Vegetation above a feral pig barrier fence in rain forests of Kilauea's East Rift, Hawaii Volcanoes National Park

Reports were scanned in black and white at a resolution of 600 dots per inch and were converted to text using Adobe Paper Capture Plug-in.Immediately after the 1993 construction of a barrier fence to block the movements of feral pigs in forests of Kilauea's East Rift within Hawaii Volcanoes National Park (HAVO), a systematic framework of transects and plots was established for collection of baseline vegetation data upslope of the fence. Distribution and estimated abundance of the most invasive alien plant species were determined. The most widespread alien grass species was Hilo grass (Paspalum conjugatum); although it typically had low estimated cover values, this grass was almost ubiquitous. The most common invasive alien tree species was strawberry guava (Psidium cattleianum); it was found along transects in the western half of the study area, where its cover was estimated as 5-25% or 25-50%. Firetree (Myrica faya) occurred less frequently and had lower cover values than strawberry guava. Two other invasive woody species were found at low frequency or in limited areas; yellow Himalayan raspberry (Rubus ellipticus) was restricted to the slopes of Kane Nui o Hamo, and cane tibouchina (Tibouchina herbacea) occurred infrequently at widely scattered localities. Alien plant frequencies from the current survey were compared with those from a previous plant survey in 1988. A third of the alien plant species along transects, including firetree, yellow raspberry, and strawberry guava, had very similar frequencies on both surveys. Frequency of Hilo grass and scaly swordfern (Nephrolepis multiflora) increased greatly in the study area between the two surveys. Some of the observed changes in vegetation may have been influenced by recent disturbance to the forests by cinder deposits from Pu'u '0'o, in an earlier phase of the current eruption. The locations of rare native plants were mapped along transects, and numbers were compared with those from the previous survey of 1988. The endangered pendent kihi fern (Adenophorus periens) was not relocated on Park transects; this species may have disappeared from the slopes of Kane Nui o Hamo in the last five years. Koli'i (Trematolobelia grandifolia), a "species of concern" has persisted on Kane Nui o Hamo, and its current size class structure indicates a stable population. The 12 rare plant species that were observed on East Rift transects were concentrated in several sites, including Kane Nui o Hamo, forests south and west of Napau crater, relatively open forest southeast of the 1840 flow, and the southwest corner of the study area near the Naulu Trail. Frequencies of 'oha (Clermontia spp.), indicator species for pig damage in Hawai'i, were relatively high overall in the study area, although the impact of pig predation was indicated by the paucity of terrestrial plants and a low freqeuncy of large Clermontia. Remonitoring a subset of transects after 1.5 years revealed that terrestrial Clermontia declined in frequency, while epiphytic plants increased over the same period. Tree fern density in the study area was 38/100m2, and the trunk height class of 1-2 m was well represented in East Rift forests. A higher density of tree ferns was observed in the western half of the study area, primarily due to the greater number of tree ferns >1m. There were fewer tree ferns on the lower halves of three main transects than were found on the upper reaches, farthest away from the barrier fence and upslope from uluhe-dominated forest. 'Olapa (Cheirodendron trigynum) appeared to be an inconsistent indicator species for pig activity. This important rain forest tree was maintaining a stable population in East Rift forests, despite the long-term presence of feral pigs there. Differences in 'olapa density were noted in the western versus the eastern, unprotected part of the study area, where lower numbers of terrestrial 'olapa saplings were seen. When compared with the upper transect reaches, fewer 'olapa saplings were found along the lower portions of the three main transects, in the area near the open end of the barrier fence. Vegetation cover and woody plant density of recently pig-disturbed sites were examined in 1994 using 39 vegetation plots, and a subset of 26 plots was remonitored 1.5-2 years later. The vegetation of disturbed East Rift forests was found to be poor in native woody plant species, and ground cover was very sparse. In the interval between monitoring, the cover of two alien species (Hilo grass and scaly swordfern) increased dramatically. Early succession indicates that these two plants will become dominant components of ground cover in pig-disturbed areas. Little change was noted in native woody plant density overall, but several native species, including tree ferns, displayed increases over the 1.5 year monitoring period. Feral pig density, estimated from frequency of pig activity, was low in the East Rift study area (averaging 1.9 pig/km2), even before systematic control efforts began. Quarterly pig activity monitoring revealed an increase in activity along Park transects until the end of 1993, followed by a steady decline to 2.4 pig/km2 by the end of the study in January 1996. Data from this study suggest that the park's snaring efforts reduced the feral pig population in the most protected, interior part of the forest upslope of the barrier fence within two years of the project initiation. Success in lowering pig density was not observed on transects at or beyond the open terminus of the barrier fence, indicating that pig ingress continued throughout the study. The current survey may only be considered a baseline study of vegetation and pig activity in managed East Rift forests. Future remonitoring may be needed to evaulate the success of this management effort in promoting long-term recovery of native vegetation.National Park Service Cooperative Agreement 8010 2 900

Career pathways and professional skills of postgraduate students from a dental research-intensive programme

