111 research outputs found
Degradation of higher education in Kazakhstan as an example of post-transitional crisis
It is shown, that objectives of degradation of Higher Education in most part of post-soviet countries are connected with the specific factors appeared at the economical situation appeared at the end of transitional period (transition from planned economy to market one). Decreasing of quality of higher education in such countries as Kazakhstan may be interpreted in frameworks of Baudrillardβs concept of evolution of connection between "The Sign" and "The Reality"; such interpretation directly shows that overcoming of negative trends in post-soviet countries education cannot be realized by traditional administrative instruments. The problem may be solved by taking into account concepts of institutional economy
Cytokines and local chronic inflammation in the formation of infertility in fertile age women
The purpose of the study was to examine the characteristics of the content of cytokines in the peritoneal fluid in women of reproductive age with infertility against the background of chronic inflammatory diseases of the pelvic organs in the stage of clinical remission.Material and methods. A study was conducted of 50 patients aged 23-36 years with a verified diagnosis of infertility of tubal peritoneal genesis against a background of chronic inflammatory diseases of the reproductive sphere in the stage of clinical remission. As a control group, 15 practically healthy women aged 25-37 years were examined. Concentration of cytokines TNF-a, IL-4, IL-6, IL-10, IFN-y, MCP-1 in the peritoneal fluid was measured by enzyme-linked immunosorbent assay.Results and discussion. In the main group of patients with tubal-peritoneal infertility, the activity of local inflammatory reactions is tested in the period of clinical remission of a chronic inflammatory process. This is confirmed by a significantly higher level of TNF-a, IL-4, IL-6, IFN-y, MCP-1 in the peritoneal fluid compared to the control group. The studied biologically active substances play a significant role in the development of the immune response, as well as fibroplastic processes. The results can be regarded as the latent development of the immune response in reactions to constant antigenic stimulation from the focus of chronic inflammation, which is a significant factor in the development of adhesions in the formation of infertility, and requires the inclusion of immunotropic therapy methods in treatment
AB0-incompatibility of mother and fetus: the role of anti-glycan alloantibodies in the hemolytic disease of newborns
The mother and fetus incompatibility due to Rh-factor, blood group or other blood factors can lead to hemolytic disease of the fetus and newborn (HDN). HDN is a clinical disease condition of the fetus and newborn as a result of hemolysis, when maternal IgG alloantibodies cross the placenta and destroy the red blood cells of the fetus and newborn. The child disease begins in utero and can dramatically increase immediately after birth. As a result, hyperbilirubinemia and anemia develop, that can lead to abortions, serious complications, or death of the neonates in the absence of proper therapy. The range of HDN has changed significantly now compared to previous decades. Half a century ago, HDN was considered an almost complete synonym of RhD-alloimmunization, and this was a frequent problem for newborns. By now