574 research outputs found
A case of a traumatic chyle leak following an acute thoracic spine injury: successful resolution with strict dietary manipulation
<p>Abstract</p> <p>Background</p> <p>Chylothorax is a rare form of pleural effusion that can be associated with both traumatic and non-traumatic causes. Thoracic duct ligation is often the treatment of choice in postsurgical patients; however the optimal treatment of this disease process after traumatic injury remains unclear <abbrgrp><abbr bid="B1">1</abbr></abbrgrp>. We present a rare case of a thoracic duct injury secondary to a blunt thoracic spine fracture and subluxation which was successfully treated non-operatively.</p> <p>Case Presentation</p> <p>A 51 year old male presented as a tier one trauma code due to an automobile versus bicycle collision. His examination and radiographic work-up revealed fractures and a subluxation at the third and fourth thoracic spine levels resulting in paraplegia. He also sustained bilateral hemothoraces secondary to multiple rib fractures. Drainage of the left hemothorax led to the diagnosis of a traumatic chylothorax. The thoracic spine fractures were addressed with surgical stabilization and the chylothorax was successfully treated with drainage and dietary manipulation.</p> <p>Conclusions</p> <p>This unusual and complex blunt thoracic duct injury required a multidisciplinary approach. Although the spine injury required surgical fixation, successful resolution of the chyle leak was achieved without surgical intervention.</p
Bacteriophage lambda cro mutations: effects on activity and intracellular degradation.
Topological effects in ring polymers: A computer simulation study
Unconcatenated, unknotted polymer rings in the melt are subject to strong
interactions with neighboring chains due to the presence of topological
constraints. We study this by computer simulation using the bond-fluctuation
algorithm for chains with up to N=512 statistical segments at a volume fraction
\Phi=0.5 and show that rings in the melt are more compact than gaussian chains.
A careful finite size analysis of the average ring size R \propto N^{\nu}
yields an exponent \nu \approx 0.39 \pm 0.03 in agreement with a Flory-like
argument for the topologica interactions. We show (using the same algorithm)
that the dynamics of molten rings is similar to that of linear chains of the
same mass, confirming recent experimental findings. The diffusion constant
varies effectively as D_{N} \propto N^{-1.22(3) and is slightly higher than
that of corresponding linear chains. For the ring sizes considered (up to 256
statistical segments) we find only one characteristic time scale \tau_{ee}
\propto N^{2.0(2); this is shown by the collapse of several mean-square
displacements and correlation functions onto corresponding master curves.
Because of the shrunken state of the chain, this scaling is not compatible with
simple Rouse motion. It applies for all sizes of ring studied and no sign of a
crossover to any entangled regime is found.Comment: 20 Pages,11 eps figures, Late
Altered expression of cyclin A 1 in muscle of patients with facioscapulohumeral muscle dystrophy (FSHD-1)
Objectives
Cyclin A1 regulates cell cycle activity and proliferation in somatic and germ-line cells. Its expression increases in G1/S phase and reaches a maximum in G2 and M phases. Altered cyclin A1 expression might contribute to clinical symptoms in facioscapulohumeral muscular dystrophy (FSHD).
Methods
Muscle biopsies were taken from the Vastus lateralis muscle for cDNA microarray, RT-PCR, immunohistochemistry and Western blot analyses to assess RNA and protein expression of cyclin A1 in human muscle cell lines and muscle tissue. Muscle fibers diameter was calculated on cryosections to test for hypertrophy.
Results
cDNA microarray data showed specifically elevated cyclin A1 levels in FSHD vs. other muscular disorders such as caveolinopathy, dysferlinopathy, four and a half LIM domains protein 1 deficiency and healthy controls. Data could be confirmed with RT-PCR and Western blot analysis showing up-regulated cyclin A1 levels also at protein level. We found also clear signs of hypertrophy within the Vastus lateralis muscle in FSHD-1 patients.
