamic calculation of muon pair creation in laser-driven electron-positron collisions is presented. The colliding particles stem from a positronium atom exposed to a superintense laser wave of linear polarization, which allows for high luminosity. The threshold laser intensity of this high-energy reaction amounts to a few 10^22 W/cm^2 in the near-infrared frequency range. The muons produced form an ultrarelativistic, strongly collimated beam, which is explicable in terms of a classical simple-man's model. Our results indicate that the process can be observed at high positronium densities with the help of present-day laser technology.Comment: 4 pages, 3 figure

Fly ash-derived MCM-41 as a low-cost silica support for polyethyleneimine in post-combustion CO2 capture

The mesoporous silicate molecular sieve, MCM-41, has been synthesized from pulverized coal fly ash (PFA), where the silicate filtrate used is a by-product from hydrothermal zeolite production. Rice husk ash was also used for comparison but fusion with sodium hydroxide was used to prepare the silicate filtrate, along similar lines to earlier reports of using PFA as a precursor for MCM-41 synthesis. The MCM-41 samples are chemically and mineralogically similar to a commercially available sample, but with higher pore volumes dominated by mesopores (0.92–1.13 cf. 0.88 cm3 g−1). After polyethyleneimine (PEI) impregnation for CO2 capture, the ash derived MCM-41 samples displayed higher uptakes than the commercial sample with the maximum achievable PEI loading of 60 Wt.% PEI (dry basis) before particle agglomeration occurs, approximately 13 compared to 11 Wt.%, respectively, the latter being comparable to earlier reports in the literature. The PFA sample that displays the fastest kinetics to achieve 90% of the equilibrium uptake had the largest mesopore volume of 1.13 cm3 g−1. Given the PFA-derived MCM-41 uses a waste silicate solution for hydrothermal preparation and no prior preparation is needed, production costs are estimated to be considerable lower where silicate solutions need to be prepared by base treatment, even if ash is used, as for the RHA derived MCM-41 used here

Inflationary Cosmological Perturbations of Quantum-Mechanical Origin

This review article aims at presenting the theory of inflation. We first describe the background spacetime behavior during the slow-roll phase and analyze how inflation ends and the Universe reheats. Then, we present the theory of cosmological perturbations with special emphasis on their behavior during inflation. In particular, we discuss the quantum-mechanical nature of the fluctuations and show how the uncertainty principle fixes the amplitude of the perturbations. In a next step, we calculate the inflationary power spectra in the slow-roll approximation and compare these theoretical predictions to the recent high accuracy measurements of the Cosmic Microwave Background radiation (CMBR) anisotropy. We show how these data already constrain the underlying inflationary high energy physics. Finally, we conclude with some speculations about the trans-Planckian problem, arguing that this issue could allow us to open a window on physical phenomena which have never been probed so far.Comment: Review Article, 47 pages, 3 figures. Lectures given at the 40th Karpacz Winter School on Theoretical Physics (Poland, Feb. 2004), submitted to Lecture Notes in Physic

The Sachs-Wolfe Effect: Gauge Independence and a General Expression

In this paper we address two points concerning the Sachs-Wolfe effect: (i) the gauge independence of the observable temperature anisotropy, and (ii) a gauge-invariant expression of the effect considering the most general situation of hydrodynamic perturbations. The first result follows because the gauge transformation of the temperature fluctuation at the observation event only contributes to the isotropic temperature change which, in practice, is absorbed into the definition of the background temperature. Thus, we proceed without fixing the gauge condition, and express the Sachs-Wolfe effect using the gauge-invariant variables.Comment: 5 pages, closer to published versio

A gain-of-function TBX20 mutation causes congenital atrial septal defects, patent foramen ovale and cardiac valve defects

