746 research outputs found
Complexation of Cm(III) with the recombinant N-lobe of human serum transferrin studied by time-resolved laser fluorescence spectroscopy (TRLFS)
The complexation of Cm(III) with the recombinant N-lobe of human serum transferrin (hTf/2N) is investigated in the pH range from 4.0 to 11.0 using TRLFS. At pH [greater-than-or-equal] 7.4 a Cm(III) hTf/2N species is formed with Cm(III) bound at the Fe(III) binding site. The results are compared with Cm(III) transferrin interaction at the C-lobe and indicate the similarity of the coordination environment of the C- and N-terminal binding sites with four amino acid residues of the protein, two H2O molecules and three additional ligands (e.g. synergistic anions such as carbonate) in the first coordination sphere. Measurements at c(carbonate)tot = 0.23 mM (ambient carbonate concentration) and c(carbonate)tot = 25 mM (physiological carbonate concentration) show that an increase of the total carbonate concentration suppresses the formation of the Cm(III) hTf/2N species significantly. Additionally, the three Cm(III) carbonate species Cm(CO3)+, Cm(CO3)2- and Cm(CO3)33- are formed successively with increasing pH. In general, carbonate complexation is a competing reaction for both Cm(III) complexation with transferrin and hTf/2N but the effect is significantly higher for the half molecule. At c(carbonate)tot = 0.23 mM the complexation of Cm(III) with transferrin and hTf/2N starts at pH [greater-than-or-equal] 7.4. At physiological carbonate concentration the Cm(III) transferrin species II forms at pH [greater-than-or-equal] 7.0 whereas the Cm(III) hTf/2N species is not formed until pH > 10.0. Hence, our results reveal significant differences in the complexation behavior of the C-terminal site of transferrin and the recombinant N-lobe (hTf/2N) towards trivalent actinides
The ESO Key-Programme ``A Homogeneous Bright QSO Survey'' - I The Methods and the ``Deep'' Fields
This is the first paper in a series aimed at defining a statistically
significant sample of QSOs in the range and .
The selection is carried out using direct plates obtained at the ESO and UK
Schmidt Telescopes, scanned with the COSMOS facility and searched for objects
with an ultraviolet excess. Follow-up spectroscopy, carried out at ESO La
Silla, is used to classify each candidate. In this initial paper, we describe
the scientific objectives of the survey; the selection and observing techniques
used. We present the first sample of 285 QSOs () in a 153 deg
area, covered by the six ``deep'' fields, intended to obtain significant
statistics down with unprecedented photometric accuracy. From
this database, QSO counts are determined in the magnitude range .Comment: 21 pages uuencoded compressed postscript, to appear in Astronomy and
Astrophysics Supplements, 199
The Opacity of Spiral Galaxy Disks VIII: Structure of the Cold ISM
The quantity of dust in a spiral disk can be estimated using the dust's
typical emission or the extinction of a known source. In this paper, we compare
two techniques, one based on emission and one on absorption, applied on
sections of fourteen disk galaxies. The two measurements reflect, respectively
the average and apparent optical depth of a disk section. Hence, they depend
differently on the average number and optical depth of ISM structures in the
disk. The small scale geometry of the cold ISM is critical for accurate models
of the overall energy budget of spiral disks. ISM geometry, relative
contributions of different stellar populations and dust emissivity are all free
parameters in galaxy Spectral Energy Distribution (SED) models; they are also
sometimes degenerate, depending on wavelength coverage. Our aim is to constrain
typical ISM geometry. The apparent optical depth measurement comes from the
number of distant galaxies seen in HST images through the foreground disk. We
measure the IR flux in images from the {\it Spitzer} Infrared Nearby Galaxy
Survey in the same section of the disk that was covered by HST. A physical
model of the dust is fit to the SED to estimate the dust surface density, mean
temperature, and brightness in these disk sections. The surface density is
subsequently converted into the average optical depth estimate. The two
measurements generally agree. The ratios between the measured average and
apparent optical depths of the disk sections imply optically thin clouds in
these disks. Optically thick disks, are likely to have more than a single cloud
along the line-of-sight.Comment: 31 pages, 5 figures, 4 tables, accepted for publication in A
The ESO Slice Project (ESP) galaxy redshift survey: III. The Sample
The ESO Slice Project (ESP) is a galaxy redshift survey extending over about
23 square degrees, in a region near the South Galactic Pole. The survey is ~85%
complete to the limiting magnitude b_J=19.4 and consists of 3342 galaxies with
redshift determination.