due to the high effective of Rh-conflict prevention, immunological AB0-conflicts have become the most common cause of HDN. The review aimes to one of the main causes of jaundice and anemia in neonates at present, i.e. HDN due to immunological AB0-conflict of mother and newborn (AB0-HDN). The main participants of the AΠ0- incompatibility mother and child are considered, namely A- and B-glycans, as well as the corresponding anti-glycan alloantibodies. Close attention is paid to the structure features of glycan alloantigens on the red blood cells of the fetus and adult. The possible correlation of the frequency and severity of HDN with the blood group of mother and child, as well as with the titer of maternal alloantibodies, has been considered. The influence of immunoglobulin G subclasses on the AB0-HDN development has been evaluated. In most cases, AB0-HDN appear when the mother has the blood group 0, and the fetus has the group A (subgroup A1) or the group B. Other rare incidences of AB0-incompatibility with severe course are occurred. As a whole the etiology of AB0-HDN is complex and the HDN severity is influenced by many factors. The authors have analyzed statistical data, as well as the prevalence of AB0-incompatibility and AB0-HDN in various regions of the world. Current approaches to the diagnosis of AB0-HDN are discussed in addition. By now the problems of AB0- HDN occurrence and developing of ways to overcome this disease remain relevant
Features of the immune response in children with acute respiratory infections caused by DNA and RNA viruses
The issues of immunopathogenesis of acute respiratory viral infections (ARVI) remain relevant, despite a long history of study. The aim of the study was to investigate the characteristics of the content of some cytokines in the blood serum of children with ARVI caused by DNA and RNA-containing viruses.Β Material and methods.Β We examined 92 children with ARVI at the age from 1 to 15 years, hospitalized in the hospital of the Childrenβs Clinical Hospital No. 6 (Novosibirsk). In order to determine the etiological factor, a study was carried out using the RT-PCR method (test systems AmpliSensORVI-screen-FL, βInterLabServiceβ, Russia) of mucus from the nose and throat for the presence of genetic material of viruses that cause ARVI. Determination of the content of IL-8, IL-17, IFN-Ξ³ in the blood serum in children of all groups was carried out by the method of solid-phase ELISA using commercial test systems of LLC βCytokinβ (Russia).Β Results and discussion.Β 4 groups were formed: group I (nΒ = 20) children with ARVI caused by DNA-containing viruses (group B, C, E adenoviruses, bocaviruses); group II (nΒ = 53) children with ARVI caused by RNA viruses (RS virus, metapneumovirus, parainfluenza viruses 1, 2, 3, 4, rhinovirus and coronoviruses); group III (nΒ = 12) children with ARVI caused by mixed infection; group IV (nΒ = 7) β the genetic material of the pathogen has not been isolated. Concentration of IL-8, IL-17, IFN-Ξ³ was significantly increased in all groups compared to the normative values. Higher values of IFN-Ξ³ and IL-8 were revealed in patients with DNA viruses compared with the group with RNA-containing viruses, although the excess of IFN-Ξ³ was not statistically significant. Differences in the content of IFN-Ξ³, IL-8 and IL-17 were noted for various etiological viral agents, but they were not significant.Β Conclusions.Β The results obtained can serve as confirmation that the functional characteristics of the response of the immune system in children with ARVI are determined not so much by the etiological factor as by its individual state. With ARVI in children, the presence of genetic material from more than one virus, apparently, is not a significant potentiating factor in the activation of the immune response. At the same time, a negative PCR result with a detailed clinical picture of ARVI does not exclude the viral genesis of the disease
