“We’re just stuck in a daily routine”:Implications of the temporal dimensions, demands and dispositions of mothering for leisure time physical activity

The reduced physical activity of women when they become mothers is a public health priority. Existing studies show that mothers have little time for leisure, or time that is fragmented and requiring negotiation with others. However, the temporal features of mothering are undertheorised and qualitative studies tend to focus on how mothers can skilfully construct physically active identities and balance societal expectations about being a "good mother". In line with other research that focuses on the configuration of everyday practices that condition the "possibilities" for health-related practices like physical activity, we shift our focus away from the resisting capacities of mothers to the temporal features of mothering practices. We interrogate the lived experiences of 15 mothers of preschool children in deprived urban areas and illuminate the inherent temporal dimensions, demands and dispositions of mothering practices that condition the possibility of leisure time physical activity being undertaken. Together, these temporal features mean mothering practices can readily work against leisure time physical activity. The focus on the mothering practices rather than mothers brings a novel perspective for developing public health policy designed to support mothers into regular leisure time physical activity

Characterizing RecA-Independent Induction of Shiga toxin2-Encoding Phages by EDTA Treatment

Background: The bacteriophage life cycle has an important role in Shiga toxin (Stx) expression. The induction of Shiga toxin-encoding phages (Stx phages) increases toxin production as a result of replication of the phage genome, and phage lysis of the host cell also provides a means of Stx toxin to exit the cell. Previous studies suggested that prophage induction might also occur in the absence of SOS response, independently of RecA. Methodology/Principal Findings: The influence of EDTA on RecA-independent Stx2 phage induction was assessed, in laboratory lysogens and in EHEC strains carrying Stx2 phages in their genome, by Real-Time PCR. RecA-independent mechanisms described for phage l induction (RcsA and DsrA) were not involved in Stx2 phage induction. In addition, mutations in the pathway for the stress response of the bacterial envelope to EDTA did not contribute to Stx2 phage induction. The effect of EDTA on Stx phage induction is due to its chelating properties, which was also confirmed by the use of citrate, another chelating agent. Our results indicate that EDTA affects Stx2 phage induction by disruption of the bacterial outer membrane due to chelation of Mg 2+. In all the conditions evaluated, the pH value had a decisive role in Stx2 phage induction. Conclusions/Significance: Chelating agents, such as EDTA and citrate, induce Stx phages, which raises concerns due to their frequent use in food and pharmaceutical products. This study contributes to our understanding of the phenomenon o

Type II Heat-Labile Enterotoxins from 50 Diverse Escherichia coli Isolates Belong Almost Exclusively to the LT-IIc Family and May Be Prophage Encoded

Some enterotoxigenic Escherichia coli (ETEC) produce a type II heat-labile enterotoxin (LT-II) that activates adenylate cyclase in susceptible cells but is not neutralized by antisera against cholera toxin or type I heat-labile enterotoxin (LT-I). LT-I variants encoded by plasmids in ETEC from humans and pigs have amino acid sequences that are ≥95% identical. In contrast, LT-II toxins are chromosomally encoded and are much more diverse. Early studies characterized LT-IIa and LT-IIb variants, but a novel LT-IIc was reported recently. Here we characterized the LT-II encoding loci from 48 additional ETEC isolates. Two encoded LT-IIa, none encoded LT-IIb, and 46 encoded highly related variants of LT-IIc. Phylogenetic analysis indicated that the predicted LT-IIc toxins encoded by these loci could be assigned to 6 subgroups. The loci corresponding to individual toxins within each subgroup had DNA sequences that were more than 99% identical. The LT-IIc subgroups appear to have arisen by multiple recombinational events between progenitor loci encoding LT-IIc1- and LT-IIc3-like variants. All loci from representative isolates encoding the LT-IIa, LT-IIb, and each subgroup of LT-IIc enterotoxins are preceded by highly-related genes that are between 80 and 93% identical to predicted phage lysozyme genes. DNA sequences immediately following the B genes differ considerably between toxin subgroups, but all are most closely related to genomic sequences found in predicted prophages. Together these data suggest that the LT-II loci are inserted into lambdoid type prophages that may or may not be infectious. These findings raise the possibility that production of LT-II enterotoxins by ETEC may be determined by phage conversion and may be activated by induction of prophage, in a manner similar to control of production of Shiga-like toxins by converting phages in isolates of enterohemmorhagic E. coli

Accounting for success and failure: a discursive psychological approach to sport talk

YesIn recent years, constructionist methodologies such as discursive psychology (Edwards & Potter, 1992) have begun to be used in sport research. This paper provides a practical guide to applying a discursive psychological approach to sport data. It discusses the assumptions and principles of discursive psychology and outlines the stages of a discursive study from choice of data through to transcription and analysis. Finally, the paper demonstrates a discursive psychological analysis on sport data where athletes are accounting for success and failure in competition. The analysis demonstrates that for both success and failure, there is an apparent dilution of personal agency, to either maintain their modesty in the case of success or to manage blame when talking about failure. It is concluded that discursive psychology has much to offer sport research as it provides a methodology for in-depth studies of supporting interactions

Can workplace active living work ? Perspectives from the workplace

Promotion de l'activité physique dans les entreprises et autres milieux professionnels au Canada. Enquête auprès des intervenants institutionnels de cette campagne de promotion (responsables hygiène et sécurité, assistantes sociales, responsables ressources humaines...) sur le thème de la 'vie active au travail'

Negotiating a coach identity : a theoretical critique of elite athletes’ transitions into post-athletic high-performance coaching roles

There has been a growing trend of elite athletes being fast-tracked into post-athletic high-performance coaching roles in association football and rugby union in England and Wales. This has been facilitated by an increase in bespoke and condensed formal coach education courses that are designed to accelerate current and/or former elite athletes in attaining their coaching accreditation. Hitherto, however, the individual lived experiences of former athletes on this career trajectory during their transition to coaching remains under-investigated. The purpose of this study, therefore, was to analyse how elite male association football and rugby union athletes based in the England and Wales (re)created, re-negotiated or transformed their identities when negotiating a fast-tracked career pathway into a post-athletic high-performance coaching role. Fifteen male rugby union (n=10) and association football (n=5) athletes were interviewed on two separate occasions over twelve months. Interviews coincided with the start and end dates of the level three coach education course which they were concurrently enrolled on. Interviews focused upon how they (re)created their professional identities upon negotiating the career transition into a post-athletic high-performance coaching role. Data were critically theorised against sociological concepts associated to the theoretical frameworks of Bourdieu, Goffman and Foucault. Results identified how the development of a coaching identity was articulated through the need to define a ‘coaching philosophy’. Upholding a coach identity in an ‘honest’ disposition so athlete to coach respect could be best attained was expressed by all participants and contrasted with Goffman’s concepts of front and back stage impression management. Self-reflexive practices of Foucault’s askesis were engaged to varying levels to create a coach identity. In three cases this resulted in participants contestation of their respective club’s identity/culture and losing employment as a coach. Finally, recommendations on how coach education structures can further support these coaches in their career transitions are made