Aero-acoustic tests of duct-burning turbofan exhaust nozzles

The acoustic and aerodynamic characteristics of several exhaust systems suitable for duct burning turbofan engines are evaluated. Scale models representing unsuppressed coannular exhaust systems are examined statically under varying exhaust conditions. Ejectors with both hardwall and acoustically treated inserts are investigated

Aerodynamic and acoustic tests of duct-burning turbofan exhaust nozzles

The static aerodynamic and acoustic characteristics of duct-burning turbofan (DBTF) exhaust nozzles are established. Scale models, having a total area equivalent to a 0.127 m diameter convergent nozzle, simulating unsuppressed coannular nozzles and mechanically suppressed nozzles with and without ejectors (hardwall and acoustically treated) were tested in a quiescent environment. The ratio of fan to primary area was varied from 0.75 to 1.2. Far field acoustic data, perceived noise levels, and thrust measurements were obtained for 417 test conditions. Pressure ratios were varied from 1.3 to 4.1 in the fan stream and from 1.53 to 2.5 in the primary stream. Total temperature varied from 395 to 1090 K in both streams. Jet noise reductions relative to synthesized prediction from 8 PNdB (with the unsuppressed coannular nozzle) to 15 PNdB (with a mechanically suppressed configuration) were observed at conditions typical of engines being considered under the Advanced Supersonic Technology program. The inherent suppression characteristic of the unsuppressed coannular nozzle is related to the rapid mixing in the jet wake caused by the velocity profiles associated with the DBTF. Since this can be achieved without a mechanical suppressor, significant reductions in aircraft weight or noise footprint can be realized

Flight effects on the aero/acoustic characteristics of inverted profile coannular nozzles

The effect of simulated flight speed on the acoustic and aerodynamic characteristics of coannular nozzles is examined. The noise and aerodynamic performance of the coannular nozzle exhaust systems over a large range of operating flight conditions is presented. The jet noise levels of the coannular nozzles are discussed. The impact of fan to primary nozzle area ratio and the presence of an ejector on flight effects are investigated. The impact of flight speed on the individual components of the coannular jet noise was ascertained

Flight effects on the aerodynamic and acoustic characteristics of inverted profile coannular nozzles

The effect of forward flight on the jet noise of coannular exhaust nozzles, suitable for Variable Stream Control Engines (VSCE), was investigated in a series of wind tunnel tests. The primary stream properties were maintained constant at 300 mps and 394 K. A total of 230 acoustic data points was obtained. Force measurement tests using an unheated air supply covered the same range of tunnel speeds and nozzle pressure ratios on each of the nozzle configurations. A total of 80 points was taken. The coannular nozzle OASPL and PNL noise reductions observed statically relative to synthesized values were basically retained under simulated flight conditions. The effect of fan to primary stream area ratio on flight effects was minor. At take-off speed, the peak jet noise for a VSCE was estimated to be over 6 PNdB lower than the static noise level. High static thrust coefficients were obtained for the basic coannular nozzles, with a decay of 0.75 percent at take-off speeds

Early intervention for stuttering: similarities and differences between two programs

In this paper, two proponents of different approaches to early stuttering discuss how they would manage a hypothetical case of stuttering in a preschool-age girl. Two evidence-based approaches are discussed: Palin Parent-Child Interaction Therapy and the Lidcombe Program. This paper arose from an oral discussion session held at the International Fluency Association Conference in Tours, France in 2012

Preserving Family: Themes from a Qualitative Study of Kin Caregivers

This article presents themes from a qualitative study of 58 African American female kinship caregivers in San Francisco. Core concepts that emerged describe various paths along which children move into kin homes, and caregivers\u27 mixed emotional reactions to becoming surrogate parents. Women also discussed multiple family roles they assumed after taking in children. Responses highlight three primary reasons for becoming caregivers that center on providing for and protecting these children—particularly from the perceived threat of the public foster care system—and ultimately preserving the family unit. Paradoxically, caregivers\u27 reasons mirror the stated goals of the public foster care system, which they view as a threat to family stability. We discuss the problems of implementing practice and policy recommendations for permanency and family preservation and how to bridge the gap between the deeply held negative beliefs of African American caregivers towards the public system and begin to build trust

A leucine-rich repeat peptide derived from the Drosophila Toll receptor forms extended filaments with a β-sheet structure

AbstractLeucine-rich repeats (LRRs) are 22–28 amino acid-long sequence motifs found in a family of cytoplasmic, membrane and extracellular proteins. There is evidence that LRRs function in signal transduction, cellular adhesion and protein-protein interactions. Here we report unusual properties of a synthetic LRR peptide derived from the sequence of the Drosophila membrane receptor Toll. In neutral solution the peptide forms a gel revealed by electron microscopy to consist of extended filaments approximately 8 nm in thickness. As the gel forms, the circular dichroism spectrum of the peptide solution changes from one characteristic of random coil to one associated with β-sheet structures. Molecular modelling suggests that the peptides form an amphipathic structure with a predominantly apolar and charged surface. Based on these results, models for the gross structure of the peptide filaments and a possible molecular mechanism for cellular adhesion are proposed