ΠΠΠ’ΠΠ ΠΠΠ Π€ΠΠΠ’ΠΠ Π‘ΠΠΠ Π―ΠΠΠΠΠ― ΠΠΠ Π£Π¨ΠΠΠΠ ΠΠΠΠΠ ΠΠΠ¬ΠΠΠΠ Π Π£ΠΠΠΠΠΠΠΠΠΠ ΠΠΠΠΠΠ‘Π’ΠΠΠ ΠΠ Π ΠΠΠΠΠΠ§ΠΠ‘Π’ΠΠΠΠΠ«Π₯ ΠΠΠΠΠΠΠ ΠΠΠΠΠΠΠΠ―Π₯ ΠΠΠΠ’ΠΠΠΠΠΠ¬ΠΠ«Π₯ Π’ΠΠΠΠΠ
The impact of changes in orientation of the metabolism of carbohydrates and minerals in the cell malignancy has been demonstrated in several studies. The aim of this study was to analyze the molecular mechanisms and relationship of carbohydrate and mineral homeostasis with the processes of carcinogenesis. Parameters of carbohydrate and mineral metabolism of blood were defined in 73 patients with malignant tumors of epithelial tissues and 31 healthy subjects. In the presence of malignant tumors of epithelial tissues there was a statistically significant increase in the levels of glucose and glycated hemoglobin in the early stages of the disease and the absence of them at stage IV of the disease. There were no statistically significant differences in the levels of C-peptide and immunoreactive insulin in blood samples of cancer patients, although they tended to increase compared with the control group. Analysis of the composition of macroelements at the early stages of carcinogenesis revealed a statistically significant reduction of sodium level in plasma which wasnβt observed at the terminal stage of the disease. The concentrations of potassium and chlorine tend to increase in cancer patients, but the differences between these parameters were not statistically significant. Concentrations of calcium and magnesium significantly increased in the presence of malignant tumors. Analysis of microelements in the blood plasma showed a decrease in the concentration of cuprum and lithium (in 2.5-5 times) and the growth of strontium concentrations. Lithium has multiple effects on the life of cells, affecting a number of elements of messengers, as well as being the link between the carbohydrate metabolism and cell malignancy. Disorders of mineral homeostasis are important element in the disintegration of the metabolic processes in carcinogenesisΠΠ»ΠΈΡΠ½ΠΈΠ΅ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½ΠΎΡΡΠΈ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΠ·ΠΌΠ° ΡΠ³Π»Π΅Π²ΠΎΠ΄ΠΎΠ² ΠΈ ΠΌΠΈΠ½Π΅ΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΎΠ±ΠΌΠ΅Π½Π° Π½Π° ΠΌΠ°Π»ΠΈΠ³Π½ΠΈΠ·Π°ΡΠΈΡ ΠΊΠ»Π΅ΡΠΎΠΊ Π±ΡΠ»ΠΎ Π½Π°Π³Π»ΡΠ΄Π½ΠΎ ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ Π² ΡΡΠ΄Π΅ ΡΠ°Π±ΠΎΡ. Π¦Π΅Π»ΡΡ Π΄Π°Π½Π½ΠΎΠ³ΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΡΡΠ°Π» Π°Π½Π°Π»ΠΈΠ· ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΡ
ΠΌΠ΅Ρ
Π°Π½ΠΈΠ·ΠΌΠΎΠ² Π²Π·Π°ΠΈΠΌΠΎΡΠ²ΡΠ·ΠΈ ΡΠ³Π»Π΅Π²ΠΎΠ΄Π½ΠΎΠ³ΠΎ ΠΈ ΠΌΠΈΠ½Π΅ΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ Π³ΠΎΠΌΠ΅ΠΎΡΡΠ°Π·Π° Ρ ΠΏΡΠΎΡΠ΅ΡΡΠ°ΠΌΠΈ ΠΊΠ°Π½ΡΠ΅ΡΠΎΠ³Π΅Π½Π΅Π·Π°. ΠΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΡ ΡΠ³Π»Π΅Π²ΠΎΠ΄Π½ΠΎΠ³ΠΎ ΠΈ ΠΌΠΈΠ½Π΅ΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΎΠ±ΠΌΠ΅Π½ΠΎΠ² ΠΊΡΠΎΠ²ΠΈ Ρ 73 Π±ΠΎΠ»ΡΠ½ΡΡ
