Development of a qualitative PCR method for the Alexandrium (Dinophyceae) detection in contaminated mussels (Mytilus galloprovincialis)

Paralytic shellfish poisoning (PSP) is a syndrome caused by the consumption of shellfish contaminated with neurotoxins produced by organisms of themarine dinoflagellate genus Alexandrium. A. minutum is the mostwidespread species responsible for PSP in theWestern Mediterranean basin. The standard monitoring of shellfish farms for the presence of harmful algae and related toxins usually requires the microscopic examination of phytoplankton populations, bioassays and toxin determination by HPLC. These procedures are time-consuming and require remarkable experience, thus limiting the number of specimens that can be analyzed by a single laboratory unit.Molecular biology techniques may be helpful in the detection of target microorganisms in field samples. In this study, we developed a qualitative PCR assay for the rapid detection of all potentially toxic species belonging to the Alexandrium genus and specifically A. minutum, in contaminated mussels. Alexandrium genus-specific primers were designed to target the 5.8S rDNA region, while an A. minutum species-specific primer was designed to bind in the ITS1 region. The assay was validated using several fixed seawater samples fromthe Mediterranean basin, which were analyzed using PCR along with standard microscopy procedures. The assay provided a rapid method for monitoring the presence of Alexandrium spp. in mussel tissues, as well as in seawater samples. The results showed that PCR is a valid, rapid alternative procedure for the detection of target phytoplankton species either in seawater or directly in mussels, where microalgae can accumulat

Fabrication and Results of the First MgB2 Round Coil Superferric Magnet at LASA

The LASA Laboratory (INFN, Milan) is working in the High Luminosity LHC program to develop, in collaboration with CERN, six different types of High Order corrector magnets. In this framework, in parallel with a conventional design of superferric magnets with LTS conductor, the LASA is focusing on the research of new superconducting materials which may have applications in particle accelerator magnets. To this purpose, LASA is developing a new type of superferric magnet suitable to arbitrary multipole order, called Round Coil Superferric Magnets (RCSM). The iron yoke shaped with an arbitrary number of poles is able to create the desired harmonic component using only one single round coil with a large bending radius suitable for very strain-sensitive superconductors. The electromagnetic design of a sextupole configuration of the magnet and the production of the first superconducting MgB2 round coil prototype have been already presented. In this paper, we expose the optimization of the iron yoke and polar expansions assembly of the first magnet semi-module prototype. The results of the whole powering test are described in detail and the analysis of the magnetic performances are compared with those of classical superferric correctors

Giant and tunable anisotropy of nanoscale friction in graphene

CNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOFAPERJ - FUNDAÇÃO CARLOS CHAGAS FILHO DE AMPARO À PESQUISA DO ESTADO DO RIO DE JANEIROFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOFAPEMIG - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE MINAS GERAISThe nanoscale friction between an atomic force microscopy tip and graphene is investigated using friction force microscopy (FFM). During the tip movement, friction forces are observed to increase and then saturate in a highly anisotropic manner. As a result, the friction forces in graphene are highly dependent on the scanning direction: under some conditions, the energy dissipated along the armchair direction can be 80% higher than along the zigzag direction. In comparison, for highly-oriented pyrolitic graphite (HOPG), the friction anisotropy between armchair and zigzag directions is only 15%. This giant friction anisotropy in graphene results from anisotropies in the amplitudes of flexural deformations of the graphene sheet driven by the tip movement, not present in HOPG. The effect can be seen as a novel manifestation of the classical phenomenon of Euler buckling at the nanoscale, which provides the non-linear ingredients that amplify friction anisotropy. Simulations based on a novel version of the 2D Tomlinson model (modified to include the effects of flexural deformations), as well as fully atomistic molecular dynamics simulations and first-principles density-functional theory (DFT) calculations, are able to reproduce and explain the experimental observations.The nanoscale friction between an atomic force microscopy tip and graphene is investigated using friction force microscopy (FFM). During the tip movement, friction forces are observed to increase and then saturate in a highly anisotropic manner. As a result, the friction forces in graphene are highly dependent on the scanning direction: under some conditions, the energy dissipated along the armchair direction can be 80% higher than along the zigzag direction. In comparison, for highly-oriented pyrolitic graphite (HOPG), the friction anisotropy between armchair and zigzag directions is only 15%. This giant friction anisotropy in graphene results from anisotropies in the amplitudes of flexural deformations of the graphene sheet driven by the tip movement, not present in HOPG. The effect can be seen as a novel manifestation of the classical phenomenon of Euler buckling at the nanoscale, which provides the non-linear ingredients that amplify friction anisotropy. Simulations based on a novel version of the 2D Tomlinson model (modified to include the effects of flexural deformations), as well as fully atomistic molecular dynamics simulations and first-principles density-functional theory (DFT) calculations, are able to reproduce and explain the experimental observations.619CNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOFAPERJ - FUNDAÇÃO CARLOS CHAGAS FILHO DE AMPARO À PESQUISA DO ESTADO DO RIO DE JANEIROFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOFAPEMIG - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE MINAS GERAISCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOFAPERJ - FUNDAÇÃO CARLOS CHAGAS FILHO DE AMPARO À PESQUISA DO ESTADO DO RIO DE JANEIROFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOFAPEMIG - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE MINAS GERAISSem informaçãoSem informação2013/08293-7, 2014/15521-9Sem informaçãoAll authors aknowledge the financial support from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ). R.P. acknowledges Fundação de Amparo a Pesquisa do Estado de São Paulo (Fapesp) for financial support through Grant #2014/15521-9. D.S.G. thanks the Center for Computational Engineering and Sciences at Unicamp for financial support through the FAPESP/CEPID Grant # 2013/08293-7. Computer simulations carried out during this research were supported by resources supplied by the Center for Scientific Computing (NCC/GridUNESP) of the São Paulo State University (UNESP). L.G.C. acknowledges FAPEMIG and the grant PRONAMETRO (52600.056330/2012). B.F acknowledges FAPEMIG and the grant PRONAMETRO (52600.030929/2014)

