Dogwood Borer (Lepidoptera: Sesiidae) Abundance and Seasonal Flight Activity in Apple Orchards, Urban Landscapes, and Woodlands in Five Eastern States

The relative abundance and seasonal flight activity of dogwood borer, Synanthedon scitula Harris (Lepidoptera: Sesiidae), was measured using weekly records from traps baited with its sex pheromone and deployed in apple orchards, urban landscapes, and native woodland sites in New York, West Virginia, Virginia, North Carolina, and Tennessee in 2005 and 2006. The mean total number of moths captured per site in apple orchards was 3,146 ± 644 and 3095 ± 584 SE in 2005 and 2006, respectively, exceeding captures at urban sites by 16 and 13 times and at woodland sites by 210 and 206 times in 2005 and 2006, respectively. Mean total captures at urban sites exceeded those in woodland habitats by 13 and 16 times in 2005 and 2006, respectively. The mean duration (wk) of the flight period did not differ significantly between apple orchards (22.6 ± 0.6 SE) and urban sites (20.3 ± 1.2 SE). The onset of flight was somewhat later in New York (around early June) than further south (around early to mid-May), but moth captures continued into October in all states. Captures in apple orchards and at urban sites with higher populations were essentially continuous throughout the flight period, with substantial weekly fluctuations, and tended to show a bimodal pattern with peaks from late May through mid-July and from late August through mid-September. Captures at woodland sites tended to occur predominantly from mid-May through about mid-June and were very sporadic thereafte

Seasonal Flight Activity and Distribution of Metallic Woodboring Beetles (Coleoptera: Buprestidae) Collected in North Carolina and Tennessee

Distribution records and seasonal flight activity information for metallic woodboring beetle (Coleoptera: Buprestidae) species have not been compiled for North Carolina and Tennessee. Institutional, research, and private collections in North Carolina and Tennessee were reviewed to provide seasonal activity data of 5 subfamilies of buprestid beetle species. Label information was checked for 15,217 specimens of 135 species collected between 1901 and 2013 (North Carolina) and between 1934 and 2013 (Tennessee). These collections provided data on adult seasonal activity and county records for 121 species (4,467 specimens) and 105 species (10,750 specimens) from North Carolina and Tennessee, respectively. Two species, Agrilus carpini Knull and A. pensus Horn, are reported as New State Records for North Carolina. The data reveal key geographic areas in both states where few to no collections have been made, highlighting opportunities to validate species distributions and locations where future collecting efforts can be matched with the occurrence of larval and adult host plant resources. Seasonal activity records will inform future biosurveillance efforts for invasive and endemic pests and facilitate predictions of buprestid species that are likely to be active within the hunting flight season of Cerceris fumipennis (Say) (Hymenoptera: Crabronidae) wasps. Activity periods of the buprestids also can focus the management of selected economic pest species to times of the year when treatment efforts, particularly through use of contact insecticides, are likely to be most effective

Small and mighty: adaptation of superphylum Patescibacteria to groundwater environment drives their genome simplicity.

BackgroundThe newly defined superphylum Patescibacteria such as Parcubacteria (OD1) and Microgenomates (OP11) has been found to be prevalent in groundwater, sediment, lake, and other aquifer environments. Recently increasing attention has been paid to this diverse superphylum including > 20 candidate phyla (a large part of the candidate phylum radiation, CPR) because it refreshed our view of the tree of life. However, adaptive traits contributing to its prevalence are still not well known.ResultsHere, we investigated the genomic features and metabolic pathways of Patescibacteria in groundwater through genome-resolved metagenomics analysis of > 600 Gbp sequence data. We observed that, while the members of Patescibacteria have reduced genomes (~ 1 Mbp) exclusively, functions essential to growth and reproduction such as genetic information processing were retained. Surprisingly, they have sharply reduced redundant and nonessential functions, including specific metabolic activities and stress response systems. The Patescibacteria have ultra-small cells and simplified membrane structures, including flagellar assembly, transporters, and two-component systems. Despite the lack of CRISPR viral defense, the bacteria may evade predation through deletion of common membrane phage receptors and other alternative strategies, which may explain the low representation of prophage proteins in their genomes and lack of CRISPR. By establishing the linkages between bacterial features and the groundwater environmental conditions, our results provide important insights into the functions and evolution of this CPR group.ConclusionsWe found that Patescibacteria has streamlined many functions while acquiring advantages such as avoiding phage invasion, to adapt to the groundwater environment. The unique features of small genome size, ultra-small cell size, and lacking CRISPR of this large lineage are bringing new understandings on life of Bacteria. Our results provide important insights into the mechanisms for adaptation of the superphylum in the groundwater environments, and demonstrate a case where less is more, and small is mighty

Map Supplements for The Metallic Woodboring Beetles (Coleoptera: Buprestidae) of Tennessee

Following compilation of distribution records and seasonal flight activity information for 106 metallic wood-boring beetle (Coleoptera: Buprestidae) species for Tennessee, maps were updated to provide county-level collection notations for 10,543 published records and specimen collections made from 1934 to 2013. County collection tallies across the ecoregions in Tennessee are also presented. Maps for individual species highlight gaps in key geographic areas where specimens have not been collected and can be valuable for future biosurveillance, monitoring and management efforts for these economically and ecologically important insects

Generation and Validation of a Shewanella oneidensis MR-1 Clone Set for Protein Expression and Phage Display