Π·Π»ΠΎΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΠΌΠΈ Π½ΠΎΠ²ΠΎΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡΠΌΠΈ ΡΠΏΠΈΡΠ΅Π»ΠΈΠ°Π»ΡΠ½ΡΡ
ΡΠΊΠ°Π½Π΅ΠΉ ΠΈ 31 ΠΏΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΈ Π·Π΄ΠΎΡΠΎΠ²ΡΡ
Π»ΠΈΡ. ΠΡΠΈ Π·Π»ΠΎΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΡ
Π½ΠΎΠ²ΠΎΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡΡ
ΡΠΏΠΈΡΠ΅Π»ΠΈΠ°Π»ΡΠ½ΡΡ
ΡΠΊΠ°Π½Π΅ΠΉ Π²ΡΡΠ²Π»Π΅Π½ΠΎ ΡΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠΈ Π·Π½Π°ΡΠΈΠΌΠΎΠ΅ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΠ΅ ΡΡΠΎΠ²Π½Π΅ΠΉ Π³Π»ΡΠΊΠΎΠ·Ρ ΠΈ Π³Π»ΠΈΠΊΠΈΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ Π³Π΅ΠΌΠΎΠ³Π»ΠΎΠ±ΠΈΠ½Π° Π½Π° Π½Π°ΡΠ°Π»ΡΠ½ΡΡ
ΡΡΠ°Π΄ΠΈΡΡ
Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ ΠΏΡΠΈ ΠΎΡΡΡΡΡΡΠ²ΠΈΠΈ ΡΠ°ΠΊΠΎΠ²ΠΎΠ³ΠΎ ΠΏΡΠΈ IV ΡΡΠ°Π΄ΠΈΠΈ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ. Π‘ΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠΈ Π·Π½Π°ΡΠΈΠΌΡΡ
ΠΎΡΠ»ΠΈΡΠΈΠΉ ΠΏΠΎ ΡΡΠΎΠ²Π½ΡΠΌ Π‘-ΠΏΠ΅ΠΏΡΠΈΠ΄Π° ΠΈ ΠΈΠΌΠΌΡΠ½ΠΎΡΠ΅Π°ΠΊΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΠΈΠ½ΡΡΠ»ΠΈΠ½Π° Π² ΠΊΡΠΎΠ²ΠΈ ΠΎΠ½ΠΊΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
Π±ΠΎΠ»ΡΠ½ΡΡ
Π²ΡΡΠ²Π»Π΅Π½ΠΎ Π½Π΅ Π±ΡΠ»ΠΎ, Ρ
ΠΎΡΡ ΠΈ Π½Π°Π±Π»ΡΠ΄Π°Π»Π°ΡΡ ΡΠ΅Π½Π΄Π΅Π½ΡΠΈΡ ΠΊ ΠΈΡ
ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΡ ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΠΎΠΉ Π³ΡΡΠΏΠΏΠΎΠΉ. ΠΡΠΈ Π°Π½Π°Π»ΠΈΠ·Π΅ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ ΠΌΠ°ΠΊΡΠΎΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ² ΡΠΆΠ΅ Π½Π° Π½Π°ΡΠ°Π»ΡΠ½ΡΡ
ΡΡΠ°Π΄ΠΈΡΡ
ΠΊΠ°Π½ΡΠ΅ΡΠΎΠ³Π΅Π½Π΅Π·Π° ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½ΠΎ ΡΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠΈ Π·Π½Π°ΡΠΈΠΌΠΎΠ΅ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΠ΅ ΡΡΠΎΠ²Π½Ρ Na Π² ΠΏΠ»Π°Π·ΠΌΠ΅ ΠΊΡΠΎΠ²ΠΈ, Π½Π΅ Π½Π°Π±Π»ΡΠ΄Π°ΡΡΠ΅Π΅ΡΡ ΠΏΡΠΈ ΡΠ΅ΡΠΌΠΈΠ½Π°Π»ΡΠ½ΠΎΠΉ ΡΡΠ°Π΄ΠΈΠΈ. ΠΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ K ΠΈ Π‘l ΠΈΠΌΠ΅Π΅Ρ ΡΠ΅Π½Π΄Π΅Π½ΡΠΈΡ ΠΊ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΡ Ρ ΠΎΠ½ΠΊΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
Π±ΠΎΠ»ΡΠ½ΡΡ
, Π½ΠΎ ΡΠ°Π·Π»ΠΈΡΠΈΡ ΡΡΠΈΡ
ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Π΅ΠΉ ΡΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠΈ Π½Π΅ Π·Π½Π°ΡΠΈΠΌΡ. ΠΡΠΈ Π·Π»ΠΎΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΡ
Π½ΠΎΠ²ΠΎΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡΡ
Π·Π½Π°ΡΠΈΠΌΠΎ ΠΏΠΎΠ²ΡΡΠ°Π΅ΡΡΡ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ Ca, Π , Mg. ΠΠ½Π°Π»ΠΈΠ· ΡΡΠΎΠ²Π½Ρ ΠΌΠΈΠΊΡΠΎΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ² Π² ΠΏΠ»Π°Π·ΠΌΠ΅ ΠΊΡΠΎΠ²ΠΈ ΠΏΠΎΠΊΠ°Π·Π°Π» ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΠ΅ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ Cu, Li (Π² 2,5β5 ΡΠ°Π·), ΡΠΎΡΡ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ Sr. ΠΠΈΡΠΈΠΉ ΠΎΠΊΠ°Π·ΡΠ²Π°Π΅Ρ ΠΌΠ½ΠΎΠΆΠ΅ΡΡΠ²Π΅Π½Π½ΡΠ΅ ΡΡΡΠ΅ΠΊΡΡ Π½Π° ΠΆΠΈΠ·Π½Π΅Π΄Π΅ΡΡΠ΅Π»ΡΠ½ΠΎΡΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ, Π²Π»ΠΈΡΡ Π½Π° ΡΡΠ΄ ΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ² ΡΠΈΡΡΠ΅ΠΌ ΠΌΠ΅ΡΡΠ΅Π½Π΄ΠΆΠ΅ΡΠΎΠ², Π° ΡΠ°ΠΊΠΆΠ΅ ΡΠ²Π»ΡΡΡΡ ΡΠΎΠΏΡΡΠ³Π°ΡΡΠΈΠΌ Π·Π²Π΅Π½ΠΎΠΌ ΠΌΠ΅ΠΆΠ΄Ρ ΡΠ³Π»Π΅Π²ΠΎΠ΄Π½ΡΠΌ ΠΎΠ±ΠΌΠ΅Π½ΠΎΠΌ ΠΈ ΠΌΠ°Π»ΠΈΠ³Π½ΠΈΠ·Π°ΡΠΈΠ΅ΠΉ ΠΊΠ»Π΅ΡΠΎΠΊ. ΠΠ°ΡΡΡΠ΅Π½ΠΈΠ΅ ΠΌΠΈΠ½Π΅ΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ Π³ΠΎΠΌΠ΅ΠΎΡΡΠ°Π·Π° ΡΠ²Π»ΡΠ΅ΡΡΡ Π·Π½Π°ΡΠΈΠΌΡΠΌ Π·Π²Π΅Π½ΠΎΠΌ Π² Π΄Π΅Π·ΠΈΠ½ΡΠ΅Π³ΡΠ°ΡΠΈΠΈ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΡΠΎΡΠ΅ΡΡΠΎΠ² ΠΏΡΠΈ ΠΊΠ°Π½ΡΠ΅ΡΠΎΠ³Π΅Π½Π΅Π·Π΅.
