Assessing a sustainable manufacturing route to lapatinib

A synthetic route to an anti-cancer drug, lapatinib, was devised to support the development of a sustainable manufacturing process in South Africa. Quantitative metrics were employed to evaluate the sustainability of the key steps of the reaction

Molecular DNA identity of the mouflon of Cyprus (Ovis orientalis ophion, Bovidae): Near Eastern origin and divergence from Western Mediterranean conspecific populations

The mouflon population of Cyprus (Ovis orientalis ophion) comprises historically preserved feral descendants of sheep domesticated during the Neolithic. We determined genetic identity of this taxon in order to elucidate its systematic placement and enforce its protection. We used 12 loci of microsatellite DNA to infer genetic relationships between the Cypriot mouflon and either long-time isolated (Corsica, Sardinia) or recently introduced (central Italy) European mouflons (O. o. musimon). We also sequenced the mitochondrial DNA (mtDNA) Cytochrome-b gene to infer the origin of the Cypriot mouflon including many National Centre for Biotechnology Information (NCBI) entries of European and Near Eastern conspecifics. Microsatellites disclosed net divergence between Western Mediterranean and Cypriot mouflon. The latter was included in the highly heterogeneous Near Eastern O. orientalis mtDNA group, Iran representing the most credited region as the source for its ancient introduction to Cyprus. Both international and national legislation protect the mouflon of Cyprus as a wild taxon (O. o. ophion). However, the IUCN Red List of Threatened Species and NCBI include the Cypriot mouflon as subspecies of its respective domestic species, the sheep (O. aries). Unfortunately, people charged with crime against protected mouflon may benefit from such taxonomic inconsistency between legislation and databases, as the latter can frustrate molecular DNA forensic outcomes. Until a definitive light can be shed on Near Eastern O. orientalis systematics, we suggest that the Cypriot mouflon should be unvaryingly referred to as O. o. ophion in order not to impair conservation in the country where it resides

Portuguese validation of the Internet Addiction Test: An empirical study

Abstract Background and aims Research into Internet addiction (IA) has increased greatly over the last decade. Despite its various definitions and general lack of consensus regarding its conceptualisation amongst researchers, instruments for measuring this phenomenon have proliferated in a number of countries. There has been little research on IA in Portugal and this may be partly due to the absence of standardised measurement tools for assessing IA. Methods This study attempted to address this issue by adapting a Portuguese version of the Internet Addiction Test (IAT) via a translation-back translation process and Confirmatory Factor Analysis in a sample of 593 Portuguese students that completed a Portuguese version of the IAT along with questions related to socio-demographic variables. Results The findings suggested that the IAT appears to be a valid and reliable instrument for measuring IA among Portuguese young adults as demonstrated by its satisfactory psychometric properties. However, the present findings also suggest the need to reword and update some of the IAT's items. Prevalence of IA found in the sample was 1.2% and is discussed alongside findings relating to socio-demographic correlates. Limitations and implications of the present study are also discussed. Conclusions The present study calls for a reflection of the IAT while also contributing to a better understanding of the basic aspects of IA in the Portuguese community since many health practitioners are starting to realise that Internet use may pose a risk for some individuals

Vertebrate tracks in Late Pleistocene–Early Holocene (?) carbonate aeolianites, Paphos, Cyprus

In 2005, numerous vertebrate tracks were discovered in carbonate aeolianites in and around the town of Paphos, in the south western part of Cyprus. The main track-bearing exposure is located in a protected archaeological site near the Agia Solomoni Church in side the city of Paphos, where cross-sec tions through tracks are abundant in vertical exposures of the aeolianite along Apostolou Pavlou Avenue. Some exposures show as many as 10 tracks per m2 of vertical exposure. Several additional tracks were found in the extensive subterranean tomb complex, the Tombs of the Kings, just outside Paphos. The aeolian deposit was formed when westerly to southwesterly windsdrove fine- to medium-grained calcareous sand onshore from the beach. This generated low coastal dunes, represented by 1–2-m-thick, cross-bedded sets made up of grainflow and wind-ripple strata, and sand sheets composed entirely of wind-ripple strata. The sediment does not yet have an absolute date, but is considered to be of Late Pleistocene to Early Holocene age, as are many other coastal aeolianites in the Mediterranean area. The Late Pleistocene endemic fauna in Cyprus was limited to the dwarf hippopotamus Phanourios minor Desmarest, 1822, the dwarf elephant Elephas cypriotes Bate, 1902, a small carnivore Genetta plesictoides Bate, 1903, and (possibly) humans. The exposed tracks are 5–15 cm in diameter, with a few tracks up to 23 cm in size. This range of size correlates well with the estimated foot size of dwarf hippopotami and dwarf elephants. This low-diversity, endemic is land fauna provides a unique opportunity to correlate tracks with trackmakers

Rapid formation of 2-lithio-1-(triphenylmethyl)imidazole and substitution reactions in flow

The functionalisation of imidazoles is a necessary step in the formation of many active pharmaceutical intermediates. Herein, we report a flow chemistry approach for the rapid and efficient formation of 2-lithio-1-(triphenylmethyl)imidazole at ambient temperature and its reaction with a range of electrophiles, achieving modest to high yields (40–94%) in short reaction times (<1 min). The method is amenable to the scale-up of this highly reactive lithio-imidazole intermediate

Friedreich's ataxia phenotype not linked to chromosome 9 and associated with selective autosomal recessive vitamin E deficiency in two inbred Tunisian families

Friedreich's ataxia (FA) is an autosomal recessive neurodegenerative disorder, the disease locus (FRDA) of which has been assigned to 9q13-q21.1 by genetic linkage analysis in affected families. We report two large inbred Tunisian families with FA manifestations that did not show the expected linkage. The disease locus could be excluded from a large (12 cMo) region around FRDA. This is the first report providing evidence for nonallelic genetic heterogeneity for the FA clinical phenotype. On subsequent analysis, all patients had very low levels of serum vitamin E whereas the parents and healthy sibs had normal vitamin E levels. This establishes that the selective vitamin E deficiency with normal fat absorption is an autosomal recessive trait, which is associated in the two families reported here with the FA phenotype