In-situ Conservation of wild forest coffee-Exploring the potential of participatory forest management in south west Ethiopia

Maintaining the gene pool of Coffea arabica in the forests of South West Ethiopia, where this plant evolved and has its genetic hearth, is a challenge of global importance. Participatory Forest Management (PFM) and Biosphere Reserves (BR) are among the various initiatives being tested to maintain this forest and its biodiversity that includes major stands of wild Arabica coffee. This paper makes a comparative analysis of PFM and BR approaches to conservation as applied in Ethiopia. While BR legislation provides a framework for enforcement, there can be difficulties in achieving this on the ground especially with the pressures from communities on these forests and the limited resources of the state to enforce protection. In some cases because core zones are alienated from communities these areas are seen as open access by local communities and have suffered especially from fires and focused deforestation. An alternative approach for in situ conservation of wild coffee using PFM has been explored in South West forest of Ethiopia. PFM, by engaging local communities in the management of forests is believed to increase economic and environmental benefits while reducing costs of conservation..Monthly field monitoring by communities and annual reviews with the government have turned this forest from open access to community controlled. Critically the development of market links and value chains for forest products is motivating communities to maintain and improve their forest. It is hoped that PFM will create a win-win goal – enhancing the role played by the forest in rural development and ensuring the conservation of wild coffee genetic resources

Evidence that the 36kb plasmid of Brachyspira hyodysenteriae contributes to virulence

Swine dysentery (SD) results from infection of the porcine large intestine with the anaerobic intestinal spirochaete Brachyspira hyodysenteriae. Recently the genome of virulent Australian B. hyodysenteriae strain WA1 was sequenced, and a 36. kilobase (kb) circular plasmid was identified. The plasmid contained 31 genes including six rfb genes that were predicted to be involved with rhamnose biosynthesis, and others associated with glycosylation. In the current study a set of PCRs was developed to amplify portions of nine of the plasmid genes. When used with DNA extracted from virulent strain B204, PCR products were generated, but no products were generated with DNA from avirulent strain A1. Analysis of the DNA using pulsed field gel electrophoresis (PFGE) identified a plasmid band in strains WA1 and B204, but not in strain A1. These results demonstrate that strain A1 does not contain the plasmid, and suggests that lack of the plasmid may explain why this strain is avirulent. To determine how commonly strains lacking plasmids occur, DNA was extracted from 264 Australian field isolates of B. hyodysenteriae and subjected to PCRs for three of the plasmid genes. Only one isolate (WA400) that lacked the plasmid was identified, and this absence was confirmed by PFGE analysis of DNA from the isolate and further PCR testing. To assess its virulence, 24 pigs were experimentally challenged with cultures of WA400, and 12 control pigs were challenged with virulent strain WA1 under the same conditions. Significantly fewer (P= 0.03) of the pigs challenged with WA400 became colonised and developed SD (13/24; 54%) compared to the pigs infected with WA1 (11/12; 92%). Gross lesions in the pigs colonised with WA400 tended to be less extensive than those in pigs colonised with WA1, although there were no obvious differences at the microscopic level. The results support the likelihood that plasmid-encoded genes of B. hyodysenteriae are involved in colonisation and/or disease expression

Dealing with mobility: Understanding access anytime, anywhere

The rapid and accelerating move towards the adoption and use of mobile technologies has increasingly provided people and organisations with the ability to work away from the office and on the move. The new ways of working afforded by these technologies are often characterised in terms of access to information and people ‘anytime, anywhere’. This paper presents a study of mobile workers that highlights different facets of access to remote people and information, and different facets of anytime, anywhere. Four key factors in mobile work are identified from the study: the role of planning, working in ‘dead time’, accessing remote technological and informational resources, and monitoring the activities of remote colleagues. By reflecting on these issues, we can better understand the role of technology and artefact use in mobile work and identify the opportunities for the development of appropriate technological solutions to support mobile workers

A method of solving y

An alternative method is shown for solving the differential equation y(k)−f(x)y=0 by means of series. Also included is a result for a sequence of functions {Sn(x)}n=1∞ which gives conditions under which limn(dkdxkSn(x))=dkdxk(limnSn(x))

Working memory capacity and surgical performance whilst exposed to mild hypoxic hypoxaemia (3000m)

INTRODUCTION: Medical Emergency Response Team (MERT) helicopters fly at altitudes of 3,000m in Afghanistan (9,843ft). Civilian hospitals and disaster-relief surgical teams may have to operate at such altitudes or even higher. Mild hypoxia has been seen to affect the performance of novel tasks at flight levels as low as 5,000ft. Aeromedical teams frequently work in unpressurised environments; it is important to understand the implications of this mild hypoxia and investigate whether supplementary oxygen systems are required for some or all of the team members. METHODS: Ten UK orthopaedic surgeons were recruited and in a double blind randomised experimental protocol, were acutely exposed for 45 minutes to normobaric hypoxia (fraction of inspired oxygen (FiO2) ~14.1% - equivalent to 3000m/10,000ft) or normobaric normoxia (sea-level). Basic physiological parameters were recorded. Subjects completed validated tests of verbal working memory capacity (VWMC) and also applied an orthopaedic external fixator (Hoffmann® 3, Stryker UK) to a plastic tibia under test conditions. RESULTS: Significant hypoxia was induced with the reduction of FiO2 to ~14.1% (SpO2 87% vs. 98%). No effect of hypoxia on VWMC was observed. The pin-divergence score (a measure of frame asymmetry) was significantly greater in hypoxic conditions (4.6mm) compared to sea level (3.0mm), there was no significant difference in the penetrance depth (16.9 vs. 17.2mm). One frame would have failed early. DISCUSSION: We believe that surgery at an altitude of 3000m when unacclimated individuals are acutely exposed to atmospheric hypoxia for 45 minutes, can likely take place without supplemental oxygen use but further work is required