77 research outputs found
A systematic review and meta-analysis of the use of renin-angiotensin system drugs and COVID-19 clinical outcomes : What is the evidence so far?
Peer reviewedPublisher PD
Statins: Could an old friend help the fight against COVID-19?
This is the peer reviewed version of the following article: "Statins: Could an old friend help the fight against COVID-19?" . British Journal of Pharmacology (2020): 19 June, which has been published in final form at https://doi.org/10.1111/bph.15166. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versionshe COVID-19 pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has overwhelmed healthcare systems requiring the rapid development of treatments, at least, to reduce COVID-19 severity. Drug repurposing offers a fast track. Here, we discuss the potential beneficial effects of statins in COVID-19 patients based on evidence that they may target virus receptors, replication, degradation, and downstream responses in infected cells, addressing both basic research and epidemiological information. Briefly, statins could modulate virus entry, acting on the SARS-CoV-2 receptors, ACE2 and CD147, and/or lipid rafts engagement. Statins, by inducing autophagy activation, could regulate virus replication or degradation, exerting protective effects. The well-known anti-inflammatory properties of statins, by blocking several molecular mechanisms, including NF-ÎșB and NLRP3 inflammasomes, could limit the "cytokine storm" in severe COVID-19 patients which is linked to fatal outcome. Finally, statin moderation of coagulation response activation may also contribute to improving COVID-19 outcomesThis work and data discussed here were supported by grants from the Instituto de
Salud Carlos III (ISCIII) and Fondos FEDER European Union (PI17/00119 and Red de
InvestigaciĂłn Renal (REDINREN): RD16/0009, to M.R-O, PI17/01495 to J.E, PI18/01133 to
AMR, PI19/00815 to A.O); Comunidad de Madrid (âNOVELRENâ B2017/BMD3751
to
M.R-O, B2017/BMD-3686 CIFRA2-CM to A.O); Spanish Ministry of Economy and
Competitiveness MINECO (DTS17/00203, DTS19/00093) to J,E; âConvocatoria
DinamizaciĂłn Europa InvestigaciĂłn 2019â MINECO (EIN2019-103294 to M.R-O and SR-M);
ERA-PerMed-JTC2018 (KIDNEY ATTACK AC18/00064 and PERSTIGAN AC18/00071)
and DTS18/00032 to A.O; The âSara Borrellâ postdoctoral training program of the ISCIII
supported the salary of SR-M (CD19/00021), IMPROVE-PD project (âIdentification and
Management of Patients at RiskâOutcome and Vascular Events in Peritoneal Dialysisâ)
funding from the European Unionâs Horizon 2020 research and innovation program under the
Marie SkĆodowska-Curie Grant Agreement No. 812699 to M.R.O
T Cells Recognizing a Peptide Contaminant Undetectable by Mass Spectrometry
Synthetic peptides are widely used in immunological research as epitopes to stimulate their cognate T cells. These preparations are never completely pure, but trace contaminants are commonly revealed by mass spectrometry quality controls. In an effort to characterize novel major histocompatibility complex (MHC) Class I-restricted ÎČ-cell epitopes in non-obese diabetic (NOD) mice, we identified islet-infiltrating CD8+ T cells recognizing a contaminating peptide. The amount of this contaminant was so small to be undetectable by direct mass spectrometry. Only after concentration by liquid chromatography, we observed a mass peak corresponding to an immunodominant islet-specific glucose-6-phosphatase catalytic subunit-related protein (IGRP)206-214 epitope described in the literature. Generation of CD8+ T-cell clones recognizing IGRP206-214 using a novel method confirmed the identity of the contaminant, further underlining the immunodominance of IGRP206-214. If left undetected, minute impurities in synthetic peptide preparations may thus give spurious results
Reactive oxygen species generation by bovine blood neutrophils with different CXCR1 (IL8RA) genotype following Interleukin-8 incubation
Background: Associations between polymorphisms in the bovine CXCR1 gene, encoding the chemokine (C-X-C motif) receptor 1 (IL8RA), and neutrophil traits and mastitis have been described. In the present study, blood neutrophils were isolated from 20 early lactating heifers with different CXCR1 genotype at position 735 or 980. The cells were incubated with different concentrations of recombinant bovine IL-8 (rbIL-8) for 2 or 6 h and stimulated with phorbol 12-myristate 13-acetate (PMA) or opsonized zymosan particles (OZP). Potential association between CXCR1 genotype and production of reactive oxygen species (ROS) was studied.
Results: Although on single nucleotide polymorphisms (SNPs) may potentially affect CXCR1 function, SNPs c.735C > G and c.980A > G showed no association with ROS production with or without incubation of rbIL-8. Neutrophils incubated with rbIL-8 for 2 or 6 h showed higher PMA- and lower OZP-induced ROS production compared to control without rbIL-8.
Conclusions: In the present study no association could be detected between superoxide production by isolated bovine neutrophils during early lactation and CXCR1 gene polymorphism. IL-8 showed to possess inhibitory effects on ROS generation in bovine neutrophils
Cov-MS: A Community-Based Template Assay for Mass-Spectrometry-Based Protein Detection in SARS-CoV-2 Patients
Reactive oxygen species generation by bovine blood neutrophils with different CXCR1 (IL8RA) genotype following Interleukin-8 incubation
Quantitative analysis of cis-dichlorodiammineplatinum(II) by high-performance thin-layer chromatography
A strategy for two-dimensional, high-performance thin-layer chromatography, applied to local anesthetics
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