46 research outputs found

    CHANNA STRIATUS TNFR-1: MOLECULAR CLONING, CHARACTERIZATION AND GENE EXPRESSION

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    Selective Colorimetric Detection of Nitrite in Water using Chitosan Stabilized Gold Nanoparticles Decorated Reduced Graphene oxide

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    © 2017 The Author(s). Excess nitrite (NO 2 - ) concentrations in water supplies is considered detrimental to the environment and human health, and is associated with incidence of stomach cancer. In this work, the authors describe a nitrite detection system based on the synthesis of gold nanoparticles (AuNPs) on reduced graphene oxide (rGO) using an aqueous solution of chitosan and succinic acid. The AuNPs-rGO nanocomposite was confirmed by different physicochemical characterization methods including transmission electron microscopy, elemental analysis, X-ray diffraction, UV-visible (UV-vis) and Fourier transform infrared spectroscopy. The AuNPs-rGO nanocomposite was applicable to the sensitive and selective detection of NO 2 - with increasing concentrations quantifiable by UV-vis spectroscopy and obvious to the naked eye. The color of the AuNPs-rGO nanocomposite changes from wine red to purple with the addition of different concertation of NO 2 - . Therefore, nitrite ion concentrations can be quantitatively detected using AuNPs-rGO sensor with UV-vis spectroscopy and estimated with the naked eye. The sensor is able to detect NO 2 - in a linear response ranging from 1 to 20 μM with a detection limit of 0.1 μM by spectrophotometric method. The as-prepared AuNPs-rGO nanocomposite shows appropriate selectivity towards NO 2 - in the presence of potentially interfering metal anions

    AI-Enabled Condition Monitoring Framework for Outdoor Mobile Robots Using 3D LiDAR Sensor

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    An automated condition monitoring (CM) framework is essential for outdoor mobile robots to trigger prompt maintenance and corrective actions based on the level of system deterioration and outdoor uneven terrain feature states. Vibration indicates system failures and terrain abnormalities in mobile robots; hence, five vibration threshold classes for CM in outdoor mobile robots were identified, considering both vibration source system deterioration and uneven terrain. This study proposes a novel CM approach for outdoor mobile robots using a 3D LiDAR, employed here instead of its usual use as a navigation sensor, by developing an algorithm to extract the vibration-indicated data based on the point cloud, assuring low computational costs without losing vibration characteristics. The algorithm computes cuboids for two prominent clusters in every point cloud frame and sets motion points at the corners and centroid of the cuboid. The three-dimensional vector displacement of these points over consecutive point cloud frames, which corresponds to the vibration-affected clusters, are compiled as vibration indication data for each threshold class. A simply structured 1D Convolutional Neural Network (1D CNN)-based vibration threshold prediction model is proposed for fast, accurate, and real-time application. Finally, a threshold class mapping framework is developed which fuses the predicted threshold classes on the 3D occupancy map of the workspace, generating a 3D CbM map in real time, fostering a Condition-based Maintenance (CbM) strategy. The offline evaluation test results show an average accuracy of vibration threshold classes of 89.6% and consistent accuracy during real-time field case studies of 89%. The test outcomes validate that the proposed 3D-LiDAR-based CM framework is suitable for outdoor mobile robots, assuring the robot’s health and operational safety

    Parvovirus b19 infection in Hong Kong

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    Human parvovirus B19 is a small DNA virus that can cause a number of diseases, notably erythema infectiosum in children, and aplastic crisis in patients with chronic haemolytic disorders. With the availability of serological tests for parvovirus infection, much is known about the prevalence of this virus in the Western population. However, there have not been any data on the epidemiological pattern of parvovirus B19 infection in Hong Kong and its surrounding region. In this report we have studied the sero-prevalence of parvovirus B19 in Hong Kong in 1983 and 1993, and were able to show a low incidence of parvovirus infection in the intervening 10 years, leading to a shift in the prevalence rate of parvovirus infection in the general population. From 1991 to 1996, of 276 patients presenting with illness which might have been caused by B19, only 2.5% were positive for IgM and 19.6% for IgG anti-B19. The prevalence of IgG anti-B19 among patients with thalassaemia major requiring frequent blood transfusion in 1995 was similar to those in the same age group in the general population, substantiating the observation that B19 infection was not common in Hong Kong in recent years.link_to_subscribed_fulltex

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    Not AvailableA 28-day experiment was carried out to evaluate different C/N ratios (10:1, 15:1 and 20:1) on growth performance, water quality, survival rate and gene expression related to metabolic pathways of Penaeus vannamei in a biofloc system. To maintain the C/N ratio of the feed input, different carbon sources (molasses, rice bran and wheat flour) in combination were applied after fermentation for a day with biofloc consortium. Results showed that shrimps reared in C/N 10 (630 mg) and C/N 15 (646 mg) biofloc systems showed significantly (p = .0229 and .0263) higher growth when compared to C/N 20 (528 mg) and control (374 mg). With regard to the production of extracellular enzymes, protease, lipase and xylanase were found predominantly in colonized bacteria isolated from biofloc treatments, whereas amylase was commonly found in all the treatments. The proPO activity was considerably increased in C/N 10 and 15 treatments than C/N 20 and control. The ranges of relative expression levels of potential genes entailed in metabolite pathway like hexokinase (HK- 6.57–8.17), pyruvate kinase (PK-5.79–7.96), crustacean hyperglycaemic hormone (CHH- 6.99–7.66) and fatty acid synthase (FAS- 6.57–8.86), triacylglycerol lipase (TGL-6.99–8.66) and immune gene (Toll receptor- 4.57–4.86) fold up-regulated expression were observed in the biofloc treatments than that of the control. The overall findings indicate that the rearing of P. vannamei shrimp in C/N 10 and 15 biofloc systems could improve the growth performance, water quality and development of immune and metabolic responses.Not Availabl

    Inhibition of calculi forming oxalate by dietary Basella rubra organs: Litholytic activity

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    The inhibition of calculi forming oxalate by dietary Basella rubra plant organs leaf and stem pod has been investigated. The weight reduction assay was studied. Also a concoction of the plant organs was tested. Leaf extract was found with considerable activity whereas the concoction seems to be not much active as the stem pod extract. Soluble oxalate of the plant organs are partially removed prior to extraction of active constituents. The active component/s seem to be a non-protein and non-tannin molecule/s that may act through inhibition of calcium accumulation there by proving the positive activity against the calculi or kidney stone. Regular consumption of leaf and stem pod extracts of our plant would be helpful in calculi prophylaxis
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