Π‘ΠΈΠ½Π΄ΡΠΎΠΌ VEXAS: Π½Π° ΡΡΠ±Π΅ΠΆΠ΅ ΡΠΌΠ΅Π½Ρ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½ΠΈΠΉ ΠΎΠ± ΠΈΠ·Π²Π΅ΡΡΠ½ΡΡ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡΡ
This article presents the first case of VEXAS syndrome identified in the Russian Federation as well as characteristics of currently known clinical manifestations and treatment approaches. The clinical observation described is an impressive example of how the identification of a new pathogenic mutation can change the understanding of the classification, diagnosis and treatment of previously known immunoinflammatory diseases. Thus, in refractory forms of relapsing polychondritis, neutrophilic dermatosis, atypical forms of vasculitis, inflammatory joint diseases or undifferentiated systemic inflammatory syndrome, especially when associated with macrocytic anemia and myelodysplastic syndrome, VEXAS syndrome should be suspected and genetic testing should be performed to exclude the autoinflammatory nature of the existing condition.Π ΡΡΠ°ΡΡΠ΅ ΠΏΡΠΈΠ²Π΅Π΄Π΅Π½ ΠΏΠ΅ΡΠ²ΡΠΉ ΡΠ»ΡΡΠ°ΠΉ ΡΠΈΠ½Π΄ΡΠΎΠΌΠ° VEXAS, Π²ΡΡΠ²Π»Π΅Π½Π½ΡΠΉ Π² Π ΠΎΡΡΠΈΠΉΡΠΊΠΎΠΉ Π€Π΅Π΄Π΅ΡΠ°ΡΠΈΠΈ, Π° ΡΠ°ΠΊΠΆΠ΅ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠ° ΠΈΠ·Π²Π΅ΡΡΠ½ΡΡ
Π½Π° Π½Π°ΡΡΠΎΡΡΠΈΠΉ ΠΌΠΎΠΌΠ΅Π½Ρ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΡΠΎΡΠ²Π»Π΅Π½ΠΈΠΉ ΠΈ ΠΏΠΎΠ΄Ρ
ΠΎΠ΄ΠΎΠ² ΠΊ Π΅Π³ΠΎ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ. ΠΠΏΠΈΡΠ°Π½Π½ΠΎΠ΅ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠ΅ Π½Π°Π±Π»ΡΠ΄Π΅Π½ΠΈΠ΅ ΡΠ²Π»ΡΠ΅ΡΡΡ ΡΡΠΊΠΈΠΌ ΠΏΡΠΈΠΌΠ΅ΡΠΎΠΌ ΡΠΎΠ³ΠΎ, ΠΊΠ°ΠΊ Π²ΡΡΠ²Π»Π΅Π½ΠΈΠ΅ Π½ΠΎΠ²ΠΎΠΉ ΠΏΠ°ΡΠΎΠ³Π΅Π½Π½ΠΎΠΉ ΠΌΡΡΠ°ΡΠΈΠΈ ΠΌΠΎΠΆΠ΅Ρ ΠΈΠ·ΠΌΠ΅Π½ΠΈΡΡ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½ΠΈΠ΅ ΠΎ ΠΊΠ»Π°ΡΡΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ, Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠ΅ ΠΈ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ ΡΠ°Π½Π΅Π΅ ΠΈΠ·Π²Π΅ΡΡΠ½ΡΡ
ΠΈΠΌΠΌΡΠ½ΠΎΠ²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΡΡ
Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ. Π’Π°ΠΊ, ΠΏΡΠΈ ΡΠ΅ΡΡΠ°ΠΊΡΠ΅ΡΠ½ΡΡ
ΡΠΎΡΠΌΠ°Ρ
ΡΠ΅ΡΠΈΠ΄ΠΈΠ²ΠΈΡΡΡΡΠ΅Π³ΠΎ ΠΏΠΎΠ»ΠΈΡ
ΠΎΠ½Π΄ΡΠΈΡΠ°, Π½Π΅ΠΉΡΡΠΎΡΠΈΠ»ΡΠ½ΠΎΠ³ΠΎ Π΄Π΅ΡΠΌΠ°ΡΠΎΠ·Π°, Π½Π΅ΡΠΈΠΏΠΈΡΠ½ΡΡ
ΡΠΎΡΠΌΠ°Ρ
Π²Π°ΡΠΊΡΠ»ΠΈΡΠ°, Π²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΡΡ
Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ ΡΡΡΡΠ°Π²ΠΎΠ² ΠΈΠ»ΠΈ Π½Π΅Π΄ΠΈΡΡΠ΅ΡΠ΅Π½ΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΌ ΡΠΈΡΡΠ΅ΠΌΠ½ΠΎΠΌ Π²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΠΎΠΌ ΡΠΈΠ½Π΄ΡΠΎΠΌΠ΅, ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎ ΠΏΡΠΈ Π°ΡΡΠΎΡΠΈΠ°ΡΠΈΠΈ Ρ ΠΌΠ°ΠΊΡΠΎΡΠΈΡΠ°ΡΠ½ΠΎΠΉ Π°Π½Π΅ΠΌΠΈΠ΅ΠΉ ΠΈ ΠΌΠΈΠ΅Π»ΠΎΠ΄ΠΈΡΠΏΠ»Π°ΡΡΠΈΡΠ΅ΡΠΊΠΈΠΌ ΡΠΈΠ½Π΄ΡΠΎΠΌΠΎΠΌ, Π½Π΅ΠΎΠ±Ρ
ΠΎΠ΄ΠΈΠΌΠ° Π½Π°ΡΡΠΎΡΠΎΠΆΠ΅Π½Π½ΠΎΡΡΡ Π² ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΠΈ ΡΠΈΠ½Π΄ΡΠΎΠΌΠ° VEXAS ΠΈ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΈΠ΅ Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π΄Π»Ρ ΠΈΡΠΊΠ»ΡΡΠ΅Π½ΠΈΡ Π°ΡΡΠΎΠ²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΠΎΠΉ ΠΏΡΠΈΡΠΎΠ΄Ρ ΠΈΠΌΠ΅ΡΡΠ΅Π³ΠΎΡΡ ΡΠΎΡΡΠΎΡΠ½ΠΈΡ
Comparison of printed glycan array, suspension array and ELISA in the detection of human anti-glycan antibodies
Anti-glycan antibodies represent a vast and yet insufficiently investigated subpopulation of naturally occurring and adaptive antibodies in humans. Recently, a variety of glycan-based microarrays emerged, allowing high-throughput profiling of a large repertoire of antibodies. As there are no direct approaches for comparison and evaluation of multi-glycan assays we compared three glycan-based immunoassays, namely printed glycan array (PGA), fluorescent microsphere-based suspension array (SA) and ELISA for their efficacy and selectivity in profiling anti-glycan antibodies in a cohort of 48 patients with and without ovarian cancer. The ABO blood group glycan antigens were selected as well recognized ligands for sensitivity and specificity assessments. As another ligand we selected P1, a member of the P blood group system recently identified by PGA as a potential ovarian cancer biomarker. All three glyco-immunoassays reflected the known ABO blood groups with high performance. In contrast, anti-P1 antibody binding profiles displayed much lower concordance. Whilst anti-P1 antibody levels between benign controls and ovarian cancer patients were significantly discriminated using PGA (pβ=β0.004), we got only similar results using SA (pβ=β0.03) but not for ELISA. Our findings demonstrate that whilst assays were largely positively correlated, each presents unique characteristic features and should be validated by an independent patient cohort rather than another array technique. The variety between methods presumably reflects the differences in glycan presentation and the antigen/antibody ratio, assay conditions and detection technique. This indicates that the glycan-antibody interaction of interest has to guide the assay selection
ΠΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠΈ Π½Π΅ΠΊΠΎΡΠΎΡΡΡ ΡΠΈΡΠΎΠΊΠΈΠ½ΠΎΠ² ΠΏΡΠΈ Π²ΠΈΡΡΡΠ½ΡΡ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΡΡ , ΠΏΡΠΎΡΠ΅ΠΊΠ°ΡΡΠΈΡ Ρ Π΄ΠΈΠ°ΡΠ΅ΠΉΠ½ΡΠΌ ΡΠΈΠ½Π΄ΡΠΎΠΌΠΎΠΌ Ρ Π΄Π΅ΡΠ΅ΠΉ
The aim of the study was to study the characteristics of the content of certain cytokines in serum as indicators of the immune response in viral infections accompanied by diarrhea syndrome in children. Materials and research methods. A survey was conducted of 92 children aged 3 months to 15 years with acute respiratory viral infections hospitalized in hospital No. 6 in Novosibirsk in the first 2 days from the onset of the disease. Two groups were formed β group 1 β patients with diarrheal syndrome and signs of respiratory system damage. Group 2 β children with isolated lesions of the respiratory tract. In all children, the genetic material of viruses was determined by polymerase chain reaction (PCR) in the nose and throat washes. Also, in all children, the determination of serum levels of IL-8, IL-17, IFN-Γ£ by ELISA was performed. Results and discussion. When studying the levels of cytokines (IFN-Γ£, IL-8, IL-17) in patients with signs of gastrointestinal tract lesions in comparison with isolated respiratory manifestations, no statistically significant differences were found. However, when analyzing the levels of the studied parameters in patients with diarrheal syndrome in different age groups, significant differences in the levels of IFN-Γ£ and IL-8 were revealed.Π¦Π΅Π»ΡΡ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΡΠ²ΠΈΠ»ΠΎΡΡ ΠΈΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠ΅ΠΉ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ Π½Π΅ΠΊΠΎΡΠΎΡΡΡ
ΡΠΈΡΠΎΠΊΠΈΠ½ΠΎΠ² Π² ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΠΊΡΠΎΠ²ΠΈ ΠΊΠ°ΠΊ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Π΅ΠΉ ΠΈΠΌΠΌΡΠ½Π½ΠΎΠ³ΠΎ ΡΠ΅Π°Π³ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΏΡΠΈ Π²ΠΈΡΡΡΠ½ΡΡ
ΠΈΠ½ΡΠ΅ΠΊΡΠΈΡΡ
, ΡΠΎΠΏΡΠΎΠ²ΠΎΠΆΠ΄Π°ΡΡΠΈΡ
ΡΡ Π΄ΠΈΠ°ΡΠ΅ΠΉΠ½ΡΠΌ ΡΠΈΠ½Π΄ΡΠΎΠΌΠΎΠΌ Ρ Π΄Π΅ΡΠ΅ΠΉ. ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ. ΠΡΠΎΠ²Π΅Π΄Π΅Π½ΠΎ ΠΎΠ±ΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ 92 Π΄Π΅ΡΠ΅ΠΉ Π² Π²ΠΎΠ·ΡΠ°ΡΡΠ΅ ΠΎΡ 3 ΠΌΠ΅ΡΡΡΠ΅Π² Π΄ΠΎ 15 Π»Π΅Ρ Ρ ΠΎΡΡΡΡΠΌΠΈ ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΡΠΌΠΈ Π²ΠΈΡΡΡΠ½ΡΠΌΠΈ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΡΠΌΠΈ, Π³ΠΎΡΠΏΠΈΡΠ°Π»ΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
