Neuropharmacological evaluation of Annona senegalensis leaves

The neuropharmacological activities of methanol leaf extract (ME) of Annona senegalensis Pers (Annonaceae) and its bioactive fractions (MF and F7) were studied in rodents using pentylenetetrazol (PTZ)-induced seizures, pentobarbitone-induced sleep, apomorphine-induced stereotypy, open field, elevated plus maze (EPM) and rotarod performance tests. The extract and fractions inhibited PTZ-induced seizures, prolonged pentobarbitone-induced sleep, reduced stereotypic behaviour induced by apomorphine, decreased the frequency of line crossing and centre square entries and increased rearing in the air in the open field. The frequency of grooming and rearing against the wall were decreased, whereas the duration of grooming increased. Also, the extract and fractions increased the duration of stay in the open arm when compared to the closed arm of the EPM, and reduced the average time spent on the rotarod. Acute toxicity test showed an oral LD50 of ME greater than 5 g/kg in mice. Phytochemical analysis showed that ME tested positive for carbohydrates, reducing sugar, resins, saponins,  tannins, steroids, terpenoids, alkaloids, flavonoids and glycosides; MF tested positive for saponins, steroids, terpenoids, alkaloids, carbohydrates, reducing sugar, flavonoids and glycosides; while F7 tested positive for flavonoids. These findings suggest that leaves of A. senegalensis possess anticonvulsant, central depressant and anxiolytic-like properties attributable to flavonoids.Keywords: Annona senegalensis, anticonvulsant, anxiolytic, sedative, stereotypy

Patients' views on improving sickle cell disease management in primary care: focus group discussion.

OBJECTIVES: To assess sickle cell disease (SCD) patient and carer perspectives on the primary care services related to SCD that they receive from their general practitioner (GP). DESIGN: A focus group discussion was used to elicit the views of patients about the quality of care they receive from their primary health-care providers and what they thought was the role of primary care in SCD management. The focus group discussion was video recorded. The recording was then examined by the project team and recurring themes were identified. A comparison was made with notes made by two scribes also present at the discussion. SETTING: Sickle Cell Society in Brent, UK. PARTICIPANTS: Ten participants with SCD or caring for someone with SCD from Northwest London, UK. MAIN OUTCOME MEASURES: Patients perceptions about the primary care services they received, and a list of key themes and suggestions. RESULTS: Patients and carers often bypassed GPs for acute problems but felt that GPs had an important role to play around repeat prescriptions and general health care. These service users believed SCD is often ignored and deemed unimportant by GPs. CONCLUSION: Participants wanted the health service to support primary health-care providers to improve their knowledge and understanding of SCD. Key themes and suggestions from this focus group have been used to help develop an educational intervention for general practice services that will be used to improve SCD management in primary care

Views of patients about sickle cell disease management in primary care: a questionnaire-based pilot study.

OBJECTIVES: To determine how patients with sickle cell disease (SCD) perceive the quality of care that they receive from their primary healthcare providers. DESIGN: A questionnaire-based pilot study was used to elicit the views of patients about the quality of care they have been receiving from their primary healthcare providers and what they thought was the role of primary care in SCD management. SETTING: Sickle Cell Society and Sickle Cell and Thalassaemia Centre, in the London Borough of Brent. PARTICIPANTS: One hundred questionnaires were distributed to potential participants with SCD between November 2010 and July 2011 of which 40 participants responded. MAIN OUTCOME MEASURES: Analysis of 40 patient questionnaires collected over a nine-month period. RESULTS: Most patients are generally not satisfied with the quality of care that they are receiving from their primary healthcare providers for SCD. Most do not make use of general practitioner (GP) services for management of their SCD. Collecting prescriptions was the reason most cited for visiting the GP. CONCLUSION: GPs could help improve the day-to-day management of patients with SCD. This could be facilitated by local quality improvement schemes in areas with high disease prevalence. The results of the survey have been used to help develop a GP education intervention and a local enhanced service to support primary healthcare clinicians with SCDs ongoing management

Kinetics and Equilibrium of Crude Oil Sorption from Aqueous Solution Using Borassus Aeothopum Coir