With current global trends in postgraduate education, graduate programmes must make evidence-based improvements to offer the best programme that aligns with student needs and prepare them for their future career prospects. The aim of this cross-sectional study was to investigate the postgraduation career pathways of MSc and PhD students who graduated within the past 15 years from the McGill University Postgraduate Dental Research Program.An online questionnaire, composed of 10 closed-ended format items, was used that covered domains such as student profile, career profile, postgraduate skill development, job search experience and satisfaction. Descriptive statistics and interpretative qualitative analysis were used to evaluate student feedback.Sixty-six students responded to the online survey, out of which sixty-two students completed the survey (61% participation rate). The majority of the graduate students, 67% (n = 44), obtained MSc degree in Dental Sciences. Overall, our results showed that most graduates started careers in academia in their original field of study and were satisfied with their income. Most graduates reported "critical and creative thinking" to be the strongest acquired skills during their postgraduate training and identified fierce competition for their position of interest as the main challenge after graduation.Our results showed that graduates in dental research appeared to be overall satisfied with their careers after postgraduate research training, both in terms of scope of practice and income. However, strong competition in obtaining the position of their interest seemed to be the main obstacle after graduation.</div

An assessment of hydrocarbon species in the methanol-to-hydrocarbon reaction over a ZSM-5 catalyst.

A ZSM-5 catalyst is examined in relation to the methanol-to-hydrocarbon (MTH) reaction as a function of reaction temperature and time-on-stream. The reaction profile is characterised using in-line mass spectrometry. Furthermore, the material contained within a catch-pot downstream from the reactor is analysed using gas chromatography-mass spectrometry. For a fixed methanol feed, reaction conditions are selected to define various stages of the reaction coordinate: (i) initial methanol adsorption at a sub-optimum reaction temperature (1 h at 200 °C); (ii) initial stages of reaction at an optimised reaction temperature (1 h at 350 °C); (iii) steady-state operation at an optimised reaction temperature (3 days at 350 °C); and (iv) accelerated ageing (3 days at 400 °C). Post-reaction, the catalyst samples are analysed ex situ by a combination of temperature-programmed oxidation (TPO) and spectroscopically by electron paramagnetic resonance (EPR), diffuse-reflectance infrared and inelastic neutron scattering (INS) spectroscopies. The TPO measurements provide an indication of the degree of 'coking' experienced by each sample. The EPR measurements detect aromatic radical cations. The IR and INS measurements reveal the presence of retained hydrocarbonaceous species, the nature of which are discussed in terms of the well-developed 'hydrocarbon pool' mechanism. This combination of experimental evidence, uniquely applied to this reaction system, establishes the importance of retained hydrocarbonaceous species in effecting the product distribution of this economically relevant reaction system

Transkingdom Analysis of the Female Reproductive Tract Reveals Bacteriophages form Communities

The female reproductive tract (FRT) microbiome plays a vital role in maintaining vaginal health. Viruses are key regulators of other microbial ecosystems, but little is known about how the FRT viruses (virome), particularly bacteriophages that comprise the phageome, impact FRT health and dysbiosis. We hypothesize that bacterial vaginosis (BV) is associated with altered FRT phageome diversity, transkingdom interplay, and bacteriophage discriminate taxa. Here, we conducted a retrospective, longitudinal analysis of vaginal swabs collected from 54 BV-positive and 46 BV-negative South African women. Bacteriome analysis revealed samples clustered into five distinct bacterial community groups (CGs), and further, bacterial alpha diversity was significantly associated with BV. Virome analysis on a subset of baseline samples showed FRT bacteriophages clustering into novel viral state types (VSTs), a viral community clustering system based on virome composition and abundance. Distinct BV bacteriophage signatures included increased alpha diversity along with discriminant Bacillus, Burkholderia, and Escherichia bacteriophages. Bacteriophage-bacteria transkingdom associations were also identified between Bacillus and Burkholderia viruses and BV-associated bacteria, providing key insights for future studies elucidating the transkingdom interactions driving BV-associated microbiome perturbations. In this cohort, bacteriophage-bacterial associations suggest complex interactions, which may play a role in the establishment and maintenance of BV

Studying black holes on horizon scales with space-VLBI

The Event Horizon Telescope (EHT) recently produced the first horizon-scale image of a supermassive black hole. Expanding the array to include a 3-meter space telescope operating at >200 GHz enables mass measurements of many black holes, movies of black hole accretion flows, and new tests of general relativity that are impossible from the ground

Application of Inelastic Neutron Scattering to the Methanol-to-Gasoline Reaction Over a ZSM-5 Catalyst

Inelastic neutron scattering (INS) is used to investigate a ZSM-5 catalyst that has been exposed to methanol vapour at elevated temperature. In-line mass spectrometric analysis of the catalyst exit stream confirms methanol-to-gasoline chemistry, whilst ex situ INS measurements detect hydrocarbon species formed in/on the catalyst during methanol conversion. These preliminary studies demonstrate the capability of INS to complement infrared spectroscopic characterisation of the hydrocarbon pool present in/on ZSM-5 during the MTG reaction

