Increase in environmental temperature affects exploratory behaviour, anxiety and social preference in Danio rerio

The aim of this work is to investigate the effect of a temperature increase on the behaviour of adult zebrafish (Danio rerio) maintained for 21 days at 34 °C (treatment) and 26 °C (control). The temperatures chosen are within the vital range of zebrafish and correspond to temperatures that this species encounters in the natural environment. Previous results showed that the same treatment affects the brain proteome and the behaviour of adult zebrafish by producing alterations in the proteins involved in neurotransmitter release and synaptic function and impairing fish exploratory behaviour. In this study, we have investigated the performance of treated and control zebrafish during environmental exploration by using four behavioural tests (novel tank diving, light and dark preference, social preference and mirror biting) that are paradigms for assessing the state of anxiety, boldness, social preference and aggressive behaviour, respectively. The results showed that heat treatment reduces anxiety and increases the boldness of zebrafish, which spent more time in potentially dangerous areas of the tank such as the top and the uncovered bright area and at a distance from the social group, thus decreasing protection for the zebrafish. These data suggest that the increase in ambient temperature may compromise zebrafish survival rate in the natural environment

Gapless color-flavor locked phase in quark and hybrid stars

We study the effects of the gapless color-flavor locked (gCFL) phase on the equation of state of strongly interacting matter in the range of baryonic chemical potential involved in a compact star. We analyze the possibility of a phase transition from hadronic matter to gCFL quark matter and we discuss, for different values of the strange quark mass and diquark coupling strength, the existence of a gCFL phase in quark or hybrid stars. The mass-radius relation and the structure of compact stars containing the gCFL phase are shown and the physical relevance of this superconducting phase inside a stellar object is also discussed.Comment: 7 pages, 11 figure

Is there Quark Matter in (Low-Mass) Pulsars?

The effect of the QCD phase transition is studied for the mass-radius relation of compact stars and for hot and dense matter at a given proton fraction used as input in core-collapse supernova simulations. The phase transitions to the 2SC and CFL color superconducting phases lead to stable hybrid star configurations with a pure quark matter core. In supernova explosions quark matter could be easily produced due to $\beta$-equilibrium, small proton fractions and nonvanishing temperatures. A low critical density for the phase transition to quark matter is compatible with present pulsar mass measurements.Comment: 4 pages, 3 figures, talk given at the QM2008 conference, Jaipur, India, February 4-10, 2008, JPG in pres

Trends in hydroidomedusan research from 1911 to 1997

The papers on hydroidomedusae published from 1911 to 1997 total 10,934. They have been assigned to the following categories: faunistics and systematics; sub-organismal biology; ecology; evolution; life cycles; paleontology. The general trend, comprising all papers, can be divided into four time intervals: the first (1911-1939) with an average of sixty papers/year and with a slight decrease due to First World War; the second one (1940-1947), with an average of 38 papers/year, marked by a dramatic decrease coinciding with Second World War; the period 1948-1991 shows a steady increase until the mid-Seventies, when a small decrease occurred, followed by an increasing trend reaching its apex in the late Eighties-early Ninenties with a record of 296 papers in 1991 and with an average of 175 papers/year; the period 1992 1997, with an average of 178 papers/year, is marked by a sharp decrease, reaching the values of the mid Sixties. The most important category in terms of number of papers is sub-organismal biology, followed by faunistics and systematics. Systematic studies dictated the trend in the first decades of the century, whereas sub-organismal ones are prevalent from the Sixties onwards. Faunistic and systematic-taxonomic papers have a steady trend of production, with just a slight decrease over these last years. The formerly leading countries in systematics (UK, USA, France) are now almost inactive in this discipline, whereas countries with little or no tradition in this field (such as Spain) are taking the leadership

A label-free microfluidic assay to quantitatively study antibiotic diffusion through lipid membranes

PublishedJournal ArticleResearch Support, Non-U.S. Gov'tWith the rise in antibiotic resistance amongst pathogenic bacteria, the study of antibiotic activity and transport across cell membranes is gaining widespread importance. We present a novel, label-free microfluidic assay that quantifies the permeability coefficient of a broad spectrum fluoroquinolone antibiotic, norfloxacin, across lipid membranes using the UV autofluorescence of the drug. We use giant lipid vesicles as highly controlled model systems to study the diffusion through lipid membranes. Our technique directly determines the permeability coefficient without requiring the measurement of the partition coefficient of the antibiotic.This work was supported by a European Research Council (ERC) grant (261101 PassMembrane) to UFK. JC acknowledges support from an Internal Graduate Studentship, Trinity College, Cambridge. CC is supported by the ERC. SP acknowledges the support of the Leverhulme Trust and the Newton Trust through an Early Career Fellowship. AJ is supported by the Mexican National Council of Science and Technology. We thank Thomas Muller for help with the lithography and Tuomas Knowles for the use of his lithography facilitie

Late Summer Phytoplankton Blooms in the Changing Polar Environment of the Kongsfjorden (Svalbard, Arctic)