A comprehensive gene collection for S. oneidensis was constructed using the lambda recombinase (Gateway) cloning system. A total of 3584 individual ORFs (85%) have been successfully cloned into the entry plasmids. To validate the use of the clone set, three sets of ORFs were examined within three different destination vectors constructed in this study. Success rates for heterologous protein expression of S. oneidensis His- or His/GST- tagged proteins in E. coli were approximately 70%. The ArcA and NarP transcription factor proteins were tested in an in vitro binding assay to demonstrate that functional proteins can be successfully produced using the clone set. Further functional validation of the clone set was obtained from phage display experiments in which a phage encoding thioredoxin was successfully isolated from a pool of 80 different clones after three rounds of biopanning using immobilized anti-thioredoxin antibody as a target. This clone set complements existing genomic (e.g., whole-genome microarray) and other proteomic tools (e.g., mass spectrometry-based proteomic analysis), and facilitates a wide variety of integrated studies, including protein expression, purification, and functional analyses of proteins both in vivo and in vitro

Is biotechnology (more) acceptable when it enables a reduction in phytosanitary treatments? A European comparison of the acceptability of transgenesis and cisgenesis

Reduced pesticide use is one of the reasons given by Europeans for accepting new genetic engineering techniques. According to the advocates of these techniques, consumers are likely to embrace the application of cisgenesis to apple trees. In order to verify the acceptability of these techniques, we estimate a Bayesian multilevel structural equation model, which takes into account the multidimensional nature of acceptability and individual, national, and European effects, using data from the Eurobarometer 2010 73.1 on science. The results underline the persistence of clear differences between European countries and whilst showing considerable defiance, a relatively wider acceptability of vertical gene transfer as a means of reducing phytosanitary treatments, compared to horizontal transfer

Physical Controls on Salmon Redd Site Selection in Restored Reaches of a Regulated, Gravel‐Bed River

Abstract: Large‐scale river restoration programs have emerged recently as a tool for improving spawning habitat for native salmonids in highly altered river ecosystems. Few studies have quantified the extent to which restored habitat is utilized by salmonids, which habitat features influence redd site selection, or the persistence of restored habitat over time. We investigated fall‐run Chinook salmon spawning site utilization and measured and modeled corresponding habitat characteristics in two restored reaches: a reach of channel and floodplain enhancement completed in 2013 and a reconfigured channel and floodplain constructed in 2002. Redd surveys demonstrated that both restoration projects supported a high density of salmon redds, 3 and 14 years following restoration. Salmon redds were constructed in coarse gravel substrates located in areas of high sediment mobility, as determined by measurements of gravel friction angles and a grain entrainment model. Salmon redds were located near transitions between pool‐riffle bedforms in regions of high predicted hyporheic flows. Habitat quality (quantified as a function of stream hydraulics) and hyporheic flow were both strong predictors of redd occurrence, though the relative roles of these variables differed between sites. Our findings indicate that physical controls on redd site selection in restored channels were similar to those reported for natural channels elsewhere. Our results further highlight that in addition to traditional habitat criteria (e.g., water depth, velocity, and substrate size), quantifying sediment texture and mobility, as well as intragravel flow, provides a more complete understanding of the ecological benefits provided by river restoration projects

Bed load transport and boundary roughness changes as competing causes of hysteresis in the relationship between river discharge and seismic amplitude recorded near a steep mountain stream

International audienceHysteresis in the relationship between bed load transport and river stage is a well-documented phenomenon with multiple known causes. Consequently, numerous studies have interpreted hysteresis in the relationship between seismic ground motion near rivers and some measure of flow strength (i.e., discharge or stage) as the signature of bed load transport. Here we test this hypothesis in the Erlenbach stream (Swiss Prealps) using a metric to quantitatively compare hysteresis in seismic data with hysteresis recorded by geophones attached beneath steel plates within the streambed, a well-calibrated proxy for direct sediment transport measurements. We find that while both the geophones and seismometers demonstrate hysteresis, the magnitude and direction of hysteresis are not significantly correlated between these data, indicating that the seismic signal at this site is primarily reflecting hysteresis in processes other than sediment transport. Seismic hysteresis also does not correlate significantly with the magnitude of sediment transport recorded by the geophones, contrary to previous studies' assumptions. We suggest that hydrologic sources and changes in water turbulence, for instance due to evolving boundary conditions at the bed, rather than changes in sediment transport rates, may sometimes contribute to or even dominate the hysteresis observed in seismic amplitudes near steep mountain rivers. Plain Language SummaryAn increasing number of studies have recently observed changes in the amount of seismic shaking (hysteresis) recorded near a river at a given discharge during floods. Most studies have assumed that this hysteresis was caused by changes in the amount of sediment being transported in the river and have therefore used the hysteresis to assess sediment transport rates and patterns. We examine concurrent seismic and sediment transport data from a steep mountain stream in the Swiss Prealps and find that changes in seismic shaking are unrelated and even opposed (increasing versus decreasing) to changes in sediment transport rates for four out of five transport events. Water turbulence, rather than sediment transport, appears to be the strongest source of seismic shaking, and changes in seismic shaking are most likely caused by changes in turbulence or how turbulence transmits energy through the river bed. These effects may be due to rearrangement of sediment around large boulders on the bed or slight shifting of the boulders themselves. Our results have significant implications for the growing field of fluvial seismology and the evaluation of seismic data near rivers, as previous interpretations of seismic hysteresis as evidence for sediment transport may not always be accurate