20 research outputs found
A New and Simple TRG Multiplex PCR Assay for Assessment of T-cell Clonality: A Comparative Study from the EuroClonality Consortium
T-cell Receptor Gamma (TRG) rearrangements are commonly used to detect clonal lymphoproliferations in hematopathology, since
they are rearranged in virtually all T lymphocytes and have a relatively limited recombinatorial repertoire, which reduces the risk of false
negative results, at the cost of potential false positivity. We developed an initial one-tube, 2-fluorochrome EuroClonality TRG PCR
multiplex (TRG-1T-2F) which was compared to the original 2-tube, 2-fluorochrome EuroClonality/BIOMED-2 TRG PCR (TRG-2T-2F)
and a commercial Invivoscribe one-tube, one-fluorochrome kit (IVS-1T-1F) on a series of 239 samples, including both T-cell
malignancies and reactive cases. This initial assay yielded discrepant results between the 10 participating EuroClonality laboratories
when using 2 fluorochromes, leading to adoption of a final single color EuroClonality strategy (TRG-1T-1F). Compared to TRG-2T-2F,
both TRG-1T-1F and IVS-1T-1F demonstrated easier interpretation and a lower risk of false positive from minor peaks in dispersed
repertoires. Both generate smaller fragments and as such are likely to be better adapted to analysis of formalin-fixed paraffinembedded (FFPE) tissue samples. Their differential performance was mainly explained by (i)
Standardized next-generation sequencing of immunoglobulin and T-cell receptor gene recombinations for MRD marker identification in acute lymphoblastic leukaemia; a EuroClonality-NGS validation study
Amplicon-based next-generation sequencing (NGS) of immunoglobulin (IG) and T-cell receptor (TR) gene rearrangements
for clonality assessment, marker identification and quantification of minimal residual disease (MRD) in lymphoid neoplasms
has been the focus of intense research, development and application. However, standardization and validation in a
scientifically controlled multicentre setting is still lacking. Therefore, IG/TR assay development and design, including
bioinformatics, was performed within the EuroClonality-NGS working group and validated for MRD marker identification
in acute lymphoblastic leukaemia (ALL). Five EuroMRD ALL reference laboratories performed IG/TR NGS in 50
diagnostic ALL samples, and compared results with those generated through routine IG/TR Sanger sequencing. A central
polytarget quality control (cPT-QC) was used to monitor primer performance, and a central in-tube quality control (cIT-QC)
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Capillary electrophoresis single-strand conformation analysis (CE-SSCA) for clonality detection in lymphoproliferative disorders
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Receptor kinase profiles identify a rationale for multi-target kinase inhibition in immature T-ALL.
International audienc
Receptor kinase profiles identify a rationale for multi-target kinase inhibition in immature T-ALL.
International audienc
Clinical impact of NOTCHI and/or FBXW7 mutations, FLASH deletion and TCR status in pediatric T-cell lymphoblastic lymphoma.
International audienc
TCRα rearrangements identify a subgroup of NKL-deregulated adult T-ALLs associated with favorable outcome
International audienceno abstrac