The ESP survey is intermediate between shallow, wide angle samples and very
deep, one-dimensional pencil beams; the spanned volume is ~ 5 x 10^4 Mpc^3 at
the sensitivity peak (z ~ 0.1).
In this paper we present the description of the observations and of the data
reduction, the ESP redshift catalogue and the analysis of the quality of the
velocity determinations.Comment: 10 pages, 4 encapsulated figures, uses A&A LATEX; A&A Supplements in
press (June 1998 issue
The ROSAT All-Sky Survey: a Catalog of Clusters of Galaxies in a Region of 1 Ster around the South Galactic Pole
A field of 1.013 ster in the ROSAT all-sky survey (RASS), centered on the
south galactic pole (SGP), has been searched in a systematic, objective manner
for clusters of galaxies. The procedure relied on a correlation of the X-ray
positions and properties of ROSAT sources with the distribution of galaxies in
the COSMOS digitised data base, which was obtained by scanning the plates of
the UK Schmidt IIIa-J optical southern sky survey. The study used the second
ROSAT survey data base (RASS-2) and included several optical observing
campaigns to measure redshifts. The search, a precursor to the larger REFLEX
survey of the whole southern sky, reached the detection limits of the RASS and
the COSMOS data, and yielded a catalog of 186 clusters in which the lowest flux
is 1.5e-12 erg/cm2/s in the 0.1-2.4 keV band. Of these 157 have measured
redshifts. Using a limit of 3.0e-12 erg/cm2/s a complete subset of 112 clusters
was obtained, of which 110 have measured redshifts. The spatial distribution of
the X-ray clusters out to z = 0.15 shows an extension of the Local Supercluster
to the Pisces-Cetus supercluster (z<~0.07), and a more distant orthogonal
structure at 0.07<z<0.15.Comment: To be published in ApJ Supplements in February 2002: 53 pages: 18
figure
Investigation of associations between retinal microvascular parameters and albuminuria in UK Biobank: a cross-sectional case-control study
BACKGROUND: Associations between microvascular variation and chronic kidney disease (CKD) have been reported previously. Non-invasive retinal fundus imaging enables evaluation of the microvascular network and may offer insight to systemic risk associated with CKD. METHODS: Retinal microvascular parameters (fractal dimension [FD] - a measure of the complexity of the vascular network, tortuosity, and retinal arteriolar and venular calibre) were quantified from macula-centred fundus images using the Vessel Assessment and Measurement Platform for Images of the REtina (VAMPIRE) version 3.1 (VAMPIRE group, Universities of Dundee and Edinburgh, Scotland) and assessed for associations with renal damage in a case-control study nested within the multi-centre UK Biobank cohort study. Participants were designated cases or controls based on urinary albumin to creatinine ratio (ACR) thresholds. Participants with ACR ≥ 3 mg/mmol (ACR stages A2-A3) were characterised as cases, and those with an ACR < 3 mg/mmol (ACR stage A1) were categorised as controls. Participants were matched on age, sex and ethnic background. RESULTS: Lower FD (less extensive microvascular branching) was associated with a small increase in odds of albuminuria independent of blood pressure, diabetes and other potential confounding variables (odds ratio [OR] 1.18, 95% confidence interval [CI] 1.03-1.34 for arterioles and OR 1.24, CI 1.05-1.47 for venules). Measures of tortuosity or retinal arteriolar and venular calibre were not significantly associated with ACR. CONCLUSIONS: This study supports previously reported associations between retinal microvascular FD and other metabolic disturbances affecting the systemic vasculature. The association between retinal microvascular FD and albuminuria, independent of diabetes and blood pressure, may represent a useful indicator of systemic vascular damage associated with albuminuria
Spatial and kinematic alignments between central and satellite halos
Based on a cosmological N-body simulation we analyze spatial and kinematic
alignments of satellite halos within six times the virial radius of group size
host halos (Rvir). We measure three different types of spatial alignment: halo
alignment between the orientation of the group central substructure (GCS) and
the distribution of its satellites, radial alignment between the orientation of
a satellite and the direction towards its GCS, and direct alignment between the
orientation of the GCS and that of its satellites. In analogy we use the
directions of satellite velocities and probe three further types of alignment:
the radial velocity alignment between the satellite velocity and connecting
line between satellite and GCS, the halo velocity alignment between the
orientation of the GCS and satellite velocities and the auto velocity alignment
between the satellites orientations and their velocities. We find that
satellites are preferentially located along the major axis of the GCS within at
least 6 Rvir (the range probed here). Furthermore, satellites preferentially
point towards the GCS. The most pronounced signal is detected on small scales
but a detectable signal extends out to 6 Rvir. The direct alignment signal is
weaker, however a systematic trend is visible at distances < 2 Rvir. All
velocity alignments are highly significant on small scales. Our results suggest
that the halo alignment reflects the filamentary large scale structure which
extends far beyond the virial radii of the groups. In contrast, the main
contribution to the radial alignment arises from the adjustment of the
satellite orientations in the group tidal field. The projected data reveal good
agreement with recent results derived from large galaxy surveys. (abridged)Comment: accepted for publication in Ap
Problematizing Choice: Responsible consumers and sceptical citizens
About the book: Governance, Consumers and Citizens is the first book to bring together a study of governance with consumption, examining the changing place of the consumer as citizen in recent trends in governance, the tensions between competing ideas and practices of consumerism and the active role consumers play in the construction and practice of governance.