BACKGROUND: Ostium secundum atrial septal defects (ASDII) account for approximately 10% of all congenital heart defects (CHD) and mutations in cardiac transcription factors, including TBX20, were identified as an underlying cause for ASDII. However, very little is known about disease penetrance in families and functional consequences of inherited TBX20 mutations. METHODS: The coding region of TBX20 was directly sequenced in 170 ASDII patients. Functional consequences of one novel mutation were investigated by surface plasmon resonance, CD spectropolarymetry, fluorescence spectrophotometry, luciferase assay and chromatin immunoprecipitation. RESULTS: We found a novel mutation in a highly conserved residue in the T-box DNA-binding domain (I121M) segregating with CHD in a three generation kindred. Four mutation carriers revealed cardiac phenotypes in terms of cribriform ASDII, large patent foramen ovale or cardiac valve defects. Interestingly, tertiary hydrophobic interactions within the mutant TBX20 T-box were significantly altered leading to a more dynamic structure of the protein. Moreover, Tbx20-I121M resulted in a significantly enhanced transcriptional activity, which was further increased in the presence of co-transcription factors GATA4/5 and NKX2-5. Occupancy of DNA binding sites on target genes was also increased. CONCLUSIONS: We suggest that TBX20-I121M adopts a more fluid tertiary structure leading to enhanced interactions with cofactors and more stable transcriptional complexes on target DNA sequences. Our data, combined with that of others, suggest that human ASDII may be related to loss- as well as gain-of-function TBX20 mutations

Inhibition of Growth Factor-Mediated Tyrosine Phosphorylation in Vascular Smooth Muscle by PD 089828, a New Synthetic Protein Tyrosine Kinase Inhibitor

ABSTRACT PD 089828, a novel protein tyrosine kinase inhibitor of a new structural class, the 6-aryl-pyrido- [2,3-d]pyrimidines, was identified by screening a compound library with assays that measured protein tyrosine kinase activity. PD 089828 was found to inhibit human full-length fibroblast growth factor (FGF) receptor-1 (FGFR-1), platelet-derived growth factor (PDGF) receptor ␤ subunit (PDGFR-␤), Src nonreceptor tyrosine kinase (c-Src) and epidermal growth factor (EGF) receptor (EGFR) tyrosine kinases with half-maximal inhibitory potencies (IC 50 values) of 0.15 Ϯ 0.02 (n ϭ 4), 0.18 Ϯ 0.04 (n ϭ 3), 1.76 Ϯ 0.28 (n ϭ 4) and 5.47 Ϯ 0.78 (n ϭ 6) M, respectively. PD 089828 was further characterized as an ATP competitive inhibitor of the growth factor receptor tyrosine kinases (FGFR-1, PDGFR-␤ and EGFR) but a noncompetitive inhibitor of c-Src tyrosine kinase with respect to ATP. In addition, PD 089828 inhibited PDGF-and EGF-stimulated receptor autophosphorylation in vascular SMC (VSMC) and basic FGF-mediated tyrosine phosphorylation in A121 cells with IC 50 values similar to the potencies observed for inhibition of receptor tyrosine kinase activity. The inhibition of PDGF receptor autophosphorylation in VSMC by PD 089828 occurred rapidly, with maximal effects reached within 5 min of drug exposure. Inhibition after single exposure was long lasting but also rapidly reversible, occurring within 5 min after drug removal. The PDGF-induced association of downstream signaling proteins, including phosphoinositide-3-kinase (PI-3K), growth factor receptor binding protein-2 (GRB2), SH-2 domain and collagen like (Shc) and phospholipase C␥ (PLC␥), with VSMC PDGF receptors was also blocked as a result of the inhibition of PDGF-stimulated receptor autophosphorylation by PD 089828. PD 089828 also inhibited the PDGF-induced tyrosine phosphorylation of the 44-and 42-kDa mitogen-activated protein kinase isoforms. Moreover, the effects of PD 089828 were demonstrated in functional assays in which PDGF-stimulated DNA synthesis, PDGF-directed migration and serum-stimulated growth of VSMC were all inhibited to the same extent as PDGF receptor autophosphorylation (IC 50 ϭ 0.8, 4.5 and 1.8 M, respectively). These results highlight the biological characteristics of PD 089828 as a novel, broadly active protein tyrosine kinase inhibitor with long-lasting but reversible cellular effects. The potential therapeutic use of these broadly acting, nonselective inhibitors as antiproliferative and antimigratory agents could extend to such diseases as cancer, atherosclerosis and restenosis in which redundancies in growth-signaling pathways are known to exist