Effects of Anthocleista Djalonensis (Chev.) methanol root bark extract on some fertility parameters in male rats

Background: Anthocleista djalonensis is a West African plant with several ethnomedicinal uses.Objectives: This study evaluated the effect of Anthocleista djalonensis methanol root bark extract on fertility parameters in male rats.Material and Methods: The rats were randomly allotted to four groups (n=5). Group A (control) received distilled water (10 ml/kg), groups B, C, D received oral doses of 100, 200 and 400 mg/kg/day of the extract respectively for 28 days. The mean body, testicular, and epididymal weights; sperm indices; and serum testosterone level were assessed.Results: There were no significant changes on the body weights of the treated rats but their testicular and epididymal weights increased significantly (P˂0.05) at the dose of 400 mg/kg in comparison with the control group. Sperm indices such as sperm count, motility, morphology and viability increased significantly (P˂0.05) at the doses of 200 and 400 mg/kg/day when compared to the control. There was also a significant (P˂0.05) increase in serum testosterone concentration at the dose of 400 mg/kg/day of the extract.Conclusion: The results suggest that the methanol root bark extract of A. djalonensis improves fertility parameters in male rats thereby justifying its use in ethnomedicine.Keywords: Sperm count, testosterone level, spermatogenesis, oxidative stres

COMPARATIVE ASSESSMENT OF LIPID PROFILE IN PRE-MENOPAUSAL AND MENOPAUSAL WOMEN IN NNEWI NIGERIA

The study was aimed at determining the effect of menopause on lipid profile. A total of 100 apparently healthy subjects who comprised 50 menopausal women aged 45 – 77 years and 50 pre-menopausal women between the aged between 20-52 years were recruited. Ethical approval was obtained from the Faculty of Health Science and Technology, Nnamdi Azikiwe University ethics committee and informed consent of each participant was obtained prior to recruitment. We estimated serum levels of total cholesterol using the enzymatic end point method as described by Roeschlau et al., (1974), HDL was performed using the combination of phosphotungstate precipitation and enzymatic method as described by Burstein et al., (1980), LDL by the combination of polyvinyl sulphate precipitation and enzymatic method of Assman et al., (1984), VLDL was estimated using the method as described by Friedwald et al., (1972) and triglycerides by the enzymatic method as described by Tietz (1990). The results shows that the mean levels of serum triglycerides (TG) and low density lipoprotein cholesterol (LDL cholesterol) showed a statistically significant increase in menopausal women compared with the premenopausal subjects (P<0.05) while there was no significant difference in the mean values of serum high density lipoprotein cholesterol (HDL cholesterol), very low density lipoprotein cholesterol (VLDL cholesterol) and total cholesterol (P>0.05). There was a progressive increase in the mean levels of total cholesterol, triglycerides, LDL-cholesterol and VLDLcholesterol with duration of menopause while the levels of HDL-cholesterol decreased with duration of menopause. There were no significant variations among the various age categories of the menopausal subjects and the levels of the lipid parameters studied (p>0.05). The findings suggest that premenopausal women have less proatherogenic lipid profile than their menopausal counterparts

COMPARATIVE ASSESSMENT OF LIPID PROFILE IN PRE-MENOPAUSAL AND MENOPAUSAL WOMEN IN NNEWI NIGERIA

The study was aimed at determining the effect of menopause on lipid profile. A total of 100 apparently healthy subjects who comprised 50 menopausal women aged 45 – 77 years and 50 pre-menopausal women between the aged between 20-52 years were recruited. Ethical approval was obtained from the Faculty of Health Science and Technology, Nnamdi Azikiwe University ethics committee and informed consent of each participant was obtained prior to recruitment. We estimated serum levels of total cholesterol using the enzymatic end point method as described by Roeschlau et al., (1974), HDL was performed using the combination of phosphotungstate precipitation and enzymatic method as described by Burstein et al., (1980), LDL by the combination of polyvinyl sulphate precipitation and enzymatic method of Assman et al., (1984), VLDL was estimated using the method as described by Friedwald et al., (1972) and triglycerides by the enzymatic method as described by Tietz (1990). The results shows that the mean levels of serum triglycerides (TG) and low density lipoprotein cholesterol (LDL cholesterol) showed a statistically significant increase in menopausal women compared with the premenopausal subjects (P<0.05) while there was no significant difference in the mean values of serum high density lipoprotein cholesterol (HDL cholesterol), very low density lipoprotein cholesterol (VLDL cholesterol) and total cholesterol (P>0.05). There was a progressive increase in the mean levels of total cholesterol, triglycerides, LDL-cholesterol and VLDLcholesterol with duration of menopause while the levels of HDL-cholesterol decreased with duration of menopause. There were no significant variations among the various age categories of the menopausal subjects and the levels of the lipid parameters studied (p>0.05). The findings suggest that premenopausal women have less proatherogenic lipid profile than their menopausal counterparts

