Carotenoid Content in Cherry Tomatoes Correlated to the Color Space Values L*, a*, b*: A Non-destructive Method of Estimation

Cherry tomatoes are rich sources of carotenoids. The carotenoids are known to be precursors of vitamin A and also act as an antioxidant. It is important to visually judge the tomato surface color for higher β carotene content since this is the major provitamin AA carotenoid. Estimation of carotenoids by HPLC (High Performance Liquid Chromatography) and spectrophotometric methods in tomatoes are very expensive and time consuming. Therefore, colorimeters can be used to describe the color and determine the carotenoid content in a relatively easy and inexpensive manner. The objective of this study was to determine, if the carotenoid content within cherry tomatoes measured by conventional method could correlate with colorimetric CIE (Commission International del’Eclairage) L*, a*, b* color space values. Strong correlations were found between color surface value a* and total carotenoids (0.82) and lycopene content (0.87). We also observed positive correlation for the b* color value with β carotene (0.86). The L* value was negatively correlated (-0.78) with an increase in carotenoids. These close associations between color space values L*, a*, b* and carotenoids will help the breeders to quickly screen large germplasm/ breeding lines in their breeding program for improvement in carotenoid content through this time saving, inexpensive and nondestructive method at fully ripe stage

ICP 7035 – A Sterility Mosaic Resistant Vegetable and Grain Purpose Pigeonpea Variety

ICP 7035 is a medium duration, non-determinate pigeonpea landrace collected in 1973 from Bedaghat (near Jabalpur), Madhya Pradesh, India. Plants mature in 170-200 days (in south-central regions of India) and, at this stage, reach an average height of 120-140 cm. Each plant produced around 100 pods and each pod contained 5 seeds, which are nutritionally rich and contain high percentages of digestible carbohydrates, vitamins and micronutrients. The large seeds (9-11 mm diameter) had purple seed coats and green cotyledons, and are suitable for consumption as vegetable. The fresh seed contains 8.6% protein, 12% fibre, and 45.7% carbohydrate and starch. The pinkish-purple colour of the pod and seed coat is due to high anthocyanin contents. While the normal sugar level in most pigeon pea cultivars is approximately 5%, the sugar content in ICP 7035 seeds is 8.8%. Decorticated dried split seeds measure 5-6 mm in diameter and 100 dried seeds weigh 19.2 g. The seed contains 19.6% protein, 27.4% dietary fibre, 33% starch and 67% carbohydrate, and has high amounts of copper, calcium, magnesium and phosphorous. Resistance to Pigeonpea sterility mosaic virus in ICP 7035 has a positive impact on yield as a result of negligible crop loss in endemic areas. In the absence of the disease, the yield of ICP 7035 is on a par with the yields of local cultivars. Recently, provisional approval was given for the release of this cultivar in SMD endemic areas of southern Karnataka

Gamma irradiation of platinum- and palladium-related deep levels in silicon

258-262Effect of 1.1 MeV gamma irradiation has been studied on Au/n Si:Pt and Au/n Si:Pd Schottky barrier diodes. A comparison of the results from these two types of structures illustrate the influence of device processing on the type of defects formed by subsequent irradiation. Before irradiation the energy levels for Pt and Pd in n-type silicon have been observed at Ec-0.28eV, Ec-0.52eV and Ec-0.22eV  respectively. After irradiation and annealing all the energy levels due to irradiation are disappeared except Ec-0.28eV in Au/n - Si: Pt and Ec-0.22eV in Au/n - Si: Pd Schottky diodes

Gamma irradiation of platinum- and palladium-related deep levels in silicon

Effect of 1.1 MeV gamma irradiation has been studied on Au/n- Si:Pt and Au/n-Si:Pd Schottky barrier diodes. A comparison of the results from these two types of structures illustrate the influence of device processing on the type of defects formed by subsequent irradiation. Before irradiation the energy levels for Pt and Pd in n-type silicon have been observed at E-c-0.28 eV, E-c-0.52 eV and E-c-0.22 eV respectively. After irradiation and annealing all the energy levels due to irradiation are disappeared except E-c-0.28 eV in Au/n-Si:Pt and E-c-0.22 eV in Au/n-Si:Pd Schottky diodes

