Using ICT to support public and private community memories: case studies and lessons learned

Information communication technologies (ICTs) enable the development of memories across a variety of communities. We identify a spectrum of deployment from private through to open public spaces. As we move along this spectrum key variables change including mechanisms of trust and accountability and the definition of ownership, authorship and readership. Some challenges however, remain constant such as designing for sustainability and the need to align research and community goals. Private spaces can be created to enhance existing interactions, develop bonding capital and build shared memory. Such spaces allow a defined membership the opportunity to explore new ideas away from the public gaze, using language which may not be intelligible to outsiders. ICTs may be used to bridge internal and external audiences, repurposing content for a wider public. The original content may require alternative presentation, organisation or navigation methods to support its effective use by an external audience. Increasingly, community memories are being developed using social software within the public sphere, however this raises issues of authority, reputation management, and conflict resolution. Unexpected innovation may occur, and issues of sustainability must be addressed. In our analysis we will draw on three ICT initiatives in which we have participated: Bletchley Park Guides’ Forum, Bletchley Park Text and Milton Keynes Open Guide

Knowledge support of simulation model reuse

This describes the knowledge support for engineering design based on virtual modelling and simulation. These are the results of the EC Clockwork project. A typical and important step in the development of a simulation model is the phase of reusing. Virtual modelling and simulation often use the components of previous models. The usual problem is that the only remaining part of the previous simulation models is the model itself. However, a large amount of knowledge and intermediate models have been used, developed and then lost. A special methodology and special tools have therefore been developed on support of storing, retrieving and reusing the knowledge from previous simulation models. The knowledge support includes informal knowledge, formal knowledge and intermediate engineering models. This paper describes the overall methodology and tools, using the example of developing a simulation model of Trijoint, a new machine tool

The melanoma-associated antigen 1 (MAGEA1) protein stimulates the E3 ubiquitin-ligase activity of TRIM31 within a TRIM31-MAGEA1-NSE4 complex

The MAGE (Melanoma-associated antigen) protein family members are structurally related to each other by a MAGEhomology domain comprised of 2 winged helix motifs WH/A and WH/B. This family specifically evolved in placental mammals although single homologs designated NSE3 (non-SMC element) exist in most eukaryotes. NSE3, together with its partner proteins NSE1 and NSE4 form a tight subcomplex of the structural maintenance of chromosomes SMC5–6 complex. Previously, we showed that interactions of the WH/B motif of the MAGE proteins with their NSE4/EID partners are evolutionarily conserved (including the MAGEA1-NSE4 interaction). In contrast, the interaction of the WH/A motif of NSE3 with NSE1 diverged in the MAGE paralogs. We hypothesized that the MAGE paralogs acquired new RING-finger containing partners through their evolution and form MAGE complexes reminiscent of NSE1-NSE3-NSE4 trimers. In this work, we employed the yeast 2-hybrid system to screen a human RING-finger protein library against several MAGE baits. We identified a number of potential MAGE-RING interactions and confirmed several of them (MDM4, PCGF6, RNF166, TRAF6, TRIM8, TRIM31, TRIM41) in co-immunoprecipitation experiments. Among these MAGE-RING pairs, we chose to examine MAGEA1-TRIM31 in detail and showed that both WH/A and WH/B motifs of MAGEA1 bind to the coiled-coil domain of TRIM31 and that MAGEA1 interaction stimulates TRIM31 ubiquitin-ligase activity. In addition, TRIM31 directly binds to NSE4, suggesting the existence of a TRIM31-MAGEA1-NSE4 complex reminiscent of the NSE1-NSE3-NSE4 trimer. These results suggest that MAGEA1 functions as a co-factor of TRIM31 ubiquitin-ligase and that the TRIM31-MAGEA1-NSE4 complex may have evolved from an ancestral NSE1-NSE3-NSE4 complex

Two-loop representations of low-energy pion form factors and pi-pi scattering phases in the presence of isospin breaking

