SESAR: The Past, Present, and Future of European Air Traffic Management Research

The Single European Sky ATM Research (SESAR) project is the technological pillar of the European Commission’s Single European Sky Initiative to modernize air traffic management (ATM). Here, we describe the process of establishing SESAR and the main parts of the project: the research and development (R&D) part, which is led by the SESAR Joint Undertaking; the deployment part, which is managed by the SESAR Deployment Manager; and the European ATM Master Plan, which collects and lays out both the R&D and deployment needs. The latest European ATM Master Plan was adopted just prior to the current pandemic. The huge loss in air traffic due to the pandemic, and the speed of the recovery of the aviation industry will require reprioritization, but the main elements that have been established—particularly those in support of the environment—remain valid

'There’s too many gay categories now': discursive constructions of gay masculinity

“Masculine capital” refers to the social power afforded by the display of traits and behaviors that are associated with orthodox, stereotypical masculinity. Men who are concerned with their masculine identity may utilize these traits and behaviors to increase their overall masculine capital, and to mitigate “failures” in other domains of masculinity. However, their success at accruing and trading masculine capital may be limited, because different traits and behaviors are not equal in the capital they convey, and their value may vary depending on the social context in which they are deployed. Research suggests that heterosexuality contributes more to masculine capital than other stereotypically masculine characteristics: The possibilities for gay men to accrue and trade masculine capital may therefore be particularly limited, especially in heteronormative contexts. Focus groups were undertaken with gay men, straight women and straight men living in a coastal city in the south of England to explore discursive constructions of gay masculinity, and to examine gay men’s possibilities for accruing and trading masculine capital. Discourse analysis identified constructions of gay masculinity in reference to hegemonic masculinity, where gay men may acquire masculine capital in similar ways to straight men. However, the meaning and value of this capital may also vary, because certain characteristics and behaviors may have different value for and between gay men than they do for straight men, and in heteronormative contexts. The analysis also identified discourses of gay masculinity where it was not constructed as a singular entity, but rather as complex, multiple and diverse

"I don't want to be seen as a screaming queen": an interpretative phenomenological analysis of gay men's masculine identities

It has been argued that gay men who live in Western societies must negotiate masculine identities against a cultural backdrop where the most desirable and locally hegemonic masculinity is heterosexual. However, contemporary masculinity theories conceptualize masculinities as increasingly inclusive of gay men. The purpose of this study was to use a discourse-dynamic approach to studying masculine subjectivity to identify how gay men in England and Wales negotiated masculinity discourses to construct their masculine identities. One-to-one, semi-structured interviews were undertaken with six younger gay men aged 20 to 24, and 11 older gay men aged 30 to 42. Participants were asked to describe their subjective experiences of masculinity. The results of an Interpretative Phenomenological Analysis indicated that discourses of hegemonic and alternative masculinities had implications for lived experiences of masculinity. Older participants in particular emphasized their attributes they associated with masculine dominance, including anti-effeminacy attitudes. The majority of younger participants did not feel masculine. Irrespective of age, many participants resisted hegemonic masculinity by highlighting the value of “gayness” at times. The findings suggested that hegemonic masculinity was the most readily available discourse for conceptualizing masculinity, but that lived experiences of masculinity were not necessarily located within this discourse

Sexuality, risk, and organisational misbehaviour in a secure mental healthcare facility in England

Sexuality in secure mental healthcare has been overlooked in both clinical praxis and academic research. In the UK, there exist no formal policies to inform staff approaches to managing inpatient sexuality. The limited research that has been undertaken in this field has found that often, prohibitive approaches are favoured, which may affect how inpatients conceptualise and experience their sexuality in the long-term. The aim of this study was to identify discursive constructions of inpatient sexuality, as articulated within semi-structured group interviews with inpatients and ward staff from a secure mental healthcare facility in England. The analysis identified constructions of inpatient sexuality within two overarching and conflicting discourses: one of the normalcy and legitimacy of sexual expression in human experience; and the other of risk, wherein sexuality needed to be regulated and obstructed. Inpatients’ expressions of sexuality could often only be conceptualised in terms of “organisational misbehaviour”, acts that violated the implicit norms and codes of the institution. It is suggested that recoding inpatient sexuality as misbehaviour could have implications for inpatients’ long-term recovery

Choreographies of sexual safety: forensic mental health and the limits of recovery

Medium secure forensic psychiatric units are unique environments within the broader “post asylum” landscape of mental health services. Length of stay is much greater and restrictions on behavior, including sexual behavior, are legally and institutionally legitimated, due to concerns regarding risk. As a result, sexuality is rarely explored with service users and no official policies on sexual conduct and sexual safety have yet been developed, despite sexuality being linked to positive recovery outcomes

