In-System Programmer for ICSP Interface of Pic Microcontrollers

Tato práce se zabývá návrhem programátoru pro programování mikrokontrolérů Microchip PIC 16F a 18F. Její součástí je návrh zapojení, deska plošných spojů a obslužná aplikace. Navržený programátor je osazen mikrokontrolérem PIC18F67J50, grafickým displejem a paměťovou kartou. Konstrukce umožňuje snadné přizpůsobení většině cílových součástek. V práci jsou popsány principy programování pomocí protokolu ICSP firmy Microchip se zaměřením na zmíněné rodiny procesorů. Součástí práce je funkční programátor.This thesis is dealing with a design of programming device with the expected use for Microchip PIC 16F and 18F microcontrollers. Individual chapters are dedicated to aspects of circuitry, printed circuit board design and user software tools. The proposed device is based on PIC18F67J50 microcontroller, graphic LCD diplay, and memory card interface. Design can be easily adjusted in order to support wide range of devices. Microchip's In-Circuit Serial Programming protocol is described with focus on previously mentioned family of devices. Working prototype of the programmer is attached to this thesis as a demonstration of a practical outcome.   

Traffic Shaping in High Speed Networks in DPDK

Předmětem této bakalářské práce je plánování síťového provozu ve vysokorychlostních sítích. V práci je popsán framework DPDK, který lze využít pro rychlé zpracovávání paketů. Jsou popsány obecné mechanismy plánování síťového provozu a plánování provozu v Linuxu pomocí nástroje tc. Dále je představen návrh a implementace plánovače síťového provozu v prostředí DPDK pro sítě o šířce pásma 10 Gbps. Pro plánovač je použit komplexní mechanismus hierarchického modelu zásobníku žetonů. Systém byl otestován pomocí generátoru síťového provozu Spirent.This bachelor thesis is focused on traffic shaping in high speed networks. It presents framework DPDK, which can be used for fast packet processing. General traffic shaping mechanisms are described as well as traffic shaping in Linux using program tc. It also introduces a design and implementation of traffic shaper using DPDK framework for networks with 10 Gbps bandwidth. The traffic shaper uses a complex mechanism of hierarchical token bucket. The system was tested using high speed traffic generator Spirent.

Racionalizace obrábění řetězových článků do nástrojových zásobníků obráběcích strojů ve firmě Kovo Děčín, s.r.o.

katedra: KOM

Influence of Processing Techniques on Microstructure and Mechanical Properties of a Biodegradable Mg-3Zn-2Ca Alloy

New Mg-3Zn-2Ca magnesium alloy was prepared using different processing techniques: gravity casting as well as squeeze casting in liquid and semisolid states. Materials were further thermally treated; thermal treatment of the gravity cast alloy was additionally combined with the equal channel angular pressing (ECAP). Alloy processed by the squeeze casting in liquid as well as in semisolid state exhibit improved plasticity; the ECAP processing positively influenced both the tensile and compressive characteristics of the alloy. Applied heat treatment influenced the distribution and chemical composition of present intermetallic phases. Influence of particular processing techniques, heat treatment, and intermetallic phase distribution is thoroughly discussed in relation to mechanical behavior of presented alloys.New Mg-3Zn-2Ca magnesium alloy was prepared using different processing techniques: gravity casting as well as squeeze casting in liquid and semisolid states. Materials were further thermally treated; thermal treatment of the gravity cast alloy was additionally combined with the equal channel angular pressing (ECAP). Alloy processed by the squeeze casting in liquid as well as in semisolid state exhibit improved plasticity; the ECAP processing positively influenced both the tensile and compressive characteristics of the alloy. Applied heat treatment influenced the distribution and chemical composition of present intermetallic phases. Influence of particular processing techniques, heat treatment, and intermetallic phase distribution is thoroughly discussed in relation to mechanical behavior of presented alloys

Evolution of mitochondrial TAT translocases illustrates the loss of bacterial protein transport machines in mitochondria