DIVERSIDAD DE LOS NEMATODOS DE TETRA COLA ROJA ASTYANAX AFF. PARANAE (TELEOSTEI: CHARACIDAE) DE SITIOS CONTAMINADOS DE UN SISTEMA DE RÍO TROPICAL

Aquatic systems are affected by a great variety of anthropogenic activities that alter the water quality through the introduction of organic and inorganic pollutants, consequently impacting all trophic levels of a biological community. The relationship between parasite species diversity and organic pollution in the high Paraná River basin, Paraná, Brazil was investigated during two seasons (Winter and Summer) of 2011. A total of 114 red-tail-lambari (Astyanax aff. paranae Eigenmann, 1914) were sampled from a non-impacted location (Perobas Reserve) and from impacted regions (agricultural and urban regions, upstream and downstream the city of Campo Mourão, respectively). The nematode Spinitectus yorkei Travassos, Artigas & Pereira, 1928 was found on the preserved area, while the nematode Procamallanus (Spirocamallanus) inopinatus Travassos, Artigas & Pereira, 1928 was found only on the severely polluted areas. The variability of the prevalence index suggests that the structure of the parasite community is affected at different ways by the same levels of pollutants on the water. The observed results assume that the alteration in parasite diversity can be related to increased organic pollution, and that the differences in the parasite community from different environments can be related to biotic stress.Los sistemas acuáticos se ven afectados por una gran variedad de actividades antropogénicas que alteran la calidad del agua a través de la introducción de contaminantes orgánicos e inorgánicos, por consiguiente, afectan a todos los niveles de la comunidad biológica. De esta forma, la relación entre la diversidad de especies de parásitos y la contaminación orgánica en la cuenca del alto Paraná, Paraná, Brasil, se investigó en dos temporadas (invierno y verano) de 2011. Un total de 114 tetra cola roja (Astyanax aff. paranae Eigenmann, 1914) se muestrearon en un sitio no impactado (Reserva Perobas) y en regiones impactadas (zonas agrícolas y urbanas, aguas arriba y aguas abajo de la ciudad de Campo Mourão, respectivamente). El nematodo Spinitectus yorkei Travassos, Artigas y Pereira, 1928 se encuentran en la zona protegida, mientras que el nematodo Procamallanus (Spirocamallanus) inopinatus Travassos, Artigas y Pereira, 1928 se encuentran sólo en las zonas gravemente contaminadas. La variabilidad del índice de prevalencia sugiere que la estructura de la comunidad de parásitos se ve afectada en diferentes formas por el mismo nivel de contaminantes en el agua. Los resultados observados asumen que la alteración en la diversidad parasitaria puede estar relacionada con el aumento de la contaminación orgánica, y que la respuesta de la comunidad de parásitos a las tensiones ambientales puede ser utilizada como bioindicadora

A Work Proposal for a Collaborative Study of Magnet Technology for a Future Muon Collider

In this paper we elaborate on the nature and challenges for the magnet systems of a muon collider as presently considered within the scope of the International Muon Collider Collaboration (IMCC). We outline the structure of the work proposed over the coming period of five years to study and demonstrate relevant magnet technology. The proposal, which is part of the overall work planned to establish feasibility of a muon collider, is in direct response to the recent recommendations received from the Laboratories Directors Group (LDG). The plan is to profit from joint activities, within the scope of the IMCC and beyond, implemented through direct and EU-funded contributions.Comment: contribution to Snowmass 202