Radically pushing forward the debate on consumers and governance, this collection outlines new conceptions and posits new policy agendas. Bringing together international experts from political science, history, geography, social policy and media studies, this study shows how governance and consumption are intertwined in crucial aspects of public policy and contemporary politics
Pillared two-dimensional metal-organic frameworks based on a lower-rim acid appended calix[4]arene
Solvothermal reactions of the lower-rim functionalized diacid calix[4]arene 25,27-bis(methoxycarboxylic acid)-26,28-dihydroxy-4-tert-butylcalix[4]arene (LH₂) with Zn(NO₃)₂•6H₂O and the dipyridyl ligands 4,4/-bipyridyl (4,4/-bipy), 1,2-di(4-pyridyl)ethylene (DPE) or 4,4/-azopyridyl (4,4/-azopy) afforded a series of 2-D structures of the formulae {[Zn(4,4/-bipy)(L)]•2¼DEF}n (1), {[Zn₂(L)(DPE)]•DEF}n (2) and {[Zn(OH₂)₂(L)(4,4/-azopy)]•DEF}n (3) (DEF = diethylformamide)
Ferumoxytol-enhanced MRI in patients with prior cardiac transplantation.
Objectives: Ultra-small superparamagnetic particles of iron oxide (USPIO)-enhanced MRI can detect cellular inflammation within tissues and may help non-invasively identify cardiac transplant rejection. Here, we aimed to determine the normal reference values for USPIO-enhanced MRI in patients with a prior cardiac transplant and examine whether USPIO-enhanced MRI could detect myocardial inflammation in patients with transplant rejection. Methods: Ten volunteers and 11 patients with cardiac transplant underwent T2, T2* and late gadolinium enhancement 1.5T MRI, with further T2* imaging at 24 hours after USPIO (ferumoxytol, 4 mg/kg) infusion, at baseline and 3 months. Results: Ten patients with clinically stable cardiac transplantation were retained for analysis. Myocardial T2 values were higher in patients with cardiac transplant versus healthy volunteers (53.8±5.2 vs 48.6±1.9 ms, respectively; p=0.003). There were no differences in the magnitude of USPIO-induced change in R2* in patients with transplantation (change in R2*, 26.6±7.3 vs 22.0±10.4 s-1 in healthy volunteers; p=0.28). After 3 months, patients with transplantation (n=5) had unaltered T2 values (52.7±2.8 vs 52.12±3.4 ms; p=0.80) and changes in R2* following USPIO (29.42±8.14 vs 25.8±7.8 s-1; p=0.43). Conclusion: Stable patients with cardiac transplantation have increased myocardial T2 values, consistent with resting myocardial oedema or fibrosis. In contrast, USPIO-enhanced MRI is normal and stable over time suggesting the absence of chronic macrophage-driven cellular inflammation. It remains to be determined whether USPIO-enhanced MRI may be able to identify acute cardiac transplant rejection. Trial registration number: NCT02319278349 (https://clinicaltrials.gov/ct2/show/NCT02319278) Registered 03.12.2014 EUDraCT 2013-002336-24
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