DataSheet_1_Co-expression of stress-responsive regulatory genes, MuNAC4, MuWRKY3 and MuMYB96 associated with resistant-traits improves drought adaptation in transgenic groundnut (Arachis hypogaea l.) plants.doc

Groundnut, cultivated under rain-fed conditions is prone to yield losses due to intermittent drought stress. Drought tolerance is a complex phenomenon and multiple gene expression required to maintain the cellular tolerance. Transcription factors (TFs) regulate many functional genes involved in tolerance mechanisms. In this study, three stress-responsive regulatory TFs cloned from horse gram, (Macrotyloma uniflorum (Lam) Verdc.), MuMYB96, involved in cuticular wax biosynthesis; MuWRKY3, associated with anti-oxidant defense mechanism and MuNAC4, tangled with lateral root development were simultaneously expressed to enhance drought stress resistance in groundnut (Arachis hypogaea L.). The multigene transgenic groundnut lines showed reduced ROS production, membrane damage, and increased superoxide dismutase (SOD) and ascorbate peroxidase (APX) enzyme activity, evidencing improved antioxidative defense mechanisms under drought stress. Multigene transgenic plants showed lower proline content, increased soluble sugars, epicuticular wax content and higher relative water content suggesting higher maintenance of tissue water status compared to wildype and mock plants. The scanning electron microscopy (SEM) analysis showed a substantial increase in deposition of cuticular waxes and variation in stomatal number in multigene transgenic lines compared to wild type and mock plants. The multigene transgenic plants showed increased growth of lateral roots, chlorophyll content, and stay-green nature in drought stress compared to wild type and mock plants. Expression analysis of transgenes, MuMYB96, MuWRKY3, and MuNAC4 and their downstream target genes, KCS6, KCR1, APX3, CSD1, LBD16 and DBP using qRT-PCR showed a two- to four-fold increase in transcript levels in multigene transgenic groundnut plants over wild type and mock plants under drought stress. Our study demonstrate that introducing multiple genes with simultaneous expression of genes is a viable option to improve stress tolerance and productivity under drought stress.</p

DataSheet_2_Co-expression of stress-responsive regulatory genes, MuNAC4, MuWRKY3 and MuMYB96 associated with resistant-traits improves drought adaptation in transgenic groundnut (Arachis hypogaea l.) plants.doc

Groundnut, cultivated under rain-fed conditions is prone to yield losses due to intermittent drought stress. Drought tolerance is a complex phenomenon and multiple gene expression required to maintain the cellular tolerance. Transcription factors (TFs) regulate many functional genes involved in tolerance mechanisms. In this study, three stress-responsive regulatory TFs cloned from horse gram, (Macrotyloma uniflorum (Lam) Verdc.), MuMYB96, involved in cuticular wax biosynthesis; MuWRKY3, associated with anti-oxidant defense mechanism and MuNAC4, tangled with lateral root development were simultaneously expressed to enhance drought stress resistance in groundnut (Arachis hypogaea L.). The multigene transgenic groundnut lines showed reduced ROS production, membrane damage, and increased superoxide dismutase (SOD) and ascorbate peroxidase (APX) enzyme activity, evidencing improved antioxidative defense mechanisms under drought stress. Multigene transgenic plants showed lower proline content, increased soluble sugars, epicuticular wax content and higher relative water content suggesting higher maintenance of tissue water status compared to wildype and mock plants. The scanning electron microscopy (SEM) analysis showed a substantial increase in deposition of cuticular waxes and variation in stomatal number in multigene transgenic lines compared to wild type and mock plants. The multigene transgenic plants showed increased growth of lateral roots, chlorophyll content, and stay-green nature in drought stress compared to wild type and mock plants. Expression analysis of transgenes, MuMYB96, MuWRKY3, and MuNAC4 and their downstream target genes, KCS6, KCR1, APX3, CSD1, LBD16 and DBP using qRT-PCR showed a two- to four-fold increase in transcript levels in multigene transgenic groundnut plants over wild type and mock plants under drought stress. Our study demonstrate that introducing multiple genes with simultaneous expression of genes is a viable option to improve stress tolerance and productivity under drought stress.</p