Impaired Intracellular Transport and Cell Surface Expression of Nonpolymorphic HLA-E

The assembly of the classical, polymorphic major histocompatibility complex class I molecules in the endoplasmic reticulum requires the presence of peptide ligands and ~2-microglobulin (~2m). Formation of this trimolecular complex is a prerequisite for e~cient transport to the cell surface, where presented peptides are scanned by T lymphocytes. The function of the other class I molecules is in dispute. The human, nonclassical class I gene, HLA-E, was found to be ubiquitously transcribed, whereas cell surface expression was dif~cult to detect upon transfection. Pulse chase experiments revealed that the HLA-E heavy chain in transfectants, obtained with the murine myeloma cell line P3X63-Ag8.653 (X63), displays a significant reduction in oligosaccharide maturation and intracellular transport compared with HLA-B27 in corresponding transfectants. The accordingly low HLA-E cell surface expression could be significantly enhanced by either reducing the culture temperature or by supplementing the medium with human ~2m, suggesting inefficient binding of endogenous peptides to HLA-E. To analyze whether HLA-E binds peptides and to identify the corresponding ligands, fractions of acid-extracted material from HLA-E/X63 transfectants were separated by reverse phase HPLC and were tested for their ability to enhance HLA-E cell surface expression. Two fractions specifically increased the HLA class I expression on the HLA-E transfectant clone

Radio faint AGN: a tale of two populations

We study the Extended Chandra Deep Field South (E-CDFS) Very Large Array sample, which reaches a flux density limit at 1.4 GHz of 32.5 microJy at the field centre and redshift ~ 4, and covers ~ 0.3 deg^2. Number counts are presented for the whole sample while the evolutionary properties and luminosity functions are derived for active galactic nuclei (AGN). The faint radio sky contains two totally distinct AGN populations, characterised by very different evolutions, luminosity functions, and Eddington ratios: radio-quiet (RQ)/radiative-mode, and radio-loud/jet-mode AGN. The radio power of RQ AGN evolves ~ (1+z)^2.5, similarly to star-forming galaxies, while the number density of radio-loud ones has a peak at ~ 0.5 and then declines at higher redshifts. The number density of radio-selected RQ AGN is consistent with that of X-ray selected AGN, which shows that we are sampling the same population. The unbiased fraction of radiative-mode RL AGN, derived from our own and previously published data, is a strong function of radio power, decreasing from ~ 0.5 at P_1.4GHz ~ 10^24 W/Hz to ~ 0.04$ at P_1.4GHz ~ 10^22 W/Hz. Thanks to our enlarged sample, which now includes ~ 700 radio sources, we also confirm and strengthen our previous results on the source population of the faint radio sky: star-forming galaxies start to dominate the radio sky only below ~ 0.1 mJy, which is also where radio-quiet AGN overtake radio-loud ones.Comment: 19 pages, 13 figures, accepted for publication in MNRA

Multi-frequency investigation of the parsec- and kilo-parsec-scale radio structures in high-redshift quasar PKS 1402+044

We investigate the frequency-dependent radio properties of the jet of the luminous high-redshift (z = 3.2) radio quasar PKS 1402+044 (J1405+0415) by means of radio interferometric observations. The observational data were obtained with the VLBI Space Observatory Programme (VSOP) at 1.6 and 5 GHz, supplemented by other multi-frequency observations with the Very Long Baseline Array (VLBA; 2.3, 8.4, and 15 GHz) and the Very Large Array (VLA; 1.4, 5, 15, and 43 GHz). The observations span a period of 7 years. We find that the luminous high-redshift quasar PKS 1402+044 has a pronounced "core-jet" morphology from the parsec to the kilo-parsec scales. The jet shows a steeper spectral index and lower brightness temperature with increasing distance from the jet core. The variation of brightness temperature agrees well with the shock-in-jet model. Assuming that the jet is collimated by the ambient magnetic field, we estimate the mass of the central object as ~10^9 M_sun. The upper limit of the jet proper motion of PKS 1402+044 is 0.03 mas/yr (~3c) in the east-west direction.Comment: 9 pages, 6 figures

The sub-mJy radio sky in the Extended Chandra Deep Field South: source population

The sub-mJy radio population is a mixture of active systems, that is star forming galaxies (SFGs) and active galactic nuclei (AGNs). We study a sample of 883 radio sources detected at 1.4 GHz in a deep Very Large Array survey of the Extended Chandra Deep Field South (E-CDFS) that reaches a best rms sensitivity of 6 microJy. We have used a simple scheme to disentangle SFGs, radio-quiet (RQ), and radio-loud (RL) AGNs based on the combination of radio data with Chandra X-ray data and mid-infrared observations from Spitzer. We find that at flux densities between about 30 and 100 microJy the radio population is dominated by SFGs (~60%) and that RQ AGNs become increasingly important over RL ones below 100 microJy. We also compare the host galaxy properties of the three classes in terms of morphology, optical colours and stellar masses. Our results show that both SFG and RQ AGN host galaxies have blue colours and late type morphology while RL AGNs tend to be hosted in massive red galaxies with early type morphology. This supports the hypothesis that radio emission in SFGs and RQ AGNs mainly comes from the same physical process: star formation in the host galaxy.Comment: 13 pages, 11 figures, 1 table, accepted for publication in MNRA

The micro-Jy Radio Source Population: the VLA-CDFS View

We analyse the 267 radio sources from our deep (flux limit of 42 microJy at the field center at 1.4 GHz) Chandra Deep Field South 1.4 and 5 GHz VLA survey. The radio population is studied by using a wealth of multi-wavelength information, including morphology and spectral types, in the radio, optical, and X-ray bands. The availability of redshifts for ~ 70% of our sources allows us to derive reliable luminosity estimates for the majority of the objects. Contrary to some previous results, we find that star-forming galaxies make up only a minority (~ 1/3) of sub-mJy sources, the bulk of which are faint radio galaxies, mostly of the Fanaroff-Riley I type.Comment: 6 pages, 3 figures, to appear in the proceedings of "At the Edge of the Universe", Sintra, Portugal, Oct. 9 - 13, 200

The Parkes quarter-Jansky flat-spectrum sample 3. Space density and evolution of QSOs

We analyze the Parkes quarter-Jansky flat-spectrum sample of QSOs in terms of space density, including the redshift distribution, the radio luminosity function, and the evidence for a redshift cutoff. With regard to the luminosity function, we note the strong evolution in space density from the present day to epochs corresponding to redshifts ~ 1. We draw attention to a selection effect due to spread in spectral shape that may have misled other investigators to consider the apparent similarities in shape of luminosity functions in different redshift shells as evidence for luminosity evolution. To examine the evolution at redshifts beyond 3, we develop a model-independent method based on the V_max test using each object to predict expectation densities beyond z=3. With this we show that a diminution in space density at z > 3 is present at a significance level >4 sigma. We identify a severe bias in such determinations from using flux-density measurements at epochs significantly later than that of the finding survey. The form of the diminution is estimated, and is shown to be very similar to that found for QSOs selected in X-ray and optical wavebands. The diminution is also compared with the current estimates of star-formation evolution, with less conclusive results. In summary we suggest that the reionization epoch is little influenced by powerful flat-spectrum QSOs, and that dust obscuration does not play a major role in our view of the QSO population selected at radio, optical or X-ray wavelengths.Comment: 18 pages, 11 figures, accepted 18 Dec 2004, Astron. & Astrophys. The accepted version is expanded to include an analysis of the form of the decline in radio-QSO space density at high redshifts. This is compared with the forms of epoch dependence derived for optically-selected QSOs, for X-ray-selected QSOs, and for star formation rat