Conclusions
In most somatic human cell lines, cyclin A1 levels are low. Overexpression of cyclin A1 in FSHD indicates cell cycle dysregulation in FSHD and might contribute to clinical symptoms of this disease
The three-dimensional structure of the aspartate receptor from Escherichia coli
The crystal structure of the periplasmic domain of the aspartate receptor from Escherichia coli has been solved and refined to an R-factor of 0.203 at 2.3 Å, resolution. The dimeric protein is largely helical, with four helices from each monomer forming a four-helix bundle. The dimer interface is constructed from four helices, two from each subunit, also packed together in a four-helix bundle arrangement. A sulfate ion occupies the aspartate-binding site. All hydrogen bonds made to aspartate are substituted by direct or water-mediated hydrogen bonds to the sulfate. Comparison of the Escherichia coli aspartate-receptor structure with that of Salmonella typhimurium [Milburn, Prive, Milligan, Scott, Yeh, Jancarik, Koshland & Kim (1991). Science, 254, 1342-1347; Scott, Milligan, Milburn, Prive, Yeh, Koshland & Kim (1993). J. Mol. Biol. 232, 555-573] reveals strong conservation in the structure of the monomer, but more divergence in the orientation of the subunits with respect to one another. Mutations that render the Escherichia coli receptor incapable of responding to maltose are either located in spatially conserved sites or in regions of the structures that have high temperature factors and are therefore likely to be quite flexible. The inability of the receptor from Salmonella typhimurium to respond to maltose may, therefore, be because of differences in amino acids located on the binding surface rather than structural differences
Distribution of roots of random real generalized polynomials
The average density of zeros for monic generalized polynomials,
, with real holomorphic and
real Gaussian coefficients is expressed in terms of correlation functions of
the values of the polynomial and its derivative. We obtain compact expressions
for both the regular component (generated by the complex roots) and the
singular one (real roots) of the average density of roots. The density of the
regular component goes to zero in the vicinity of the real axis like
. We present the low and high disorder asymptotic
behaviors. Then we particularize to the large limit of the average density
of complex roots of monic algebraic polynomials of the form with real independent, identically distributed
Gaussian coefficients having zero mean and dispersion . The average density tends to a simple, {\em universal}
function of and in the domain where nearly all the roots are located for
large .Comment: 17 pages, Revtex. To appear in J. Stat. Phys. Uuencoded gz-compresed
tarfile (.66MB) containing 8 Postscript figures is available by e-mail from
[email protected]
Which position in American Football is more likely to get you benched due to LEI? An Analysis of NFL Players injured in the years 2016-2020.
Title: Which position in American Football is more likely to get you benched due to LEI? An Analysis of NFL Players injured in the years 2016-2020.
Authors: Robert de la Torre, Abdullah Sahyouni, Kinan Sawar, Gautham Pavar, Cris J. Diaz Alvarenga, Shravan Morisetty, Justin Bennie, Bohdan Matsko, Niyaz Uddin, Olivia Pakula
Introduction:
In American Football, there are twenty-four different positional roles that a player may assume. Some positions are unique to offense, such as quarterback and wide receiver. Others are unique to defense, such as cornerback or linebacker. There are also unique positions such as kicker or punter. All of these positions have different roles on the field and thus, different kinesiological patterns. These differences may lead to a variation in LE injuries experienced by players in different positions. Due to the prevalence of LEI\u27s in the NFL, we wanted to look at the trends for different injuries to see if there is positional correlation.
Methods:
We examined the NFL’s weekly injury reports for seasons from 2016 to 2020, and recorded players with four different categories of lower extremity injuries (LEI): (Hamstring, Calves, Groin, or Quadricep). Both the positional data and the nature of the injury are presented on NFL.com. We are looking at the frequency of various injuries in relation to position, as well as injury timelines for the four different LE injury categories.
Results:
Data is available and pending analysis.
Discussion:
The data collected from this study may be beneficial for any athlete engaging in organized football. Individuals with a certain injury history can be made aware of the impact their specific roles on the field may have on their injury. This can be useful information for parents and coaching staff/trainers to be aware of. Injuries to key players can dramatically impede a football season\u27s success in both monetary and subjective terms
Chiral Cilia orientation in the left-right organizer
Chirality is a property of asymmetry between an object and its mirror image. Most biomolecules and many cell types are chiral. In the left-right organizer (LRO), cilia-driven flows transfer such chirality to the body scale. However, the existence of cellular chirality within tissues remains unknown. Here, we investigate this question in Kupffer’s vesicle (KV), the zebrafish LRO. Quantitative live imaging reveals that cilia populating the KV display asymmetric orientation between the right and left sides, resulting in a chiral structure, which is different from the chiral cilia rotation. This KV chirality establishment is dynamic and depends on planar cell polarity. While its impact on left-right (LR) symmetry breaking remains unclear, we show that this asymmetry does not depend on the LR signaling pathway or flow. This work identifies a different type of tissue asymmetry and sheds light on chirality genesis in developing tissues
On a Generalization of Zaslavsky's Theorem for Hyperplane Arrangements
We define arrangements of codimension-1 submanifolds in a smooth manifold
which generalize arrangements of hyperplanes. When these submanifolds are
removed the manifold breaks up into regions, each of which is homeomorphic to
an open disc. The aim of this paper is to derive formulas that count the number
of regions formed by such an arrangement. We achieve this aim by generalizing
Zaslavsky's theorem to this setting. We show that this number is determined by
the combinatorics of the intersections of these submanifolds.Comment: version 3: The title had a typo in v2 which is now fixed. Will appear
in Annals of Combinatorics. Version. 2: 19 pages, major revision in terms of
style and language, some results improved, contact information updated, final
versio
Language, Sexuality and Inclusive Pedagogy
This paper examines linguistic practices of inclusion and exclusion relating to sexual orientation and sexual identity as they surface in the context of language education and multilingual contexts. I argue that queer linguistics can provide a helpful theoretical framework for examining how normative and non‐normative constructions of sexual identity are enacted inscribed in language practices in classrooms, and how these language practices may effect particular discourses of sexuality. I examine extracts of interview data with young people analysed using APPRAISAL analysis. The analysis focuses on how language works as a form of social practice which can include and exclude sexual identities in classroom settings
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