Abstract Background Bacteria and mitochondria contain translocases that function to transport proteins across or insert proteins into their inner and outer membranes. Extant mitochondria retain some bacterial-derived translocases but have lost others. While BamA and YidC were integrated into general mitochondrial protein transport pathways (as Sam50 and Oxa1), the inner membrane TAT translocase, which uniquely transports folded proteins across the membrane, was retained sporadically across the eukaryote tree. Results We have identified mitochondrial TAT machinery in diverse eukaryotic lineages and define three different types of eukaryote-encoded TatABC-derived machineries (TatAC, TatBC and TatC-only). Here, we investigate TatAC and TatC-only machineries, which have not been studied previously. We show that mitochondria-encoded TatAC of the jakobid Andalucia godoyi represent the minimal functional pathway capable of substituting for the Escherichia coli TatABC complex and can transport at least one substrate. However, selected TatC-only machineries, from multiple eukaryotic lineages, were not capable of supporting the translocation of this substrate across the bacterial membrane. Despite the multiple losses of the TatC gene from the mitochondrial genome, the gene was never transferred to the cell nucleus. Although the major constraint preventing nuclear transfer of mitochondrial TatC is likely its high hydrophobicity, we show that in chloroplasts, such transfer of TatC was made possible due to modifications of the first transmembrane domain. Conclusions At its origin, mitochondria inherited three inner membrane translocases Sec, TAT and Oxa1 (YidC) from its bacterial ancestor. Our work shows for the first time that mitochondrial TAT has likely retained its unique function of transporting folded proteins at least in those few eukaryotes with TatA and TatC subunits encoded in the mitochondrial genome. However, mitochondria, in contrast to chloroplasts, abandoned the machinery multiple times in evolution. The overall lower hydrophobicity of the Oxa1 protein was likely the main reason why this translocase was nearly universally retained in mitochondrial biogenesis pathways

The effect of biological and chemical additives on the chemical composition and fermentation process of Dactylis glomerata silage

This study was carried out to determine the chemical composition, silage quality and ensilability of ten cocksfoot cultivars using biological and chemical silage additives. The plant material was harvested from the first and second cut, cultivated at the Research Station of Fodder Crops in Vatín, Czech Republic. Wilted forage was chopped and ensiled in mini-silos with 3 replicates per treatment. The treatments were: 1) without additives, used as a control; 2) with bacterial inoculants; and 3) with chemical preservatives. The results indicated that the year factor (2012-2013) influenced significantly the chemical composition of the silage in both cuts. The use of biological inoculants reduced the content of crude fibre and acid detergent fibre; but it did not influence the content of neutral detergent fibre, in comparison with the control silage in both cuts. Furthermore, the application of biological inoculants reduced the concentration of lactic acid (LA) and acetic acid (AA) in contrast to the control silage in the first cut. Moreover, in the second cut the same values tended to be the opposite. Interestingly, ‘Amera’ was the unique variety that presented a high concentration of butyric acid (0.2%) in comparison with other varieties in the first cut. In conclusion, the biological inoculants had a favourable effect on silage fermentation. Notably, only ‘Greenly’ and ‘Starly’ varieties from the first cut; and ‘Greenly’, ‘Sw-Luxor’, and ‘Otello’ varieties from the second cut were appropriate for ensiling because their pH-values; LA and AA concentrations were ideal according to the parameters of the fermentation process

Influence of processing techniques on microstructure and mechanical properties of a biodegradable Mg-3Zn-2Ca alloy

New Mg-3Zn-2Ca magnesium alloy was prepared using different processing techniques: gravity casting as well as squeeze casting in liquid and semisolid states. Materials were further thermally treated; thermal treatment of the gravity cast alloy was additionally combined with the equal channel angular pressing (ECAP). Alloy processed by the squeeze casting in liquid as well as in semisolid state exhibit improved plasticity; the ECAP processing positively influenced both the tensile and compressive characteristics of the alloy. Applied heat treatment influenced the distribution and chemical composition of present intermetallic phases. Influence of particular processing techniques, heat treatment, and intermetallic phase distribution is thoroughly discussed in relation to mechanical behavior of presented alloys.Web of Science911art. no. 88