Componentes de rendimento da mamoneira irrigada com diferentes lâminas de irrigação.

O trabalho foi conduzido na Fazenda Experimental Rafael Fernandes, município de Mossoró, RN, com o objetivo verificar o efeito de lâminas de irrigação sobre os componentes de rendimento da mamoneira, cultivar ?BRS ENERGIA?, irrigada por gotejamento. Para tanto, utilizou-se o delineamento experimental em blocos ao acaso, em esquema de parcelas subdivididas 5 x 2, com quatro repetições. Os tratamentos foram constituídos das lâminas de irrigação (0,6; 0,8; 1,0; 1,2; e 1,4 da ETc) nas parcelas principais e os espaçamentos nas sub-parcelas (1,5m x 0,30m x 0,30m e 1,5m x 0,30m x 0,40m). Foram tomados dados referentes ao número médio de bagas dos racemos principal e secundário, peso médio da baga (g) dos racemos principal e secundário e número de racemos secundários por planta. Número de bagas do racemo secundário e peso médio da baga do racemo principal apresentaram comportamento quadrático com as lâminas de irrigação aplicadas. O incremento na lâmina favoreceu o aumento do número de racemos secundários por planta

Delimiting the Origin of a B Chromosome by FISH Mapping, Chromosome Painting and DNA Sequence Analysis in Astyanax paranae (Teleostei, Characiformes)

Supernumerary (B) chromosomes have been shown to contain a wide variety of repetitive sequences. For this reason, fluorescent in situ hybridisation (FISH) is a useful tool for ascertaining the origin of these genomic elements, especially when combined with painting from microdissected B chromosomes. In order to investigate the origin of B chromosomes in the fish species Astyanax paranae, these two approaches were used along with PCR amplification of specific DNA sequences obtained from the B chromosomes and its comparison with those residing in the A chromosomes. Remarkably, chromosome painting with the one-arm metacentric B chromosome probe showed hybridization signals on entire B chromosome, while FISH mapping revealed the presence of H1 histone and 18S rDNA genes symmetrically placed in both arms of the B chromosome. These results support the hypothesis that the B chromosome of A. paranae is an isochromosome. Additionally, the chromosome pairs Nos. 2 or 23 are considered the possible B chromosome ancestors since both contain syntenic H1 and 18S rRNA sequences. The analysis of DNA sequence fragments of the histone and rRNA genes obtained from the microdissected B chromosomes showed high similarity with those obtained from 0B individuals, which supports the intraspecific origin of B chromosomes in A. paranae. Finally, the population hereby analysed showed a female-biased B chromosome presence suggesting that B chromosomes in this species could influence sex determinism.This research was funded by grants from the State of São Paulo Research Foundation (FAPESP) to DMZAS (2011/16825-3) and CO (2010/17009-2), grants from National Council for Research and Development (CNPq) to FF and by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

A922 Sequential measurement of 1 hour creatinine clearance (1-CRCL) in critically ill patients at risk of acute kidney injury (AKI)

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