Sequencing Closterium moniliferum: Future prospects in nuclear waste disposal

AbstractGenome sequencing can play a vital role in health and several other domains such as in nuclear outflow related environmental issues. The power of information derived out of sequencing has been used in the field of health care, evolutionary studies and for better understanding of the biological framework of life. Through the recent advancements in sequencing studies, now the researchers are aiming to use its power in non conventional areas. Here we have discussed on the importance of sequencing the Closterium moniliferum genome which will prove to be a future endeavour in nuclear cleanup and radioactive waste disposal

Fetal growth and its correlation with level of glycemic control in pregnancy with diabetes: an observational study in tertiary care centre of North India

Background: Diabetes in pregnancy is a known risk factor for macrosomia and intensive glycemic control is a well-known strategy to prevent this macrosomia. However, does this tight glycemic control is actually beneficial or is it one of the reasons for small for gestational age babies in these women? Is a clinical enigma. We planned this study to see effects of glycemic control on fetal weight and to answer if tight control is always better.Methods: This prospective observational study was conducted in the department of obstetrics and gynaecology in a tertiary care centre (King George medical university) over a period of one year (June 2017-June 2018). All pregnant women with GDM and pre-gestational diabetes with singleton pregnancy were registered in the study after proper consent, followed up for glycemic control, fetal weight. Antepartum risk factors and complications of diabetes were also noted in these women.Results: Total 88 patients included in the study. Five with pre-gestational diabetes, 83 with GDM. Small for gestational age neonates were seen in 54.1% cases, large for gestational age were seen in 2 cases and rest of neonates were appropriate for gestational age. 89.4% had good glycemic control, 7% had over-zealous glycemic control and 3.5% had under-controlled sugars.Conclusions: The results in the study strongly supported the efficacy of good glycemic control in prevention of LGA/macrosomia. However, optimal glycemic control in third trimester does not guarantee appropriate weight of fetus as incidence of SGA/FGR neonates was fairly high (53.9%) even in good glycemic control group

Scar endometriosis: pre-operative diagnosis by fine needle aspiration cytology

Scar endometriosis is a rare entity commonly observed after obstetrical and gynaecological procedures. The diagnosis is often delayed due to the non-specific nature of symptoms. Detailed clinical history of cyclical pain, location in proximity to a surgical scar and a suspicion of this rare entity in women of childbearing age are key to preoperative diagnosis. This is a case of a patient who presented with a troublesome scar after Caesarean section. On Fine needle aspiration cytology (FNAC) a diagnosis of scar endometriosis was provided which was further confirmed on histopathology. Herein we discuss the cytomorphological features of this rare entity and also emphasize the importance of its diagnosis on FNAC which is a rapid and cost-effective method

Clinical Pharmacology & Therapeutic uses of Diuretic Agents: A Review

The osmolarity of both blood and urine will increase if the kidneys are unable to eliminate excess water and electrolytes. NPS can be treated by restricting salt intake and using diuretics. Loop diuretics. Salt and water are flushed out of the body by diuretics. Medications have a sodium-lowering effect. arteries parched with salt BP-lowering. Albumin and diuretics both have the effect of decreasing sensitivity. Furosemide inhibits albumin. Fail-safe. Albuminuria, coagulopathy, dyslipidemia, edema. Diuretics are used to treat both edema and non-edema. Diuretics are used to treat heart failure, high blood pressure, and ascites (and other disorders when applicable). Treatment of heart failure, side effects, off-label usage, dose, pharmacokinetics, monitoring, and interactions are all topics that will be covered in this article. Thiazide diuretics that are authorized by the FDA impede between 3 and 5 percent of nephron DCT sodium reabsorption. Thiazides cause a person to urinate more often. Nephron trafficking is slowed down when diuretics are used. Furosemide, bumetanide, torsemide inhibit Na-K-2Cl (SLC12A1). Chloride-binding proteins can only be bound to by anions. Perform the initial dose once again. Bronchodilators that open up the airways. The administrator of the test will be able to tell if your airways constrict (spirometry). Chemicals that because inflammation weaken smooth muscle. Drops of 15 percent in the forced expiratory volume in one second (FEV1) suggest airway hyperreactivity and inflammation

Applicability of Native L-Arginase produced by Streptomyces plicatus KAR73 as Antineoplastic Agent

841-849Most of the cancer cells require high quantity of arginine for sustaining their fast-metabolic rates. Limiting supply of arginine to cancer cells using arginase may prove to be of great therapeutic value. The arginase produced by a micro organism isolated from soil has been used in industrial production of ornithine, however its use in anticancer activities is scarcely studied. This study optimized soybean meal supplemented basal media for Arginase production. Arginase was purified using ammonium sulphate precipitation, sephadex G-100 column chromatography and DEAE chromatography achieving 79.2% purification fold and 24.26% yield. It had 23 KD molecular weight as determined using Native PAGE and was active at considerably wide pH range of 6–10 and temperature 30–50℃. Whereas the maximum arginase activity was noticed with Mn²+ ions followed by polyvinyl pyrrolidine (PVP) at 70 mM substrate concentration, the maximum inhibition of activity was caused by CuC. Streptomyces plicatus KAR 73 produced arginase on mouse mammary cell line (CID 9) was not inhibited by the arginase upto 6.5 U/mL. Significant (p50 5.2 U/mL. The ornithine has been produced earlier with Mycoplasma and Clostridium by other researchers but production of native arginase from Streptomyces specifically for anticancer activities has not yet been reported. The present study infers that Arginase produced from native Streptomyces has shown promising results thereby enabling feasibility assessment towards cost effective industrial production of arginase