Dispersive representations of the pi-pi scattering amplitudes and pion form factors, valid at two-loop accuracy in the low-energy expansion, are constructed in the presence of isospin-breaking effects induced by the difference between the charged and neutral pion masses. Analytical expressions for the corresponding phases of the scalar and vector pion form factors are computed. It is shown that each of these phases consists of the sum of a "universal" part and a form-factor dependent contribution. The first one is entirely determined in terms of the pi-pi scattering amplitudes alone, and reduces to the phase satisfying Watson's theorem in the isospin limit. The second one can be sizeable, although it vanishes in the same limit. The dependence of these isospin corrections with respect to the parameters of the subthreshold expansion of the pi-pi amplitude is studied, and an equivalent representation in terms of the S-wave scattering lengths is also briefly presented and discussed. In addition, partially analytical expressions for the two-loop form factors and pi-pi scattering amplitudes in the presence of isospin breaking are provided.Comment: 57 pages, 12 figure

Knowledge support for virtual modelling and simulation

The paper describes an approach to providing knowledge support for virtual modelling and simulation (VMS). The design methodology, based on multidisciplinary virtual modelling and subsequent simulation, is essential for contemporary engineering design, but specifically for the design of complex mechatronic machines. Additionally, current engineering design is multidisciplinary and therefore based on team work. The teams are often geographically distributed and would benefit from greater support for collaboration. Engineering design also draws heavily on previous experience and therefore it is essentially to build and maintain comprehensible and re-usable archives of previous cases. This is also true of simulation models

Blending coherence and control in the construction of interactive educational narratives from digital resources

Digital learning environments are generally composed of resources that cumulatively meet some specified educational objective, with each resource facilitating the acquisition of a subset of the concepts to be learned. In such contexts narrative has, for example, been used to support the understanding and navigation of a course or curriculum structure into which the resources have been pre-organised. Conversely, we focus on educational contexts where the unit of learning is concepts derived across the constituent resources. Such learning across resources often characterises more learner-directed, inquiry-based, exploratory, or informal learning activities. Here the task of the learner is to select, organise, and conceptualise collections of resources, a task that narrative can potentially support. Using narrative to aid learners in working across a set of resources introduces a perennial challenge of interactive narrative - how to appropriately facilitate both narrative coherence and user control. Narrative coherence can help the learner to reason and navigate across a set of resources. Learners also need sufficient user control over resource selection, organisation, and use so that they do not feel over-constrained in what they can explore. Murray made a thought-provoking comparison between reading an interactive narrative and viewing a sculpture. We use this as a starting point to derive desirable properties for interactive narrative that support learning across resources, namely inter- and intra-perspective coherence and control. As an example, we interpret two of our systems in terms of how they meet these properties and sketch some general guidelines for the design of interactive narrative systems that support learning across resources

Unraveling an FNR based regulatory circuit in Paracoccus denitrificans using a proteomics-based approach.

The switch from aerobic to anaerobic respiration in the bacterium Paracoccus denitrificans is orchestrated by the action of three FNR-type transcription regulators FnrP, NNR and NarR, which are sensors for oxygen, nitric oxide and nitrite, respectively. In this work, we analyzed the protein composition of four strains (wild type, FnrP-, NNR- and NarR-mutant strains) grown aerobically, semiaerobically and semiaerobically in the presence of nitrate to discover the global role of FNR-family transcription regulators using proteomics, with data validation at the transcript and genome levels. Expression profiles were acquired using two-dimensional gel electrophoresis for 737 protein spots, in which 640 proteins were identified using mass spectrometry. The annotated 2-D proteome map provided the most comprehensive coverage of P. denitrificans proteome available to-date and can be accessed on-line at http://www.mpiib-berlin.mpg.de/2D-PAGE/. Our results revealed several types of regulation under the conditions tested: (1) FnrP-controlled regulation of nitrous oxide reductase, UspA and OmpW as confirmed at protein, transcript and DNA level (position of FNR boxes). (2) Proteins regulated via additional regulators, including proteins involved in NNR and NarR regulons: nitrate reductase β-subunit, TonB-dependent receptors, nitrite reductase, a TenA-type transcription regulator, and an unknown protein with an alpha/beta hydrolase fold. (3) Proteins whose expression was affected mainly by the growth condition. This group contains SSU ribosomal protein S305 /