Anticonvulsant and proconvulsant roles of nitric oxide in experimental epilepsy models

The effect of acute (120 mg/kg) and chronic (25 mg/kg, twice a day, for 4 days) intraperitonial injection of the nitric oxide (NO) synthase (NOS) inhibitor NG-nitro-L-arginine (L-NOARG) was evaluated on seizure induction by drugs such as pilocarpine and pentylenetetrazole (PTZ) and by sound stimulation of audiogenic seizure-resistant (R) and audiogenic seizure-susceptible (S) rats. Seizures were elicited by a subconvulsant dose of pilocarpine (100 mg/kg) only after NOS inhibition. NOS inhibition also simultaneously potentiated the severity of PTZ-induced limbic seizures (60 mg/kg) and protected against PTZ-induced tonic seizures (80 mg/kg). The audiogenic seizure susceptibility of S or R rats did not change after similar treatments. In conclusion, proconvulsant effects of NOS inhibition are suggested to occur in the pilocarpine model and in the limbic components of PTZ-induced seizures, while an anticonvulsant role is suggested for the tonic seizures induced by higher doses of PTZ, revealing inhibitor-specific interactions with convulsant dose and also confirming the hypothesis that the effects of NOS inhibitors vary with the model of seizur

Investigation on the growth, characterization and computational analysis of 2-Amino-2-thiazoline single crystals

By adapting the slow evaporation techniquethe titled compound 2-Amino-2-thiazoline (AMTZ) has been synthesized. The structural parameters of the crystal was studied by using single crystal X-ray diffraction method.Without the deposition of secondary phases, crystallite nature of the sample has been confirmed by analyzing powder X-ray diffraction method. From UV–visible spectrum study, the cut off wavelength was perceived at 285 nm & its optical constants has been calculated.Up to 128 °C, the thermal constancy of the sample was maintained & it reveals the durability of the crystal. The scanning electron microscope investigation has been carried out to decisive the surface morphology of the synthesized crystal. The mechanical roughness of the crystal has been characterized by Vickers's micro hardness test. It has been reported, that the variations in dielectric constant and loss for frequencies and temperature were observed for AMTZ crystals. By adapting Kurtz Perry Powder technique the second harmonic generation (SHG) efficiency was confirmed. By utilizing09W program package, B3LYP/6–311++G(d, p) method was adopted &the optimization of the structure was carried outto calculate the donor accepter interaction energy.The Time-Dependent Density Functional Theory (TD-DFT) was used to compute the electronic level of transition

A massively parallel barcoded sequencing pipeline enables generation of the first ORFeome and interactome map for rice.

Systematic mappings of protein interactome networks have provided invaluable functional information for numerous model organisms. Here we develop PCR-mediated Linkage of barcoded Adapters To nucleic acid Elements for sequencing (PLATE-seq) that serves as a general tool to rapidly sequence thousands of DNA elements. We validate its utility by generating the ORFeome for Oryza sativa covering 2,300 genes and constructing a high-quality protein-protein interactome map consisting of 322 interactions between 289 proteins, expanding the known interactions in rice by roughly 50%. Our work paves the way for high-throughput profiling of protein-protein interactions in a wide range of organisms