Characterisation of mixed virus infections in Ribes species in Switzerland

Various virus disease-like symptoms are frequently observed in Ribes sp. in Switzerland but the aetiology remains poorly documented, although a number of viruses infecting Ribes sp. were described elsewhere. Therefore, symptomatic and apparently healthy plants from diverse origins were analysed by electron microscopy (EM), immunoprecipitation electron microscopy (IPEM), Western blot and (RT-)PCR. By EM, at least four different particle types, often in combination, were observed. (1) Bacilliform particles were typical for the Badnavirus genus with dimensions of 145 x 28 nm. This virus was identified by PCR as the Gooseberry vein banding associated virus (GVBaV). (2) Filamentous particles were mainly observed on black currants with downward rolling of leaves with interveinal reddening during summer and fall. We tentatively named this unknown virus Blackcurrant leafroll-associated virus 1 (BCLRaV-1). In phylogenetic analysis of HSP70h nucleotide sequences, BCLRaV-1 felt in the Closterovirus genus. In Western blot analysis, one dominant protein with an estimated molecular weight of about 28 kDa was detectable. The virus was shown to be different from the Raspberry mottle closterovirus (RMoV) by IPEM and RT-PCR. (3) RTPCR and sequencing of products also clearly demonstrated the presence in our Ribes samples of Rubus chlorotic mottle virus (RuCMV), a Sobemovirus recently described in Scotland. This finding correlates with the presence of the 30 nm diameter particles observed by EM. (4) A further structure with isometrical particles of 60 nm could not yet be attributed to a particular genus. Altogether, our data suggest the presence of multiple virus infections i

Taille de la population d’Avahi laniger dans la réserve d’Ambodiriana-Manompana, Nord-est de Madagascar

Avahi laniger est le seul lémurien nocturne appartenant à la famille des Indriidae qui habite les forêts humides de l’est de Madagascar (Mittermeier et. al., 2010) dont une partie disparaît chaque année (exploitation du bois, pratique du «tavy» ou culture sur brûlis) (Beaucent and Fayolle, 2011; Lehman and Wright, 2000). La fragmentation et la destruction de leur habitat ainsi que la chasse menacent la survie de nombreuses espèces de lémuriens incluant celle de A. laniger (Jenkins et. al., 2011; Rakotondravony and Rabenandrasana, 2011; Anderson, Rowcliffe and Cowlishaw, 2007). Nous avons réalisé, entre fin Avril et Mai 2012, une étude de densité de la population de A. laniger au sein de l’aire protégée de Manompana-Ambodiriana afin d’estimer la taille de la population totale et de déterminer l’impact du projet de conservation menée par l’Association de Défense de la Forêt d’Ambodiriana (ADEFA) qui recherche l’évolution démographique à moyen terme de cette espèce."LABEX" TULIP: (ANR-10-LABX-41), fct fellowship: (SFRH/BD/64875/2009)

The Joint Crisis Plan: A Powerful Tool to Promote Mental Health.

Purpose: The Joint Crisis Plan (JCP) has received growing interest in clinical and research settings. JCP is a type of psychiatric advance statement that describes how to recognize early signs of crisis and how to manage crises. The purpose of the present study, to our knowledge the first to be conducted on this topic in the French-speaking context and to include inpatients, was to describe the content of JCPs and how they are perceived by patients and the providers. Methods: The study used an exploratory, mixed, sequential method. Existing JCPs were retrospectively collected in several clinical contexts (hospital, community settings, and sheltered accommodation). Based on their analyses, we conducted semi-structured interviews including some rating scales on the perception of the JCPs among patients and providers in these settings. For the qualitative analyses, content analyses were conducted with a hybrid approach using NVivo 12 software. Data were double-coded and discussed with a third researcher until agreement was reached. Results: One hundred eighty-four JCPs were collected retrospectively and 24 semi-structured interviews were conducted with 12 patients and 12 providers. No relatives could be included in the research process. The content of the studied JCPs was relevant and indicated that patients had good knowledge of themselves and their illness. Improvements in the quality of the therapeutic relationship, respect for patients' choices and wishes, and a greater sense of control of their illness were reported. The JCP was perceived as a very useful tool by patients and providers. Concerning JCP limitations, lack of staff training, difficulties with the shared decision-making process, and the poor availability of the JCPs when needed were reported. Conclusion: The study highlights that JCPs may be used with patients suffering from a large variety of psychiatric disorders in different care settings. The JCP is perceived as very useful by both patients and providers. The promising results of this study support the promotion of the wide use of JCPs with patients who have experienced crises. It is important to continue to research JCPs through impact studies that include family members

A Barren Landscape?