Pest population dynamics are related to a continental overwintering gradient

Overwintering success is an important determinant of arthropod populations that must be considered as climate change continues to influence the spatiotemporal population dynamics of agricultural pests. Using a long-term monitoring database and biologically relevant overwintering zones, we modeled the annual and seasonal population dynamics of a common pest, Helicoverpa zea (Boddie), based on three overwintering suitability zones throughout North America using four decades of soil temperatures: the southern range (able to persist through winter), transitional zone (uncertain overwintering survivorship), and northern limits (unable to survive winter). Our model indicates H. zea population dynamics are hierarchically structured with continental-level effects that are partitioned into three geographic zones. Seasonal populations were initially detected in the southern range, where they experienced multiple large population peaks. All three zones experienced a final peak between late July (southern range) and mid-August to mid-September (transitional zone and northern limits). The southern range expanded by 3% since 1981 and is projected to increase by twofold by 2099 but the areas of other zones are expected to decrease in the future. These changes suggest larger populations may persist at higher latitudes in the future due to reduced low-temperature lethal events during winter. Because H. zea is a highly migratory pest, predicting when populations accumulate in one region can inform synchronous or lagged population development in other regions. We show the value of combining long-term datasets, remotely sensed data, and laboratory findings to inform forecasting of insect pests

Premature Osteoblast Clustering by Enamel Matrix Proteins Induces Osteoblast Differentiation through Up-Regulation of Connexin 43 and N-Cadherin

In recent years, enamel matrix derivative (EMD) has garnered much interest in the dental field for its apparent bioactivity that stimulates regeneration of periodontal tissues including periodontal ligament, cementum and alveolar bone. Despite its widespread use, the underlying cellular mechanisms remain unclear and an understanding of its biological interactions could identify new strategies for tissue engineering. Previous in vitro research has demonstrated that EMD promotes premature osteoblast clustering at early time points. The aim of the present study was to evaluate the influence of cell clustering on vital osteoblast cell-cell communication and adhesion molecules, connexin 43 (cx43) and N-cadherin (N-cad) as assessed by immunofluorescence imaging, real-time PCR and Western blot analysis. In addition, differentiation markers of osteoblasts were quantified using alkaline phosphatase, osteocalcin and von Kossa staining. EMD significantly increased the expression of connexin 43 and N-cadherin at early time points ranging from 2 to 5 days. Protein expression was localized to cell membranes when compared to control groups. Alkaline phosphatase activity was also significantly increased on EMD-coated samples at 3, 5 and 7 days post seeding. Interestingly, higher activity was localized to cell cluster regions. There was a 3 fold increase in osteocalcin and bone sialoprotein mRNA levels for osteoblasts cultured on EMD-coated culture dishes. Moreover, EMD significantly increased extracellular mineral deposition in cell clusters as assessed through von Kossa staining at 5, 7, 10 and 14 days post seeding. We conclude that EMD up-regulates the expression of vital osteoblast cell-cell communication and adhesion molecules, which enhances the differentiation and mineralization activity of osteoblasts. These findings provide further support for the clinical evidence that EMD increases the speed and quality of new bone formation in vivo

Inactivation of DltA Modulates Virulence Factor Expression in Streptococcus pyogenes

D-alanylated lipoteichoic acid is a virtually ubiquitous component of gram-positive cell walls. Mutations in the dltABCD operon of numerous species exhibit pleiotropic effects, including reduced virulence, which has been attributed to increased binding of cationic antimicrobial peptides to the more negatively charged cell surface. In this study, we have further investigated the effects that mutating dltA has on virulence factor expression in Streptococcus pyogenes.Isogenic Delta dltA mutants had previously been created in two distinct M1T1 isolates of S. pyogenes. Immunoblots, flow cytometry, and immunofluorescence were used to quantitate M protein levels in these strains, as well as to assess their ability to bind complement. Bacteria were tested for their ability to interact with human PMN and to grow in whole human blood. Message levels for emm, sic, and various regulatory elements were assessed by quantitative RT-PCR. Cell walls of Delta dltA mutants contained much less M protein than cell walls of parent strains and this correlated with reduced levels of emm transcripts, increased deposition of complement, increased association of bacteria with polymorphonuclear leukocytes, and reduced bacterial growth in whole human blood. Transcription of at least one other gene of the mga regulon, sic, which encodes a protein that inactivates antimicrobial peptides, was also dramatically reduced in Delta dltA mutants. Concomitantly, ccpA and rofA were unaffected, while rgg and arcA were up-regulated.This study has identified a novel mechanism for the reduced virulence of dltA mutants of Streptococcus pyogenes in which gene regulatory networks somehow sense and respond to the loss of DltA and lack of D-alanine esterification of lipoteichoic acid. The mechanism remains to be determined, but the data indicate that the status of D-alanine-lipoteichoic acid can significantly influence the expression of at least some streptococcal virulence factors and provide further impetus to targeting the dlt operon of gram-positive pathogens in the search for novel antimicrobial compounds