βCaMKII regulates bidirectional long-term plasticity in cerebellar Purkinje cells by a CaMKII/PP2B switch mechanism

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The Phase Structure of the Weakly Coupled Lattice Schwinger Model

The weak coupling expansion is applied to the single flavour Schwinger model with Wilson fermions on a symmetric toroidal lattice of finite extent. We develop a new analytic method which permits the expression of the partition function as a product of pure gauge expectation values whose zeroes are the Lee-Yang zeroes of the model. Application of standard finite-size scaling techniques to these zeroes recovers previous numerical results for the small and moderate lattice sizes to which those studies were restricted. Our techniques, employable for arbitrarily large lattices, reveal the absence of accumulation of these zeroes on the real hopping parameter axis at constant weak gauge coupling. The consequence of this previously unobserved behaviour is the absence of a zero fermion mass phase transition in the Schwinger model with single flavour Wilson fermions at constant weak gauge coupling.Comment: 8 pages, 2 figures, insert to figure 2 include

Experimental assessment and retrofit of full-scale models of existing RC frames

PSD tests on two full-scale models of existing non-seismic resisting RC frame structures are described. The testing program covered several aspects, namely assessment of seismic performance of existing frames without and with infill panels, retrofitting of the bare frame using Selective Retrofitting techniques, strengthening of the infill panels using shotcrete and retrofitting of the frame using K-bracing with shear-link dissipators. The main results from the tests are summarized and discussed and the conclusions are drawn. The tests on the bare frame have shown how vulnerable are existing structures constructed in the 60’s and the beneficial effects of infill panels were confirmed from the tests on the infilled frame. Important improvements, in terms of seismic performance, were achieved by the retrofitting of the frames. However, it was also confirmed that strengthening of the existing infill panels in poorly detailed frames may lead to dangerous ‘local’ failures, such as the shear out of the external columns

The Structure of the Aoki Phase at Weak Coupling

A new method to determine the phase diagram of certain lattice fermionic field theories in the weakly coupled regime is presented. This method involves a new type of weak coupling expansion which is multiplicative rather than additive in nature and allows perturbative calculation of partition function zeroes. Application of the method to the single flavour Gross-Neveu model gives a phase diagram consistent with the parity symmetry breaking scenario of Aoki and provides new quantitative information on the width of the Aoki phase in the weakly coupled sector.Comment: 9 pages, 1 figure (minor changes) To be published in Phys. Lett.

PCR biases distort bacterial and archaeal community structure in pyrosequencing datasets

As 16S rRNA gene targeted massively parallel sequencing has become a common tool for microbial diversity investigations, numerous advances have been made to minimize the influence of sequencing and chimeric PCR artifacts through rigorous quality control measures. However, there has been little effort towards understanding the effect of multi-template PCR biases on microbial community structure. In this study, we used three bacterial and three archaeal mock communities consisting of, respectively, 33 bacterial and 24 archaeal 16S rRNA gene sequences combined in different proportions to compare the influences of (1) sequencing depth, (2) sequencing artifacts (sequencing errors and chimeric PCR artifacts), and (3) biases in multi-template PCR, towards the interpretation of community structure in pyrosequencing datasets. We also assessed the influence of each of these three variables on α- and β-diversity metrics that rely on the number of OTUs alone (richness) and those that include both membership and the relative abundance of detected OTUs (diversity). As part of this study, we redesigned bacterial and archaeal primer sets that target the V3–V5 region of the 16S rRNA gene, along with multiplexing barcodes, to permit simultaneous sequencing of PCR products from the two domains. We conclude that the benefits of deeper sequencing efforts extend beyond greater OTU detection and result in higher precision in β-diversity analyses by reducing the variability between replicate libraries, despite the presence of more sequencing artifacts. Additionally, spurious OTUs resulting from sequencing errors have a significant impact on richness or shared-richness based α- and β-diversity metrics, whereas metrics that utilize community structure (including both richness and relative abundance of OTUs) are minimally affected by spurious OTUs. However, the greatest obstacle towards accurately evaluating community structure are the errors in estimated mean relative abundance of each detected OTU due to biases associated with multi-template PCR reactions