Natural History of Curtis Island, Bass Strait - 4. The Reptiles of Curtis and Rodondo Islands

Four species of reptiles and details of their ecology are recorded from Curtis Island and three species from Rodondo lsland. Curtis and Rodondo Islands are situated in the deepest part of Bass Strait and were the first islands isolated after the most recent ice age. The paleogeography of the islands is outlined and the origins of the reptile faunas and terrestrial ecosystems are discussed. It is concluded that all four Curtis Island reptile species and two of the three Rodondo Island species are glacial relicts but that the third Rodondo Island species is a post glacial intrusive

Redescription and ecology of the endemic Tasmanian Scincid lizards Leiolopisma microlepidotum and L. Pretiosum

Two species of scincid lizard, both hitherto identified as Leiolopisma pretiosum, occur syntopically on Mt Wellington, near Hobart, Tasmania. One of these is conspecific with the holotype of Moeoa pretiosa O'Shaughnessy, 1874, while the other is conspecific with the holotype of Mocoa microlepidota O'Shaughnessy, 1874, which has been regarded as a synonym of L. pretiosum since 1887. The two species are redescribed as members of an endemic Tasmanian subgroup within the Australian radiation of the genus Leiolopisma (sensu Greer 1982). Leiolopisma mierolepidotum is confined to disjunct alpine populations in the south and west of Tasmania. It differs from L. pretiosum in colour pattern, larger size and more robust, less depressed head and body. Aspects of the biology and ecology of these and other Tasmanian endemic Leiolopisma species are also discussed

A systematic review of outcomes reported inpediatric perioperative research: A report from the Pediatric Perioperative Outcomes Group

The Pediatric Perioperative Outcomes Group (PPOG) is an international collaborative of clinical investigators and clinicians within the subspecialty of pediatric anesthesiology and perioperative care which aims to use COMET (Core Outcomes Measures in Effectiveness Trials) methodology to develop core outcome sets for infants, children, and young people that are tailored to the priorities of the pediatric surgical population. Focusing on four age‐dependent patient subpopulations determined a priori for core outcome set development: (a) neonates and former preterm infants (up to 60 weeks postmenstrual age); (b) infants (>60 weeks postmenstrual age—1‐13‐<18 years), we conducted a systematic review of outcomes reported in perioperative studies that include participants within age‐dependent pediatric subpopulations. Our review of pediatric perioperative controlled trials published from 2008 to 2018 identified 724 articles reporting 3192 outcome measures. The proportion of published trials and the most frequently reported outcomes varied across predetermined age‐groups. Outcomes related to patient comfort, particularly pain and analgesic requirement, were the most frequent domain for infants, children, and adolescents. Clinical indicators, particularly cardiorespiratory or medication‐related adverse events, were the most common outcomes for neonates and infants <60 weeks and were the second most frequent domain at all other ages. Neonates and infants <60 weeks of age were significantly under‐represented in perioperative trials. Patient‐centered outcomes, healthcare utilization, and bleeding/transfusion‐related outcomes were less often reported. In most studies, outcomes were measured in the immediate perioperative period, with the duration often restricted to the postanesthesia care unit or the first 24 postoperative hours. The outcomes identified with this systematic review will be combined with patient‐centered outcomes identified through a subsequent stakeholder engagement study to arrive at a core outcome set for each age‐specific group

Сетевая система контроля технологического процесса выращивания полупроводниковых кристаллов и тонких пленок

Экспериментальное моделирование аппаратно-программного обеспечения показало достаточную надежность работы системы и значительное уменьшение трудоемкости контроля и управления параметрами технологического процесса

Emergence of a new norovirus genotype II.4 variant associated with global outbreaks of gastroenteritis

Norovirus (NoV) is highly infectious and is the major cause of outbreak gastroenteritis in adults, with pandemic spread of the virus being reported in 1995 and 2002. The NoV genome is genetically diverse, which has hampered development of sensitive molecular biology-based methods. In this study we report on a nested reverse transcriptase PCR (nRT-PCR) that was designed to amplify the highly conserved 3` end of the polymerase region and the 5` end of the capsid gene of NoV genogroup II (GII). The nRT-PCR was validated with strains isolated from sporadic and outbreak cases between 1997 and 2004 in New South Wales, Australia. Phylogenetic analysis identified six genotypes circulating in New South Wales, GII.1, GII.3, GII.4, GII.6, GII.7, and GII.10, with GII.4 being the predominant genotype. In 2004, there was a marked increase in NoV GII activity in Australia, with a novel GII.4 variant being identified as the etiological agent in 18 outbreaks investigated. This novel GII.4 variant, termed Hunter virus, differed by more than 5% at the amino acid level across the capsid from any other NoV strain in the GenBank and EMBL databases. The Hunter virus was subsequently identified as the etiological agent in large epidemics of gastroenteritis in The Netherlands, Japan, and Taiwan in 2004 and 2005

Recombination within the pandemic norovirus GII.4 lineage.