In this study, Borassus aethopum coir (BAC) which is an abundant, cheap, readily available and biodegradable agricultural waste was modified by acetylation using acetic anhydride and NBS catalyst at room temperature. This was to increase their hydrophobic properties and improve their sorption capacity in aqueous environment. The crude oil sorption kinetic data were fitted by intra-particle diffusion and pseudo-first order for raw BAC but the pseudo-second order kinetic model best fits crude oil sorption data for acetylated BAC.  The Langmuir and Freundlich models were used to elucidate the sorption behaviour of crude oil with raw and acetylated BAC. Freundlich isotherm gave the best fit for the equilibrium crude oil sorption data for acetylated BAC and raw BAC. Fourier transform infrared and scanning electron microscope analyses showed clear evidence of successful acetylation. The results presented and discussed in the study suggests that acetylated BAC (ABAC) is more suitable for crude oil sorption than raw BAC, therefore, possesses more potential for application and further development in treatment of oil spillage. Keywords: acetylated borassus aethopum coir, kinetics, crude oil sorption, sorbents, sorption capacity. DOI: 10.7176/CMR/11-2-02

Non-falciparum malaria infection and IgG seroprevalence among children under 15 years in Nigeria, 2018.

Plasmodium falciparum (Pf) is the dominant malaria parasite in Nigeria though P. vivax (Pv), P. ovale (Po), and P. malariae (Pm) are also endemic. Blood samples (n = 31,234) were collected from children aged 0-14 years during a 2018 nationwide HIV survey and assayed for Plasmodium antigenemia, Plasmodium DNA, and IgG against Plasmodium MSP1-19 antigens. Of all children, 6.6% were estimated to have Pm infection and 1.4% Po infection with no Pv infections detected. The highest household wealth quintile was strongly protective against infection with Pm (aOR: 0.11, 95% CI: 0.05-0.22) or Po (aOR= 0.01, 0.00-0.10). Overall Pm seroprevalence was 34.2% (95% CI: 33.3-35.2) with lower estimates for Po (12.1%, 11.6-12.5) and Pv (6.3%, 6.0-6.7). Pm seropositivity was detected throughout the country with several local government areas showing >50% seroprevalence. Serological and DNA indicators show widespread exposure of Nigerian children to Pm with lower rates to Po and Pv

Beyond ''women's traits'': exploring how gender, social difference and household characteristics influence trait preferences

Open Access Journal; Published online: 14 Dec 2021Demand-led breeding strategies are gaining importance in public sector breeding globally. While borrowing approaches from the private sector, public sector programs remain mainly focused on food security and social impact related outcomes. This necessitates information on specific user groups and their preferences to build targeted customer and product profiles for informed breeding decisions. A variety of studies have identified gendered trait preferences, but do not systematically analyze differences related to or interactions of gender with other social dimensions, household characteristics, and geographic factors. This study integrates 1000minds survey trait trade-off analysis with the Rural Household Multi-Indicator Survey to study cassava trait preferences in Nigeria related to a major food product, gari. Results build on earlier research demonstrating that women prioritize food product quality traits while men prioritize agronomic traits. We show that food product quality traits are more important for members from food insecure households and gender differences between men and women increase among the food insecure. Furthermore, respondents from poorer households prioritize traits similar to respondents in non-poor households but there are notable trait differences between men and women in poor households. Women in female headed household prioritized quality traits more than women living with a spouse. Important regional differences in trait preferences were also observed. In the South East region, where household use of cassava is important, and connection to larger markets is less developed, quality traits and in ground storability were prioritized more than in other states. These results reinforce the importance of recognizing social difference and the heterogeneity among men and women, and how individual and household characteristics interact to reveal trait preference variability. This information can inform trait prioritization and guide development of breeding products that have higher social impact, which may ultimately serve the more vulnerable and align with development goals

Pathways for the Valorization of Animal and Human Waste to Biofuels, Sustainable Materials, and Value-Added Chemicals

Human and animal waste, including waste products originating from human or animal digestive systems, such as urine, feces, and animal manure, have constituted a nuisance to the environment. Inappropriate disposal and poor sanitation of human and animal waste often cause negative impacts on human health through contamination of the terrestrial environment, soil, and water bodies. Therefore, it is necessary to convert these wastes into useful resources to mitigate their adverse environmental effect. The present study provides an overview and research progress of different thermochemical and biological conversion pathways for the transformation of human- and animal-derived waste into valuable resources. The physicochemical properties of human and animal waste are meticulously discussed, as well as nutrient recovery strategies. In addition, a bibliometric analysis is provided to identify the trends in research and knowledge gaps. The results reveal that the USA, China, and England are the dominant countries in the research areas related to resource recovery from human or animal waste. In addition, researchers from the University of Illinois, the University of California Davis, the Chinese Academy of Sciences, and Zhejiang University are front runners in research related to these areas. Future research could be extended to the development of technologies for on-site recovery of resources, exploring integrated resource recovery pathways, and exploring different safe waste processing methods