Π² ΡΡΠ°ΡΠΈΠΎΠ½Π°Ρ ΠΠΠ β6 Π³ΠΎΡΠΎΠ΄Π° ΠΠΎΠ²ΠΎΡΠΈΠ±ΠΈΡΡΠΊΠ° Π² ΠΏΠ΅ΡΠ²ΡΠ΅ Π΄Π²ΠΎΠ΅ ΡΡΡΠΎΠΊ ΠΎΡ Π½Π°ΡΠ°Π»Π° Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ. Π‘ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½Ρ Π΄Π²Π΅ Π³ΡΡΠΏΠΏΡ: I Π³ΡΡΠΏΠΏΠ° β ΠΏΠ°ΡΠΈΠ΅Π½ΡΡ Ρ Π΄ΠΈΠ°ΡΠ΅ΠΉΠ½ΡΠΌ ΡΠΈΠ½Π΄ΡΠΎΠΌΠΎΠΌ ΠΈ ΠΏΡΠΈΠ·Π½Π°ΠΊΠ°ΠΌΠΈ ΠΏΠΎΡΠ°ΠΆΠ΅Π½ΠΈΡ ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΡ. ΠΡΡΠΏΠΏΠ° II β Π΄Π΅ΡΠΈ Ρ ΠΈΠ·ΠΎΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΡΠΌ ΠΏΠΎΡΠ°ΠΆΠ΅Π½ΠΈΠ΅ΠΌ ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΠΎΠ³ΠΎ ΡΡΠ°ΠΊΡΠ°. Π£ Π²ΡΠ΅Ρ
Π΄Π΅ΡΠ΅ΠΉ ΠΏΡΠΎΠ²Π΅Π»ΠΈ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Π° Π²ΠΈΡΡΡΠΎΠ² ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠ°Π·Π½ΠΎΠΉ ΡΠ΅ΠΏΠ½ΠΎΠΉ ΡΠ΅Π°ΠΊΡΠΈΠΈ (ΠΠ¦Π ) Π² ΡΠΌΡΠ²Π°Ρ
ΠΈΠ· Π½ΠΎΡΠΎ- ΠΈ ΡΠΎΡΠΎΠ³Π»ΠΎΡΠΊΠΈ. Π’Π°ΠΊΠΆΠ΅ Ρ Π²ΡΠ΅Ρ
Π΄Π΅ΡΠ΅ΠΉ Π±ΡΠ»ΠΎ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΎ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ Π² ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΠΊΡΠΎΠ²ΠΈ IL-8, IL-17, IFN-Γ£ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΠ€Π. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΈ ΠΎΠ±ΡΡΠΆΠ΄Π΅Π½ΠΈΠ΅. ΠΡΠΈ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΈ ΡΡΠΎΠ²Π½Π΅ΠΉ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ ΡΠΈΡΠΎΠΊΠΈΠ½ΠΎΠ² (IFN-Γ£, IL-8, IL-17) Ρ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ ΠΏΡΠΈΠ·Π½Π°ΠΊΠ°ΠΌΠΈ ΠΏΠΎΡΠ°ΠΆΠ΅Π½ΠΈΡ ΠΆΠ΅Π»ΡΠ΄ΠΎΡΠ½ΠΎ-ΠΊΠΈΡΠ΅ΡΠ½ΠΎΠ³ΠΎ ΡΡΠ°ΠΊΡΠ° Π² ΡΡΠ°Π²Π½Π΅Π½ΠΈΠΈ Ρ ΠΈΠ·ΠΎΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΡΠΌΠΈ ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΡΠΌΠΈ ΠΏΡΠΎΡΠ²Π»Π΅Π½ΠΈΡΠΌΠΈ ΡΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠΈ Π·Π½Π°ΡΠΈΠΌΡΡ
ΡΠ°Π·Π»ΠΈΡΠΈΠΉ Π²ΡΡΠ²Π»Π΅Π½ΠΎ Π½Π΅ Π±ΡΠ»ΠΎ. ΠΠ΄Π½Π°ΠΊΠΎ ΠΏΡΠΈ Π°Π½Π°Π»ΠΈΠ·Π΅ ΡΡΠΎΠ²Π½Π΅ΠΉ ΠΈΡΡΠ»Π΅Π΄ΡΠ΅ΠΌΡΡ
ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Π΅ΠΉ Ρ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ Π΄ΠΈΠ°ΡΠ΅ΠΉΠ½ΡΠΌ ΡΠΈΠ½Π΄ΡΠΎΠΌΠΎΠΌ Π² ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
Π²ΠΎΠ·ΡΠ°ΡΡΠ½ΡΡ
Π³ΡΡΠΏΠΏΠ°Ρ
Π²ΡΡΠ²Π»Π΅Π½Ρ Π΄ΠΎΡΡΠΎΠ²Π΅ΡΠ½ΡΠ΅ ΡΠ°Π·Π»ΠΈΡΠΈΡ Π² ΡΡΠΎΠ²Π½Π΅ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ IFN-Γ£ ΠΈ IL-8
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