Restriction fragment length polymorphisms of different DNA regions as genetic markers in the hoverfly Episyrphus balteatus (Diptera: Syrphidae)

A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis using mitochondrial (A + T-rich region; mtDNA) and genomic (zen-region; nDNA) DNA was performed on 182 female individuals of Episyrphus balteatus (DeGeer), a widespread aphidophagous hoverfly with supposed migratory behaviour. Specimens originated from 13 sampling sites in six European countries. The analyses revealed 12 and 18 haplotypes, respectively, for the two DNA types, several of them with a wide distribution, although seven and eight haplotypes, respectively, occurred only in one location. In contrast to other studies on mobile insects, the genetic diversity was relatively high. However, lack of population subdivision, low genetic distances between populations, the very high gene flow rates, and the complete lack of isolation by distance suggest that E. balteatus populations are largely connected and that there is an absence of large-scale geographic structuring. These results support the hypothesis that E. balteatus is a migratory hoverfly species, capable of moving over large distances. These findings related to the seasonal migrations of this species are discussed. © CAB International, 2005

Exceeding the threshold value for Trioza apicalis Förster 1848 in carrot fields did not cause damage as revealed during monitoring in Germany from 2017–2020

The carrot psyllid Trioza apicalis Förster 1848 is a carrot pest in Europe that can cause serious damages in case of massive occurrence. Damages up to a total loss of yield have been reported from Scandinavian countries but also from Switzerland. The action threshold to control the pest with chemical pesticides is 0.2 T. apicalis per day and trap caught by sticky traps. We investigated the number of T. apicalis with sticky traps on carrot fields of the study regions Lüneburg/Uelzen and Hameln/Bad Pyrmont in Germany, during the period 2017–2020. The number of T. apicalis caught was generally very low in both study regions. On several fields in successive weeks almost no individuals were found in the study region Hameln/Bad Pyrmont. In Lüneburg/Uelzen was at least one field each year where the number of carrot psyllid was clearly higher than in all other fields and exceeded the threshold level. Surprisingly on carrot fields in close proximity to carrot fields from the previous year, the T. apicalis numbers were only slightly increased. Nonetheless, no loss of yield was reported for any of the fields in the four years of the study, although the generally defined threshold has been exceeded on many of the investigated carrot fields

Exceeding the threshold value for Trioza apicalis Förster 1848 in carrot fields did not cause damage as revealed during monitoring in Germany from 2017–2020

The carrot psyllid Trioza apicalis Förster 1848 is a carrot pest in Europe that can cause serious damages in case of massive occurrence. Damages up to a total loss of yield have been reported from Scandinavian countries but also from Switzerland. The action threshold to control the pest with chemical pesticides is 0.2 T. apicalis per day and trap caught by sticky traps. We investigated the number of T. apicalis with sticky traps on carrot fields of the study regions Lüneburg/Uelzen and Hameln/Bad Pyrmont in Germany, during the period 2017–2020. The number of T. apicalis caught was generally very low in both study regions. On several fields in successive weeks almost no individuals were found in the study region Hameln/Bad Pyrmont. In Lüneburg/Uelzen was at least one field each year where the number of carrot psyllid was clearly higher than in all other fields and exceeded the threshold level. Surprisingly on carrot fields in close proximity to carrot fields from the previous year, the T. apicalis numbers were only slightly increased. Nonetheless, no loss of yield was reported for any of the fields in the four years of the study, although the generally defined threshold has been exceeded on many of the investigated carrot fields.Bundesanstalt für Landwirtschaft und Ernährung http://dx.doi.org/10.13039/501100010771Bundesanstalt für Landwirtschaft und Ernährung (DE)Julius Kühn-Institut (JKI), Bundesforschungsinstitut für Kulturpflanzen (4250)Peer Reviewe

Genetic structure and diversity of western flower thrips, Frankliniella occidentalis in a French bean agroecosystem of Kenya

Western flower thrips (WFT) (Frankliniella occidentalis) is an introduced pest that harms French bean production in Kenya and other countries. Since new WFT management approaches are being developed, a closer look at the genetic makeup of WFT populations can give new insights into source habitats, crop colonization patterns or host plant preferences, which are prerequisites for integrated pest management (IPM) strategies. For this purpose, we used six microsatellite loci to analyse the genetic structure, diversity and gene flow of WFT sampled on French beans, intercrops and weeds in Kenyan French bean production areas. The results of this preliminary study indicate that the available microsatellites are sufficiently polymorphic for more detailed analyses on local dispersal patterns of WFT in Kenya. Even with the limited data set, the results reveal that F. occidentalis populations show considerable genetic differentiation between host plant species but not between regions, which suggests reduced gene flow and a possible development of biotypes. Possible consequences of the results on IPM are discussed. Copyright © icipe 201

Are primary woody hosts 'island refuges' for host-alternating aphids and important for colonization of local cereals?

International audienceOnly few studies are available dealing with the relation between winter host density and spatial distribution and spring colonization of winter cereals by the host-alternating cereal aphid species Rhopalosiphum padi and Metopolophium dirhodum. Large-scale studies in climatically different agroecosystems in Germany from 2004 to 2006 revealed for R.padi and M.dirhodum larger spring/summer populations in landscapes with higher densities of winter hosts. A small-scale study was performed in winter wheat fields adjacent to a large hedge with several typical winter hosts plants, bird cherry (Prunus padus) and wild rose species (Rosa spp.) to indentify distance effects (08, 824 and 2460 m). Weekly measurements of aphid density between May to July showed significantly higher densities of R.padi compared with those of other aphids. Statistical analysis (TukeyKramer test and regression analyses) revealed significant gradients from the hedge to the field centre for R.padi and M.dirhodum. In comparative studies, winged R.padi from winter and adjacent summer hosts were genotyped using four microsatellite markers. The results showed that individuals from a certain winter host were not genetically similar with individuals from neighbouring summer hosts; it, therefore, seems that winter host clones did not significantly contribute to population built-up in cereal fields over short distances. It could be concluded that on a regional scale, the density of sources for early migrants of R.padi is important for colonization intensity of surrounding summer hosts, but that the high local movement intensity and the relative small proportion of aphids that could be analysed in such tracking studies are blurring close spatial relations within short time periods

Higher flight activity in the offspring of migrants compared to residents in a migratory insect

Migration has evolved among many animal taxa and migratory species are found across all major lineages. Insects are the most abundant and diverse terrestrial migrants, with trillions of animals migrating annually. Partial migration, where populations consist of resident and migratory individuals, is ubiquitous among many taxa. However, the underlying mechanisms are relatively poorly understood and may be driven by physiological, behavioural or genetic variation within populations. We investigated the differences in migratory tendency between migratory and resident phenotypes of the hoverfly, Episyrphus balteatus, using tethered flight mills. Further, to test whether migratory flight behaviour is heritable and to disentangle the effects of environment during development, we compared the flight behaviour of laboratory-reared offspring of migrating, overwintering and summer animals. Offspring of migrants initiated more flights than those of resident individuals. Interestingly, there were no differences among wild-caught phenotypes with regard to number of flights or total flight duration. Low activity in field-collected migrants might be explained by an energy-conserving state that migrants enter into when under laboratory conditions, or a lack of suitable environmental cues for triggering migration. Our results strongly suggest that flight behaviour is heritable and that genetic factors influence migratory tendency in E. balteatus. These findings support the growing evidence that genetic factors play a role in partial migration and warrant careful further investigation