Norovirus (NoV) is the leading cause of viral gastroenteritis globally. Since 1996, NoV variants of a single genetic lineage, GII.4, have been associated with at least six pandemics of acute gastroenteritis and caused between 62 and 80% of all NoV outbreaks. The emergence of these novel GII.4 variants has been attributed to rapid evolution and antigenic variation in response to herd immunity; however, the contribution of recombination as a mechanism facilitating emergence is increasingly evident. In this study, we sought to examine the role that intragenotype recombination has played in the emergence of GII.4 variants. Using a genome-wide approach including 25 complete genome sequences generated as part of this study, 11 breakpoints were identified within the NoV GII.4 lineage. The breakpoints were located at three recombination hot spots: near the open reading frame 1/2 (ORF1/2) and ORF2/3 overlaps, as well as within ORF2, which encodes the viral capsid, at the junction of the shell and protruding domains. Importantly, we show that recombination contributed to the emergence of the recent pandemic GII.4 variant, New Orleans 2009, and a newly identified GII.4 variant, termed Sydney 2012. Reconstructing the evolutionary history of the GII.4 lineage reveals the widespread impact of both inter- and intragenotype recombination on the emergence of many GII.4 variants. Lastly, this study highlights the many challenges in the identification of true recombination events and proposes that guidelines be applied for identifying NoV recombinants

Recombinant GII.P16/GII.4 sydney 2012 was the dominant norovirus identified in Australia and New Zealand in 2017

For the past two decades, norovirus pandemic variants have emerged every 3–5 years, and dominate until they are replaced by alternate strains. However, this scenario changed in 2016 with the co-circulation of six prevalent viruses, three of which possessed the pandemic GII.4 Sydney 2012 capsid. An increased number of institutional gastroenteritis outbreaks were reported within the Oceania region in mid-2017. This study identified emerging noroviruses circulating in Australia and New Zealand in 2017 to assess the changing dynamics of the virus infection. RT-PCR-based methods, next generation sequencing, and phylogenetic analyses were used to genotype noroviruses from both clinical and wastewater samples. Antigenic changes were observed between the capsid of pandemic Sydney 2012 variant and the two new Sydney recombinant viruses. The combination of these antigenic changes and the acquisition of a new ORF1 through recombination could both facilitate their ongoing persistence in the population. Overall, an increased prevalence of GII.P16/GII.4 Sydney 2012 viruses was observed in 2017, replacing the GII.P16/GII.2 recombinant that dominated in the region at the end of 2016. This shift in strain dominance was also observed in wastewater samples, demonstrating the reliability of wastewater as a molecular surveillance tool

Contribution of intra- and interhost dynamics to norovirus evolution

Norovirus (NoV) is an emerging RNA virus that has been associated with global epidemics of gastroenteritis. Each global epidemicarises with the emergence of novel antigenic variants. While the majority of NoV infections are mild and self-limiting, inthe young, elderly, and immunocompromised, severe and prolonged illness can result. As yet, there is no vaccine or therapeutictreatment to prevent or control infection. In order to design effective control strategies, it is important to understand the mechanismsand source of the new antigenic variants. In this study, we used next-generation sequencing (NGS) technology to investigategenetic diversification in three contexts: the impact of a NoV transmission event on viral diversity and the contribution todiversity of intrahost evolution over both a short period of time (10 days), in accordance with a typical acute NoV infection, anda prolonged period of time (288 days), as observed for NoV chronic infections of immunocompromised individuals. Investigationsof the transmission event revealed that minor variants at frequencies as low as 0.01% were successfully transmitted, indicatingthat transmission is an important source of diversity at the interhost level of NoV evolution. Our results also suggest thatchronically infected immunocompromised subjects represent a potential reservoir for the emergence of new viral variants. Incontrast, in a typical acute NoV infection, the viral population was highly homogenous and relatively stable. These results indicate that the evolution of NoV occurs through multiple mechanisms