Assessment Of Tumour Necrosis Factor-Alpha (Tnf- Α) And Creatinine Levels In Echis Ocellatus Bite Victims In Jos Metropolis, Nigeria

This study was designed to assess tumour necrosis factor-alpha and creatinine levels in Echis ocellatus bite victims. A total of 50 subjects were recruited. Out of this number, 40 were victims of E. ocellatus bite and the remaining 10 were non-victims of snake bite who served as the control group. Blood samples were collected from the victims within 24 hours of the snake bite and EchiTAb-G antivenom administered within the same period. Another batch of blood sample was collected 48 hours post-administration of the anti-venom. Tumour necrosis factor-alpha (TNF-alpha) levels were estimated by the Enzyme Linked Immunosorbent Assay technique while creatinine levels were determined using kinetic-spectrophotometric procedure. The mean serum levels of tumour necrosis factor-alpha and creatinine were significantly increased in E. ocellatus bite victims compared with the control group (P<0.05). Furthermore, the mean serum level of TNFalpha was significantly lower in E. ocellatus bite victims, post-administration of anti-venom, compared with the pre-administration of anti-venom (P<0.05). In contrast, no significant difference was observed in the mean serum level of creatinine in E. ocellatus bite victims, post-administration of anti-venom, compared with the pre-administration of anti-venom (P>0.05). Moreover, the mean serum level of creatinine was found to be significantly increased in E. ocellatus bite victims, post-administration of anti-venom, compared with the control group (P<0.05), while no significant difference was observed in the mean serum level of tumour necrosis factor-alpha in E. ocellatus bite victims, post-administration of anti-venom, compared with the control group(P>0.05). A positive correlation existed between tumour necrosis factor-alpha and creatinine levels in E. ocellatus bite subjects (r= 0.782). Echis ocellatus bite is a risk factor for renal damage indicated by an elevated serum creatinine, thus health authorities should make EchiTAb-G anti-venom freely available in health facilities and administered as quickly as possible to reduce the risk of renal damage in Echis ocellatus bite-prone areas

The practice of hepatocellular cancer surveillance in Nigeria

Background: Hepatocellular cancer is a disease of global and public health importance due to the widespread distribution of risk factors and associated high case fatality. Hepatocellular Cancer (HCC) in Sub-Saharan Africa is commonly seen among the younger age groups (<45 years) who present mostly in the terminal stage, when the disease is not amenable to any curative therapy. Hepatocellular Carcinoma surveillance employs the use of simple, cheap and readily available investigations, to detect early curable cancer in individuals with risk factors for HCC.Objectives:The aim of this study is to assess the practice of hepatocellular cancer screening among physicians.Methodolgy:This is a nationwide online survey carried out among physicians who care for patients with HCC. A questionnaire was sent out via a web link to all consenting doctors in Nigeria. The responses were collated in a cloud-based application and data was analysed using Epi-info version 20.Results:Atotal of 218 respondents, 142 were males (65.1 %) with a mean age of 37.6 ± 5.7 years. The modal age group was 31-40 years 153 (69.5%). The main factors considered as a hindrance to surveillance were; the cost of the tests (57.7%), failure of return of patients (50.5%) and not being aware of a surveillance program (45.2 %). The majority of the respondents were Gastroenterologists and Family Physicians. 54% of the gastroenterologists and 64% of the family physicians have never offered HCC surveillance to their patients.Conclusion:This survey highlights a knowledge gap in HCC surveillance among physicians. There is a need to make HCCsurveillance a daily routine among patients at risk by all physicians. Keywords: Surveillance, Hepatocellular Carcinoma, HBV, HCV, Cancer screening