Comprehensive cell surface proteomics defines markers of classical, intermediate and non-classical monocytes

Abstract: Monocytes are a critical component of the cellular innate immune system, and can be subdivided into classical, intermediate and non-classical subsets on the basis of surface CD14 and CD16 expression. Classical monocytes play the canonical role of phagocytosis, and account for the majority of circulating cells. Intermediate and non-classical cells are known to exhibit varying levels of phagocytosis and cytokine secretion, and are differentially expanded in certain pathological states. Characterisation of cell surface proteins expressed by each subset is informative not only to improve understanding of phenotype, but may also provide biological insights into function. Here we use highly multiplexed Tandem-Mass-Tag (TMT)-based mass spectrometry with selective cell surface biotinylation to characterise the classical monocyte surface proteome, then interrogate the phenotypic differences between each monocyte subset to identify novel protein markers

Quantitative comparative analysis of human erythrocyte surface proteins between individuals from two genetically distinct populations

Abstract: Red blood cells (RBCs) play a critical role in oxygen transport, and are the focus of important diseases including malaria and the haemoglobinopathies. Proteins at the RBC surface can determine susceptibility to disease, however previous studies classifying the RBC proteome have not used specific strategies directed at enriching cell surface proteins. Furthermore, there has been no systematic analysis of variation in abundance of RBC surface proteins between genetically disparate human populations. These questions are important to inform not only basic RBC biology but additionally to identify novel candidate receptors for malarial parasites. Here, we use ‘plasma membrane profiling’ and tandem mass tag-based mass spectrometry to enrich and quantify primary RBC cell surface proteins from two sets of nine donors from the UK or Senegal. We define a RBC surface proteome and identify potential Plasmodium receptors based on either diminished protein abundance, or increased variation in RBCs from West African individuals

Comprehensive cell surface proteomics defines markers of classical, intermediate and non-classical monocytes

Abstract: Monocytes are a critical component of the cellular innate immune system, and can be subdivided into classical, intermediate and non-classical subsets on the basis of surface CD14 and CD16 expression. Classical monocytes play the canonical role of phagocytosis, and account for the majority of circulating cells. Intermediate and non-classical cells are known to exhibit varying levels of phagocytosis and cytokine secretion, and are differentially expanded in certain pathological states. Characterisation of cell surface proteins expressed by each subset is informative not only to improve understanding of phenotype, but may also provide biological insights into function. Here we use highly multiplexed Tandem-Mass-Tag (TMT)-based mass spectrometry with selective cell surface biotinylation to characterise the classical monocyte surface proteome, then interrogate the phenotypic differences between each monocyte subset to identify novel protein markers

Human cytomegalovirus interactome analysis identifies degradation hubs, domain associations and viral protein functions

Human cytomegalovirus (HCMV) extensively modulates host cells, downregulating >900 human proteins during viral replication and degrading ≥133 proteins shortly after infection. The mechanism of degradation of most host proteins remains unresolved, and the functions of many viral proteins are incompletely characterised. We performed a mass spectrometry-based interactome analysis of 169 tagged, stably-expressed canonical strain Merlin HCMV proteins, and two non-canonical HCMV proteins, in infected cells. This identified a network of >3,400 virus-host and >150 virus-virus protein interactions, providing insights into functions for multiple viral genes. Domain analysis predicted binding of the viral UL25 protein to SH3 domains of NCK Adaptor Protein-1. Viral interacting proteins were identified for 31/133 degraded host targets. Finally, the uncharacterised, non-canonical ORFL147C protein was found to interact with elements of the mRNA splicing machinery, and a mutational study suggested its importance in viral replication. The interactome data will be important for future studies of herpesvirus infection

Conducting sexualities research: An outline of emergent issues and case studies from ten wellcome-funded projects [version 1; peer review: 3 approved]

© 2019 Kneale D et al. This letter seeks to synthesise methodological challenges encountered in a cohort of Wellcome Trust-funded research projects focusing on sexualities and health. The ten Wellcome Trust projects span a diversity of gender and sexual orientations and identities, settings; institutional and non-institutional contexts, lifecourse stages, and explore a range of health-related interventions. As researchers, we originate from a breadth of disciplinary traditions, use a variety of research methods and data sources. Despite this breadth, four common themes are found across the projects: (i) inclusivity representations and representativeness, (ii) lumping together of diverse groups, (iii) institutions and closed settings (iv) ethical and governance barriers