385 research outputs found
Seminario di Linguistica e Didattica delle Lingue. Scritti in onore degli ottantâanni di Giovanni Freddi
Include, di Balboni: âLa glottodidattica veneziana: una âscuolaâ?â, pp. 19-54
The antagonism of the prokineticin system counteracts bortezomib induced side effects: Focus on mood alterations
The development of neuropathy and of mood alterations is frequent after chemotherapy. These complications, independent from the antitumoral mechanism, are interconnected due to an overlapping in their processing pathways and a common neuroinflammatory condition. This study aims to verify whether in mice the treatment with the proteasome inhibitor bortezomib (BTZ), at a protocol capable of inducing painful neuropathy, is associated with anxiety, depression and supraspinal neuroinflammation. We also verify if the therapeutic treatment with the antagonist of the prokineticin (PK) system PC1, which is known to contrast pain and neuroinflammation, can prevent mood alterations. Mice were treated with BTZ (0.4 mg/kg three times/week for 4 weeks); mechanical allodynia and locomotor activity were evaluated over time while anxiety (dark light and marble burying test), depression (sucrose preference and swimming test) and supraspinal neuroinflammation were checked at the end of the protocol. BTZ treated neuropathic mice develop anxiety and depression. The presence of mood alterations is related to the presence of neuroinflammation and PK system activation in prefrontal cortex, hippocampus and hypothalamus with high levels of PK2 and PKR2 receptor, ILâ6 and TNFâα, TLR4 and an upregulation of glial markers. PC1 treatment, counteracting pain, prevented the development of supraspinal inflammation and depressionâlike behavior in BTZ mice
Single-cell gene network analysis and transcriptional landscape of MYCN-amplified neuroblastoma cell lines
Neuroblastoma (NBL) is a pediatric cancer responsible for more than 15% of cancer deaths in children, with 800 new cases each year in the United States alone. Genomic amplification of the MYC oncogene family member MYCN characterizes a subset of high-risk pediatric neuroblastomas. Several cellular models have been implemented to study this disease over the years. Two of these, SK-N-BE-2-C (BE2C) and Kelly, are amongst the most used worldwide as models of MYCN-Amplified human NBL. Here, we provide a transcriptome-wide quantitative measurement of gene expression and transcriptional network activity in BE2C and Kelly cell lines at an unprecedented single-cell resolution. We obtained 1105 Kelly and 962 BE2C unsynchronized cells, with an average number of mapped reads/cell of roughly 38,000. The single-cell data recapitulate gene expression signatures previously generated from bulk RNA-Seq. We highlight low variance for commonly used housekeeping genes between different cells (ACTB, B2M and GAPDH), while showing higher than expected variance for metallothionein transcripts in Kelly cells. The high number of samples, despite the relatively low read coverage of single cells, allowed for robust pathway enrichment analysis and master regulator analysis (MRA), both of which highlight the more mesenchymal nature of BE2C cells as compared to Kelly cells, and the upregulation of TWIST1 and DNAJC1 transcriptional networks. We further defined master regulators at the single cell level and showed that MYCN is not constantly active or expressed within Kelly and BE2C cells, independently of cell cycle phase. The dataset, alongside a detailed and commented programming protocol to analyze it, is fully shared and reusable
La linguistica educativa tra ricerca e sperimentazione. Scritti in onore di Carmel Mary Coonan
The volume collects the contributions of academics from various Italian universities who have worked with Carmel Mary Coonan throughout her career at Caâ Foscari University. Starting from the themes that have characterised Carmelâs research interests, including Content and Language Integrated Learning (CLIL), professional development
for language teachers and action research practice, the volume opens up to original methodological reflections and new lines of research in the field of educational linguistics. The relevance and variety of the studies here described confirm the lively and
dynamic character of research and experimentation that shape our discipline. By questioning different language learning environments (both inside and outside language classroom), educational contexts (from kindergarten to university and beyond) and
subjects (students as well as teachers), the volume highlights significant gains achieved in the field of language teaching and learning so far. At the same time, however, it makes clear the urge to further boost plurilingualism in language education, a goal that Carmel has tenaciously pursued throughout her whole career
Making GDPR Usable: A Model to Support Usability Evaluations of Privacy
We introduce a new model for evaluating privacy that builds on the criteria
proposed by the EuroPriSe certification scheme by adding usability criteria.
Our model is visually represented through a cube, called Usable Privacy Cube
(or UP Cube), where each of its three axes of variability captures,
respectively: rights of the data subjects, privacy principles, and usable
privacy criteria. We slightly reorganize the criteria of EuroPriSe to fit with
the UP Cube model, i.e., we show how EuroPriSe can be viewed as a combination
of only rights and principles, forming the two axes at the basis of our UP
Cube. In this way we also want to bring out two perspectives on privacy: that
of the data subjects and, respectively, that of the controllers/processors. We
define usable privacy criteria based on usability goals that we have extracted
from the whole text of the General Data Protection Regulation. The criteria are
designed to produce measurements of the level of usability with which the goals
are reached. Precisely, we measure effectiveness, efficiency, and satisfaction,
considering both the objective and the perceived usability outcomes, producing
measures of accuracy and completeness, of resource utilization (e.g., time,
effort, financial), and measures resulting from satisfaction scales. In the
long run, the UP Cube is meant to be the model behind a new certification
methodology capable of evaluating the usability of privacy, to the benefit of
common users. For industries, considering also the usability of privacy would
allow for greater business differentiation, beyond GDPR compliance.Comment: 41 pages, 2 figures, 1 table, and appendixe
Distinct cytogenetic and clinicopathologic features in acute myeloid leukemia after occupational exposure to pesticides and organic solvents
Complete Exact Solution of Diffusion-Limited Coalescence, A + A -> A
Some models of diffusion-limited reaction processes in one dimension lend
themselves to exact analysis. The known approaches yield exact expressions for
a limited number of quantities of interest, such as the particle concentration,
or the distribution of distances between nearest particles. However, a full
characterization of a particle system is only provided by the infinite
hierarchy of multiple-point density correlation functions. We derive an exact
description of the full hierarchy of correlation functions for the
diffusion-limited irreversible coalescence process A + A -> A.Comment: 4 pages, 2 figures (postscript). Typeset with Revte
Coarsening dynamics of adsorption processes with diffusional relaxation
We investigate the late coarsening stages of one dimensional adsorption
processes with diffusional relaxation. The nonequilibrium domain size
distribution is studied by means of the field theory associated to the
stochastic evolution. An exact asymptotic solution satisfying dynamical scaling
is given for cluster sizes smaller than the average domain length. Our results
are supported and compared with Monte Carlo simulations.Comment: 5 pages, 1 Postscript figur
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