Morphological, cultural and pathogenic variability in Alternaria brassicae, the causing agent of black spot of rapeseed and mustard

The study on pathogenic diversity of twenty isolates of Alternaria brassicae collected from different locations of Uttarakhand and Central Uttar Pradesh infecting Brassica species (Brassica rapa, Brassica juncea and Eruca sativa) revealed that there was a distinct difference among isolates in terms of mycelial growth, spore length, width, spore beak length and width. The average spore length varied from 21.23?m to 38.13?m with minimum of isolate AUA-19, AUA-43 i.e 21.23?m and maximum of AUA-47 i.e. 38.13?m . The isolates tested on Brassica juncea var.Varuna in green house conditions revealed that all the twenty isolates behaved differently. Among all the isolates, Brassica juncea isolates i.e. AUA-25, AUA-39, AUA-41, AUA-47, AUA-19, AUA-24, AUA-22, AUA-21, AUA-31, AUA-43 and AUA-45 from Uttarakhand, and AUP-29 from Central Uttar Pradesh can be grouped into highly pathogenic with range of Alternaria spot size i.e. 5.03-8.30mm in diameter, while isolate of Eruca sativa i.e. AUA-38 was found least pathogenic with 1.63mm in dia. and eight isolates AUA-18, AUA-20, AUA-23, AUP-28, AUA-32, AUA-33 and AUA-36 were found moderately pathogenic. This study will be useful in developing integrated management strategies of Alternaria leaf spot and breeding programs of oilseed crops (Brassica sp.)

Multi-wavelength polarimetric study towards the open cluster NGC 1893

We present multi-wavelength linear polarimetric observations for 44 stars of the NGC 1893 young open cluster region along with V-band polarimetric observations of stars of other four open clusters located between l ~160 to ~175 degree. We found evidence for the presence of two dust layers located at a distance of ~170 pc and ~360 pc. The dust layers produce a polarization Pv ~2.2%. It is evident from the clusters studied in the present work that, in the Galactic longitude range l ~160 to 175 degree and within the Galactic plane (|b| < 2 degree), the polarization angles remain almost constant, with a mean ~163 degree and a dispersion of 6 degree. The small dispersion in polarization angle could be due to the presence of uniform dust layer beyond 1 kpc. Present observations reveal that in case of NGC 1893, the foreground two dust layers, in addition to the intracluster medium, seems to be responsible for the polarization effects. It is also found that towards the direction of NGC 1893, the dust layer that exists between 2-3 kpc has a negligible contribution towards the total observed polarization. The weighted mean for percentage of polarization (Pmax) and the wavelength at maximum polarization ({\lambda}max) are found to be 2.59 \pm 0.02% and 0.55 \pm 0.01 \mum respectively. The estimated mean value of {\lambda}max indicates that the average size of the dust grains within the cluster is similar to that in the general interstellar medium. The spatial variation of the polarization is found to decrease towards the outer region of the cluster. In the present work, we support the notion, as already has been shown in previous studies, that polarimetry, in combination with (U-B)/(B-V) colour-colour diagram, is a useful tool for identifying non-members in a cluster.Comment: 36 pages, 12 figures, 10 tables, accepted for the publication in MNRA

An integrated portable system for single chip simultaneous measurement of multiple disease associated metabolites

Metabolites, the small molecules that underpin life, can act as indicators of the physiological state of the body when their abundance varies, offering routes to diagnosis of many diseases. The ability to assay for multiple metabolites simultaneously will underpin a new generation of precision diagnostic tools. Here, we report the development of a handheld device based on complementary metal oxide semiconductor (CMOS) technology with multiple isolated micro-well reaction zones and integrated optical sensing allowing simultaneous enzyme-based assays of multiple metabolites (choline, xanthine, sarcosine and cholesterol) associated with multiple diseases. These metabolites were measured in clinically relevant concentration range with minimum concentrations measured: 25 μM for choline, 100 μM for xanthine, 1.25 μM for sarcosine and 50 μM for cholesterol. Linking the device to an Android-based user interface allows for quantification of metabolites in serum and urine within 2 min of applying samples to the device. The quantitative performance of the device was validated by comparison to accredited tests for cholesterol and glucose

Broad-band Optical Polarimetric Studies toward the Galactic young star cluster Be 59

We present multiwavelength optical linear polarimetric observations of 69 stars toward the young open cluster Be 59. The observations reveal the presence of three dust layers located at the distances of \sim300, \sim500 and \sim700 pc. The dust layers produce a total polarization Pv \sim 5.5 per cent. The mean values of polarization and polarization angles due to the dust layers are found to increase systematically with distance. We show that polarimetry in combination with the (U - B) - (B - V) colour-colour diagram yields a better identification of cluster members. The polarization measurements suggest that the polarization due the intra-cluster medium is \sim 2.2 per cent. An anomalous reddening law exists for the cluster region, indicating a relatively larger grain size than that in the diffuse ISM. The spatial variation of the polarization and E(B - V) is found to increase with radial distance from the cluster center, whereas the {\theta}v and {\lambda}max are found to decrease with increasing radial distance from the cluster center. About 40 per cent of cluster members show the signatures of either intrinsic polarization or rotation in their polarization angles. There is an indication that the star light of the cluster members might have been depolarized because of non-uniform alignment of dust grains in the foreground dust layers and in the intra-cluster medium.Comment: 22 pages, 14 figures and 7 tables, Accepted in MNRA

Loss-of-Function in SMAD4 Might Not Be Critical for Human Natural Killer Cell Responsiveness to TGF-β

We characterized the NK cell phenotype and function in three family members with Hereditary Hemorrhagic Telangiectasia (HHT) due to heterozygous SMAD4 mutations. Loss-of-function mutation in this gene did not induce developmental effects to alter CD56bright or CD56dim NK cell subset proportions in peripheral blood; and did not result in major differences in either their IL-15-induced proliferation, or their cytokine secretion response to TGF-β1. These data suggest that SMAD4 plays a redundant role in downstream TGF-β signaling in NK cells

The effects of pulmonary rehabilitation in patients with non-cystic fibrosis bronchiectasis: protocol for a randomised controlled trial

BACKGROUND: Non-cystic fibrosis bronchiectasis is characterised by sputum production, exercise limitation and recurrent infections. Although pulmonary rehabilitation is advocated for this patient group, its effects are unclear. The aims of this study are to determine the short and long term effects of pulmonary rehabilitation on exercise capacity, cough, quality of life and the incidence of acute pulmonary exacerbations. METHODS/DESIGN: This randomised controlled trial aims to recruit 64 patients with bronchiectasis from three tertiary institutions. Participants will be randomly allocated to the intervention group (supervised, twice weekly exercise training with regular review of airway clearance therapy) or a control group (twice weekly telephone support). Measurements will be taken at baseline, immediately following the intervention and at six and 12 months following the intervention period by a blinded assessor. Exercise capacity will be measured using the incremental shuttle walk test and the six-minute walk test. Quality of life and health status will be measured using the Chronic Respiratory Questionnaire, Leicester Cough Questionnaire, Assessment of Quality of Life Questionnaire and the Hospital Anxiety and Depression Scale. The rate of hospitalisation will be captured as well as the incidence of acute pulmonary exacerbations using a daily symptom diary. DISCUSSION: Results from this study will help to determine the efficacy of supervised twice-weekly pulmonary rehabilitation upon exercise capacity and quality of life in patients with bronchiectasis and will contribute to clinical practice guidelines for physiotherapists in the management of this population. TRIAL REGISTRATION: This study protocol is registered with ClinicalTrials.gov (NCT00885521)