14 research outputs found
Transcriptional Activity of the Islet β Cell Factor Pdx1 is Augmented by Lysine Methylation Catalyzed by the Methyltransferase Set7/9
The transcription factor Pdx1 is crucial to islet β cell function and regulates target genes in part through interaction with coregulatory factors. Set7/9 is a Lys methyltransferase that interacts with Pdx1. Here we tested the hypothesis that Lys methylation of Pdx1 by Set7/9 augments Pdx1 transcriptional activity. Using mass spectrometry and mutational analysis of purified proteins, we found that Set7/9 methylates the N-terminal residues Lys-123 and Lys-131 of Pdx1. Methylation of these residues occurred only in the context of intact, full-length Pdx1, suggesting a specific requirement of secondary and/or tertiary structural elements for catalysis by Set7/9. Immunoprecipitation assays and mass spectrometric analysis using β cells verified Lys methylation of endogenous Pdx1. Cell-based luciferase reporter assays using wild-type and mutant transgenes revealed a requirement of Pdx1 residue Lys-131, but not Lys-123, for transcriptional augmentation by Set7/9. Lys-131 was not required for high-affinity interactions with DNA in vitro, suggesting that its methylation likely enhances post-DNA binding events. To define the role of Set7/9 in β cell function, we generated mutant mice in which the gene encoding Set7/9 was conditionally deleted in β cells (SetΔβ). SetΔβ mice exhibited glucose intolerance similar to Pdx1-deficient mice, and their isolated islets showed impaired glucose-stimulated insulin secretion with reductions in expression of Pdx1 target genes. Our results suggest a previously unappreciated role for Set7/9-mediated methylation in the maintenance of Pdx1 activity and β cell function
A Comparison Of Weak Supervision Methods For Knowledge Base Construction
We present a comparison of weak and distant supervision methods for producing proxy examples for supervised relation extraction. We find that knowledge-based weak supervision tends to outperform popular distance supervision techniques, providing a higher yield of positive examples and more accurate models
Low Molecular Weight Amidoximes that Act as Potent Inhibitors of Lysine-Specific Demethylase 1
The recently discovered enzyme lysine-specific demethylase
1 (LSD1)
plays an important role in the epigenetic control of gene expression,
and aberrant gene silencing secondary to LSD1 dysregulation is thought
to contribute to the development of cancer. We reported that (bis)guanidines,
(bis)biguanides, and their urea- and thiourea isosteres are potent
inhibitors of LSD1 and induce the re-expression of aberrantly silenced
tumor suppressor genes in tumor cells in vitro. We now report a series
of small molecule amidoximes that are moderate inhibitors of recombinant
LSD1 but that produce dramatic changes in methylation at the histone
3 lysine 4 (H3K4) chromatin mark, a specific target of LSD1, in Calu-6
lung carcinoma cells. In addition, these analogues increase cellular
levels of secreted frizzle-related protein (SFRP) 2, H-cadherin (HCAD),
and the transcription factor GATA4. These compounds represent leads
for an important new series of drug-like epigenetic modulators with
the potential for use as antitumor agents