Pathways for the Valorization of Animal and Human Waste to Biofuels, Sustainable Materials and Value-Added Chemicals

Human and animal waste, including waste products originating from human or animal digestive systems such as urine, feces, and animal manure, have constituted a nuisance to the environment. Inappropriate disposal and poor sanitation of human and animal waste often cause negative impacts on human health through contamination of the terrestrial environment, soil, and water bodies. Therefore, it is necessary to convert these wastes into useful resources to mitigate their adverse environmental effect. The present study provides an overview and research progress of different thermochemical and biological conversion pathways for the transformation of human- and animal-derived waste into valuable resources. The physicochemical properties of human and animal waste are meticulously discussed as well as nutrient recovery strategies. In addition, a bibliometric analysis is provided to identify the trends in research and knowledge gaps. The results reveal that the U.S.A, China and England are the dominant countries in the research areas related to resource recovery from human or animal waste. In addition, researchers from the University of Illinois, the University of California Davis, the Chinese Academy of Science and Zhejiang University are front runners in research related to these areas. Future research should be centred on developing technologies for the on-site recovery of resources, exploring integrated resource recovery pathways, and exploring different safe waste processing methods

Effects of monosodium-L-glutamate administration on serum levels of reproductive hormones and cholesterol, epididymal sperm reserves and testicular histomorphology of male albino rats

This study investigated the effects of administration of monosodium L-glutamate (MSG) on serum gonadotrophin-releasing hormone (GnRH), luteinising hormone (LH), testosterone and total cholesterol (TC), cauda epididymal sperm reserves (CESR) and testicular histomorphology of adult male albino rats. Eighty-four rats, randomly assigned to 7 groups of 12 rats each, were used for the study. Varying low doses (0.25, 0.50 or 1.00 g/kg body weight) of MSG were administered orally or subcutaneously at 48-h intervals for six weeks. Serum GnRH, LH, testosterone and TC, and CESR were evaluated on days 14, 28 and 42 of MSG administration. Testicular histomorphology was evaluated on day 42. The results showed that the mean serum GnRH, LH and testosterone levels, and the CESR of all the treated groups were significantly (P < 0.05) lower than those of the untreated control on days 14, 28 and 42 of MSG administration. The mean serum TC levels of all the treated groups were also significantly (P < 0.05) lower than those of the control group on days 14 and 28. No lesions were observed on sections of the testes. It was concluded that MSG administration for 14, 28 and 42 days led to significantly lower serum levels of GnRH, LH, testosterone and TC, and significantly lower CESR

In Vivo CD8+ T-Cell Suppression of SIV Viremia Is Not Mediated by CTL Clearance of Productively Infected Cells

The CD8+ T-cell is a key mediator of antiviral immunity, potentially contributing to control of pathogenic lentiviral infection through both innate and adaptive mechanisms. We studied viral dynamics during antiretroviral treatment of simian immunodeficiency virus (SIV) infected rhesus macaques following CD8+ T-cell depletion to test the importance of adaptive cytotoxic effects in clearance of cells productively infected with SIV. As previously described, plasma viral load (VL) increased following CD8+ T-cell depletion and was proportional to the magnitude of CD8+ T-cell depletion in the GALT, confirming a direct relationship between CD8+ T-cell loss and viral replication. Surprisingly, first phase plasma virus decay following administration of antiretroviral drugs was not slower in CD8+ T-cell depleted animals compared with controls indicating that the short lifespan of the average productively infected cell is not a reflection of cytotoxic T-lymphocyte (CTL) killing. Our findings support a dominant role for non-cytotoxic effects of CD8+ T-cells on control of pathogenic lentiviral infection and suggest that cytotoxic effects, if present, are limited to early, pre-productive stages of the viral life cycle. These observations have important implications for future strategies to augment immune control of HIV