Kongsfjorden (Spitsbergen, Svalbard) is an inlet treated as a model site for studies on the impact of climate change in the Arctic due to its hydrological features. In this research, seven-days monitoring was carried out to evaluate the effects of hydrological variability on phytoplankton biomass and diversity in the late summer period. Temperature, salinity, nutrients, total suspended matter, phytoplankton abundance and biomass were determined for each sample. The thermo-haline properties of the column water seemed to affect phytoplankton communities. Their abundances and biomass were correlated with the amount of the total suspended matter. Moreover, species composition and biomass dramatically changed throughout the study period. Cold-water and Atlantic species were replaced by temperatewarm water dinoflagellates, including harmful species. An increase in phytoplankton biomass as well as the presence of dinoflagellate aggregations, mainly composed of Prorocentrum cf. gracile, were detected. This kind of algal accumulation is a new phenomenon in the Arctic and was probably related to the mobilization of sediment-rich glacial meltwaters. These findings, even if preliminary, suggest the need to study how additional biomass pulses and the increase of harmful species may alter the food web structure and the biogeochemical cycles, leading to major ecosystem changes

Strange quark matter in explosive astrophysical systems

Explosive astrophysical systems, such as supernovae or compact star binary mergers, provide conditions where strange quark matter can appear. The high degree of isospin asymmetry and temperatures of several MeV in such systems may cause a transition to the quark phase already around saturation density. Observable signals from the appearance of quark matter can be predicted and studied in astrophysical simulations. As input in such simulations, an equation of state with an integrated quark matter phase transition for a large temperature, density and proton fraction range is required. Additionally, restrictions from heavy ion data and pulsar observation must be considered. In this work we present such an approach. We implement a quark matter phase transition in a hadronic equation of state widely used for astrophysical simulations and discuss its compatibility with heavy ion collisions and pulsar data. Furthermore, we review the recently studied implications of the QCD phase transition during the early post-bounce evolution of core-collapse supernovae and introduce the effects from strong interactions to increase the maximum mass of hybrid stars. In the MIT bag model, together with the strange quark mass and the bag constant, the strong coupling constant $\alpha_s$ provides a parameter to set the beginning and extension of the quark phase and with this the mass and radius of hybrid stars.Comment: 6 pages, 5 figures, talk given at the International Conference on Strangeness in Quark Matter (SQM2009), Buzios, Brasil, September 28 - October 2, 2009, to be published in Journal Phys.

Strangeness in Astrophysics and Cosmology

Some recent developments concerning the role of strange quark matter for astrophysical systems and the QCD phase transition in the early universe are addressed. Causality constraints of the soft nuclear equation of state as extracted from subthreshold kaon production in heavy-ion collisions are used to derive an upper mass limit for compact stars. The interplay between the viscosity of strange quark matter and the gravitational wave emission from rotation-powered pulsars are outlined. The flux of strange quark matter nuggets in cosmic rays is put in perspective with a detailed numerical investigation of the merger of two strange stars. Finally, we discuss a novel scenario for the QCD phase transition in the early universe, which allows for a small inflationary period due to a pronounced first order phase transition at large baryochemical potential.Comment: 8 pages, invited talk given at the International Conference on Strangeness in Quark Matter (SQM2009), Buzios, Brasil, September 28 - October 2, 200

Magnetic field generated by r-modes in accreting quark stars

We show that the r-mode instability can generate strong toroidal fields in the core of accreting millisecond quark stars by inducing differential rotation. We follow the spin frequency evolution on a long time scale taking into account the magnetic damping rate in the evolution equations of r-modes. The maximum spin frequency of the star is only marginally smaller than in the absence of the magnetic field. The late-time evolution of the stars which enter the r-mode instability region is instead rather different if the generated magnetic fields are taken into account: they leave the millisecond pulsar region and they become radio pulsars.Comment: 8 pages, 8 figure

Production of benzaldehyde, benzyl alcohol and benzoic acid by yeasts and Botrytis cinerea isolated from grape musts and wines

The capacity of 100 yeast strains - isolated from grape musts and wines from the Istituto Sperimentale per l'Enologia collection - to produce benzaldehyde, benzyl alcohol and benzoic acid was verified by inoculation into a synthetic nutrient medium (MNS). Schizosaccharomyces and Zygosaccharomyces were strongest in producing benzaldehyde (maximal amount found 1200 µg/l) and benzyl alcohol (maximally 523 µg/l). Zygosaccharomyces was also most effective in the production of benzoic acid (maximally 536 µg/l), followed by Saccharomyces, Cryptococcus, Kloeckera and Torulaspora. The hypothesis was verified that yeasts can be an exogenous source of the benzyl alcohol oxidizing enzyme in grape musts and wines. Wine yeast strains of Saccharomyces spp., Zygosaccharomyces spp. and Schizosaccharomyces spp. fermenting MNS containing 150 g/l glucose, with benzyl alcohol added, transformed this into benzoic acid only when glucose was disappearing, but not into benzaldehyde. No difference was observed between aerobic and anaerobic fermentation conditions. The uptake of benzyl alcohol was rapid in fermentation essays in presence of only 10 g/l glucose and in assimilation essais performed in yeast nitrogen base broth with assimilable carbon compounds added. A catabolic repression by glucose appears likely. Botrytis cinerea was able to transform benzyl alcohol into benzaldehyde and benzoic acid on Czapek-Dox broth with 30 g/l sucrose added. Benzyl alcohol was transformed by wine yeasts into benzoic acid when the concentration of glucose in the mineral medium was less than 10 g/l, but no production of benzaldehyde was observed. A catabolic repression of this transformation by glucose is likely. Botrytis cinerea was able to produce benzaldehyde in a mineral medium with benzyl alcohol and sucrose added