Applicability of Native L-Arginase produced by Streptomyces plicatus KAR73 as Antineoplastic Agent

Most of the cancer cells require high quantity of arginine for sustaining their fast-metabolic rates. Limiting supply of arginine to cancer cells using arginase may prove to be of great therapeutic value. The arginase produced by a micro organism isolated from soil has been used in industrial production of ornithine, however its use in anticancer activities is scarcely studied. This study optimized soybean meal supplemented basal media for Arginase production. Arginase was purified using ammonium sulphate precipitation, sephadex G-100 column chromatography and DEAE chromatography achieving 79.2% purification fold and 24.26% yield. It had 23 KD molecular weight as determined using Native PAGE and was active at considerably wide pH range of 6–10 and temperature 30–50℃. Whereas the maximum arginase activity was noticed with Mn²+ ions followed by polyvinyl pyrrolidine (PVP) at 70 mM substrate concentration, the maximum inhibition of activity was caused by CuC. Streptomyces plicatus KAR 73 produced arginase on mouse mammary cell line (CID 9) was not inhibited by the arginase upto 6.5 U/mL. Significant (p< 0.001) inhibition in Mouse mammary tumor (C1271) cell lines was observed with IC50 5.2 U/mL. The ornithine has been produced earlier with Mycoplasma and Clostridium by other researchers but production of native arginase from Streptomyces specifically for anticancer activities has not yet been reported. The present study infers that Arginase produced from native Streptomyces has shown promising results thereby enabling feasibility assessment towards cost effective industrial production of arginase

Emotion regulation difficulties in alexithymia and mental health

Alexithymia, characterized by difficulty in identifying and describing feelings and a deficit in the cognitive modulation of emotions, has been linked with health related problems. Indirect empirical evidences suggest the possibility that alexithymia may potentially also relate with emotion regulation difficulties. However, the exact nature of the relationship between the two constructs has not been fully explored. The relationship between the two constructs may reflect the overlap of the content domains or dependence of one (e.g., emotion regulation difficulties) on the other (e.g., alexithymia). Given the link between the two constructs, it is also likely that alexithymics’ health related difficulties may result from their impaired ability to regulate emotions. The present study tested the above theoretical propositions on 27 alexithymic and 26 non-alexithymic participants who were assessed on self-report measures of emotion regulation difficulties and general mental health. The findings revealed that compared to non-alexithymics, the alexithymics showed greater emotion regulation difficulties and such affective difficulties differentiated the two groups with a very high accuracy. Further, the observation of two orthogonal components (emotion regulation difficulties and alexithymia) with a minimum overlap suggests that these constructs represent two independent domains of behavior. Findings also revealed that alexithymics reported more health related problems as compared to non-alexithymics and the health problems of alexithymics are likely to be an outcome of their emotion regulation difficulties (indicated by a non-significant health difference after controlling for emotion regulation difficulties). The findings imply that alexithymia is associated with emotion regulation difficulties and such difficulties largely contribute to health related problems

Emotion regulation difficulties in alexithymia and mental health

Alexithymia, characterized by difficulty in identifying and describing feelings and a deficit in the cognitive modulation of emotions, has been linked with health related problems. Indirect empirical evidences suggest the possibility that alexithymia may potentially also relate with emotion regulation difficulties. However, the exact nature of the relationship between the two constructs has not been fully explored. The relationship between the two constructs may reflect the overlap of the content domains or dependence of one (e.g., emotion regulation difficulties) on the other (e.g., alexithymia). Given the link between the two constructs, it is also likely that alexithymics’ health related difficulties may result from their impaired ability to regulate emotions. The present study tested the above theoretical propositions on 27 alexithymic and 26 non-alexithymic participants who were assessed on self-report measures of emotion regulation difficulties and general mental health. The findings revealed that compared to non-alexithymics, the alexithymics showed greater emotion regulation difficulties and such affective difficulties differentiated the two groups with a very high accuracy. Further, the observation of two orthogonal components (emotion regulation difficulties and alexithymia) with a minimum overlap suggests that these constructs represent two independent domains of behavior. Findings also revealed that alexithymics reported more health related problems as compared to non-alexithymics and the health problems of alexithymics are likely to be an outcome of their emotion regulation difficulties (indicated by a non-significant health difference after controlling for emotion regulation difficulties). The findings imply that alexithymia is associated with emotion regulation difficulties and such difficulties largely contribute to health related problems

Analysis of Wide Modified Rankin Score Dataset using Markov Chain Monte Carlo Simulation

Brain hemorrhage and strokes are serious medical conditions that can have devastating effects on a person's overall well-being and are influenced by several factors. We often encounter such scenarios specially in medical field where a single variable is associated with several other features. Visualizing such datasets with a higher number of features poses a challenge due to their complexity. Additionally, the presence of a strong correlation structure among the features makes it hard to determine the impactful variables with the usual statistical procedure. The present paper deals with analysing real life wide Modified Rankin Score dataset within a Bayesian framework using a logistic regression model by employing Markov chain Monte Carlo simulation. Latterly, multiple covariates in the model are subject to testing against zero in order to simplify the model by utilizing a model comparison tool based on Bayes Information Criterion