Обеспечение точности обобщенной интервальной оценки качества изделий

Разработан метод интервальной оценки качества изделий, отличительной особенностью которого является получение весовых коэффициентов обобщенного показателя с использованием нечетких функций принадлежности гауссового типа. Их использование позволяет повысить точность и достоверность принятия решения при определении категории качества изделий различного целевого назначения в соответствии с вербально-числовой шкалой функции желательности Харрингтона.Розроблено метод інтервальної оцінки якості виробів, відмітною особливістю якого є отримання вагових коефіцієнтів узагальненого показника з використанням нечітких функцій приналежності гауссового типу. Їх використання дозволяє підвищити точність та вірогідність прийняття рішення під час визначення категорії якості виробів різного цільового призначення відповідно до вербально-числової шкали функції бажаності Харрінгтона.The method of interval estimation of the products’ quality, the distinctive feature of which is a receipt of gravimetric coefficients of the generalized index with the use of unclear membership functions of gauss type, is developed. Their usage allows to promote exactness and authenticity of decision-making at determination category of products’ quality of the different target destination in accordance with the verbally-numerical scale of desirability function of Harrington

Small cetacean bycatch as estimated from stranding schemes: The common dolphin case in the northeast Atlantic

Death in fishing gear of non-target species (called ‘bycatch’) is a major concern for marine wildlife, and mostly worrying for long-lived species like cetaceans, considering their demographic characteristics (slow population growth rates and low fecundity). In European waters, cetaceans are highly impacted by this phenomenon. Under the Common Fishery Policy, the EC 812/2004 regulation constitutes a legal frame for bycatch monitoring on 5–10% of fishing vessels >15 m. The aim of this work was to compare parameters and bycatch estimates of common dolphins (Delphinus delphis) provided by observer programmes in France and UK national reports and those inferred from stranding data, through two approaches. Bycatch was estimated from stranding data, first by correcting effectives from drift conditions (using a drift prediction model) and then by estimating the probability of being buoyant. Observer programmes on fishing vessels allowed us to identify the specificity of the interaction between common dolphins and fishing gear, and provided low estimates of annual bycaught animals (around 550 animals year−1). However, observer programmes are hindered by logistical and administrative constraints, and the sampling scheme seems to be poorly designed for the detection of marine mammal bycatches. The analyses of strandings by considering drift conditions highlighted areas with high levels of interactions between common dolphins and fisheries. Since 1997, the highest densities of bycaught dolphins at sea were located in the southern part of the continental shelf and slope of the Bay of Biscay. Bycatch numbers inferred from strandings suggested very high levels, ranging from 3650 dolphins year−1 [2250–7000] to 4700 [3850–5750] dolphins year−1, depending on methodological choices. The main advantage of stranding data is its large spatial scale, cutting across administrative boundaries. Diverging estimates between observer programmes and stranding interpretation can set very different management consequences: observer programmes suggest a sustainable situation for common dolphins, whereas estimates based on strandings highlight a very worrying and unsustainable process

Hepatic DNA damage in harbour porpoises (Phocoena phocoena) stranded along the English and Welsh coastlines

One level at which persistent organic pollutants (POPs) and polycyclic aromatic hydrocarbons PAHs) can exert damage is by causing DNA strand-breaks or nucleotide base modifications, which, if unrepaired, can lead to embryonic mutations, abnormal development and cancer. In marine ecosystems, genotoxicity is expected to be particularly strong in long-lived apex predators due to pollutant bioaccumulation. We conducted 32 P-postlabeling analyses optimized for the detection and quantification of aromatic/hydrophobic DNA adducts in the livers of 40 sexually-mature North Atlantic harbour porpoises (Phocoena phocoena) stranded along the English and Welsh coastlines. We examined hepatic tissue to search for inflammatory and preneoplastic lesions and examine their association with adduct levels. Adducts were found in all porpoises (mean: 17.56 ± 11.95 per 108 nucleotides), and were higher than levels reported for marine vertebrates from polluted sites. The pollutants causing the induced DNA adducts could not be further characterized. Hepatic DNA damage did not correlate with levels of blubber POP concentrations (including total polychlorinated biphenyl [PCBs], dichlorodiphenyltrichloroethane [DDT] and dieldrin); PAH concentrations were not available for the present study. However, DNA damage predicted occurrence of inflammatory and preneoplastic lesions. Further, our data showed a reduction in hepatic DNA adduct levels with age in the 40 animals examined while POP concentrations, particularly PCBs, increased with age. Using a different dataset of 145 mature male harbour porpoises confirmed that higher contaminant levels (total PCBs, DDT and dieldrin) are found in older animals. The reduction in hepatic DNA adduct levels in older animals was in accordance with other studies which show that suppression of hepatic CYP1A enzyme activity at high PCB concentrations might impact on CYP1A-mediated DNA adduct formation of PAHs which are ubiquitous environmental pollutants and readily metabolized by CYP1A to species binding to DNA. In summary, our study shows that pollutant-induced DNA damage is prevalent in harbour porpoises from UK waters and may lead to detectable sub-lethal hepatic damage

Phylogenomic Resolution of the Cetacean Tree of Life Using Target Sequence Capture.

