Distribución temporal y espacial de Paranortes cordicollis y Pachymorphus striatulus (Coleoptera, Carabide) en dos ambientes edáficos de la Laguna Nahuel Rucá, Buenos Aires, Argentina

Fil: Porrini, Darío P.. CONICETFil: Castro, Adela V.. CONICETFil: Cicchino, Armando Conrado. División Entomología. Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata; Argentin

Riqueza específica y estructura de dominancia del ensamble de carábidos edáficos (Coleoptera, Carabidae) del saucedal (Salix humboldtiana) de Laguna de los Padres (Buenos Aires, Argentina) durante el período invernal

Fil: Porrini, Darío P.. CONICETFil: Castro, Adela V.. CONICETFil: Cicchino, Armando Conrado. División Entomología. Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata; Argentin

Dominancia estacional de las Carabidae edáficas (Insecta, Coleoptera) en dos ambientes de la Reserva Integral Laguna de los Padres, Partido de General Pueyrredón, provincia de Buenos Aires

Fil: Cicchino, Armando Conrado. División Entomología. Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata; ArgentinaFil: Castro, Adela V.. CONICETFil: Porrini, Darío P.. CONICE

Distribución temporal y espacial de Loxandrus simplex y Loxandrus planicollis (Coleoptera, Carabide) en la Laguna Nahuel Rucá

Fil: Castro, Adela V.. CONICETFil: Porrini, Darío P.. CONICETFil: Cicchino, Armando Conrado. División Entomología. Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata; Argentin

Rasgos biológicos y fenología estacional de Bradycellus viduus (Dejean, 1829) (Insecta, Coleoptera, Harpalini) en el sudeste de la provincia de Buenos Aires, Argentina

Fil: Cicchino, Armando Conrado. División Entomología. Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata; ArgentinaFil: Castro, Adela V.. CONICETFil: Porrini, Darío P.. CONICE

Riqueza específica de las Carabidae (Insecta, Coleoptera) de los suelos de la Reserva Integral Laguna de los Padres, Partido de General Pueyrredón, provincia de Buenos Aires : Resultados de diez años de muestreos (1999-2009)

Fil: Cicchino, Armando Conrado. División Entomología. Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata; ArgentinaFil: Porrini, Darío P.. CONICETFil: Castro, Adela V.. CONICE

Diversidad de escarabajos de la familia Carabidae en un ambiente peridomiciliario del talar de Laguna Nahuel Rucá, Mar Chiquita

Fil: Castro, Adela V.. Laboratorio de Artrópodos. Departamento de Biología. Facultad de Ciencias Exactas y Naturales. Universidad Nacional de Mar del Plata; ArgentinaFil: Porrini, Darío P.. Laboratorio de Artrópodos. Departamento de Biología. Facultad de Ciencias Exactas y Naturales. Universidad Nacional de Mar del Plata; ArgentinaFil: Cicchino, Armando Conrado. División Entomología. Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata; Argentin

Anthocyanins and metabolites resolve TNF-α-mediated production of E-selectin and adhesion of monocytes to endothelial cells