We consider the generation of a non-perturbative superpotential in F-theory compactifications with flux. We derive a necessary condition for the generation of such a superpotential in F-theory. For models with a single volume modulus, we show that the volume modulus is never stabilized by either abelian instantons or gaugino condensation. We then comment on how our analysis extends to a larger class of compactifications. From our results, it appears that among large volume string compactifications, metastable de Sitter vacua (should any exist) are non-generic.Comment: 14 pages, comments adde

Complete Calabi-Yau metrics from Kahler metrics in D=4

In the present work the local form of certain Calabi-Yau metrics possessing a local Hamiltonian Killing vector is described in terms of a single non linear equation. The main assumptions are that the complex $(3,0)$-form is of the form $e^{ik}\widetilde{\Psi}$, where $\widetilde{\Psi}$ is preserved by the Killing vector, and that the space of the orbits of the Killing vector is, for fixed value of the momentum map coordinate, a complex 4-manifold, in such a way that the complex structure of the 4-manifold is part of the complex structure of the complex 3-fold. The link with the solution generating techniques of [26]-[28] is made explicit and in particular an example with holonomy exactly SU(3) is found by use of the linearization of [26], which was found in the context of D6 branes wrapping a holomorphic 1-fold in a hyperkahler manifold. But the main improvement of the present method, unlike the ones presented in [26]-[28], does not rely in an initial hyperkahler structure. Additionally the complications when dealing with non linear operators over the curved hyperkahler space are avoided by use of this method.Comment: Version accepted for publication in Phys.Rev.

Caged Gammarus fossarum (crustacea) as a robust tool for the characterization of bioavailable contamination levels in continental waters. Toward the determination of threshold values

We investigated the suitability of an active biomonitoring approach, using the ecologically relevant species Gammarus fossarum, to assess trends of bioavailable contamination in continental waters. Gammarids were translocated into cages at 27 sites, in the Rhône-Alpes region (France) during early autumn 2009. Study sites were chosen to represent different physico-chemical characteristics and various anthropic pressures. Biotic factors such as sex, weight and food availability were controlled in order to provide robust and comparable results. After one week of exposure, concentrations of 11 metals/metalloids (Cd, Pb, Hg, Ni, Zn, Cr, Co, Cu, As, Se and Ag) and 38 hydrophobic organic substances including polycyclic aromatic hydrocarbons (PAHs), polychlorobiphenyles (PCBs), pentabromodiphenylethers (PBDEs) and organochlorine pesticides, were measured in gammarids. All metals except Ag, and 33 organic substances among 38 were quantified in G. fossarum, showing that this species is relevant for chemical biomonitoring. The control of biotic factors allowed a robust and direct inter-site comparison of the bioavailable contamination levels. Overall, our results show the interest and robustness of the proposed methodological approach for assessing trends of bioavailable contamination, notably for metals and hydrophobic organic contaminants, in continental waters. Furthermore, we built threshold values of bioavailable contamination in gammarids, above which measured concentrations are expected to reveal a bioavailable contamination at the sampling site. Two ways to define such values were investigated, a statistical approach and a model fit. Threshold values were determined for almost all the substances investigated in this study and similar values were generally derived from the two approaches. Then, levels of contaminants measured in G. fossarum at the 27 study were compared to the threshold values obtained using the model fit. These threshold values could serve as a basis for further implementation of quality grids to rank sites according to the extent of the bioavailable contamination, with regard to the applied methodology

Remarks on evolution of space-times in 3+1 and 4+1 dimensions

A large class of vacuum space-times is constructed in dimension 4+1 from hyperboloidal initial data sets which are not small perturbations of empty space data. These space-times are future geodesically complete, smooth up to their future null infinity, and extend as vacuum space-times through their Cauchy horizon. Dimensional reduction gives non-vacuum space-times with the same properties in 3+1 dimensions.Comment: 10pp, exposition improved; final versio

A Reilly formula and eigenvalue estimates for differential forms

We derive a Reilly-type formula for differential p-forms on a compact manifold with boundary and apply it to give a sharp lower bound of the spectrum of the Hodge Laplacian acting on differential forms of an embedded hypersurface of a Riemannian manifold. The equality case of our inequality gives rise to a number of rigidity results, when the geometry of the boundary has special properties and the domain is non-negatively curved. Finally we also obtain, as a by-product of our calculations, an upper bound of the first eigenvalue of the Hodge Laplacian when the ambient manifold supports non-trivial parallel forms.Comment: 22 page