A leucine-rich repeat peptide derived from the Drosophila Toll receptor forms extended filaments with a β-sheet structure

AbstractLeucine-rich repeats (LRRs) are 22–28 amino acid-long sequence motifs found in a family of cytoplasmic, membrane and extracellular proteins. There is evidence that LRRs function in signal transduction, cellular adhesion and protein-protein interactions. Here we report unusual properties of a synthetic LRR peptide derived from the sequence of the Drosophila membrane receptor Toll. In neutral solution the peptide forms a gel revealed by electron microscopy to consist of extended filaments approximately 8 nm in thickness. As the gel forms, the circular dichroism spectrum of the peptide solution changes from one characteristic of random coil to one associated with β-sheet structures. Molecular modelling suggests that the peptides form an amphipathic structure with a predominantly apolar and charged surface. Based on these results, models for the gross structure of the peptide filaments and a possible molecular mechanism for cellular adhesion are proposed

The recognition of collagen and triple-helical toolkit peptides by MMP-13: sequence specificity for binding and cleavage.

Remodeling of collagen by matrix metalloproteinases (MMPs) is crucial to tissue homeostasis and repair. MMP-13 is a collagenase with a substrate preference for collagen II over collagens I and III. It recognizes a specific, well-known site in the tropocollagen molecule where its binding locally perturbs the triple helix, allowing the catalytic domain of the active enzyme to cleave the collagen α chains sequentially, at Gly(775)-Leu(776) in collagen II. However, the specific residues upon which collagen recognition depends within and surrounding this locus have not been systematically mapped. Using our triple-helical peptide Collagen Toolkit libraries in solid-phase binding assays, we found that MMP-13 shows little affinity for Collagen Toolkit III, but binds selectively to two triple-helical peptides of Toolkit II. We have identified the residues required for the adhesion of both proMMP-13 and MMP-13 to one of these, Toolkit peptide II-44, which contains the canonical collagenase cleavage site. MMP-13 was unable to bind to a linear peptide of the same sequence as II-44. We also discovered a second binding site near the N terminus of collagen II (starting at helix residue 127) in Toolkit peptide II-8. The pattern of binding of the free hemopexin domain of MMP-13 was similar to that of the full-length enzyme, but the free catalytic subunit bound none of our peptides. The susceptibility of Toolkit peptides to proteolysis in solution was independent of the very specific recognition of immobilized peptides by MMP-13; the enzyme proved able to cleave a range of dissolved collagen peptides.This work was supported by a British Heart Foundation programme grant, RG/009/003/27122, and peptide synthesis, by grants from Medical Research Council and Wellcome Trust.This is the author accepted manuscript. The final version can be found on the publisher's website at: http://www.jbc.org/content/early/2014/07/09/jbc.M114.58344

Ecological and Genomic Attributes of Novel Bacterial Taxa That Thrive in Subsurface Soil Horizons.

While most bacterial and archaeal taxa living in surface soils remain undescribed, this problem is exacerbated in deeper soils, owing to the unique oligotrophic conditions found in the subsurface. Additionally, previous studies of soil microbiomes have focused almost exclusively on surface soils, even though the microbes living in deeper soils also play critical roles in a wide range of biogeochemical processes. We examined soils collected from 20 distinct profiles across the United States to characterize the bacterial and archaeal communities that live in subsurface soils and to determine whether there are consistent changes in soil microbial communities with depth across a wide range of soil and environmental conditions. We found that bacterial and archaeal diversity generally decreased with depth, as did the degree of similarity of microbial communities to those found in surface horizons. We observed five phyla that consistently increased in relative abundance with depth across our soil profiles: Chloroflexi, Nitrospirae, Euryarchaeota, and candidate phyla GAL15 and Dormibacteraeota (formerly AD3). Leveraging the unusually high abundance of Dormibacteraeota at depth, we assembled genomes representative of this candidate phylum and identified traits that are likely to be beneficial in low-nutrient environments, including the synthesis and storage of carbohydrates, the potential to use carbon monoxide (CO) as a supplemental energy source, and the ability to form spores. Together these attributes likely allow members of the candidate phylum Dormibacteraeota to flourish in deeper soils and provide insight into the survival and growth strategies employed by the microbes that thrive in oligotrophic soil environments.IMPORTANCE Soil profiles are rarely homogeneous. Resource availability and microbial abundances typically decrease with soil depth, but microbes found in deeper horizons are still important components of terrestrial ecosystems. By studying 20 soil profiles across the United States, we documented consistent changes in soil bacterial and archaeal communities with depth. Deeper soils harbored communities distinct from those of the more commonly studied surface horizons. Most notably, we found that the candidate phylum Dormibacteraeota (formerly AD3) was often dominant in subsurface soils, and we used genomes from uncultivated members of this group to identify why these taxa are able to thrive in such resource-limited environments. Simply digging deeper into soil can reveal a surprising number of novel microbes with unique adaptations to oligotrophic subsurface conditions