A Muon Collider Facility for Physics Discovery

Muon colliders provide a unique route to deliver high energy collisions thatenable discovery searches and precision measurements to extend ourunderstanding of the fundamental laws of physics. The muon collider design aimsto deliver physics reach at the highest energies with costs, power consumptionand on a time scale that may prove favorable relative to other proposedfacilities. In this context, a new international collaboration has formed tofurther extend the design concepts and performance studies of such a machine.This effort is focused on delivering the elements of a $\sim$10 TeV center ofmass (CM) energy design to explore the physics energy frontier. The path tosuch a machine may pass through lower energy options. Currently a 3 TeV CMstage is considered. Other energy stages could also be explored, e.g. ans-channel Higgs Factory operating at 125 GeV CM. We describe the status of theR&D and design effort towards such a machine and lay out a plan to bring theseconcepts to maturity as a tool for the high energy physics community.<br

Risk of hospitalization for heart failure in patients with type 2 diabetes newly treated with DPP-4 inhibitors or other oral glucose-lowering medications: A retrospective registry study on 127,555 patients from the Nationwide OsMed Health-DB Database

Aims Oral glucose-lowering medications are associated with excess risk of heart failure (HF). Given the absence of comparative data among drug classes, we performed a retrospective study in 32 Health Services of 16 Italian regions accounting for a population of 18 million individuals, to assess the association between HF risk and use of sulphonylureas, DPP-4i, and glitazones. Methods and results We extracted data on patients with type 2 diabetes who initiated treatment with DPP-4i, thiazolidinediones, or sulphonylureas alone or in combination with metformin during an accrual time of 2 years. The endpoint was hospitalization for HF (HHF) occurring after the first 6 months of therapy, and the observation was extended for up to 4 years. A total of 127 555 patients were included, of whom 14.3% were on DPP-4i, 72.5% on sulphonylurea, 13.2% on thiazolidinediones, with average 70.7% being on metformin as combination therapy. Patients in the three groups differed significantly for baseline characteristics: age, sex, Charlson index, concurrent medications, and previous cardiovascular events. During an average 2.6-year follow-up, after adjusting for measured confounders, use of DPP-4i was associated with a reduced risk of HHF compared with sulphonylureas [hazard ratio (HR) 0.78; 95% confidence interval (CI) 0.62-0.97; P = 0.026]. After propensity matching, the analysis was restricted to 39 465 patients, and the use of DPP-4i was still associated with a lower risk of HHF (HR 0.70; 95% CI 0.52-0.94; P = 0.018). Conclusion In a very large observational study, the use of DPP-4i was associated with a reduced risk of HHF when compared with sulphonylureas

Assessment of atrial regional and global electromechanical function by tissue velocity echocardiography: a feasibility study on healthy individuals

BACKGROUND: The appropriate evaluation of atrial electrical function is only possible by means of invasive electrophysiology techniques, which are expensive and therefore not suitable for widespread use. Mechanical atrial function is mainly determined from atrial volumes and volume-derived indices that are load-dependent, time-consuming and difficult to reproduce because they are observer-dependent. AIMS: To assess the feasibility of tissue velocity echocardiography (TVE) to evaluate atrial electromechanical function in young, healthy volunteers. SUBJECTS AND METHODS: We studied 37 healthy individuals: 28 men and nine women with a mean age of 29 years (range 20–47). Standard two-dimensional (2-D) and Doppler echocardiograms with superimposed TVE images were performed. Standard echocardiographic images were digitized during three consecutive cardiac cycles in cine-loop format for off-line analysis. Several indices of regional atrial electrical and mechanical function were derived from both 2-D and TVE modalities. RESULTS: Some TVE-derived variables indirectly reflected the atrial electrical activation that follows the known activation process as revealed by invasive electrophysiology. Regionally, the atrium shows an upward movement of its walls at the region near the atrio-ventricular ring with a reduction of this movement towards the upper levels of the atrial walls. The atrial mechanical function as assessed by several TVE-derived indices was quite similar in all left atrium (LA) walls. However, all such indices were higher in the right (RA) than the LA. There were no correlations between the 2-D- and TVE-derived variables expressing atrial mechanical function. Values of measurement error and repeatability were good for atrial mechanical function, but only acceptable for atrial electrical function. CONCLUSION: TVE may provide a simple, easy to obtain, reproducible, repeatable and potentially clinically useful tool for quantifying atrial electromechanical function