The evolution of cetaceans, from their early transition to an aquatic lifestyle to their subsequent diversification, has been the subject of numerous studies. However, while the higher-level relationships among cetacean families have been largely settled, several aspects of the systematics within these groups remain unresolved. Problematic clades include the oceanic dolphins (37 spp.), which have experienced a recent rapid radiation, and the beaked whales (22 spp.), which have not been investigated in detail using nuclear loci. The combined application of high-throughput sequencing with techniques that target specific genomic sequences provide a powerful means of rapidly generating large volumes of orthologous sequence data for use in phylogenomic studies. To elucidate the phylogenetic relationships within the Cetacea, we combined sequence capture with Illumina sequencing to generate data for ∼3200 protein-coding genes for 68 cetacean species and their close relatives including the pygmy hippopotamus. By combining data from >38,000 exons with existing sequences from 11 cetaceans and seven outgroup taxa, we produced the first comprehensive comparative genomic dataset for cetaceans, spanning 6,527,596 aligned base pairs and 89 taxa. Phylogenetic trees reconstructed with maximum likelihood and Bayesian inference of concatenated loci, as well as with coalescence analyses of individual gene trees, produced mostly concordant and well-supported trees. Our results completely resolve the relationships among beaked whales as well as the contentious relationships among oceanic dolphins, especially the problematic subfamily Delphininae. We carried out Bayesian estimation of species divergence times using MCMCTree, and compared our complete dataset to a subset of clocklike genes. Analyses using the complete dataset consistently showed less variance in divergence times than the reduced dataset. In addition, integration of new fossils (e.g., Mystacodon selenensis) indicate that the diversification of Crown Cetacea began before the Late Eocene and the divergence of Crown Delphinidae as early as the Middle Miocene

PCB pollution continues to impact populations of orcas and other dolphins in European waters

Organochlorine (OC) pesticides and the more persistent polychlorinated biphenyls (PCBs) have well-established dose-dependent toxicities to birds, fish and mammals in experimental studies, but the actual impact of OC pollutants on European marine top predators remains unknown. Here we show that several cetacean species have very high mean blubber PCB concentrations likely to cause population declines and suppress population recovery. In a large pan-European meta-analysis of stranded (n = 929) or biopsied (n = 152) cetaceans, three out of four species:- striped dolphins (SDs), bottlenose dolphins (BNDs) and killer whales (KWs) had mean PCB levels that markedly exceeded all known marine mammal PCB toxicity thresholds. Some locations (e.g. western Mediterranean Sea, south-west Iberian Peninsula) are global PCB "hotspots" for marine mammals. Blubber PCB concentrations initially declined following a mid-1980s EU ban, but have since stabilised in UK harbour porpoises and SDs in the western Mediterranean Sea. Some small or declining populations of BNDs and KWs in the NE Atlantic were associated with low recruitment, consistent with PCB-induced reproductive toxicity. Despite regulations and mitigation measures to reduce PCB pollution, their biomagnification in marine food webs continues to cause severe impacts among cetacean top predators in European seas

Methodology for in situ gas sampling, transport and laboratory analysis of gases from stranded cetaceans

Gas-bubble lesions were described in cetaceans stranded in spatio-temporal concordance with naval exercises using high-powered sonars. A behaviourally induced decompression sickness-like disease was proposed as a plausible causal mechanism, although these findings remain scientifically controversial. Investigations into the constituents of the gas bubbles in suspected gas embolism cases are highly desirable. We have found that vacuum tubes, insulin syringes and an aspirometer are reliable tools for in situ gas sampling, storage and transportation without appreciable loss of gas and without compromising the accuracy of the analysis. Gas analysis is conducted by gas chromatography in the laboratory. This methodology was successfully applied to a mass stranding of sperm whales, to a beaked whale stranded in spatial and temporal association with military exercises and to a cetacean chronic gas embolism case. Results from the freshest animals confirmed that bubbles were relatively free of gases associated with putrefaction and consisted predominantly of nitrogen