This study investigated the capacity of an anthocyanin-rich fraction (ACN-RF) from blueberry, single anthocyanins (cyanidin, delphinidin and malvidin-3-glucoside; Cy, Dp and Mv-3-glc) and related metabolites (protocatechuic, gallic and syringic acid; PrA, GA and SA) to resolve an inflammation-driven adhesion of monocytes (THP-1) on endothelial cell (HUVECs) and secretion of cell adhesion molecules E-selectin and vascular cell adhesion molecule 1 (VCAM-1). The adhesion of THP-1 to HUVECs was induced by tumour necrosis factor \u3b1 (TNF-\u3b1, 100\u202fng\u202fmL-1). Subsequently, ACN-RF, single ACNs and metabolites (from 0.01 to 10\u202f\u3bcg\u202fmL-1) were incubated for 24\u202fh. The adhesion was measured in a fluorescence spectrophotometer. E-selectin and VCAM-1 were quantified by ELISA. No toxicological effects were observed for the compounds and the doses tested. ACN-RF and Mv-3-glc reducedTHP-1 adhesion at all the concentrations with the maximum effect at 10 \u3bcg/ml (-60.2% for ACNs and-33.9% for Mv-3-glc). Cy-3-glc decreased the adhesion by about 41.8% at 10\u202f\u3bcg\u202fmL-1, while PrA and GA reduced the adhesion of THP-1 to HUVECs both at 1 and at 10\u202f\u3bcg\u202fmL-1 (-29.5% and -44.3% for PrA, respectively, and -18.0%and -59.3% for GA, respectively). At the same concentrations a significant reduction of E-selectin, but notVCAM-1 levels, was documented. No effect was observed following Dp-3-glc and SA supplementation. Overall, ACNs and metabolites seem to resolve, in a dose-dependent manner, the inflammation-driven adhesion of THP-1 to HUVECs by decreasing E-selectin concentrations. Interestingly, Mv-3-glc was active at physiologically relevant concentrations

Different effects of anthocyanins and phenolic acids from wild blueberry (Vaccinium angustifolium) on monocytes adhesion to endothelial cells in a TNF-alpha stimulated proinflammatory environment

SCOPE: Monocyte adhesion to the vascular endothelium is a crucial step in the early stages of atherogenesis. This study aims to investigate the capacity of an anthocyanin (ACN) and phenolic acid (PA)-rich fraction (RF) of a wild blueberry, single ACNs (cyanidin, malvidin, delphinidin) and related metabolites (protocatechuic, syringic and gallic acid) to counteract monocytes (THP-1) adhesion to endothelial cells (HUVECs) in a tumor necrosis \u3b1 (TNF-\u3b1) mediated pro-inflammatory environment. METHODS AND RESULTS: HUVECs were incubated with different concentrations (from 0.01 to 10 \u3bcg mL-1 ) of the compounds for 24 h. Labelled monocytic THP-1 cells were added to HUVECs and their adhesion was induced by TNF-\u3b1 (100 ng mL-1 ). ACN-RF reduced THP-1 adhesion to HUVECs with a maximum effect at 10 \u3bcg mL-1 (-33%). PA-RF counteracted THP-1 adhesion at 0.01, 0.1 and 1 \u3bcg mL-1 (-45%, -48.7% and -27.6%, respectively), but not at maximum concentration. Supplementation with gallic acid reduced THP-1 adhesion to HUVECs with a maximum effect at 1 \u3bcg mL-1 (-29.9%), while malvidin-3-glucoside and syringic acid increased the adhesion. No effect was observed for the other compounds. CONCLUSION: These results suggest that ACNs/PA-RF may prevent atherogenesis while the effects of the single ACNs and metabolites are controversial and merit further exploration

Role of polyphenols and polyphenol-rich foods in the modulation of PON1 activity and expression

Paraoxonase 1 (PON1) is a high-density lipoprotein (HDL)-associated enzyme involved in the protection of low-density lipoprotein and HDLs against lipid peroxidation. Several studies documented the capacity of polyphenols to stimulate PON1 transcription activation. The objective of the present review is to provide the main evidence about the role and the potential mechanism of action of polyphenols and polyphenol-rich foods in the modulation of PON1 gene expression and activity. A total of 76 in vitro and in vivo studies were included in the review. Overall, while evidence obtained in vitro is limited to quercetin and resveratrol, those deriving from animal models seem more convincing for a wide range of polyphenols but only at pharmacological doses. Evidence from human studies is promising but deserves more substantiation about the role of polyphenol-rich foods in the regulation of PON1 activity and expression. Research focused on the understanding of the structure\u2013activity relationship of polyphenols with PON1 and on the mechanisms at the base of PON1 modulation is warranted. Well-designed human intervention studies are encouraged to corroborate the findings of polyphenols also at physiological doses