Biological aspects of the leafminer Liriomyza sativae (Diptera: Agromyzidae) on melon (Cucumis melo L.)

A mosca-minadora Liriomyza sativae Blanchard é uma praga importante em cultivos de meloeiro (Cucumis melo L.) no Brasil. No entanto, as suas informações sobre biologia, em meloeiro, são escassas. Portanto, o objetivo deste trabalho foi conhecer alguns aspectos biológicos dessa praga, criada em plantas de meloeiro, em condições de laboratório a 25°C. As informações obtidas mostram que o ciclo biológico de L. sativae é de 15,9±0,04 dias (ovo-adulto), sendo: ovo (2,7±0,01 dias), larva (4,1±0,03 dias) e pupa (9,1±0,03 dias). A razão sexual é de 0,51 e as fêmeas vivem mais tempo (19,3±1,09 dias) que os machos (16,2±0,96 dias). Essas informações podem auxiliar na adoção de medidas de manejo integrado de L. sativae em cultivos de meloeiro e melhorar os sistemas de criação da mosca minadora em laboratório.The leafminer Liriomyza sativae Blanchard is an important insect pest on melon crops in Brazil. However, the information about its biology on melon (Cucumis melo L.) is scarce. Therefore, the aim of this research was to know some biological aspects of this pest, reared on melon plants, under laboratory conditions at 25°C. Our results showed that the biological cycle of L. sativae lasts 15.9±0.04 days (egg-adult), namely: egg (2.7±0.01 days), larva (4.1±0.03 days) and pupa (9.1±0.03 days). The sex ratio is 0.51 and the females live (19.3±1.09 days) longer than males (16.2±0.96 days). These results can help in the integrated management of L. sativae on melon crops and improve the systems for rearing this leafminer in laboratory.Universidade Federal Rural do Semi-Árido (UFERSA) Departamento de Ciências VegetaisUniversidade Estadual Paulista (UNESP) Faculdade de Ciências Agrárias e Veterinárias (FCAV)Universidade Federal de Lavras (UFLA) Departamento de EntomologiaUniversidade Estadual Paulista (UNESP) Faculdade de Ciências Agrárias e Veterinárias (FCAV

Hsa-miR-155-5p Up-Regulation in Breast Cancer and Its Relevance for Treatment With Poly[ADP-Ribose] Polymerase 1 (PARP-1) Inhibitors

miR-155-5p is a well-known oncogenic microRNA, showing frequent overexpression in human malignancies, including breast cancer. Here, we show that high miR-155-5p levels are associated with unfavorable prognostic factors in two independent breast cancer cohorts (CSS cohort, n = 283; and TCGA-BRCA dataset, n = 1,095). Consistently, miR-155-5p results as differentially expressed in the breast cancer subgroups identified by the surrogate molecular classification in the CSS cohort and the PAM50 classifier in TCGA-BRCA dataset, with the TNBC and HER2-amplified tumors carrying the highest levels. Since the analysis of TCGA-BC dataset also demonstrated a significant association between miR-155-5p levels and the presence of mutations in homologous recombination (HR) genes, we hypothesized that miR-155-5p might affect cell response to the PARP-1 inhibitor Olaparib. As expected, miR-155-5p ectopic overexpression followed by Olaparib administration resulted in a greater reduction of cell viability as compared to Olaparib administration alone, suggesting that miR-155-5p might induce a synthetic lethal effect in cancer cells when coupled with PARP-1-inhibition. Overall, our data point to a role of miR-155-5p in homologous recombination deficiency and suggest miR-155-5p might be useful in predicting response to PARP1 inhibitors in the clinical setting

ANALYSIS OF THE IMPACT OF ABAMECTIN ON <i>LIRIOMYZA TRIFOLII</i> (BURGESS) (DIPTERA: AGROMYZIDAE)

AbstractThe effect of abamectin on the leafminer, Liromyza trifolii (Burgess), was investigated using topical applications, leaf dips, and repellency bioassays. Topical application of technical material in acetone to susceptible adult females provides an LD50 value of 0.404 ppm. Leaf-dip assays using abamectin formulated in water with third-instar larvae gave an LC50 value of 0.386 ppm. These data provide baseline information on the toxicity of this material to L. trifolii and are important in the development of resistance management programs. Adult female leaf miners were repelled from feeding and ovipositing on chrysanthemum plants treated with formulated abamectin up to 7 days after foliar application in choice and no-choice assays. The implications of these data in managing populations of L. trifolii are discussed.</jats:p

Phytotoxicity on Poinsettia, California, 1979

Abstract Tests were carried out at the Paul Ecke Poinsettia Ranch, Encinitas during Oct 16-Dec 4. The following materials and rates were evaluated: Resmethrin 2E (SPB-1382), Permethrin 2E (SBP-1513) and 3.2E (Pounce) (Permanone 40) (all at 0.1 lb ai/100 gal) and Bendiocarb 76W (NC 6897) (0.25 lb ai/100 gal). A spreader-sticker, X-77, was added at 1 ml/gal to tfie finished spray. Materials were applied to both sides of leaves and bracts using a 10 gal hydro-picton pump delivering 2.5 gpm (9.5 litres per min) at 100 psi. The C-l red, white and Bl varieties were used with 4 replicates/variety/treatment with 3 five inch pots per replicate. Applications were made on 3 dates: Oct 16, when plants were well-rooted (ca 3-4 weeks after sticking); Nov 6, when bracts begin to show color and flower buds were evident; Nov 27, when plants were 0.5-0.75 in full bloom. Temperature range at the time of the 1st, 2nd and 3rd applications were 20.0-23.3, 21.1-23.9 and 21.7-26.7° C, respectively. Observations of phytotoxicity were made 2, 4, and 7 days after each spray application and compared to an untreated check.</jats:p

Nursery Potted Vegetable Phytotoxicity Evaluations, California, 1979-80

Abstract Phytotoxicity resulting from one application of selected insecticides (one insecticide/vegetable) to several potted vegetables was determined. The following materials were evaluated: Lannate 1.8L, Orthene 75S, Pirimor 50W, Pramex 2E, SPB-1382 2E, Vydate 2L. Compounds were chosen based on their ability to control many pests commonly found on potted vegetables and due to a lack of information necessary for their labeling on these crops in a nursery. Applications were made in Jan, March, July or Aug and several plots designs were employed (Table 1). Materials were applied until run-off with a hand gun operating from a 2.5 gpm (9.5 litres per min) piston pump at 125 psi. Plants were sprayed outdoors at all locations and then transferred to raised benches within greenhouses at UCR and onto black plastic sheets located at ground level under shade at cooperating nurseries. The soil at all locations was based on a University of California mixture with a pH of ca 7.4. Following the date of spray application, data on phytotoxicity were recorded weekly for 4 weeks. Damage was evaluated based on the overall appearance of the plants compared to an untreated control: 0-no damage; 1-slight damage, no effect on marketability; 2-moderate damage, marketability reduced; 3-heavy damage, unmarketable. An evaluation of phytoroxic effects was made on plant growth if this occurred after the insecticide applications. Observations on phytotoxicity were made by at least 2 individuals on each date and where possible, only the innermost plants within replicates were evaluated.</jats:p